Overcoming dormancy and determining optimal temperature for slender serradella seed germination

• Autor(a) principal: Lopes, Rodrigo Ramos
• Data de Publicação: 2015
• Outros Autores: Souza, Cleber Henrique Lopes de, Bertoncelli, Patricia, Franke, Lucia Brandao
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFRGS
• Texto Completo: http://hdl.handle.net/10183/147249
• topic: Melhoramento genetico animal; Reprodução animal; Fertilidade; Touro; Germination rate; Ornithopus pinnatus; Scarification; Synchronization index of germination

The objective of this study was to identify the most efficient method for overcoming coat-imposed dormancy and determine the optimal germination temperature for Ornithopus pinnatus seeds. Treatments to overcome dormancy were: intact seeds; immersion in hot water at 60 ºC, followed by soaking in the same water (unheated)/24 h; immersion in hot water at 90 ºC, followed by soaking in the same water (unheated)/24 h; mechanical scarification; chemical scarification, H2SO4/5 min; and chemical scarification, H2SO4/10 min. Percentages were calculated for germinated, abnormal, dormant, and dead seeds. Optimal germination temperatures were calculated using eight constant temperatures (0, 5, 10, 15, 20, 25, 30, and 35 °C), with 8 h of light and 16 h of darkness. Germination rate, frequency, and synchronization index of germination were used as parameters to establish optimum temperature for germination. A completely randomized experimental design was used, with twelve repetitions of 100 seeds per treatment. Data obtained were subjected to analysis of variance and Tukey’s test at a 5% significance level. O. pinnatus seeds exhibit dormancy caused by the seed coat’s impermeability to water. Mechanical scarification was efficient in promoting seed germination. The species is stenothermal, meaning it cannot tolerate significant temperature variations. The germination rate of seeds was linearly dependent on temperature. Synchronization of the germination process is greater in the optimum temperature range, established between 15 and 20 °C.