Análise de células T regulatórias FoxP3+ no líquen plano oral
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/jspui/handle/123456789/17110 |
Resumo: | T regulatory cells have the function of controlling immune responses and maintaining self-tolerance. The FoxP3 has been considered the most specific marker for Treg cells. The aiming of this paper was to evaluate the immunoexpression of FoxP3 in the inflammatory infiltrate from oral lichen planus (OLP) and to compare it with the infiltrate in fibrous inflammatory hyperplasia (FIH) and then, between reticular and erosive forms of OLP. The samples were composed by 32 cases of OLP (17 reticular and 15 erosive) beyond 10 cases of FIH that were submitted to immunohistochemistry staining for FoxP3. Localization of the staining was classified in underepithelial and intraepithelial and the amount of FoxP3+ cells was evaluated through cells counting in 10 consecutive fields, at 400x power magnification. The values were expressed in mean ± standart deviation, and submitted to statistical tests with 5% of significance level. It was observed a statistical significant difference in the amount of FoxP3+ Treg cells between the two combined forms of OLP (1,6 ± 2,2) and the FIH (0,5 ±0,4) (P<0,05). This maybe could be explained by immunological mechanism of OLP, which involves a permanent antigenic induction likely with consequent perpetuation of lesion, eliciting the proliferation and constant recruitment of Treg cells. Otherwise, FIH presents a different etiopathogenesis, in which there is also generation of a variable inflammatory infiltrate, however qualitatively distinct from that seen in OLP. The erosive form of OLP exhibited a greater number (1,7 ± 2,4) of FoxP3+ Treg cells than reticular form (1,5 ± 2,1). These alterations could have relation with the great disease activity verified in erosive OLP, or also, with abnormalities in the regulatory function of Treg cells that could cause the increase observed. Considering the capacity already well established in the literature, both about Treg cells in modulating immune responses, as in the oral mucosa in showing great potential for regeneration, it is suggested that the possibility of development and implantation of immunotherapeutic strategies that regulate the frequency and function of these cells, may help in future treatment of immune-mediated inflammatory diseases such as OLP |
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Pereira, Joabe dos Santoshttp://lattes.cnpq.br/6228325319211685http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707501Z3&dataRevisao=nullGodoy, Gustavo Pinahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4751939A2Souza, Lélia Batista dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787927Z7&dataRevisao=nullMiguel, Márcia Cristina da Costa2014-12-17T15:32:18Z2010-05-032014-12-17T15:32:18Z2010-03-15PEREIRA, Joabe dos Santos. Análise de células T regulatórias FoxP3+ no líquen plano oral. 2010. 109 f. Dissertação (Mestrado em Odontologia) - Universidade Federal do Rio Grande do Norte, Natal, 2010.https://repositorio.ufrn.br/jspui/handle/123456789/17110T regulatory cells have the function of controlling immune responses and maintaining self-tolerance. The FoxP3 has been considered the most specific marker for Treg cells. The aiming of this paper was to evaluate the immunoexpression of FoxP3 in the inflammatory infiltrate from oral lichen planus (OLP) and to compare it with the infiltrate in fibrous inflammatory hyperplasia (FIH) and then, between reticular and erosive forms of OLP. The samples were composed by 32 cases of OLP (17 reticular and 15 erosive) beyond 10 cases of FIH that were submitted to immunohistochemistry staining for FoxP3. Localization of the staining was classified in underepithelial and intraepithelial and the amount of FoxP3+ cells was evaluated through cells counting in 10 consecutive fields, at 400x power magnification. The values were expressed in mean ± standart deviation, and submitted to statistical tests with 5% of significance level. It was observed a statistical significant difference in the amount of FoxP3+ Treg cells between the two combined forms of OLP (1,6 ± 2,2) and the FIH (0,5 ±0,4) (P<0,05). This maybe could be explained by immunological mechanism of OLP, which involves a permanent antigenic induction likely with consequent perpetuation of lesion, eliciting the proliferation and constant recruitment of Treg cells. Otherwise, FIH presents a different etiopathogenesis, in which there is also generation of a variable inflammatory infiltrate, however qualitatively distinct from that seen in OLP. The erosive form of OLP exhibited a greater number (1,7 ± 2,4) of FoxP3+ Treg cells than reticular form (1,5 ± 2,1). These alterations could have relation with the great disease activity verified