The Human Cell Surfaceome of Breast Tumors
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/jspui/handle/123456789/23336 |
Resumo: | Cell surface proteins are ideal targets for cancer therapy and diagnosis. We have identified a set of more than 3700 genes that code for transmembrane proteins believed to be at human cell surface. Methods.We used a high-throuput qPCR system for the analysis of 573 cell surface protein-coding genes in 12 primary breast tumors, 8 breast cell lines, and 21 normal human tissues including breast. To better understand the role of these genes in breast tumors, we used a series of bioinformatics strategies to integrates different type, of the datasets, such as KEGG, protein-protein interaction databases, ONCOMINE, and data from, literature. Results. We found that at least 77 genes are overexpressed in breast primary tumors while at least 2 of them have also a restricted expression pattern in normal tissues. We found common signaling pathways that may be regulated in breast tumors through the overexpression of these cell surface protein-coding genes. Furthermore, a comparison was made between the genes found in this report and other genes associated with features clinically relevant for breast tumorigenesis. Conclusions.The expression profiling generated in this study, togetherwith an integrative bioinformatics analysis, allowed us to identify putative targets for breast tumors. |
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Júlia Pinheiro Chagas da CunhaPedro Alexandre Favoretto GalanteJorge Estefano Santana de SouzaMartin PieprzykDirceMaria CarraroLloyd J. OldAnamaria Aranha CamargoSouza, Sandro José de2017-06-01T12:57:32Z2017-06-01T12:57:32Z20132314-6133https://repositorio.ufrn.br/jspui/handle/123456789/23336engHuman Cell SurfaceomeBreast TumorsThe Human Cell Surfaceome of Breast Tumorsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCell surface proteins are ideal targets for cancer therapy and diagnosis. We have identified a set of more than 3700 genes that code for transmembrane proteins believed to be at human cell surface. Methods.We used a high-throuput qPCR system for the analysis of 573 cell surface protein-coding genes in 12 primary breast tumors, 8 breast cell lines, and 21 normal human tissues including breast. To better understand the role of these genes in breast tumors, we used a series of bioinformatics strategies to integrates different type, of the datasets, such as KEGG, protein-protein interaction databases, ONCOMINE, and data from, literature. Results. We found that at least 77 genes are overexpressed in breast primary tumors while at least 2 of them have also a restricted expression pattern in normal tissues. We found common signaling pathways that may be regulated in breast tumors through the overexpression of these cell surface protein-coding genes. Furthermore, a comparison was made between the genes found in this report and other genes associated with features clinically relevant for breast tumorigenesis. Conclusions.The expression profiling generated in this study, togetherwith an integrative bioinformatics analysis, allowed us to identify putative targets for breast tumors.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALThe Human Cell Surfaceome of Breast Tumors.pdfThe Human Cell Surfaceome of Breast Tumors.pdfArtigo completoapplication/pdf1115789https://repositorio.ufrn.br/bitstream/123456789/23336/1/The%20Human%20Cell%20Surfaceome%20of%20Breast%20Tumors.pdfd68cb2e005408c611093798597b71261MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.ufrn.br/bitstream/123456789/23336/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52TEXTThe Human Cell Surfaceome of Breast Tumors.pdf.txtThe Human Cell Surfaceome of Breast Tumors.pdf.txtExtracted texttext/plain49052https://repositorio.ufrn.br/bitstream/123456789/23336/5/The%20Human%20Cell%20Surfaceome%20of%20Breast%20Tumors.pdf.txt6afdb760228ff2358633f06c2d48b644MD55THUMBNAILThe Human Cell Surfaceome of Breast Tumors.pdf.jpgThe Human Cell Surfaceome of Breast Tumors.pdf.jpgIM Thumbnailimage/jpeg10085https://repositorio.ufrn.br/bitstream/123456789/23336/6/The%20Human%20Cell%20Surfaceome%20of%20Breast%20Tumors.pdf.jpg5f259094de02348eff1c4fc43ffc4bc4MD56123456789/233362021-07-09 19:49:07.217oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-07-09T22:49:07Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
The Human Cell Surfaceome of Breast Tumors |
title |
The Human Cell Surfaceome of Breast Tumors |
spellingShingle |
The Human Cell Surfaceome of Breast Tumors Júlia Pinheiro Chagas da Cunha Human Cell Surfaceome Breast Tumors |
title_short |
The Human Cell Surfaceome of Breast Tumors |
title_full |
The Human Cell Surfaceome of Breast Tumors |
title_fullStr |
The Human Cell Surfaceome of Breast Tumors |
title_full_unstemmed |
The Human Cell Surfaceome of Breast Tumors |
title_sort |
The Human Cell Surfaceome of Breast Tumors |
author |
Júlia Pinheiro Chagas da Cunha |
author_facet |
Júlia Pinheiro Chagas da Cunha Pedro Alexandre Favoretto Galante Jorge Estefano Santana de Souza Martin Pieprzyk DirceMaria Carraro Lloyd J. Old Anamaria Aranha Camargo Souza, Sandro José de |
author_role |
author |
author2 |
Pedro Alexandre Favoretto Galante Jorge Estefano Santana de Souza Martin Pieprzyk DirceMaria Carraro Lloyd J. Old Anamaria Aranha Camargo Souza, Sandro José de |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Júlia Pinheiro Chagas da Cunha Pedro Alexandre Favoretto Galante Jorge Estefano Santana de Souza Martin Pieprzyk DirceMaria Carraro Lloyd J. Old Anamaria Aranha Camargo Souza, Sandro José de |
dc.subject.por.fl_str_mv |
Human Cell Surfaceome Breast Tumors |
topic |
Human Cell Surfaceome Breast Tumors |
description |
Cell surface proteins are ideal targets for cancer therapy and diagnosis. We have identified a set of more than 3700 genes that code for transmembrane proteins believed to be at human cell surface. Methods.We used a high-throuput qPCR system for the analysis of 573 cell surface protein-coding genes in 12 primary breast tumors, 8 breast cell lines, and 21 normal human tissues including breast. To better understand the role of these genes in breast tumors, we used a series of bioinformatics strategies to integrates different type, of the datasets, such as KEGG, protein-protein interaction databases, ONCOMINE, and data from, literature. Results. We found that at least 77 genes are overexpressed in breast primary tumors while at least 2 of them have also a restricted expression pattern in normal tissues. We found common signaling pathways that may be regulated in breast tumors through the overexpression of these cell surface protein-coding genes. Furthermore, a comparison was made between the genes found in this report and other genes associated with features clinically relevant for breast tumorigenesis. Conclusions.The expression profiling generated in this study, togetherwith an integrative bioinformatics analysis, allowed us to identify putative targets for breast tumors. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013 |
dc.date.accessioned.fl_str_mv |
2017-06-01T12:57:32Z |
dc.date.available.fl_str_mv |
2017-06-01T12:57:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/jspui/handle/123456789/23336 |
dc.identifier.issn.none.fl_str_mv |
2314-6133 |
identifier_str_mv |
2314-6133 |
url |
https://repositorio.ufrn.br/jspui/handle/123456789/23336 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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reponame:Repositório Institucional da UFRN instname:Universidade Federal do Rio Grande do Norte (UFRN) instacron:UFRN |
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Universidade Federal do Rio Grande do Norte (UFRN) |
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UFRN |
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UFRN |
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Repositório Institucional da UFRN |
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Repositório Institucional da UFRN |
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