Extração de compostos fenólicos do bagaço do caju (Anacardium occidentale L.) visando aplicação cosmética
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Trabalho de conclusão de curso |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/handle/123456789/55942 |
Resumo: | The cashew apple bagasse (CAB) is an agroindustrial byproduct composed of lignocellulose and serves as a significant source of phenolic compounds. In this context, the aim was to obtain a phenolic-rich extract using CAB for cosmetic applications. CAB underwent pretreatments with liquid hot water (LHW) and steam explosion (SE), characterized by FTIR, XRD, and lignocellulosic composition. Subsequently, enzymatic-assisted extraction (EAE) of pre-treated CAB was assessed using a cellulolytic cocktail. The effects of enzyme load, solid load, and incubation time were optimized through a 23 central composite rotational design. Extracts were evaluated for phenolic composition, in vitro and cellular antioxidant activity, sugar content, in vitro sun protection factor (SPF), and cytotoxicity. Results demonstrated structural modifications in the residue through the removal of amorphous fractions. The HW pretreatment combined with enzymatic extraction facilitated phenolic release (75.13 ± 3.5 mg GAE/L) and enhanced antioxidant activity (72.81% for DPPH and 241.04 ± 1.7 µM EQ Trolox/L for ABTS). Furthermore, the extract was non-toxic, with cellular viability exceeding 95% in MRC-5. Statistical design indicated optimal conditions of 80 h incubation and 8% solid load. The cashew extract exhibited cellular antioxidant activity (36.03% inhibition of Reactive Oxygen Species) and indications of solar photoprotection (SPF 6.4). Therefore, obtaining a cashew extract proves promising for anti-aging cosmetic formulations. |
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Alves, Fábia Rafaella Silva AlvesSena, Ana Beatriz da Silva AraújoPadilha, Carlos Eduardo de AraújoAssis, Cristiane Fernandes deSilva, Bárbara Janaína Paula daLima, Emersom SilvaJúnior, Francisco Canindé de SousaConverti, AttilioLima, Ádley Antonini Neves dehttps://orcid.org/0000-0001-7142-4626http://lattes.cnpq.br/3654181559500206https://orcid.org/0000-0002-3798-3915http://lattes.cnpq.br/4061809277935577Alves, Fábia Rafaella Silvahttps://orcid.org/0000-0002-6634-7125http://lattes.cnpq.br/4939875215993291Lima, Ádley Antonini Neves dehttps://orcid.org/0000-0002-3798-3915http://lattes.cnpq.br/4061809277935577Assis, Cristiane Fernandes dehttps://orcid.org/0000-0001-7595-5395http://lattes.cnpq.br/0034694007210837Oliveira, Verônica da Silvahttp://lattes.cnpq.br/3459683395706868Lima, Ádley Antonini Neves de2023-12-14T20:28:44Z2023-12-14T20:28:44Z2023-12-01SENA, Ana Beatriz da Silva Araújo. Extração de compostos fenólicos do bagaço do caju (Anacardium occidentale L.) visando aplicação cosmética. Orientador: Ádley Antonini Neves de Lima. 2023. 51 f. Trabalho de Conclusão de Curso (Graduação em Farmácia) - Departamento de Farmácia, Universidade Federal do Rio Grande do Norte, Natal, 2023.https://repositorio.ufrn.br/handle/123456789/55942The cashew apple bagasse (CAB) is an agroindustrial byproduct composed of lignocellulose and serves as a significant source of phenolic compounds. In this context, the aim was to obtain a phenolic-rich extract using CAB for cosmetic applications. CAB underwent pretreatments with liquid hot water (LHW) and steam explosion (SE), characterized by FTIR, XRD, and lignocellulosic composition. Subsequently, enzymatic-assisted extraction (EAE) of pre-treated CAB was assessed using a cellulolytic cocktail. The effects of enzyme load, solid load, and incubation time were optimized through a 23 central