in erosive OLP, or also, with abnormalities in the regulatory function of Treg cells that could cause the increase observed. Considering the capacity already well established in the literature, both about Treg cells in modulating immune responses, as in the oral mucosa in showing great potential for regeneration, it is suggested that the possibility of development and implantation of immunotherapeutic strategies that regulate the frequency and function of these cells, may help in future treatment of immune-mediated inflammatory diseases such as OLPAs células T regulatórias (Treg) possuem a função de controlar respostas imunes e manter a autotolerância. O FoxP3 tem sido considerado o marcador mais específico para células Treg. O objetivo deste estudo foi avaliar a imunoexpressão do FoxP3 no infiltrado inflamatório do líquen plano oral (LPO) comparado ao da hiperplasia fibrosa inflamatória (HFI) e posteriormente entre as formas reticular e erosiva do LPO. A amostra foi composta por 32 casos de LPO (17 reticulares e 15 erosivos) além de 10 casos de HFI que foram submetidos à marcação imunoistoquímica para o FoxP3. A localização da marcação foi classificada em justaepitelial ou intraepitelial e a quantidade das células FoxP3+ foi avaliada através da contagem destas em 10 campos consecutivos, com aumento de 400x. Os valores foram expressos em média ± desvio-padrão, e submetidos aos testes estatísticos com nível de significância de 5%. Observou-se uma diferença estatisticamente significativa na quantidade de células Treg FoxP3+ entre os dois tipos de LPO reunidos (1,6 ± 2,2) e a HFI (0,5 ± 0,4) (P<0,05). Isto talvez possa ser explicado pelo mecanismo imunológico do LPO, que envolve uma provável indução antigênica permanente com conseqüente perpetuação da lesão, suscitando a proliferação e recrutamento constante das células Treg. Em contrapartida, a HFI apresenta uma etiopatogenia diferente, na qual também há geração de um infiltrado inflamatório variável, porém qualitativamente distinto do verificado no LPO. A forma erosiva do LPO exibiu um maior número (1,7 ± 2,4) de células Treg FoxP3+ que a forma reticular (1,5 ± 2,1). Estas alterações podem ter relação com a maior atividade da doença verificada no LPO erosivo, ou ainda, com anormalidades na função reguladora das células Treg que ocasionariam o aumento observado. Considerando-se a capacidade já bem estabelecida na literatura, tanto das células Treg modularem as respostas imunológicas, quanto da mucosa oral em exibir um grande potencial de regeneração, sugere-se que a possibilidade de desenvolvimento e implantação de estratégias imunoterapêuticas que regulem a freqüência e a função destas células, possa futuramente auxiliar no tratamento de doenças inflamatórias mediadas imunologicamente, como o LPOCoordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal do Rio Grande do NortePrograma de Pós-Graduação em Patologia OralUFRNBROdontologiaLíquen plano oralImunoistoquímicaCélula T regulatóriaFoxP3Oral lichen planusImmunohistochemistryRegulatory T cellsFoxP3CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIAAnálise de células T regulatórias FoxP3+ no líquen plano oralinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALJoabeSP.pdfapplication/pdf1318134https://repositorio.ufrn.br/bitstream/123456789/17110/1/JoabeSP.pdf051d3ab3425024319d41e93c3e0af738MD51TEXTJoabeSP.pdf.txtJoabeSP.pdf.txtExtracted texttext/plain170343https://repositorio.ufrn.br/bitstream/123456789/17110/6/JoabeSP.pdf.txt56b2db4b12122a8eb78d122527a6d14fMD56THUMBNAILJoabeSP.pdf.jpgJoabeSP.pdf.jpgIM Thumbnailimage/jpeg3272https://repositorio.ufrn.br/bitstream/123456789/17110/7/JoabeSP.pdf.jpg2a25078a356ee5da1f4ad4818bd0aa68MD57123456789/171102017-11-04 13:14:35.833oai:https://repositorio.ufrn.br:123456789/17110Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2017-11-04T16:14:35Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.por.fl_str_mv |
Análise de células T regulatórias FoxP3+ no líquen plano oral |
title |
Análise de células T regulatórias FoxP3+ no líquen plano oral |
spellingShingle |
Análise de células T regulatórias FoxP3+ no líquen plano oral Pereira, Joabe dos Santos Líquen plano oral Imunoistoquímica Célula T regulatória FoxP3 Oral lichen planus Immunohistochemistry Regulatory T cells FoxP3 CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
title_short |
Análise de células T regulatórias FoxP3+ no líquen plano oral |
title_full |
Análise de células T regulatórias FoxP3+ no líquen plano oral |
title_fullStr |
Análise de células T regulatórias FoxP3+ no líquen plano oral |
title_full_unstemmed |
Análise de células T regulatórias FoxP3+ no líquen plano oral |
title_sort |
Análise de células T regulatórias FoxP3+ no líquen plano oral |
author |
Pereira, Joabe dos Santos |
author_facet |
Pereira, Joabe dos Santos |
