Insulin modulates myogenesis and muscle atrophy resulting from skin scald burn in young male rats
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/handle/123456789/31035 |
Resumo: | Burn injuries (BIs) due to scalding are one of the most common accidents among children. BIs greater than 40% of total body surface area are considered extensive and result in local and systemic response. We sought to assess morphological and myogenic mechanisms through both short- and long-term intensive insulin therapies that affect the skeletal muscle after extensive skin BI in young rats. Materials and methods: Wistar rats aged 21 d were distributed into four groups: control (C), control with insulin (C þ I), scald burn injury (SI), and SI with insulin (SI þ I). The SI groups were submitted to a 45% total body surface area burn, and the C þ I and SI þ I groups received insulin (5 UI/Kg/d) for 4 or 14 d. Glucose tolerance and the homeostatic model assessment of insulin resistance index were determined. Gastrocnemius muscles were analyzed for histopathological, morphometric, and immunohistochemical myogenic parameters (Pax7, MyoD, and MyoG); in addition, the expression of genes related to muscle atrophy (MuRF1 and MAFbx) and its regulation (IGF-1) were also assessed. Results: Short-term treatment with insulin favored muscle regeneration by primary myogenesis and decreased muscle atrophy in animals with BIs, whereas the long-term treatment modulated myogenesis by increasing the MyoD protein. Both treatments improved histopathological parameters and secondary myogenesis by increasing the MyoG protein. Conclusions: Treatment with insulin benefits myogenic parameters during regeneration and modulates MuRF1, an important mediator of muscle atrophy |
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Quintana, Hananiah TardivoBaptista, Vivianne Izabelle de AraújoLazzarin, Mariana CruzAntunes, Hanna Karen MoreiraLe Sueur-Maluf, LucianaOliveira, Camila Aparecida Machado deOliveira, Flavia de2020-12-17T14:01:29Z2020-12-17T14:01:29Z2020-08-17QUINTANA, Hananiah Tardivo; BAPTISTA, Vivianne Izabelle de Araújo; LAZZARIN, Mariana Cruz; ANTUNES, Hanna Karen Moreira; LE SUEUR-MALUF, Luciana; OLIVEIRA, Camila Aparecida Machado de; OLIVEIRA, Flavia de. Insulin modulates myogenesis and muscle atrophy resulting from skin scald burn in young male rats. Journal Of Surgical Research, [s. l.], v. 257, p. 56-68, jan. 2021. Disponível em: https://www.journalofsurgicalresearch.com/article/S0022-4804(20)30497-2/fulltext. Acesso em: 03 nov. 2020. http://dx.doi.org/10.1016/j.jss.2020.07.0400022-48041095-8673https://repositorio.ufrn.br/handle/123456789/3103510.1016/j.jss.2020.07.040ElsevierAttribution 3.0 Brazilhttp://creativecommons.org/licenses/by/3.0/br/info:eu-repo/semantics/openAccessBurn injuryInsulinSkeletal muscleMyogenesisAtrophyInsulin modulates myogenesis and muscle atrophy resulting from skin scald burn in young male ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBurn injuries (BIs) due to scalding are one of the most common accidents among children. BIs greater than 40% of total body surface area are considered extensive and result in local and systemic response. We sought to assess morphological and myogenic mechanisms through both short- and long-term intensive insulin therapies that affect the skeletal muscle after