Behavior of Salmonella heidelberg and salmonella enteritidis strains following broiler chick inoculation: evaluation of cecal morphometry, liver and cecum bacterial counts and fecal excretion patterns
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/30522 |
Resumo: | Over the years, Salmonella Heidelberg (SH) has gained prominence in North America poultry production and in the poultry production of other countries. Salmonella Heidelberg has been isolated and reported from poultry and poultry products in Brazil since 1962, whereas Salmonella Enteritidis (SE) has only emerged as a serious problem in poultry and public health since 1993. These strains of Salmonella can cause intestinal problems in newly hatched chicks, and infection may persist until adulthood. Upon slaughter of chickens, Salmonella can contaminate carcasses, a condition that poses a threat to human health. The aim of this study was to compare the fecal excretion of Salmonella Enteritidis and Salmonella Heidelberg in newly hatched chicks (orally inoculated with 105ufc/mL each) until 20 days of age. In addition, the ratio of cecal villus height:crypt depth (morphometry) and liver and cecum cell counts was analyzed in chicks ranging from 0 to 3 days of age and infected with these two Salmonella strains. One hundred seventeen chicks were separated into one of three experimental groups: a control group, an SEinfected group and an SH-infected group. Eight chicks per group were euthanized at 6, 12 and 72 hours post-inoculation (pi) to allow for Salmonella isolation from the liver and cecum and for the collection of the cecum for villi and crypt analysis. Other birds were allowed to mature to 20 days of age and cloacal swabs were taken at 2, 6, 13 and 20 days pi to compare the fecal excretion of inoculated strains. The Salmonella Enteritidis group had a higher number of cells excreted during the trial. Both strains were isolated from the liver and cecum by 6h pi. At 12h pi the Salmonella Heidelberg group had high cell counts in the cecum. No difference was found in liver cell counts. Both strains showed lower villus height:crypt depth ratio than the control group post-infection. |
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Borsoi, AnderliseSantos, Luciana Ruschel dosRodrigues, Laura BeatrizMoraes, Hamilton Luiz de SouzaSalle, Carlos Tadeu PippiNascimento, Vladimir Pinheiro do2011-08-04T06:01:05Z20111517-8382http://hdl.handle.net/10183/30522000768078Over the years, Salmonella Heidelberg (SH) has gained prominence in North America poultry production and in the poultry production of other countries. Salmonella Heidelberg has been isolated and reported from poultry and poultry products in Brazil since 1962, whereas Salmonella Enteritidis (SE) has only emerged as a serious problem in poultry and public health since 1993. These strains of Salmonella can cause intestinal problems in newly hatched chicks, and infection may persist until adulthood. Upon slaughter of chickens, Salmonella can contaminate carcasses, a condition that poses a threat to human health. The aim of this study was to compare the fecal excretion of Salmonella Enteritidis and Salmonella Heidelberg in newly hatched chicks (orally inoculated with 105ufc/mL each) until 20 days of age. In addition, the ratio of cecal villus height:crypt depth (morphometry) and liver and cecum cell counts was analyzed in chicks ranging from 0 to 3 days of age and infected with these two Salmonella strains. One hundred seventeen chicks were separated into one of three experimental groups: a control group, an SEinfected group and an SH-infected group. Eight chicks per group were euthanized at 6, 12 and 72 hours post-inoculation (pi) to allow for Salmonella isolation from the liver and cecum and for the collection of the cecum for villi and crypt analysis. Other birds were allowed to mature to 20 days of age and cloacal swabs were taken at 2, 6, 13 and 20 days pi to compare the fecal excretion of inoculated strains. The Salmonella Enteritidis group had a higher number of cells excreted during the trial. Both strains were isolated from the liver and cecum by 6h pi. At 12h pi the Salmonella Heidelberg group had high cell counts in the cecum. No difference was found in liver cell counts. Both strains showed lower villus height:crypt depth ratio than the control group post-infection.application/pdfengBrazilian journal of microbiology. São Paulo, SP. Vol. 42, n. 1 (jan./mar. 2011), p. 266-273Salmonella : AvesSalmonella HeidelbergSalmonella enteritidisSalmonella HeidelbergSalmonella enteritidisFecal ExcretionVillus: crypt ratioBroilerBehavior of Salmonella heidelberg and salmonella enteritidis strains following broiler chick inoculation: evaluation of cecal morphometry, liver and cecum bacterial counts and fecal excretion patternsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000768078.pdf.txt000768078.pdf.txtExtracted Texttext/plain28277http://www.lume.ufrgs.br/bitstream/10183/30522/2/000768078.pdf.txt21683f777d66cdfdaac3f714d861c2f4MD52ORIGINAL000768078.pdf000768078.pdfTexto completo (inglês)application/pdf328096http://www.lume.ufrgs.br/bitstream/10183/30522/1/000768078.pdf77f7691cc9e68148597d7bd4f491036cMD51THUMBNAIL000768078.pdf.jpg000768078.pdf.jpgGenerated Thumbnailimage/jpeg1856http://www.lume.ufrgs.br/bitstream/10183/30522/3/000768078.pdf.jpg061d58cb4d4f716b73456ff040ea4f90MD5310183/305222018-10-09 09:29:12.467oai:www.lume.ufrgs.br:10183/30522Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-09T12:29:12Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Behavior of Salmonella heidelberg and salmonella enteritidis strains following broiler chick inoculation: evaluation of cecal morphometry, liver and cecum bacterial counts and fecal excretion patterns |