author_role |
author |
dc.contributor.authorID.por.fl_str_mv |
|
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/6228325319211685 |
dc.contributor.advisorID.por.fl_str_mv |
|
dc.contributor.advisorLattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707501Z3&dataRevisao=null |
dc.contributor.referees1.pt_BR.fl_str_mv |
Godoy, Gustavo Pina |
dc.contributor.referees1ID.por.fl_str_mv |
|
dc.contributor.referees1Lattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4751939A2 |
dc.contributor.referees2.pt_BR.fl_str_mv |
Souza, Lélia Batista de |
dc.contributor.referees2Lattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787927Z7&dataRevisao=null |
dc.contributor.author.fl_str_mv |
Pereira, Joabe dos Santos |
dc.contributor.advisor1.fl_str_mv |
Miguel, Márcia Cristina da Costa |
contributor_str_mv |
Miguel, Márcia Cristina da Costa |
dc.subject.por.fl_str_mv |
Líquen plano oral Imunoistoquímica Célula T regulatória FoxP3 |
topic |
Líquen plano oral Imunoistoquímica Célula T regulatória FoxP3 Oral lichen planus Immunohistochemistry Regulatory T cells FoxP3 CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
dc.subject.eng.fl_str_mv |
Oral lichen planus Immunohistochemistry Regulatory T cells FoxP3 |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
description |
T regulatory cells have the function of controlling immune responses and maintaining self-tolerance. The FoxP3 has been considered the most specific marker for Treg cells. The aiming of this paper was to evaluate the immunoexpression of FoxP3 in the inflammatory infiltrate from oral lichen planus (OLP) and to compare it with the infiltrate in fibrous inflammatory hyperplasia (FIH) and then, between reticular and erosive forms of OLP. The samples were composed by 32 cases of OLP (17 reticular and 15 erosive) beyond 10 cases of FIH that were submitted to immunohistochemistry staining for FoxP3. Localization of the staining was classified in underepithelial and intraepithelial and the amount of FoxP3+ cells was evaluated through cells counting in 10 consecutive fields, at 400x power magnification. The values were expressed in mean ± standart deviation, and submitted to statistical tests with 5% of significance level. It was observed a statistical significant difference in the amount of FoxP3+ Treg cells between the two combined forms of OLP (1,6 ± 2,2) and the FIH (0,5 ±0,4) (P<0,05). This maybe could be explained by immunological mechanism of OLP, which involves a permanent antigenic induction likely with consequent perpetuation of lesion, eliciting the proliferation and constant recruitment of Treg cells. Otherwise, FIH presents a different etiopathogenesis, in which there is also generation of a variable inflammatory infiltrate, however qualitatively distinct from that seen in OLP. The erosive form of OLP exhibited a greater number (1,7 ± 2,4) of FoxP3+ Treg cells than reticular form (1,5 ± 2,1). These alterations could have relation with the great disease activity verified in erosive OLP, or also, with abnormalities in the regulatory function of Treg cells that could cause the increase observed. Considering the capacity already well established in the literature, both about Treg cells in modulating immune responses, as in the oral mucosa in showing great potential for regeneration, it is suggested that the possibility of development and implantation of immunotherapeutic strategies that regulate the frequency and function of these cells, may help in future treatment of immune-mediated inflammatory diseases such as OLP |
publishDate |
2010 |
dc.date.available.fl_str_mv |
2010-05-03 2014-12-17T15:32:18Z |
dc.date.issued.fl_str_mv |
2010-03-15 |
dc.date.accessioned.fl_str_mv |
2014-12-17T15:32:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PEREIRA, Joabe dos Santos. Análise de células T regulatórias FoxP3+ no líquen plano oral. 2010. 109 f. Dissertação (Mestrado em Odontologia) - Universidade Federal do Rio Grande do Norte, Natal, 2010. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/jspui/handle/123456789/17110 |
identifier_str_mv |
PEREIRA, Joabe dos Santos. Análise de células T regulatórias FoxP3+ no líquen plano oral. 2010. 109 f. Dissertação (Mestrado em Odontologia) - Universidade Federal do Rio Grande do Norte, Natal, 2010. |
url |
https://repositorio.ufrn.br/jspui/handle/123456789/17110 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal do Rio Grande do Norte |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Patologia Oral |
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UFRN |
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BR |
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Odontologia |
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Universidade Federal do Rio Grande do Norte |
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