Experimental composite resin with myristyltrimethylammonium bromide (MYTAB) and alpha-tricalcium phosphate (α-TCP): antibacterial and remineralizing effect
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/271819 |
Resumo: | The aim of this study was to develop an experimental composite resin with the addition of myristyltrimethylammonium bromide (MYTAB) and α -tricalcium phosphate (α-TCP) as an antibac terial and remineralizing material. Experimental composite resins composed of 75 wt% Bisphenol A-Glycidyl Methacrylate (BisGMA) and 25 wt% Triethylene Glycol Dimethacrylate (TEGDMA) were produced. Some 1 mol% Trimethyl benzoyl-diphenylphosphine oxide (TPO) was used as a pho toinitiator, and butylated hydroxytoluene (BTH) was added as a polymerization inhibitor. Silica (1.5 wt%) and barium glass (65 wt%) particles were added as inorganic fillers. For remineralizing and antibacterial effect, α-TCP (10 wt%) and MYTAB (5 wt%) were incorporated into the resin matrix (α-TCP/MYTAB group). A group without the addition of α-TCP/MYTAB was used as a control. Resins were evaluated for their degree of conversion (n = 3) by Fourier Transform Infrared Spec troscopy (FTIR). The flexural strength (n = 5) was assessed based on ISO 4049:2019 requirements. Microhardness was assessed to calculate softening in solvent (n = 3) after ethanol immersion. The mineral deposition (n = 3) was evaluated after immersion in SBF, while cytotoxicity was tested with HaCaT cells (n = 5). Antimicrobial activity (n = 3) was analyzed against S. mutans. The degree of conversion was not influenced by the antibacterial and remineralizing compounds, and all groups reached values > 60%. The α-TCP/MYTAB addition promoted increased softening of polymers after immersion in ethanol and reduced their flexural strength and the viability of cells in vitro. A reduction in S. mutans viability was observed for the α-TCP/MYTAB group in biofilm formation and planktonic bacteria, with an antibacterial effect > 3log10 for the developed materials. Higher intensity of phosphate compounds on the sample’s surface was detected in the α-TCP/MYTAB group The addition of α-TCP and MYTAB promoted remineralizing and antibacterial effects on the developed resins and may be a strategy for bioactive composites |
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Pontons Melo, Juan CarlosBalbinot, Gabriela de SouzaSauro, SalvatoreCollares, Fabrício Mezzomo2024-02-09T05:08:33Z20232079-4983http://hdl.handle.net/10183/271819001176050The aim of this study was to develop an experimental composite resin with the addition of myristyltrimethylammonium bromide (MYTAB) and α -tricalcium phosphate (α-TCP) as an antibac terial and remineralizing material. Experimental composite resins composed of 75 wt% Bisphenol A-Glycidyl Methacrylate (BisGMA) and 25 wt% Triethylene Glycol Dimethacrylate (TEGDMA) were produced. Some 1 mol% Trimethyl benzoyl-diphenylphosphine oxide (TPO) was used as a pho toinitiator, and butylated hydroxytoluene (BTH) was added as a polymerization inhibitor. Silica (1.5 wt%) and barium glass (65 wt%) particles were added as inorganic fillers. For remineralizing and antibacterial effect, α-TCP (10 wt%) and MYTAB (5 wt%) were incorporated into the resin matrix (α-TCP/MYTAB group). A group without the addition of α-TCP/MYTAB was used as a control. Resins were evaluated for their degree of conversion (n = 3) by Fourier Transform Infrared Spec troscopy (FTIR). The flexural strength (n = 5) was assessed based on ISO 4049:2019 requirements. Microhardness was assessed to calculate softening in solvent (n = 3) after ethanol immersion. The mineral deposition (n = 3) was evaluated after immersion in SBF, while cytotoxicity was tested with HaCaT cells (n = 5). Antimicrobial activity (n = 3) was analyzed against S. mutans. The degree of conversion was not influenced by the antibacterial and remineralizing compounds, and all groups reached values > 60%. The α-TCP/MYTAB addition promoted increased softening of polymers after immersion in ethanol and reduced their flexural strength and the viability of cells in vitro. A reduction in S. mutans viability was observed for the α-TCP/MYTAB group in biofilm formation and planktonic bacteria, with an antibacterial effect > 3log10 for the developed materials. Higher intensity of phosphate compounds on the sample’s surface was detected in the α-TCP/MYTAB group The addition of α-TCP and MYTAB promoted remineralizing and antibacterial effects on the developed resins and may be a strategy for bioactive compositesapplication/pdfporJournal of Functional Biomaterials. Basel. Vol. 14, no. 6, (2023), 303, 12 p.Resinas compostasCárie dentáriaStreptococcus mutansAntibacterianosComposite resinDental cariesStreptococcus mutansAntibacterial agentsBioactive materialsExperimental composite resin with myristyltrimethylammonium bromide (MYTAB) and alpha-tricalcium phosphate (α-TCP): antibacterial and remineralizing effectEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001176050.pdf.txt001176050.pdf.txtExtracted Texttext/plain50297http://www.lume.ufrgs.br/bitstream/10183/271819/2/001176050.pdf.txtd2cda419753cfb8643f6f11c2f2af355MD52ORIGINAL001176050.pdfTexto completo (inglês)application/pdf2728934http://www.lume.ufrgs.br/bitstream/10183/271819/1/001176050.pdf1c74b847d2e8a2de837a824c9b36d6caMD5110183/2718192024-02-10 06:09:42.058179oai:www.lume.ufrgs.br:10183/271819Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2024-02-10T08:09:42Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Experimental composite resin with myristyltrimethylammonium bromide (MYTAB) and alpha-tricalcium phosphate (α-TCP): antibacterial and remineralizing effect |
