Correlation between neurotrophic activity and sensitivity to mechanical stimuli in immobilization stress model treated with transcranial direct current stimulation
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Tipo de documento: | Trabalho de conclusão de curso |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/240924 |
Resumo: | Stress causes biochemical and behavioral changes that can contribute to the genesis of diseases. Transcranial direct current stimulation modulates neuronal excitability. In the present study, the hypothesis that there is a correlation between the effects of tDCS on neurotrophic activity and the ability to increase the threshold for mechanical sensitivity was tested. Male Wistar rats were divided into ten groups: 1) Behavioral control (BeC); 2) Biochemical control (BiC); 3) Immobilization 30 (IMO30); 4) Immobilization 60 (IMO60); 5) Immobilization 120 (IMO120); 6) Immobilization 24h (IMO24h); 7) Immobilization + tDCS 30 (IMO+tDCS30); 8) Immobilization + tDCS 60 (IMO+tDCS60); 9) Immobilization + tDCS 120 (IMO+tDCS120); 10) Immobilization + tDCS 24h (IMO+tDCS24). Afterwards, they were submitted to the von Frey baseline behavioral test. In the following six days, they were submitted to the stress model by immobilization. On the seventh day, they received the last immobilization with the application of 20 minutes of tDCS or false stimulation with disconnected electrodes. Immediately after, they were subjected to the von Frey test 30, 60, 120 minutes and 24 hours after the session, then killed by decapitation with spinal cord collection for biochemical analysis. BDNF levels cord were assessed by Enzyme Linked Immunosorbent Assay (ELISA). There was a negative correlation between BDNF levels and mechanical sensitivity. The result show that tDCS can be an option to prevent effects of stress, however, studies still need to define adequate doses that the increase in BDNF is not exacerbated to the point of reducing the nociceptive threshold. |
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Silva, Carolini daQuevedo, Alexandre Silva deFernandes, Elissa Kerli2022-06-24T04:43:25Z2020http://hdl.handle.net/10183/240924001125616Stress causes biochemical and behavioral changes that can contribute to the genesis of diseases. Transcranial direct current stimulation modulates neuronal excitability. In the present study, the hypothesis that there is a correlation between the effects of tDCS on neurotrophic activity and the ability to increase the threshold for mechanical sensitivity was tested. Male Wistar rats were divided into ten groups: 1) Behavioral control (BeC); 2) Biochemical control (BiC); 3) Immobilization 30 (IMO30); 4) Immobilization 60 (IMO60); 5) Immobilization 120 (IMO120); 6) Immobilization 24h (IMO24h); 7) Immobilization + tDCS 30 (IMO+tDCS30); 8) Immobilization + tDCS 60 (IMO+tDCS60); 9) Immobilization + tDCS 120 (IMO+tDCS120); 10) Immobilization + tDCS 24h (IMO+tDCS24). Afterwards, they were submitted to the von Frey baseline behavioral test. In the following six days, they were submitted to the stress model by immobilization. On the seventh day, they received the last immobilization with the application of 20 minutes of tDCS or false stimulation with disconnected electrodes. Immediately after, they were subjected to the von Frey test 30, 60, 120 minutes and 24 hours after the session, then killed by decapitation with spinal cord collection for biochemical analysis. BDNF levels cord were assessed by Enzyme Linked Immunosorbent Assay (ELISA). There was a negative correlation between BDNF levels and mechanical sensitivity. The result show that tDCS can be an option to prevent effects of stress, however, studies still need to define adequate doses that the increase in BDNF is not exacerbated to the point of reducing the nociceptive threshold.application/pdfengFarmáciaEstimulação transcraniana por corrente contínuaEstresseBDNFtDCSTranscranial direct current stimulationCorrelation between neurotrophic activity and sensitivity to mechanical stimuli in immobilization stress model treated with transcranial direct current stimulationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisUniversidade Federal do Rio Grande do SulFaculdade de FarmáciaPorto Alegre, BR-RS2020Farmáciagraduaçãoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001125616.pdf.txt001125616.pdf.txtExtracted Texttext/plain27434http://www.lume.ufrgs.br/bitstream/10183/240924/2/001125616.pdf.txt2a84ee55f4a1b5431559915273811633MD52ORIGINAL001125616.pdfTexto completo (inglês)application/pdf403646http://www.lume.ufrgs.br/bitstream/10183/240924/1/001125616.pdfc50a3fa6e8ab29b67e233ed7bbab54c7MD5110183/2409242022-07-07 04:58:36.739001oai:www.lume.ufrgs.br:10183/240924Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2022-07-07T07:58:36Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Correlation between neurotrophic activity and sensitivity to mechanical stimuli in immobilization stress model treated with transcranial direct current stimulation |
