Technique of Palynomorphs Extraction from Dental Calculi of South American Gomphotheres

Detalhes bibliográficos
Autor(a) principal: Misumi, Shana Yuri
Data de Publicação: 2017
Outros Autores: Asevedo, Lidiane, Avilla, Leonardo dos Santos, Barth, Ortrud Monika, Aguiar de, Marcia Barros
Tipo de documento: Artigo
Idioma: por
Título da fonte: Anuário do Instituto de Geociências (Online)
Texto Completo: https://revistas.ufrj.br/index.php/aigeo/article/view/7868
Resumo: A laboratory technique was developed to extract palynomorphs from dental calculi of South American gomphotere Notiomastodon platensis individuals. The samples were obtained from molar specimens from Northeastern and Southern Brazil. The laboratory procedure consists in steps of maceration, addition of Lycopodium clavatum exotic spores, reaction with concentrated hydrochloric acid, washing with distilled water and sonication. Glycerin gelatin was used to prepare the microscope slides containing the microfossils. Pollen grains, pteridophyte spores and fragments of plant tissues were recovered. This methodology allows the extraction and observation of other microfossils, such as phytoliths, that might also be present in dental calculi of herbivorous mammals.
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spelling Technique of Palynomorphs Extraction from Dental Calculi of South American GomphotheresTécnica de Extração de Palinomorfos em Cálculos Déntarios de Gonfotérios Sul-AmericanosDental calculus, South American gomphotheres; Palynomorphs; Extraction techniqueCálculo dentário; Gonfotérios sul-americanos; Palinomorfos; Técnica de extraçãoA laboratory technique was developed to extract palynomorphs from dental calculi of South American gomphotere Notiomastodon platensis individuals. The samples were obtained from molar specimens from Northeastern and Southern Brazil. The laboratory procedure consists in steps of maceration, addition of Lycopodium clavatum exotic spores, reaction with concentrated hydrochloric acid, washing with distilled water and sonication. Glycerin gelatin was used to prepare the microscope slides containing the microfossils. Pollen grains, pteridophyte spores and fragments of plant tissues were recovered. This methodology allows the extraction and observation of other microfossils, such as phytoliths, that might also be present in dental calculi of herbivorous mammals.Foi desenvolvida uma técnica de laboratório para a extração de palinomorfos de cálculos dentários de indivíduos do gonfotério sul-americano Notiomastodon platensis. As amostras foram retiradas de espécimes molares provenientes de localidades do Nordeste e do Sul do Brasil. O procedimento constitui-se em etapas de maceração, adição de esporos exóticos de Lycopodium clavatum , reação com ácido clorídrico concentrado, lavagem com água destilada e tamisação por ultrassom. A preparação das lâminas com os palinomorfos obtidos fez uso de gelatina glicerinada. Foram recuperados grãos de pólen, esporos de pteridófitas e fragmentos de tecidos vegetais. Esta metodologia permite a extração e observação de outros microfósseis, como fitólitos, que também podem estar presentes em cálculos dentários de mamíferos herbívoros.Universidade Federal do Rio de Janeiro2017-02-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistas.ufrj.br/index.php/aigeo/article/view/786810.11137/2016_1_127_132Anuário do Instituto de Geociências; Vol 39, No 1 (2016); 127-132Anuário do Instituto de Geociências; Vol 39, No 1 (2016); 127-1321982-39080101-9759reponame:Anuário do Instituto de Geociências (Online)instname:Universidade Federal do Rio de Janeiro (UFRJ)instacron:UFRJporhttps://revistas.ufrj.br/index.php/aigeo/article/view/7868/6349Copyright (c) 2016 Anuário do Instituto de Geociênciashttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessMisumi, Shana YuriAsevedo, LidianeAvilla, Leonardo dos SantosBarth, Ortrud MonikaAguiar de, Marcia Barros2017-02-15T18:21:34Zoai:www.revistas.ufrj.br:article/7868Revistahttps://revistas.ufrj.br/index.php/aigeo/indexPUBhttps://revistas.ufrj.br/index.php/aigeo/oaianuario@igeo.ufrj.br||1982-39080101-9759opendoar:2017-02-15T18:21:34Anuário do Instituto de Geociências (Online) - Universidade Federal do Rio de Janeiro (UFRJ)false