composite rotational design. Extracts were evaluated for phenolic composition, in vitro and cellular antioxidant activity, sugar content, in vitro sun protection factor (SPF), and cytotoxicity. Results demonstrated structural modifications in the residue through the removal of amorphous fractions. The HW pretreatment combined with enzymatic extraction facilitated phenolic release (75.13 ± 3.5 mg GAE/L) and enhanced antioxidant activity (72.81% for DPPH and 241.04 ± 1.7 µM EQ Trolox/L for ABTS). Furthermore, the extract was non-toxic, with cellular viability exceeding 95% in MRC-5. Statistical design indicated optimal conditions of 80 h incubation and 8% solid load. The cashew extract exhibited cellular antioxidant activity (36.03% inhibition of Reactive Oxygen Species) and indications of solar photoprotection (SPF 6.4). Therefore, obtaining a cashew extract proves promising for anti-aging cosmetic formulations.The cashew apple bagasse (CAB) is an agroindustrial byproduct composed of lignocellulose and serves as a significant source of phenolic compounds. In this context, the aim was to obtain a phenolic-rich extract using CAB for cosmetic applications. CAB underwent pretreatments with liquid hot water (LHW) and steam explosion (SE), characterized by FTIR, XRD, and lignocellulosic composition. Subsequently, enzymatic-assisted extraction (EAE) of pre-treated CAB was assessed using a cellulolytic cocktail. The effects of enzyme load, solid load, and incubation time were optimized through a 23 central composite rotational design. Extracts were evaluated for phenolic composition, in vitro and cellular antioxidant activity, sugar content, in vitro sun protection factor (SPF), and cytotoxicity. Results demonstrated structural modifications in the residue through the removal of amorphous fractions. The HW pretreatment combined with enzymatic extraction facilitated phenolic release (75.13 ± 3.5 mg GAE/L) and enhanced antioxidant activity (72.81% for DPPH and 241.04 ± 1.7 µM EQ Trolox/L for ABTS). Furthermore, the extract was non-toxic, with cellular viability exceeding 95% in MRC-5. Statistical design indicated optimal conditions of 80 h incubation and 8% solid load. The cashew extract exhibited cellular antioxidant activity (36.03% inhibition of Reactive Oxygen Species) and indications of solar photoprotection (SPF 6.4). Therefore, obtaining a cashew extract proves promising for anti-aging cosmetic formulations.CNPQ e CAPESUniversidade Federal do Rio Grande do NorteFarmáciaUFRNBrasilDEPARTAMENTO DE FARMÁCIAAttribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessCNPQ::CIENCIAS DA SAUDECashew apple bagasseEnzyme-assisted extractionPhenolic compoundsAntioxidant activityCosmeticBagaço de cajuExtração assistida por enzimaCompostos fenólicosAtividade antioxidanteCosméticoExtração de compostos fenólicos do bagaço do caju (Anacardium occidentale L.) visando aplicação cosméticaExtraction of phenolic compounds from cashew apple bagasse (Anacardium occidentale L.) aiming at cosmetic applicationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALTCC BEATRIZ - ultima versão.pdfTCC BEATRIZ - ultima versão.pdfArquivo PDF do TCCapplication/pdf1030614https://repositorio.ufrn.br/bitstream/123456789/55942/1/TCC%20BEATRIZ%20%20-%20ultima%20vers%c3%a3o.pdf4f2c8ff41111a648b0c6ab0ec0021eb5MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.ufrn.br/bitstream/123456789/55942/2/license_rdfe39d27027a6cc9cb039ad269a5db8e34MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/55942/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53123456789/559422023-12-14 17:28:44.698oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2023-12-14T20:28:44Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Extração de compostos fenólicos do bagaço do caju (Anacardium occidentale L.) visando aplicação cosmética |