extensive skin BI in young rats. Materials and methods: Wistar rats aged 21 d were distributed into four groups: control (C), control with insulin (C þ I), scald burn injury (SI), and SI with insulin (SI þ I). The SI groups were submitted to a 45% total body surface area burn, and the C þ I and SI þ I groups received insulin (5 UI/Kg/d) for 4 or 14 d. Glucose tolerance and the homeostatic model assessment of insulin resistance index were determined. Gastrocnemius muscles were analyzed for histopathological, morphometric, and immunohistochemical myogenic parameters (Pax7, MyoD, and MyoG); in addition, the expression of genes related to muscle atrophy (MuRF1 and MAFbx) and its regulation (IGF-1) were also assessed. Results: Short-term treatment with insulin favored muscle regeneration by primary myogenesis and decreased muscle atrophy in animals with BIs, whereas the long-term treatment modulated myogenesis by increasing the MyoD protein. Both treatments improved histopathological parameters and secondary myogenesis by increasing the MyoG protein. Conclusions: Treatment with insulin benefits myogenic parameters during regeneration and modulates MuRF1, an important mediator of muscle atrophyengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.ufrn.br/bitstream/123456789/31035/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD52ORIGINALInsulinModulatesMyogenesis_Baptista_2020.pdfInsulinModulatesMyogenesis_Baptista_2020.pdfapplication/pdf5337578https://repositorio.ufrn.br/bitstream/123456789/31035/1/InsulinModulatesMyogenesis_Baptista_2020.pdf22c6e258b1968672d79e3ae6acf41078MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/31035/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53TEXTInsulinModulatesMyogenesis_Baptista_2020.pdf.txtInsulinModulatesMyogenesis_Baptista_2020.pdf.txtExtracted texttext/plain48541https://repositorio.ufrn.br/bitstream/123456789/31035/4/InsulinModulatesMyogenesis_Baptista_2020.pdf.txt39987f9a0bd3af53cacc7c9ecc182ea3MD54THUMBNAILInsulinModulatesMyogenesis_Baptista_2020.pdf.jpgInsulinModulatesMyogenesis_Baptista_2020.pdf.jpgGenerated Thumbnailimage/jpeg1837https://repositorio.ufrn.br/bitstream/123456789/31035/5/InsulinModulatesMyogenesis_Baptista_2020.pdf.jpgd6c024f98596c39d010786107d1f8456MD55123456789/310352020-12-20 04:59:31.365oai:https://repositorio.ufrn.br:123456789/31035Tk9OLUVYQ0xVU0lWRSBESVNUUklCVVRJT04gTElDRU5TRQoKCkJ5IHNpZ25pbmcgYW5kIGRlbGl2ZXJpbmcgdGhpcyBsaWNlbnNlLCBNci4gKGF1dGhvciBvciBjb3B5cmlnaHQgaG9sZGVyKToKCgphKSBHcmFudHMgdGhlIFVuaXZlcnNpZGFkZSBGZWRlcmFsIFJpbyBHcmFuZGUgZG8gTm9ydGUgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgb2YKcmVwcm9kdWNlLCBjb252ZXJ0IChhcyBkZWZpbmVkIGJlbG93KSwgY29tbXVuaWNhdGUgYW5kIC8gb3IKZGlzdHJpYnV0ZSB0aGUgZGVsaXZlcmVkIGRvY3VtZW50IChpbmNsdWRpbmcgYWJzdHJhY3QgLyBhYnN0cmFjdCkgaW4KZGlnaXRhbCBvciBwcmludGVkIGZvcm1hdCBhbmQgaW4gYW55IG1lZGl1bS4KCmIpIERlY2xhcmVzIHRoYXQgdGhlIGRvY3VtZW50IHN1Ym1pdHRlZCBpcyBpdHMgb3JpZ2luYWwgd29yaywgYW5kIHRoYXQKeW91IGhhdmUgdGhlIHJpZ2h0IHRvIGdyYW50IHRoZSByaWdodHMgY29udGFpbmVkIGluIHRoaXMgbGljZW5zZS4gRGVjbGFyZXMKdGhhdCB0aGUgZGVsaXZlcnkgb2YgdGhlIGRvY3VtZW50IGRvZXMgbm90IGluZnJpbmdlLCBhcyBmYXIgYXMgaXQgaXMKdGhlIHJpZ2h0cyBvZiBhbnkgb3RoZXIgcGVyc29uIG9yIGVudGl0eS4KCmMpIElmIHRoZSBkb2N1bWVudCBkZWxpdmVyZWQgY29udGFpbnMgbWF0ZXJpYWwgd2hpY2ggZG9lcyBub3QKcmlnaHRzLCBkZWNsYXJlcyB0aGF0IGl0IGhhcyBvYnRhaW5lZCBhdXRob3JpemF0aW9uIGZyb20gdGhlIGhvbGRlciBvZiB0aGUKY29weXJpZ2h0IHRvIGdyYW50IHRoZSBVbml2ZXJzaWRhZGUgRmVkZXJhbCBkbyBSaW8gR3JhbmRlIGRvIE5vcnRlIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdCB0aGlzIG1hdGVyaWFsIHdob3NlIHJpZ2h0cyBhcmUgb2YKdGhpcmQgcGFydGllcyBpcyBjbGVhcmx5IGlkZW50aWZpZWQgYW5kIHJlY29nbml6ZWQgaW4gdGhlIHRleHQgb3IKY29udGVudCBvZiB0aGUgZG9jdW1lbnQgZGVsaXZlcmVkLgoKSWYgdGhlIGRvY3VtZW50IHN1Ym1pdHRlZCBpcyBiYXNlZCBvbiBmdW5kZWQgb3Igc3VwcG9ydGVkIHdvcmsKYnkgYW5vdGhlciBpbnN0aXR1dGlvbiBvdGhlciB0aGFuIHRoZSBVbml2ZXJzaWRhZGUgRmVkZXJhbCBkbyBSaW8gR3JhbmRlIGRvIE5vcnRlLCBkZWNsYXJlcyB0aGF0IGl0IGhhcyBmdWxmaWxsZWQgYW55IG9ibGlnYXRpb25zIHJlcXVpcmVkIGJ5IHRoZSByZXNwZWN0aXZlIGFncmVlbWVudCBvciBhZ3JlZW1lbnQuCgpUaGUgVW5pdmVyc2lkYWRlIEZlZGVyYWwgZG8gUmlvIEdyYW5kZSBkbyBOb3J0ZSB3aWxsIGNsZWFybHkgaWRlbnRpZnkgaXRzIG5hbWUgKHMpIGFzIHRoZSBhdXRob3IgKHMpIG9yIGhvbGRlciAocykgb2YgdGhlIGRvY3VtZW50J3MgcmlnaHRzCmRlbGl2ZXJlZCwgYW5kIHdpbGwgbm90IG1ha2UgYW55IGNoYW5nZXMsIG90aGVyIHRoYW4gdGhvc2UgcGVybWl0dGVkIGJ5CnRoaXMgbGljZW5zZQo=Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2020-12-20T07:59:31Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Insulin modulates myogenesis and muscle atrophy resulting from skin scald burn in young male rats |
title |
Insulin modulates myogenesis and muscle atrophy resulting from skin scald burn in young male rats |
spellingShingle |
Insulin modulates myogenesis and muscle atrophy resulting from skin scald burn in young male rats Quintana, Hananiah Tardivo Burn injury Insulin Skeletal muscle Myogenesis Atrophy |
title_short |
Insulin modulates myogenesis and muscle atrophy resulting from skin scald burn in young male rats |
title_full |
Insulin modulates myogenesis and muscle atrophy resulting from skin scald burn in young male rats |
title_fullStr |
Insulin modulates myogenesis and muscle atrophy resulting from skin scald burn in young male rats |
title_full_unstemmed |
Insulin modulates myogenesis and muscle atrophy resulting from skin scald burn in young male rats |
title_sort |
Insulin modulates myogenesis and muscle atrophy resulting from skin scald burn in young male rats |
author |
Quintana, Hananiah Tardivo |
author_facet |
Quintana, Hananiah Tardivo Baptista, Vivianne Izabelle de Araújo Lazzarin, Mariana Cruz Antunes, Hanna Karen Moreira Le Sueur-Maluf, Luciana Oliveira, Camila Aparecida Machado de Oliveira, Flavia de |
author_role |
author |
author2 |
Baptista, Vivianne Izabelle de Araújo Lazzarin, Mariana Cruz Antunes, Hanna Karen Moreira Le Sueur-Maluf, Luciana Oliveira, Camila Aparecida Machado de Oliveira, Flavia de |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Quintana, Hananiah Tardivo Baptista, Vivianne Izabelle de Araújo Lazzarin, Mariana Cruz Antunes, Hanna Karen Moreira Le Sueur-Maluf, Luciana Oliveira, Camila Aparecida Machado de Oliveira, Flavia de |
dc.subject.por.fl_str_mv |
Burn injury Insulin Skeletal muscle Myogenesis Atrophy |
topic |
Burn injury Insulin Skeletal muscle Myogenesis Atrophy |
description |