title |
Behavior of Salmonella heidelberg and salmonella enteritidis strains following broiler chick inoculation: evaluation of cecal morphometry, liver and cecum bacterial counts and fecal excretion patterns |
spellingShingle |
Behavior of Salmonella heidelberg and salmonella enteritidis strains following broiler chick inoculation: evaluation of cecal morphometry, liver and cecum bacterial counts and fecal excretion patterns Borsoi, Anderlise Salmonella : Aves Salmonella Heidelberg Salmonella enteritidis Salmonella Heidelberg Salmonella enteritidis Fecal Excretion Villus: crypt ratio Broiler |
title_short |
Behavior of Salmonella heidelberg and salmonella enteritidis strains following broiler chick inoculation: evaluation of cecal morphometry, liver and cecum bacterial counts and fecal excretion patterns |
title_full |
Behavior of Salmonella heidelberg and salmonella enteritidis strains following broiler chick inoculation: evaluation of cecal morphometry, liver and cecum bacterial counts and fecal excretion patterns |
title_fullStr |
Behavior of Salmonella heidelberg and salmonella enteritidis strains following broiler chick inoculation: evaluation of cecal morphometry, liver and cecum bacterial counts and fecal excretion patterns |
title_full_unstemmed |
Behavior of Salmonella heidelberg and salmonella enteritidis strains following broiler chick inoculation: evaluation of cecal morphometry, liver and cecum bacterial counts and fecal excretion patterns |
title_sort |
Behavior of Salmonella heidelberg and salmonella enteritidis strains following broiler chick inoculation: evaluation of cecal morphometry, liver and cecum bacterial counts and fecal excretion patterns |
author |
Borsoi, Anderlise |
author_facet |
Borsoi, Anderlise Santos, Luciana Ruschel dos Rodrigues, Laura Beatriz Moraes, Hamilton Luiz de Souza Salle, Carlos Tadeu Pippi Nascimento, Vladimir Pinheiro do |
author_role |
author |
author2 |
Santos, Luciana Ruschel dos Rodrigues, Laura Beatriz Moraes, Hamilton Luiz de Souza Salle, Carlos Tadeu Pippi Nascimento, Vladimir Pinheiro do |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Borsoi, Anderlise Santos, Luciana Ruschel dos Rodrigues, Laura Beatriz Moraes, Hamilton Luiz de Souza Salle, Carlos Tadeu Pippi Nascimento, Vladimir Pinheiro do |
dc.subject.por.fl_str_mv |
Salmonella : Aves Salmonella Heidelberg Salmonella enteritidis |
topic |
Salmonella : Aves Salmonella Heidelberg Salmonella enteritidis Salmonella Heidelberg Salmonella enteritidis Fecal Excretion Villus: crypt ratio Broiler |
dc.subject.eng.fl_str_mv |
Salmonella Heidelberg Salmonella enteritidis Fecal Excretion Villus: crypt ratio Broiler |
description |
Over the years, Salmonella Heidelberg (SH) has gained prominence in North America poultry production and in the poultry production of other countries. Salmonella Heidelberg has been isolated and reported from poultry and poultry products in Brazil since 1962, whereas Salmonella Enteritidis (SE) has only emerged as a serious problem in poultry and public health since 1993. These strains of Salmonella can cause intestinal problems in newly hatched chicks, and infection may persist until adulthood. Upon slaughter of chickens, Salmonella can contaminate carcasses, a condition that poses a threat to human health. The aim of this study was to compare the fecal excretion of Salmonella Enteritidis and Salmonella Heidelberg in newly hatched chicks (orally inoculated with 105ufc/mL each) until 20 days of age. In addition, the ratio of cecal villus height:crypt depth (morphometry) and liver and cecum cell counts was analyzed in chicks ranging from 0 to 3 days of age and infected with these two Salmonella strains. One hundred seventeen chicks were separated into one of three experimental groups: a control group, an SEinfected group and an SH-infected group. Eight chicks per group were euthanized at 6, 12 and 72 hours post-inoculation (pi) to allow for Salmonella isolation from the liver and cecum and for the collection of the cecum for villi and crypt analysis. Other birds were allowed to mature to 20 days of age and cloacal swabs were taken at 2, 6, 13 and 20 days pi to compare the fecal excretion of inoculated strains. The Salmonella Enteritidis group had a higher number of cells excreted during the trial. Both strains were isolated from the liver and cecum by 6h pi. At 12h pi the Salmonella Heidelberg group had high cell counts in the cecum. No difference was found in liver cell counts. Both strains showed lower villus height:crypt depth ratio than the control group post-infection. |
publishDate |
2011 |
dc.date.accessioned.fl_str_mv |
2011-08-04T06:01:05Z |
dc.date.issued.fl_str_mv |
2011 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/30522 |
dc.identifier.issn.pt_BR.fl_str_mv |
1517-8382 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000768078 |
identifier_str_mv |
1517-8382 000768078 |
url |
http://hdl.handle.net/10183/30522 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian journal of microbiology. São Paulo, SP. Vol. 42, n. 1 (jan./mar. 2011), p. 266-273 |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
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