title |
Correlation between neurotrophic activity and sensitivity to mechanical stimuli in immobilization stress model treated with transcranial direct current stimulation |
spellingShingle |
Correlation between neurotrophic activity and sensitivity to mechanical stimuli in immobilization stress model treated with transcranial direct current stimulation Silva, Carolini da Farmácia Estimulação transcraniana por corrente contínua Estresse BDNF tDCS Transcranial direct current stimulation |
title_short |
Correlation between neurotrophic activity and sensitivity to mechanical stimuli in immobilization stress model treated with transcranial direct current stimulation |
title_full |
Correlation between neurotrophic activity and sensitivity to mechanical stimuli in immobilization stress model treated with transcranial direct current stimulation |
title_fullStr |
Correlation between neurotrophic activity and sensitivity to mechanical stimuli in immobilization stress model treated with transcranial direct current stimulation |
title_full_unstemmed |
Correlation between neurotrophic activity and sensitivity to mechanical stimuli in immobilization stress model treated with transcranial direct current stimulation |
title_sort |
Correlation between neurotrophic activity and sensitivity to mechanical stimuli in immobilization stress model treated with transcranial direct current stimulation |
author |
Silva, Carolini da |
author_facet |
Silva, Carolini da |
author_role |
author |
dc.contributor.author.fl_str_mv |
Silva, Carolini da |
dc.contributor.advisor1.fl_str_mv |
Quevedo, Alexandre Silva de |
dc.contributor.advisor-co1.fl_str_mv |
Fernandes, Elissa Kerli |
contributor_str_mv |
Quevedo, Alexandre Silva de Fernandes, Elissa Kerli |
dc.subject.por.fl_str_mv |
Farmácia Estimulação transcraniana por corrente contínua Estresse |
topic |
Farmácia Estimulação transcraniana por corrente contínua Estresse BDNF tDCS Transcranial direct current stimulation |
dc.subject.eng.fl_str_mv |
BDNF tDCS Transcranial direct current stimulation |
description |
Stress causes biochemical and behavioral changes that can contribute to the genesis of diseases. Transcranial direct current stimulation modulates neuronal excitability. In the present study, the hypothesis that there is a correlation between the effects of tDCS on neurotrophic activity and the ability to increase the threshold for mechanical sensitivity was tested. Male Wistar rats were divided into ten groups: 1) Behavioral control (BeC); 2) Biochemical control (BiC); 3) Immobilization 30 (IMO30); 4) Immobilization 60 (IMO60); 5) Immobilization 120 (IMO120); 6) Immobilization 24h (IMO24h); 7) Immobilization + tDCS 30 (IMO+tDCS30); 8) Immobilization + tDCS 60 (IMO+tDCS60); 9) Immobilization + tDCS 120 (IMO+tDCS120); 10) Immobilization + tDCS 24h (IMO+tDCS24). Afterwards, they were submitted to the von Frey baseline behavioral test. In the following six days, they were submitted to the stress model by immobilization. On the seventh day, they received the last immobilization with the application of 20 minutes of tDCS or false stimulation with disconnected electrodes. Immediately after, they were subjected to the von Frey test 30, 60, 120 minutes and 24 hours after the session, then killed by decapitation with spinal cord collection for biochemical analysis. BDNF levels cord were assessed by Enzyme Linked Immunosorbent Assay (ELISA). There was a negative correlation between BDNF levels and mechanical sensitivity. The result show that tDCS can be an option to prevent effects of stress, however, studies still need to define adequate doses that the increase in BDNF is not exacerbated to the point of reducing the nociceptive threshold. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020 |
dc.date.accessioned.fl_str_mv |
2022-06-24T04:43:25Z |
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