Expressão de zif268 no cérebro do lagarto Tropidurus Hispidus após exploração de um ambiente enriquecido.
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/jspui/handle/123456789/17291 |
Resumo: | In the present work, we investigated behavioral changes associated with the increase in Zif268 protein expression within telencephalic areas of the tropical lizard Tropidurus hispidus that correspond to the mammalian hippocampus (HC). We used 13 male individuals of this species, collected at the Federal Agrotechnical School of Rio Grande do Norte, under SISBIO license number 19561-1. Four animals had their brains removed and were submitted to a Western blot with antibodies for the Zif268 protein. The remaining animals were separated in two different groups: a control group (n=4) and an exploration group (n=5). Animals from the exploration group were exposed to an enriched environment with many sensory cues novel to them. Control group animals stayed in the environment they were already habituated to. After 90 min from the onset of exposure to the new environment, animals from both groups were submitted to intracardiac perfusion with fixative, and the brains were removed, cryoprotected and frozen. After that, brains were sectioned at 20 μm and the sections were subjected to immunohistochemistry for the Zif268 protein. We verified that the Zif268 protein is likely conserved in the brain of T. hispidus, which showed antigenicity for the antibody anti-Zif268 made in mammals. In animals from the exploration group, we detected an increase of the Zif268 protein in the Septum, Striatum, Dorsoventricular Area and in cortical areas corresponding to the HC. This increase was proportional to the amount of environmental exploration, with maximum positive correlation in the hippocampal subareas Medial Cortex (R = 0.94 and p = 0.004) and Dorsomedial Cortex (R = 0.92 and p = 0.006). The data corroborate the notion that the reptilian hippocampus, as well as the mammalian HC, plays an important role in spatial exploration. |
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Santos, José Ronaldo doshttp://lattes.cnpq.br/3626754379892089http://lattes.cnpq.br/0649912135067700Silva, Regina Helena dahttp://lattes.cnpq.br/0101190051087933Marchioro, Murilohttp://lattes.cnpq.br/0236515445675827Ribeiro, Sidarta Tollendal Gomes2014-12-17T15:36:58Z2010-06-102014-12-17T15:36:58Z2010-02-24SANTOS, José Ronaldo dos. Expressão de zif268 no cérebro do lagarto Tropidurus Hispidus após exploração de um ambiente enriquecido.. 2010. 84 f. Dissertação (Mestrado em Estudos de Comportamento; Psicologia Fisiológica) - Universidade Federal do Rio Grande do Norte, Natal, 2010.https://repositorio.ufrn.br/jspui/handle/123456789/17291In the present work, we investigated behavioral changes associated with the increase in Zif268 protein expression within telencephalic areas of the tropical lizard Tropidurus hispidus that correspond to the mammalian hippocampus (HC). We used 13 male individuals of this species, collected at the Federal Agrotechnical School of Rio Grande do Norte, under SISBIO license number 19561-1. Four animals had their brains removed and were submitted to a Western blot with antibodies for the Zif268 protein. The remaining animals were separated in two different groups: a control group (n=4) and an exploration group (n=5). Animals from the exploration group were exposed to an enriched environment with many sensory cues novel to them. Control group animals stayed in the environment they were already habituated to. After 90 min from the onset of exposure to the new environment, animals from both groups were submitted to intracardiac perfusion with fixative, and the brains were removed, cryoprotected and frozen. After that, brains were sectioned at 20 μm and the sections were subjected to immunohistochemistry for the Zif268 protein. We verified that the Zif268 protein is likely conserved in the brain of T. hispidus, which showed antigenicity for the antibody anti-Zif268 made in mammals. In animals from the exploration group, we detected an increase of the Zif268 protein in the Septum, Striatum, Dorsoventricular Area and in cortical areas corresponding to the HC. This increase was proportional to the amount of environmental exploration, with maximum positive correlation in the hippocampal subareas Medial Cortex (R = 0.94 and p = 0.004) and Dorsomedial Cortex (R = 0.92 and p = 0.006). The data corroborate the notion that the reptilian hippocampus, as well as the mammalian HC, plays