In vitro digestibility of globulins from cowpea (vigna unguiculata) and xerophitic algaroba (prosopis juliflora) seeds by mammalian digestive proteinases: a comparative study
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Publication Date: | 2002 |
Other Authors: | , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UFRN |
Download full: | https://repositorio.ufrn.br/handle/123456789/58147 https://doi.org/10.1016/S0308-8146(01)00391-0 |
Summary: | Globulins were purified from mature and immature cowpea and algaroba seeds by gel filtration (S-200), anion exchange (DEAE-Sepharose) and other chromatographic methods. These globulins (native and heated) were submitted to hydrolysis by pepsin, chymotrypsin and trypsin and their digestion products were analysed by SDS-PAGE. Results showed that native globulins of both legumes were weakly digested by pepsin and were not digested by serine proteases. Heated proteins from cowpea were digested more rapidly by pepsin and chymotrypsin than was algaroba globulin. Trypsin rapidly digested proteins from both cowpea and algaroba. Comparing the hydrolysis of bean globulins to the algaroba globulins by mammalian enzymes, the globulins from immature cowpea showed better digestibility than mature cowpea; globulins from algaroba pod, which is used as an alternative food, were difficult to digest |
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Morais, Ana Heloneida de AraújoOliveira, AdelianaLima, LizianePereira, RaileneCardoso, PatríciaMiranda, Maria RaquelSales, MaurícioXavier Filho, José2024-04-12T22:35:27Z2024-04-12T22:35:27Z2002MORAIS, Ana Heloneida Araújo; OLIVEIRA, Adeliana; LIMA, Liziane; PEREIRA, Railene; CARDOSO, Patrícia; MIRANDA, Maria Raquel; SALES, Maurício; XAVIER FILHO, José. In vitro digestibility of globulins from cowpea (vigna unguiculata) and xerophitic algaroba (prosopis juliflora) seeds by mammalian digestive proteinases: a comparative study. Food Chemistry, [S.l.], v. 78, p. 143-147, 2002. Disponível em: https://www.sciencedirect.com/science/article/pii/S0308814601003910?via%3Dihub.https://repositorio.ufrn.br/handle/123456789/58147https://doi.org/10.1016/S0308-8146(01)00391-0Food ChemistryProsopisVignaLegumeGlobulinsDigestibilityIn vitro digestibility of globulins from cowpea (vigna unguiculata) and xerophitic algaroba (prosopis juliflora) seeds by mammalian digestive proteinases: a comparative studyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGlobulins were purified from mature and immature cowpea and algaroba seeds by gel filtration (S-200), anion exchange (DEAE-Sepharose) and other chromatographic methods. These globulins (native and heated) were submitted to hydrolysis by pepsin, chymotrypsin and trypsin and their digestion products were analysed by SDS-PAGE. Results showed that native globulins of both legumes were weakly digested by pepsin and were not digested by serine proteases. Heated proteins from cowpea were digested more rapidly by pepsin and chymotrypsin than was algaroba globulin. Trypsin rapidly digested proteins from both cowpea and algaroba. Comparing the hydrolysis of bean globulins to the algaroba globulins by mammalian enzymes, the globulins from immature cowpea showed better digestibility than mature cowpea; globulins from algaroba pod, which is used as an alternative food, were difficult to digestengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNinfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/58147/2/license.txte9597aa2854d128fd968be5edc8a28d9MD52123456789/581472024-04-12 19:35:49.077oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2024-04-12T22:35:49Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
In vitro digestibility of globulins from cowpea (vigna unguiculata) and xerophitic algaroba (prosopis juliflora) seeds by mammalian digestive proteinases: a comparative study |
title |
In vitro digestibility of globulins from cowpea (vigna unguiculata) and xerophitic algaroba (prosopis juliflora) seeds by mammalian digestive proteinases: a comparative study |
spellingShingle |
In vitro digestibility of globulins from cowpea (vigna unguiculata) and xerophitic algaroba (prosopis juliflora) seeds by mammalian digestive proteinases: a comparative study Morais, Ana Heloneida de Araújo Prosopis Vigna Legume Globulins Digestibility |