dc.title.alternative.pt_BR.fl_str_mv |
Extraction of phenolic compounds from cashew apple bagasse (Anacardium occidentale L.) aiming at cosmetic application |
title |
Extração de compostos fenólicos do bagaço do caju (Anacardium occidentale L.) visando aplicação cosmética |
spellingShingle |
Extração de compostos fenólicos do bagaço do caju (Anacardium occidentale L.) visando aplicação cosmética Alves, Fábia Rafaella Silva Alves CNPQ::CIENCIAS DA SAUDE Cashew apple bagasse Enzyme-assisted extraction Phenolic compounds Antioxidant activity Cosmetic Bagaço de caju Extração assistida por enzima Compostos fenólicos Atividade antioxidante Cosmético |
title_short |
Extração de compostos fenólicos do bagaço do caju (Anacardium occidentale L.) visando aplicação cosmética |
title_full |
Extração de compostos fenólicos do bagaço do caju (Anacardium occidentale L.) visando aplicação cosmética |
title_fullStr |
Extração de compostos fenólicos do bagaço do caju (Anacardium occidentale L.) visando aplicação cosmética |
title_full_unstemmed |
Extração de compostos fenólicos do bagaço do caju (Anacardium occidentale L.) visando aplicação cosmética |
title_sort |
Extração de compostos fenólicos do bagaço do caju (Anacardium occidentale L.) visando aplicação cosmética |
author |
Alves, Fábia Rafaella Silva Alves |
author_facet |
Alves, Fábia Rafaella Silva Alves Sena, Ana Beatriz da Silva Araújo Padilha, Carlos Eduardo de Araújo Assis, Cristiane Fernandes de Silva, Bárbara Janaína Paula da Lima, Emersom Silva Júnior, Francisco Canindé de Sousa Converti, Attilio Lima, Ádley Antonini Neves de |
author_role |
author |
author2 |
Sena, Ana Beatriz da Silva Araújo Padilha, Carlos Eduardo de Araújo Assis, Cristiane Fernandes de Silva, Bárbara Janaína Paula da Lima, Emersom Silva Júnior, Francisco Canindé de Sousa Converti, Attilio Lima, Ádley Antonini Neves de |
author2_role |
author author author author author author author author |
dc.contributor.authorID.pt_BR.fl_str_mv |
https://orcid.org/0000-0001-7142-4626 |
dc.contributor.authorLattes.pt_BR.fl_str_mv |
http://lattes.cnpq.br/3654181559500206 |
dc.contributor.advisorID.pt_BR.fl_str_mv |
https://orcid.org/0000-0002-3798-3915 |
dc.contributor.advisorLattes.pt_BR.fl_str_mv |
http://lattes.cnpq.br/4061809277935577 |
dc.contributor.referees1.none.fl_str_mv |
Lima, Ádley Antonini Neves de |
dc.contributor.referees1ID.pt_BR.fl_str_mv |
https://orcid.org/0000-0002-3798-3915 |
dc.contributor.referees1Lattes.pt_BR.fl_str_mv |
http://lattes.cnpq.br/4061809277935577 |
dc.contributor.referees2.none.fl_str_mv |
Assis, Cristiane Fernandes de |
dc.contributor.referees2ID.pt_BR.fl_str_mv |
https://orcid.org/0000-0001-7595-5395 |
dc.contributor.referees2Lattes.pt_BR.fl_str_mv |
http://lattes.cnpq.br/0034694007210837 |
dc.contributor.referees3.none.fl_str_mv |
Oliveira, Verônica da Silva |
dc.contributor.referees3Lattes.pt_BR.fl_str_mv |
http://lattes.cnpq.br/3459683395706868 |
dc.contributor.author.fl_str_mv |
Alves, Fábia Rafaella Silva Alves Sena, Ana Beatriz da Silva Araújo Padilha, Carlos Eduardo de Araújo Assis, Cristiane Fernandes de Silva, Bárbara Janaína Paula da Lima, Emersom Silva Júnior, Francisco Canindé de Sousa Converti, Attilio Lima, Ádley Antonini Neves de |
dc.contributor.advisor-co1.fl_str_mv |
Alves, Fábia Rafaella Silva |
dc.contributor.advisor-co1ID.fl_str_mv |
https://orcid.org/0000-0002-6634-7125 |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/4939875215993291 |
dc.contributor.advisor1.fl_str_mv |
Lima, Ádley Antonini Neves de |
contributor_str_mv |
