Effects of photodynamic therapy mediated by nanoemulsion containing chloro-aluminum phthalocyanine: a histologic and immunohistochemical study in human gingiva
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Publication Date: | 2015 |
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Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UFRN |
Download full: | https://repositorio.ufrn.br/jspui/handle/123456789/22995 http://doi.org/10.1016/j.pdpdt.2015.10.009 |
Summary: | Background Photodynamic therapy (PDT) uses photosensitizing agents, which are delivered in target cells, followed by local application of visible light in specific wavelengths. This reaction produce reactive oxygen species able to induce cell death by apoptosis or necrosis, injured to the local vasculature, and exert important effects on the immune system. Objective The present work evaluated the clinical findings, histomorphological alterations and immunodetection of VEGF after PDT using chloro-aluminum phthalocyanine (AlClPc) entrapped in a lipid nanoemulsion in a split-mouth clinical trial. Material and methods Eight healthy volunteers with clinical indication for extraction were included in the study. Seven days before the extraction 40 ul of nanoemulsion AlClPc 5 μM was injected into gingival tissue followed by irradiation with diode laser, the contralateral side was used as control. Tissue specimens were removed seven days after the PDT and divided into two groups (test and control groups) for histological and immunohistochemical analysis. Patients were monitored at days, 0, 7, 14 and 30 to assess adverse effects of the therapy. Results The therapy was well tolerated by all patients. Adverse effects were short-time and completely reversible. Areas of edema, vascular congestion, and intense vascularization were viewed in gingival samples that received PDT. Additionally, dystrophic calcification was observed in subepithelial region. VEGF showed moderate to strong immunostaining in specimens subjected to PDT. Conclusions Taken together, the results showed that the protocol used in this study mediated by nanoemulsion containing AlClPc is safe for clinical application in gingival tissue and suggests that VEGF is increased after PDT. |
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Moraes, Maiara deVasconcelos, Roseane Carvalho deLongo, João Paulo FigueiróMuehlmann, Luis AlexandreAzevedo, Ricardo Bentes deLemos, Telma Maria Araújo MouraCosta, Antonio de Lisboa Lopes2017-05-16T13:30:42Z2017-05-16T13:30:42Z2015MORAES, M. et al. Effects of photodynamic therapy mediated by nanoemulsion containing chloro-aluminum phthalocyanine: a histologic and immunohistochemical study in human gingiva. Photodiagnosis and Photodynamic Therapy, v. 12, n. 4, p. 592-597, 2015.https://repositorio.ufrn.br/jspui/handle/123456789/22995http://doi.org/10.1016/j.pdpdt.2015.10.009engELSEVIERPhotodynamic therapyPhotochemotherapyPhotosensitizing agentsVascular endothelial growth Factor AEffects of photodynamic therapy mediated by nanoemulsion containing chloro-aluminum phthalocyanine: a histologic and immunohistochemical study in human gingivainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBackground Photodynamic therapy (PDT) uses photosensitizing agents, which are delivered in target cells, followed by local application of visible light in specific wavelengths. This reaction produce reactive oxygen species able to induce cell death by apoptosis or necrosis, injured to the local vasculature, and exert important effects on the immune system. Objective The present work evaluated the clinical findings, histomorphological alterations and immunodetection of VEGF after PDT using chloro-aluminum phthalocyanine (AlClPc) entrapped in a lipid nanoemulsion in a split-mouth clinical trial. Material and methods Eight healthy volunteers with clinical indication for extraction were included in the study. Seven days before the extraction 40 ul of nanoemulsion AlClPc 5 μM was injected into gingival tissue followed by irradiation with diode laser, the contralateral side was used as control. Tissue specimens were removed seven days after the PDT and divided into two groups (test and control groups) for histological and immunohistochemical analysis. Patients were monitored at days, 0, 7, 14 and 30 to assess adverse effects of the therapy. Results The therapy was well tolerated by all patients. Adverse effects were short-time and completely reversible. Areas of edema, vascular congestion, and intense vascularization were viewed in gingival samples that received PDT. Additionally, dystrophic calcification was observed in subepithelial region. VEGF showed moderate to strong immunostaining in specimens subjected to PDT. Conclusions Taken together, the results showed that the protocol used in this study mediated by nanoemulsion containing AlClPc is safe for clinical application in gingival tissue and suggests that VEGF is increased after PDT.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALEffectsPhotodynamicTherapy_Moraes_2015.pdfEffectsPhotodynamicTherapy_Moraes_2015.pdfhttp://www.sciencedirect.com/science/article/pii/S1572100015300417application/pdf2475388https://repositorio.ufrn.br/bitstream/123456789/22995/1/EffectsPhotodynamicTherapy_Moraes_2015.pdf4056aed877d02e0ceae2a5b31d7fa5b7MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81569https://repositorio.ufrn.br/bitstream/123456789/22995/2/license.txt6e6f57145bc87daf99079f06b081ff9fMD52TEXTEffects of photodynamic therapy mediated by nanoemulsion containing chloro-aluminum phthalocyanine_2015.pdf.txtEffects of photodynamic therapy mediated by nanoemulsion containing chloro-aluminum phthalocyanine_2015.pdf.txtExtracted texttext/plain36108https://repositorio.ufrn.br/bitstream/123456789/22995/5/Effects%20of%20photodynamic%20therapy%20mediated%20by%20nanoemulsion%20containing%20chloro-aluminum%20phthalocyanine_2015.pdf.txt162925f1d956d4ea22a9989eef93e870MD55THUMBNAILEffects of photodynamic therapy mediated by nanoemulsion containing chloro-aluminum phthalocyanine_2015.pdf.jpgEffects of photodynamic therapy mediated by nanoemulsion containing chloro-aluminum phthalocyanine_2015.pdf.jpgIM Thumbnailimage/jpeg7878https://repositorio.ufrn.br/bitstream/123456789/22995/6/Effects%20of%20photodynamic%20therapy%20mediated%20by%20nanoemulsion%20containing%20chloro-aluminum%20phthalocyanine_2015.pdf.jpg570e9e3e8120e9454a4cb3eeb8a93b4bMD56123456789/229952021-12-09 14:32:21.811oai:https://repositorio.ufrn.br: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ório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-12-09T17:32:21Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Effects of photodynamic therapy mediated by nanoemulsion containing chloro-aluminum phthalocyanine: a histologic and immunohistochemical study in human gingiva |