an important role in spatial exploration.Neste trabalho, foram investigadas alterações comportamentais associadas ao aumento da expressão da proteína Zif268 em áreas telencefálicas do lagarto tropical Tropidurus hispidus correspondentes ao Hipocampo (HC) de mamíferos. Foram utilizados 13 animais machos do lagarto T. hispidus, coletados no campus da Escola Agrotécnica Federal do RN, sob a licença do SISBIO (n.19561-1). Quatro animais tiveram seus cérebros removidos a fresco e submetidos a Western blot com anticorpo para a proteína Zif268. Os animais restantes foram separados em dois grupos distintos, grupo controle (n=4) e grupo exploração (n=5). Animais do grupo exploração foram expostos a um Ambiente Enriquecido (AE) com diversas pistas espaciais desconhecidas pelos animais. Os animais do grupo controle permaneceram no ambiente ao qual já estavam previamente habituados. Transcorridos 90min do início da exposição ao ambiente, animais de ambos os grupos, foram submetidos a perfusão intracardíaca com fixador, e os cérebros foram removidos crioprotegidos e congelados. Posteriormente os cérebros foram seccionados a 20μm e submetidos à imunohistoquímica para Zif268. Verificamos a conservação da proteína Zif268 no cérebro do T. hispidus, com antigenicidade para o anticorpo anti-Zif268 produzido em mamíferos. Nos lagartos do grupo exploração detectou-se aumento da expressão da proteína Zif268 no Septo, Estriado, Área Dorsoventricular e áreas corticais que correspondem ao HC (Córtices Medial CM, Dorsal CD, Dorsomedial CDM). Esse aumento é proporcional à exploração do ambiente novo, com máxima correlação nas subáreas hipocampais do grupo exploração, Córtex Medial (R = 0,94 e p = 0,004) e Dorsomedial (R = 0,92 e p = 0,006). Os dados corroboram a noção de que o hipocampo reptiliano, assim como o HC de mamíferos, desempenham um papel importante na exploração de novos ambientes.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal do Rio Grande do NortePrograma de Pós-Graduação em PsicobiologiaUFRNBREstudos de Comportamento; Psicologia FisiológicaExpressão de Zif268plasticidadeambiente enriquecidoRépteis.Expression of zif268plasticityenriched environmentReptile.CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERALExpressão de zif268 no cérebro do lagarto Tropidurus Hispidus após exploração de um ambiente enriquecido.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALJoseRS.pdfapplication/pdf1457742https://repositorio.ufrn.br/bitstream/123456789/17291/1/JoseRS.pdf2f02b1d902f74f6756aecf4c07ded437MD51TEXTJoseRS.pdf.txtJoseRS.pdf.txtExtracted texttext/plain100573https://repositorio.ufrn.br/bitstream/123456789/17291/6/JoseRS.pdf.txt12e94da7cd127a0cd2a38015bfc12579MD56THUMBNAILJoseRS.pdf.jpgJoseRS.pdf.jpgIM Thumbnailimage/jpeg1384https://repositorio.ufrn.br/bitstream/123456789/17291/7/JoseRS.pdf.jpg713d95f4dc7bdf970a827b1d8398a820MD57123456789/172912017-11-04 16:58:41.291oai:https://repositorio.ufrn.br:123456789/17291Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2017-11-04T19:58:41Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.por.fl_str_mv |
Expressão de zif268 no cérebro do lagarto Tropidurus Hispidus após exploração de um ambiente enriquecido. |
title |
Expressão de zif268 no cérebro do lagarto Tropidurus Hispidus após exploração de um ambiente enriquecido. |
spellingShingle |
Expressão de zif268 no cérebro do lagarto Tropidurus Hispidus após exploração de um ambiente enriquecido. Santos, José Ronaldo dos Expressão de Zif268 plasticidade ambiente enriquecido Répteis. Expression of zif268 plasticity enriched environment Reptile. CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
title_short |
Expressão de zif268 no cérebro do lagarto Tropidurus Hispidus após exploração de um ambiente enriquecido. |
title_full |
Expressão de zif268 no cérebro do lagarto Tropidurus Hispidus após exploração de um ambiente enriquecido. |
title_fullStr |
Expressão de zif268 no cérebro do lagarto Tropidurus Hispidus após exploração de um ambiente enriquecido. |
title_full_unstemmed |
Expressão de zif268 no cérebro do lagarto Tropidurus Hispidus após exploração de um ambiente enriquecido. |
title_sort |
Expressão de zif268 no cérebro do lagarto Tropidurus Hispidus após exploração de um ambiente enriquecido. |
author |
Santos, José Ronaldo dos |
author_facet |
Santos, José Ronaldo dos |
author_role |
author |
dc.contributor.authorID.por.fl_str_mv |
|
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/3626754379892089 |
dc.contributor.advisorID.por.fl_str_mv |
