Behavioral changes over time following ayahuasca exposure in zebrafish
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/jspui/handle/123456789/24536 |
Resumo: | The combined infusion of Banisteriopsis caapi stem and Psychotria viridis leaves, known as ayahuasca, has been used for centuries by indigenous tribes. The infusion is rich in N, N-dimethyltryptamine (DMT) and monoamine oxidase inhibitors, with properties similar to those of serotonin. Despite substantial progress in the development of new drugs to treat anxiety and depression, current treatments have several limitations. Alternative drugs, such as ayahuasca, may shed light on these disorders. Here, we present time-course behavioral changes induced by ayahuasca in zebrafish, as first step toward establishing an ideal concentration for pre-clinical evaluations. We exposed adult zebrafish to five concentrations of the ayahuasca infusion: 0 (control), 0.1, 0.5, 1, and 3 ml/L (n D 14 each group), and behavior was recorded for 60 min. We evaluated swimming speed, distance traveled, freezing and bottom dwelling every min for 60 min. Swimming speed and distance traveled decreased with an increase in ayahuasca concentration while freezing increased with 1 and 3 ml/L. Bottom dwelling increased with 1 and 3 ml/L, but declined with 0.1 ml/L. Our data suggest that small amounts of ayahuasca do not affect locomotion and reduce anxiety-like behavior in zebrafish, while increased doses of the drug lead to crescent anxiogenic effects. We conclude that the temporal analysis of zebrafish behavior is a sensitive method for the study of ayahuasca-induced functional changes in the vertebrate brain |
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Savoldi, RobsonPolari, DanielSilva, Jaquelinne Pinheiro daSilva, PriscilaSoares, Bruno LobaoYonamine, MauricioFreire, Fúlvio Aurélio de MoraisLuchiari, Ana Carolina2018-01-08T14:48:20Z2018-01-08T14:48:20Z2017-07-28SAVOLDI, Robson; et al. Behavioral changes over time following ayahuasca exposure in zebrafish. Frontiers in Behavioral Neuroscience, v. 11, p. 139, jul. 2017. Disponível em <http://journal.frontiersin.org/article/10.3389/fnbeh.2017.00139/full>. Acesso em: 16 out. 2017.https://repositorio.ufrn.br/jspui/handle/123456789/2453610.3389/fnbeh.2017.00139engEntheogenPsychoactiveDrugMedicineDepressionBehavioral changes over time following ayahuasca exposure in zebrafishinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleThe combined infusion of Banisteriopsis caapi stem and Psychotria viridis leaves, known as ayahuasca, has been used for centuries by indigenous tribes. The infusion is rich in N, N-dimethyltryptamine (DMT) and monoamine oxidase inhibitors, with properties similar to those of serotonin. Despite substantial progress in the development of new drugs to treat anxiety and depression, current treatments have several limitations. Alternative drugs, such as ayahuasca, may shed light on these disorders. Here, we present time-course behavioral changes induced by ayahuasca in zebrafish, as first step toward establishing an ideal concentration for pre-clinical evaluations. We exposed adult zebrafish to five concentrations of the ayahuasca infusion: 0 (control), 0.1, 0.5, 1, and 3 ml/L (n D 14 each group), and behavior was recorded for 60 min. We evaluated swimming speed, distance traveled, freezing and bottom dwelling every min for 60 min. Swimming speed and distance traveled decreased with an increase in ayahuasca concentration while freezing increased with 1 and 3 ml/L. Bottom dwelling increased with 1 and 3 ml/L, but declined with 0.1 ml/L. Our data suggest that small amounts of ayahuasca do not affect locomotion and reduce anxiety-like behavior in zebrafish, while increased doses of the drug lead to crescent anxiogenic effects. We conclude that the temporal analysis of zebrafish behavior is a sensitive method for the study of ayahuasca-induced functional changes in the vertebrate braininfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALBehavioralChangesOver_Savoldi_2017.pdfBehavioralChangesOver_Savoldi_2017.pdfapplication/pdf768256https://repositorio.ufrn.br/bitstream/123456789/24536/1/BehavioralChangesOver_Savoldi_2017.pdf1b320264a50b2e0e24942b4441f2bbb9MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.ufrn.br/bitstream/123456789/24536/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52TEXTBehavioralChangesOver_2017.pdf.txtBehavioralChangesOver_2017.pdf.txtExtracted texttext/plain55583https://repositorio.ufrn.br/bitstream/123456789/24536/3/BehavioralChangesOver_2017.pdf.txtef6b1ac6903148c4eae1c5f1dd769c49MD53THUMBNAILBehavioralChangesOver_2017.pdf.jpgBehavioralChangesOver_2017.pdf.jpgIM Thumbnailimage/jpeg10984https://repositorio.ufrn.br/bitstream/123456789/24536/4/BehavioralChangesOver_2017.pdf.jpg69f05aaf87f3832a383888f048699001MD54123456789/245362021-11-08 17:15:24.694oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-11-08T20:15:24Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Behavioral changes over time following ayahuasca exposure in zebrafish |