title_short |
In vitro digestibility of globulins from cowpea (vigna unguiculata) and xerophitic algaroba (prosopis juliflora) seeds by mammalian digestive proteinases: a comparative study |
title_full |
In vitro digestibility of globulins from cowpea (vigna unguiculata) and xerophitic algaroba (prosopis juliflora) seeds by mammalian digestive proteinases: a comparative study |
title_fullStr |
In vitro digestibility of globulins from cowpea (vigna unguiculata) and xerophitic algaroba (prosopis juliflora) seeds by mammalian digestive proteinases: a comparative study |
title_full_unstemmed |
In vitro digestibility of globulins from cowpea (vigna unguiculata) and xerophitic algaroba (prosopis juliflora) seeds by mammalian digestive proteinases: a comparative study |
title_sort |
In vitro digestibility of globulins from cowpea (vigna unguiculata) and xerophitic algaroba (prosopis juliflora) seeds by mammalian digestive proteinases: a comparative study |
author |
Morais, Ana Heloneida de Araújo |
author_facet |
Morais, Ana Heloneida de Araújo Oliveira, Adeliana Lima, Liziane Pereira, Railene Cardoso, Patrícia Miranda, Maria Raquel Sales, Maurício Xavier Filho, José |
author_role |
author |
author2 |
Oliveira, Adeliana Lima, Liziane Pereira, Railene Cardoso, Patrícia Miranda, Maria Raquel Sales, Maurício Xavier Filho, José |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Morais, Ana Heloneida de Araújo Oliveira, Adeliana Lima, Liziane Pereira, Railene Cardoso, Patrícia Miranda, Maria Raquel Sales, Maurício Xavier Filho, José |
dc.subject.por.fl_str_mv |
Prosopis Vigna Legume Globulins Digestibility |
topic |
Prosopis Vigna Legume Globulins Digestibility |
description |
Globulins were purified from mature and immature cowpea and algaroba seeds by gel filtration (S-200), anion exchange (DEAE-Sepharose) and other chromatographic methods. These globulins (native and heated) were submitted to hydrolysis by pepsin, chymotrypsin and trypsin and their digestion products were analysed by SDS-PAGE. Results showed that native globulins of both legumes were weakly digested by pepsin and were not digested by serine proteases. Heated proteins from cowpea were digested more rapidly by pepsin and chymotrypsin than was algaroba globulin. Trypsin rapidly digested proteins from both cowpea and algaroba. Comparing the hydrolysis of bean globulins to the algaroba globulins by mammalian enzymes, the globulins from immature cowpea showed better digestibility than mature cowpea; globulins from algaroba pod, which is used as an alternative food, were difficult to digest |
publishDate |
2002 |
dc.date.issued.fl_str_mv |
2002 |
dc.date.accessioned.fl_str_mv |
2024-04-12T22:35:27Z |
dc.date.available.fl_str_mv |
2024-04-12T22:35:27Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MORAIS, Ana Heloneida Araújo; OLIVEIRA, Adeliana; LIMA, Liziane; PEREIRA, Railene; CARDOSO, Patrícia; MIRANDA, Maria Raquel; SALES, Maurício; XAVIER FILHO, José. In vitro digestibility of globulins from cowpea (vigna unguiculata) and xerophitic algaroba (prosopis juliflora) seeds by mammalian digestive proteinases: a comparative study. Food Chemistry, [S.l.], v. 78, p. 143-147, 2002. Disponível em: https://www.sciencedirect.com/science/article/pii/S0308814601003910?via%3Dihub. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/handle/123456789/58147 |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1016/S0308-8146(01)00391-0 |
identifier_str_mv |
MORAIS, Ana Heloneida Araújo; OLIVEIRA, Adeliana; LIMA, Liziane; PEREIRA, Railene; CARDOSO, Patrícia; MIRANDA, Maria Raquel; SALES, Maurício; XAVIER FILHO, José. In vitro digestibility of globulins from cowpea (vigna unguiculata) and xerophitic algaroba (prosopis juliflora) seeds by mammalian digestive proteinases: a comparative study. Food Chemistry, [S.l.], v. 78, p. 143-147, 2002. Disponível em: https://www.sciencedirect.com/science/article/pii/S0308814601003910?via%3Dihub. |
url |
https://repositorio.ufrn.br/handle/123456789/58147 https://doi.org/10.1016/S0308-8146(01)00391-0 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Food Chemistry |
publisher.none.fl_str_mv |
Food Chemistry |
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Repositório Institucional da UFRN |
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