Burn injuries (BIs) due to scalding are one of the most common accidents among children. BIs greater than 40% of total body surface area are considered extensive and result in local and systemic response. We sought to assess morphological and myogenic mechanisms through both short- and long-term intensive insulin therapies that affect the skeletal muscle after extensive skin BI in young rats. Materials and methods: Wistar rats aged 21 d were distributed into four groups: control (C), control with insulin (C þ I), scald burn injury (SI), and SI with insulin (SI þ I). The SI groups were submitted to a 45% total body surface area burn, and the C þ I and SI þ I groups received insulin (5 UI/Kg/d) for 4 or 14 d. Glucose tolerance and the homeostatic model assessment of insulin resistance index were determined. Gastrocnemius muscles were analyzed for histopathological, morphometric, and immunohistochemical myogenic parameters (Pax7, MyoD, and MyoG); in addition, the expression of genes related to muscle atrophy (MuRF1 and MAFbx) and its regulation (IGF-1) were also assessed. Results: Short-term treatment with insulin favored muscle regeneration by primary myogenesis and decreased muscle atrophy in animals with BIs, whereas the long-term treatment modulated myogenesis by increasing the MyoD protein. Both treatments improved histopathological parameters and secondary myogenesis by increasing the MyoG protein. Conclusions: Treatment with insulin benefits myogenic parameters during regeneration and modulates MuRF1, an important mediator of muscle atrophy |
publishDate |
2020 |
dc.date.accessioned.fl_str_mv |
2020-12-17T14:01:29Z |
dc.date.available.fl_str_mv |
2020-12-17T14:01:29Z |
dc.date.issued.fl_str_mv |
2020-08-17 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
QUINTANA, Hananiah Tardivo; BAPTISTA, Vivianne Izabelle de Araújo; LAZZARIN, Mariana Cruz; ANTUNES, Hanna Karen Moreira; LE SUEUR-MALUF, Luciana; OLIVEIRA, Camila Aparecida Machado de; OLIVEIRA, Flavia de. Insulin modulates myogenesis and muscle atrophy resulting from skin scald burn in young male rats. Journal Of Surgical Research, [s. l.], v. 257, p. 56-68, jan. 2021. Disponível em: https://www.journalofsurgicalresearch.com/article/S0022-4804(20)30497-2/fulltext. Acesso em: 03 nov. 2020. http://dx.doi.org/10.1016/j.jss.2020.07.040 |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/handle/123456789/31035 |
dc.identifier.issn.none.fl_str_mv |
0022-4804 1095-8673 |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.jss.2020.07.040 |
identifier_str_mv |
QUINTANA, Hananiah Tardivo; BAPTISTA, Vivianne Izabelle de Araújo; LAZZARIN, Mariana Cruz; ANTUNES, Hanna Karen Moreira; LE SUEUR-MALUF, Luciana; OLIVEIRA, Camila Aparecida Machado de; OLIVEIRA, Flavia de. Insulin modulates myogenesis and muscle atrophy resulting from skin scald burn in young male rats. Journal Of Surgical Research, [s. l.], v. 257, p. 56-68, jan. 2021. Disponível em: https://www.journalofsurgicalresearch.com/article/S0022-4804(20)30497-2/fulltext. Acesso em: 03 nov. 2020. http://dx.doi.org/10.1016/j.jss.2020.07.040 0022-4804 1095-8673 10.1016/j.jss.2020.07.040 |
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https://repositorio.ufrn.br/handle/123456789/31035 |
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eng |
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eng |
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Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ |
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openAccess |
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Elsevier |
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Elsevier |
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