title |
Effects of photodynamic therapy mediated by nanoemulsion containing chloro-aluminum phthalocyanine: a histologic and immunohistochemical study in human gingiva |
spellingShingle |
Effects of photodynamic therapy mediated by nanoemulsion containing chloro-aluminum phthalocyanine: a histologic and immunohistochemical study in human gingiva Moraes, Maiara de Photodynamic therapy Photochemotherapy Photosensitizing agents Vascular endothelial growth Factor A |
title_short |
Effects of photodynamic therapy mediated by nanoemulsion containing chloro-aluminum phthalocyanine: a histologic and immunohistochemical study in human gingiva |
title_full |
Effects of photodynamic therapy mediated by nanoemulsion containing chloro-aluminum phthalocyanine: a histologic and immunohistochemical study in human gingiva |
title_fullStr |
Effects of photodynamic therapy mediated by nanoemulsion containing chloro-aluminum phthalocyanine: a histologic and immunohistochemical study in human gingiva |
title_full_unstemmed |
Effects of photodynamic therapy mediated by nanoemulsion containing chloro-aluminum phthalocyanine: a histologic and immunohistochemical study in human gingiva |
title_sort |
Effects of photodynamic therapy mediated by nanoemulsion containing chloro-aluminum phthalocyanine: a histologic and immunohistochemical study in human gingiva |
author |
Moraes, Maiara de |
author_facet |
Moraes, Maiara de Vasconcelos, Roseane Carvalho de Longo, João Paulo Figueiró Muehlmann, Luis Alexandre Azevedo, Ricardo Bentes de Lemos, Telma Maria Araújo Moura Costa, Antonio de Lisboa Lopes |
author_role |
author |
author2 |
Vasconcelos, Roseane Carvalho de Longo, João Paulo Figueiró Muehlmann, Luis Alexandre Azevedo, Ricardo Bentes de Lemos, Telma Maria Araújo Moura Costa, Antonio de Lisboa Lopes |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Moraes, Maiara de Vasconcelos, Roseane Carvalho de Longo, João Paulo Figueiró Muehlmann, Luis Alexandre Azevedo, Ricardo Bentes de Lemos, Telma Maria Araújo Moura Costa, Antonio de Lisboa Lopes |
dc.subject.por.fl_str_mv |
Photodynamic therapy Photochemotherapy Photosensitizing agents Vascular endothelial growth Factor A |
topic |
Photodynamic therapy Photochemotherapy Photosensitizing agents Vascular endothelial growth Factor A |
description |
Background Photodynamic therapy (PDT) uses photosensitizing agents, which are delivered in target cells, followed by local application of visible light in specific wavelengths. This reaction produce reactive oxygen species able to induce cell death by apoptosis or necrosis, injured to the local vasculature, and exert important effects on the immune system. Objective The present work evaluated the clinical findings, histomorphological alterations and immunodetection of VEGF after PDT using chloro-aluminum phthalocyanine (AlClPc) entrapped in a lipid nanoemulsion in a split-mouth clinical trial. Material and methods Eight healthy volunteers with clinical indication for extraction were included in the study. Seven days before the extraction 40 ul of nanoemulsion AlClPc 5 μM was injected into gingival tissue followed by irradiation with diode laser, the contralateral side was used as control. Tissue specimens were removed seven days after the PDT and divided into two groups (test and control groups) for histological and immunohistochemical analysis. Patients were monitored at days, 0, 7, 14 and 30 to assess adverse effects of the therapy. Results The therapy was well tolerated by all patients. Adverse effects were short-time and completely reversible. Areas of edema, vascular congestion, and intense vascularization were viewed in gingival samples that received PDT. Additionally, dystrophic calcification was observed in subepithelial region. VEGF showed moderate to strong immunostaining in specimens subjected to PDT. Conclusions Taken together, the results showed that the protocol used in this study mediated by nanoemulsion containing AlClPc is safe for clinical application in gingival tissue and suggests that VEGF is increased after PDT. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015 |
dc.date.accessioned.fl_str_mv |
2017-05-16T13:30:42Z |
dc.date.available.fl_str_mv |
2017-05-16T13:30:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MORAES, M. et al. Effects of photodynamic therapy mediated by nanoemulsion containing chloro-aluminum phthalocyanine: a histologic and immunohistochemical study in human gingiva. Photodiagnosis and Photodynamic Therapy, v. 12, n. 4, p. 592-597, 2015. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/jspui/handle/123456789/22995 |
dc.identifier.doi.none.fl_str_mv |
http://doi.org/10.1016/j.pdpdt.2015.10.009 |
identifier_str_mv |
MORAES, M. et al. Effects of photodynamic therapy mediated by nanoemulsion containing chloro-aluminum phthalocyanine: a histologic and immunohistochemical study in human gingiva. Photodiagnosis and Photodynamic Therapy, v. 12, n. 4, p. 592-597, 2015. |
url |
https://repositorio.ufrn.br/jspui/handle/123456789/22995 http://doi.org/10.1016/j.pdpdt.2015.10.009 |
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eng |
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