Isolamento e cultivo de cianobactérias potencialmente tóxicas de açudes do Semiárido/RN

• Autor(a) principal: Andrade, Thállyn Dávila dos Santos de
• Data de Publicação: 2016
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UFRN
• Texto Completo: https://repositorio.ufrn.br/handle/123456789/47262
• topic: Cianobactéria; Cyanobacteria; Microcistina; Microcystin; Isolamento e Cultivo; Isolation and Cultivation

Cyanobacteria are prokaryotic microorganisms, photosynthetic capable of developing blooms in eutrophic environments and producing cyanotoxins that can be neurotoxic, hepatotoxic and dermatotoxic. These can infect domestic and wild animals, except man, representing a serious risk to health. Studies show that toxic algal blooms are common in reservoirs of the Potiguar semiarid. This study aims to isolate and maintain a bank of single-species cultures of cyanobacteria Potiguar semiarid to support research in Aquatic Microbiology Laboratory - LAMAq. The samples were collected in July/2015 and March/2016, including the state of extreme drought and rain, respectively, in the Dam Engineer Armando Ribeiro Gonçalves and weir Boqueirão. The collection was carried out with the aid of a vertical trawl with 20μm mesh opening for qualitative analysis and isolation. Part of this sample was fixed in 4% formalin for subsequent identification of the species and the rest was kept in vivo for subsequent isolation. For quantitative analysis the sample was collected with bottle of Van Dorn and fixed with acetic lugol for further analysis by Utermöhl method. The evaluation of the toxicity microcystin was performed by ELISA using commercial kits. The isolation was done by seeding on a solid medium and isolating fishery in liquid medium with optical microscope. The cultures are maintained in medium ASM- 1 and ASM- 1 with double iron photoperiod of 12 hours at a temperature of 24 ± 2 ° C and light intensity of 80-1700 LUX. Eight species of cyanobacteria were identified, Cylindrspermopsis raciborskii, Geitlerinema unigranulatum, Anabaena planctonica, Anabaena circinalis, Microcystis panniformis and Microcystis aeruginosa present in Boqueirão, Coelomorom Tropicali, Planktothrix agardhii, Cylindrospermopsis raciborskii and Geitlerinema unigranulatum in Dam Engineer Armando Ribeiro Gonçalves. The species Cylindrospermopsis raciborskii and Geitlerinema unigranulatum were present in both environments. There was a predominance of Anabaena planctonica the weir Boqueirao and Planktothrix agardhii in Dam Engineer Armando Ribeiro Gonçalves. Three strains were isolated , Microcystis aeruginosa , Anabaena circinalis and planktonic Anabaena , which showed higher growth in ASM- 1 medium with bent iron concentration and are held in LAMAq. Moreover, it detected the presence of microcystin, by ELISA, the following concentrations: 3.1 μg/L for Weir Boqueirão; 3.3 μg/L for the Dam Eng Armando Ribeiro Gonçalves; 2.8 μg/L for strains of Microcystis aeruginosa ; 2.6 μg/L for strains isolated from Anabaena planctonica and 2.8 μg/L for strains isolated from Anabaena circinalis .