dc.title.none.fl_str_mv Technique of Palynomorphs Extraction from Dental Calculi of South American Gomphotheres
Técnica de Extração de Palinomorfos em Cálculos Déntarios de Gonfotérios Sul-Americanos
title Technique of Palynomorphs Extraction from Dental Calculi of South American Gomphotheres
spellingShingle Technique of Palynomorphs Extraction from Dental Calculi of South American Gomphotheres
Misumi, Shana Yuri
Dental calculus, South American gomphotheres; Palynomorphs; Extraction technique
Cálculo dentário; Gonfotérios sul-americanos; Palinomorfos; Técnica de extração
title_short Technique of Palynomorphs Extraction from Dental Calculi of South American Gomphotheres
title_full Technique of Palynomorphs Extraction from Dental Calculi of South American Gomphotheres
title_fullStr Technique of Palynomorphs Extraction from Dental Calculi of South American Gomphotheres
title_full_unstemmed Technique of Palynomorphs Extraction from Dental Calculi of South American Gomphotheres
title_sort Technique of Palynomorphs Extraction from Dental Calculi of South American Gomphotheres
author Misumi, Shana Yuri
author_facet Misumi, Shana Yuri
Asevedo, Lidiane
Avilla, Leonardo dos Santos
Barth, Ortrud Monika
Aguiar de, Marcia Barros
author_role author
author2 Asevedo, Lidiane
Avilla, Leonardo dos Santos
Barth, Ortrud Monika
Aguiar de, Marcia Barros
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Misumi, Shana Yuri
Asevedo, Lidiane
Avilla, Leonardo dos Santos
Barth, Ortrud Monika
Aguiar de, Marcia Barros
dc.subject.por.fl_str_mv Dental calculus, South American gomphotheres; Palynomorphs; Extraction technique
Cálculo dentário; Gonfotérios sul-americanos; Palinomorfos; Técnica de extração
topic Dental calculus, South American gomphotheres; Palynomorphs; Extraction technique
Cálculo dentário; Gonfotérios sul-americanos; Palinomorfos; Técnica de extração
description A laboratory technique was developed to extract palynomorphs from dental calculi of South American gomphotere Notiomastodon platensis individuals. The samples were obtained from molar specimens from Northeastern and Southern Brazil. The laboratory procedure consists in steps of maceration, addition of Lycopodium clavatum exotic spores, reaction with concentrated hydrochloric acid, washing with distilled water and sonication. Glycerin gelatin was used to prepare the microscope slides containing the microfossils. Pollen grains, pteridophyte spores and fragments of plant tissues were recovered. This methodology allows the extraction and observation of other microfossils, such as phytoliths, that might also be present in dental calculi of herbivorous mammals.
publishDate 2017
dc.date.none.fl_str_mv 2017-02-15
dc.type.none.fl_str_mv

dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv https://revistas.ufrj.br/index.php/aigeo/article/view/7868
10.11137/2016_1_127_132
url https://revistas.ufrj.br/index.php/aigeo/article/view/7868
identifier_str_mv 10.11137/2016_1_127_132
dc.language.iso.fl_str_mv por
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dc.relation.none.fl_str_mv https://revistas.ufrj.br/index.php/aigeo/article/view/7868/6349
dc.rights.driver.fl_str_mv Copyright (c) 2016 Anuário do Instituto de Geociências
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2016 Anuário do Instituto de Geociências
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal do Rio de Janeiro
publisher.none.fl_str_mv Universidade Federal do Rio de Janeiro
dc.source.none.fl_str_mv Anuário do Instituto de Geociências; Vol 39, No 1 (2016); 127-132
Anuário do Instituto de Geociências; Vol 39, No 1 (2016); 127-132
1982-3908
0101-9759
reponame:Anuário do Instituto de Geociências (Online)
instname:Universidade Federal do Rio de Janeiro (UFRJ)
instacron:UFRJ
instname_str Universidade Federal do Rio de Janeiro (UFRJ)
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institution UFRJ
reponame_str Anuário do Instituto de Geociências (Online)
collection Anuário do Instituto de Geociências (Online)
repository.name.fl_str_mv Anuário do Instituto de Geociências (Online) - Universidade Federal do Rio de Janeiro (UFRJ)
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