Alves, Fábia Rafaella Silva Lima, Ádley Antonini Neves de |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE |
topic |
CNPQ::CIENCIAS DA SAUDE Cashew apple bagasse Enzyme-assisted extraction Phenolic compounds Antioxidant activity Cosmetic Bagaço de caju Extração assistida por enzima Compostos fenólicos Atividade antioxidante Cosmético |
dc.subject.por.fl_str_mv |
Cashew apple bagasse Enzyme-assisted extraction Phenolic compounds Antioxidant activity Cosmetic Bagaço de caju Extração assistida por enzima Compostos fenólicos Atividade antioxidante Cosmético |
description |
The cashew apple bagasse (CAB) is an agroindustrial byproduct composed of lignocellulose and serves as a significant source of phenolic compounds. In this context, the aim was to obtain a phenolic-rich extract using CAB for cosmetic applications. CAB underwent pretreatments with liquid hot water (LHW) and steam explosion (SE), characterized by FTIR, XRD, and lignocellulosic composition. Subsequently, enzymatic-assisted extraction (EAE) of pre-treated CAB was assessed using a cellulolytic cocktail. The effects of enzyme load, solid load, and incubation time were optimized through a 23 central composite rotational design. Extracts were evaluated for phenolic composition, in vitro and cellular antioxidant activity, sugar content, in vitro sun protection factor (SPF), and cytotoxicity. Results demonstrated structural modifications in the residue through the removal of amorphous fractions. The HW pretreatment combined with enzymatic extraction facilitated phenolic release (75.13 ± 3.5 mg GAE/L) and enhanced antioxidant activity (72.81% for DPPH and 241.04 ± 1.7 µM EQ Trolox/L for ABTS). Furthermore, the extract was non-toxic, with cellular viability exceeding 95% in MRC-5. Statistical design indicated optimal conditions of 80 h incubation and 8% solid load. The cashew extract exhibited cellular antioxidant activity (36.03% inhibition of Reactive Oxygen Species) and indications of solar photoprotection (SPF 6.4). Therefore, obtaining a cashew extract proves promising for anti-aging cosmetic formulations. |
publishDate |
2023 |
dc.date.accessioned.fl_str_mv |
2023-12-14T20:28:44Z |
dc.date.available.fl_str_mv |
2023-12-14T20:28:44Z |
dc.date.issued.fl_str_mv |
2023-12-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/bachelorThesis |
format |
bachelorThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SENA, Ana Beatriz da Silva Araújo. Extração de compostos fenólicos do bagaço do caju (Anacardium occidentale L.) visando aplicação cosmética. Orientador: Ádley Antonini Neves de Lima. 2023. 51 f. Trabalho de Conclusão de Curso (Graduação em Farmácia) - Departamento de Farmácia, Universidade Federal do Rio Grande do Norte, Natal, 2023. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/handle/123456789/55942 |
identifier_str_mv |
SENA, Ana Beatriz da Silva Araújo. Extração de compostos fenólicos do bagaço do caju (Anacardium occidentale L.) visando aplicação cosmética. Orientador: Ádley Antonini Neves de Lima. 2023. 51 f. Trabalho de Conclusão de Curso (Graduação em Farmácia) - Departamento de Farmácia, Universidade Federal do Rio Grande do Norte, Natal, 2023. |
url |
https://repositorio.ufrn.br/handle/123456789/55942 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal do Rio Grande do Norte |
dc.publisher.program.fl_str_mv |
Farmácia |
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UFRN |
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Brasil |
dc.publisher.department.fl_str_mv |
DEPARTAMENTO DE FARMÁCIA |
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Universidade Federal do Rio Grande do Norte |
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