title |
Experimental composite resin with myristyltrimethylammonium bromide (MYTAB) and alpha-tricalcium phosphate (α-TCP): antibacterial and remineralizing effect |
spellingShingle |
Experimental composite resin with myristyltrimethylammonium bromide (MYTAB) and alpha-tricalcium phosphate (α-TCP): antibacterial and remineralizing effect Pontons Melo, Juan Carlos Resinas compostas Cárie dentária Streptococcus mutans Antibacterianos Composite resin Dental caries Streptococcus mutans Antibacterial agents Bioactive materials |
title_short |
Experimental composite resin with myristyltrimethylammonium bromide (MYTAB) and alpha-tricalcium phosphate (α-TCP): antibacterial and remineralizing effect |
title_full |
Experimental composite resin with myristyltrimethylammonium bromide (MYTAB) and alpha-tricalcium phosphate (α-TCP): antibacterial and remineralizing effect |
title_fullStr |
Experimental composite resin with myristyltrimethylammonium bromide (MYTAB) and alpha-tricalcium phosphate (α-TCP): antibacterial and remineralizing effect |
title_full_unstemmed |
Experimental composite resin with myristyltrimethylammonium bromide (MYTAB) and alpha-tricalcium phosphate (α-TCP): antibacterial and remineralizing effect |
title_sort |
Experimental composite resin with myristyltrimethylammonium bromide (MYTAB) and alpha-tricalcium phosphate (α-TCP): antibacterial and remineralizing effect |
author |
Pontons Melo, Juan Carlos |
author_facet |
Pontons Melo, Juan Carlos Balbinot, Gabriela de Souza Sauro, Salvatore Collares, Fabrício Mezzomo |
author_role |
author |
author2 |
Balbinot, Gabriela de Souza Sauro, Salvatore Collares, Fabrício Mezzomo |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Pontons Melo, Juan Carlos Balbinot, Gabriela de Souza Sauro, Salvatore Collares, Fabrício Mezzomo |
dc.subject.por.fl_str_mv |
Resinas compostas Cárie dentária Streptococcus mutans Antibacterianos |
topic |
Resinas compostas Cárie dentária Streptococcus mutans Antibacterianos Composite resin Dental caries Streptococcus mutans Antibacterial agents Bioactive materials |
dc.subject.eng.fl_str_mv |
Composite resin Dental caries Streptococcus mutans Antibacterial agents Bioactive materials |
description |
The aim of this study was to develop an experimental composite resin with the addition of myristyltrimethylammonium bromide (MYTAB) and α -tricalcium phosphate (α-TCP) as an antibac terial and remineralizing material. Experimental composite resins composed of 75 wt% Bisphenol A-Glycidyl Methacrylate (BisGMA) and 25 wt% Triethylene Glycol Dimethacrylate (TEGDMA) were produced. Some 1 mol% Trimethyl benzoyl-diphenylphosphine oxide (TPO) was used as a pho toinitiator, and butylated hydroxytoluene (BTH) was added as a polymerization inhibitor. Silica (1.5 wt%) and barium glass (65 wt%) particles were added as inorganic fillers. For remineralizing and antibacterial effect, α-TCP (10 wt%) and MYTAB (5 wt%) were incorporated into the resin matrix (α-TCP/MYTAB group). A group without the addition of α-TCP/MYTAB was used as a control. Resins were evaluated for their degree of conversion (n = 3) by Fourier Transform Infrared Spec troscopy (FTIR). The flexural strength (n = 5) was assessed based on ISO 4049:2019 requirements. Microhardness was assessed to calculate softening in solvent (n = 3) after ethanol immersion. The mineral deposition (n = 3) was evaluated after immersion in SBF, while cytotoxicity was tested with HaCaT cells (n = 5). Antimicrobial activity (n = 3) was analyzed against S. mutans. The degree of conversion was not influenced by the antibacterial and remineralizing compounds, and all groups reached values > 60%. The α-TCP/MYTAB addition promoted increased softening of polymers after immersion in ethanol and reduced their flexural strength and the viability of cells in vitro. A reduction in S. mutans viability was observed for the α-TCP/MYTAB group in biofilm formation and planktonic bacteria, with an antibacterial effect > 3log10 for the developed materials. Higher intensity of phosphate compounds on the sample’s surface was detected in the α-TCP/MYTAB group The addition of α-TCP and MYTAB promoted remineralizing and antibacterial effects on the developed resins and may be a strategy for bioactive composites |
publishDate |
2023 |
dc.date.issued.fl_str_mv |
2023 |
dc.date.accessioned.fl_str_mv |
2024-02-09T05:08:33Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/271819 |
dc.identifier.issn.pt_BR.fl_str_mv |
2079-4983 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001176050 |
identifier_str_mv |
2079-4983 001176050 |
url |
http://hdl.handle.net/10183/271819 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.ispartof.pt_BR.fl_str_mv |
Journal of Functional Biomaterials. Basel. Vol. 14, no. 6, (2023), 303, 12 p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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