|
dc.contributor.advisorLattes.por.fl_str_mv |
http://lattes.cnpq.br/0649912135067700 |
dc.contributor.referees1.pt_BR.fl_str_mv |
Silva, Regina Helena da |
dc.contributor.referees1ID.por.fl_str_mv |
|
dc.contributor.referees1Lattes.por.fl_str_mv |
http://lattes.cnpq.br/0101190051087933 |
dc.contributor.referees2.pt_BR.fl_str_mv |
Marchioro, Murilo |
dc.contributor.referees2ID.por.fl_str_mv |
|
dc.contributor.referees2Lattes.por.fl_str_mv |
http://lattes.cnpq.br/0236515445675827 |
dc.contributor.author.fl_str_mv |
Santos, José Ronaldo dos |
dc.contributor.advisor1.fl_str_mv |
Ribeiro, Sidarta Tollendal Gomes |
contributor_str_mv |
Ribeiro, Sidarta Tollendal Gomes |
dc.subject.por.fl_str_mv |
Expressão de Zif268 plasticidade ambiente enriquecido Répteis. |
topic |
Expressão de Zif268 plasticidade ambiente enriquecido Répteis. Expression of zif268 plasticity enriched environment Reptile. CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
dc.subject.eng.fl_str_mv |
Expression of zif268 plasticity enriched environment Reptile. |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
description |
In the present work, we investigated behavioral changes associated with the increase in Zif268 protein expression within telencephalic areas of the tropical lizard Tropidurus hispidus that correspond to the mammalian hippocampus (HC). We used 13 male individuals of this species, collected at the Federal Agrotechnical School of Rio Grande do Norte, under SISBIO license number 19561-1. Four animals had their brains removed and were submitted to a Western blot with antibodies for the Zif268 protein. The remaining animals were separated in two different groups: a control group (n=4) and an exploration group (n=5). Animals from the exploration group were exposed to an enriched environment with many sensory cues novel to them. Control group animals stayed in the environment they were already habituated to. After 90 min from the onset of exposure to the new environment, animals from both groups were submitted to intracardiac perfusion with fixative, and the brains were removed, cryoprotected and frozen. After that, brains were sectioned at 20 μm and the sections were subjected to immunohistochemistry for the Zif268 protein. We verified that the Zif268 protein is likely conserved in the brain of T. hispidus, which showed antigenicity for the antibody anti-Zif268 made in mammals. In animals from the exploration group, we detected an increase of the Zif268 protein in the Septum, Striatum, Dorsoventricular Area and in cortical areas corresponding to the HC. This increase was proportional to the amount of environmental exploration, with maximum positive correlation in the hippocampal subareas Medial Cortex (R = 0.94 and p = 0.004) and Dorsomedial Cortex (R = 0.92 and p = 0.006). The data corroborate the notion that the reptilian hippocampus, as well as the mammalian HC, plays an important role in spatial exploration. |
publishDate |
2010 |
dc.date.available.fl_str_mv |
2010-06-10 2014-12-17T15:36:58Z |
dc.date.issued.fl_str_mv |
2010-02-24 |
dc.date.accessioned.fl_str_mv |
2014-12-17T15:36:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SANTOS, José Ronaldo dos. Expressão de zif268 no cérebro do lagarto Tropidurus Hispidus após exploração de um ambiente enriquecido.. 2010. 84 f. Dissertação (Mestrado em Estudos de Comportamento; Psicologia Fisiológica) - Universidade Federal do Rio Grande do Norte, Natal, 2010. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/jspui/handle/123456789/17291 |
identifier_str_mv |
SANTOS, José Ronaldo dos. Expressão de zif268 no cérebro do lagarto Tropidurus Hispidus após exploração de um ambiente enriquecido.. 2010. 84 f. Dissertação (Mestrado em Estudos de Comportamento; Psicologia Fisiológica) - Universidade Federal do Rio Grande do Norte, Natal, 2010. |
url |
https://repositorio.ufrn.br/jspui/handle/123456789/17291 |
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por |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Federal do Rio Grande do Norte |
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Programa de Pós-Graduação em Psicobiologia |
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UFRN |
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BR |
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Estudos de Comportamento; Psicologia Fisiológica |
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Universidade Federal do Rio Grande do Norte |
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