title |
Behavioral changes over time following ayahuasca exposure in zebrafish |
spellingShingle |
Behavioral changes over time following ayahuasca exposure in zebrafish Savoldi, Robson Entheogen Psychoactive Drug Medicine Depression |
title_short |
Behavioral changes over time following ayahuasca exposure in zebrafish |
title_full |
Behavioral changes over time following ayahuasca exposure in zebrafish |
title_fullStr |
Behavioral changes over time following ayahuasca exposure in zebrafish |
title_full_unstemmed |
Behavioral changes over time following ayahuasca exposure in zebrafish |
title_sort |
Behavioral changes over time following ayahuasca exposure in zebrafish |
author |
Savoldi, Robson |
author_facet |
Savoldi, Robson Polari, Daniel Silva, Jaquelinne Pinheiro da Silva, Priscila Soares, Bruno Lobao Yonamine, Mauricio Freire, Fúlvio Aurélio de Morais Luchiari, Ana Carolina |
author_role |
author |
author2 |
Polari, Daniel Silva, Jaquelinne Pinheiro da Silva, Priscila Soares, Bruno Lobao Yonamine, Mauricio Freire, Fúlvio Aurélio de Morais Luchiari, Ana Carolina |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Savoldi, Robson Polari, Daniel Silva, Jaquelinne Pinheiro da Silva, Priscila Soares, Bruno Lobao Yonamine, Mauricio Freire, Fúlvio Aurélio de Morais Luchiari, Ana Carolina |
dc.subject.por.fl_str_mv |
Entheogen Psychoactive Drug Medicine Depression |
topic |
Entheogen Psychoactive Drug Medicine Depression |
description |
The combined infusion of Banisteriopsis caapi stem and Psychotria viridis leaves, known as ayahuasca, has been used for centuries by indigenous tribes. The infusion is rich in N, N-dimethyltryptamine (DMT) and monoamine oxidase inhibitors, with properties similar to those of serotonin. Despite substantial progress in the development of new drugs to treat anxiety and depression, current treatments have several limitations. Alternative drugs, such as ayahuasca, may shed light on these disorders. Here, we present time-course behavioral changes induced by ayahuasca in zebrafish, as first step toward establishing an ideal concentration for pre-clinical evaluations. We exposed adult zebrafish to five concentrations of the ayahuasca infusion: 0 (control), 0.1, 0.5, 1, and 3 ml/L (n D 14 each group), and behavior was recorded for 60 min. We evaluated swimming speed, distance traveled, freezing and bottom dwelling every min for 60 min. Swimming speed and distance traveled decreased with an increase in ayahuasca concentration while freezing increased with 1 and 3 ml/L. Bottom dwelling increased with 1 and 3 ml/L, but declined with 0.1 ml/L. Our data suggest that small amounts of ayahuasca do not affect locomotion and reduce anxiety-like behavior in zebrafish, while increased doses of the drug lead to crescent anxiogenic effects. We conclude that the temporal analysis of zebrafish behavior is a sensitive method for the study of ayahuasca-induced functional changes in the vertebrate brain |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-07-28 |
dc.date.accessioned.fl_str_mv |
2018-01-08T14:48:20Z |
dc.date.available.fl_str_mv |
2018-01-08T14:48:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SAVOLDI, Robson; et al. Behavioral changes over time following ayahuasca exposure in zebrafish. Frontiers in Behavioral Neuroscience, v. 11, p. 139, jul. 2017. Disponível em <http://journal.frontiersin.org/article/10.3389/fnbeh.2017.00139/full>. Acesso em: 16 out. 2017. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/jspui/handle/123456789/24536 |
dc.identifier.doi.none.fl_str_mv |
10.3389/fnbeh.2017.00139 |
identifier_str_mv |
SAVOLDI, Robson; et al. Behavioral changes over time following ayahuasca exposure in zebrafish. Frontiers in Behavioral Neuroscience, v. 11, p. 139, jul. 2017. Disponível em <http://journal.frontiersin.org/article/10.3389/fnbeh.2017.00139/full>. Acesso em: 16 out. 2017. 10.3389/fnbeh.2017.00139 |
url |
https://repositorio.ufrn.br/jspui/handle/123456789/24536 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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