Cryopreservation in liquid nitrogen of Brazilian orchid seeds Miltonia flavescens Lindl.
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Agro@mbiente on-line |
Texto Completo: | https://revista.ufrr.br/agroambiente/article/view/6388 |
Resumo: | Miltonia flavescens is a species vulnerable to extinction, which justifies research on preservation methods. Cryopreservation in liquid nitrogen (LN) consists of maintaining biological material at a low-temperature (-196 °C). Thus, the aim of the experiment is to evaluate the influence of different cryoprotective solutions on cryopreservation in LN of the Brazilian orchid Miltonia flavescens seeds. The experiment was conducted in a completely randomized design (CRD), with eight treatments and six replications. The treatments were composed as follows: control; immersion in LN, no cryoprotectant adding; and immersion in LN, with the addition of cryoprotectants: sucrose 0,4 mol L-1; glycerol 2 mol L-1; protection by vitrification in solution (PVS)1; PVS2; PVS2 + 1% phloroglucinol, and PVS3. Except for the control treatment, which was kept in a freezer (10±2 °C), the others remained frozen for 15 days. After this period, the viability of the seeds was evaluated. These seeds were sown and, 30 days after germination, then the frequency of protocorm formation was verified. Before the cryopreservation, the seeds showed 75% viability and 9.5% water content. After cryopreservation, the seeds varied between 67 to 75% viability. However, treatment with glycerol 2 mol L-1 exhibited lower performance than the others (58%). The control treatment showed a higher percentage of protocorm formation (71%) followed by treatments PVS1 (63%), PVS2 (64%), and PVS2PHLO (66%). For the purpose of preserving Miltonia flavescens seeds in liquid nitrogen for a prolonged period, the treatments PVS1, PVS2, and PVS2PHLO proved to be viable and promising alternatives. |
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Cryopreservation in liquid nitrogen of Brazilian orchid seeds Miltonia flavescens Lindl.Seed storage. Conservation. Orchidaceae.Miltonia flavescens is a species vulnerable to extinction, which justifies research on preservation methods. Cryopreservation in liquid nitrogen (LN) consists of maintaining biological material at a low-temperature (-196 °C). Thus, the aim of the experiment is to evaluate the influence of different cryoprotective solutions on cryopreservation in LN of the Brazilian orchid Miltonia flavescens seeds. The experiment was conducted in a completely randomized design (CRD), with eight treatments and six replications. The treatments were composed as follows: control; immersion in LN, no cryoprotectant adding; and immersion in LN, with the addition of cryoprotectants: sucrose 0,4 mol L-1; glycerol 2 mol L-1; protection by vitrification in solution (PVS)1; PVS2; PVS2 + 1% phloroglucinol, and PVS3. Except for the control treatment, which was kept in a freezer (10±2 °C), the others remained frozen for 15 days. After this period, the viability of the seeds was evaluated. These seeds were sown and, 30 days after germination, then the frequency of protocorm formation was verified. Before the cryopreservation, the seeds showed 75% viability and 9.5% water content. After cryopreservation, the seeds varied between 67 to 75% viability. However, treatment with glycerol 2 mol L-1 exhibited lower performance than the others (58%). The control treatment showed a higher percentage of protocorm formation (71%) followed by treatments PVS1 (63%), PVS2 (64%), and PVS2PHLO (66%). For the purpose of preserving Miltonia flavescens seeds in liquid nitrogen for a prolonged period, the treatments PVS1, PVS2, and PVS2PHLO proved to be viable and promising alternatives.UFRR2020-11-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revista.ufrr.br/agroambiente/article/view/638810.18227/1982-8470ragro.v14i0.6388AGRO@MBIENTE ON-LINE JOURNALRAGR; Vol. 14 (2020)REVISTA AGRO@MBIENTE ON-LINE; Vol. 14 (2020)REVISTA AGRO@MBIENTE ON-LINE; v. 14 (2020)1982-8470reponame:Agro@mbiente on-lineinstname:Universidade Federal de Roraima (UFRR)instacron:UFRRporhttps://revista.ufrr.br/agroambiente/article/view/6388/3191Copyright (c) 2020 REVISTA AGRO@MBIENTE ON-LINEinfo:eu-repo/semantics/openAccessBaudraz de Paula, Jean CarloRibeiro Júnior, Walter AparecidoShimizu, Gabriel DaniloMen, Gabriel Barracade Faria, Ricardo Tadeu2020-12-16T13:47:07Zoai:oai.revista.ufrr.br:article/6388Revistahttps://revista.ufrr.br/index.php/agroambientePUBhttps://revista.ufrr.br/index.php/agroambiente/oai||scpuchoa@dsi.ufrr.br|| arcanjoalves@oi.com.br1982-84701982-8470opendoar:2020-12-16T13:47:07Agro@mbiente on-line - Universidade Federal de Roraima (UFRR)false |
dc.title.none.fl_str_mv |
Cryopreservation in liquid nitrogen of Brazilian orchid seeds Miltonia flavescens Lindl. |
title |
Cryopreservation in liquid nitrogen of Brazilian orchid seeds Miltonia flavescens Lindl. |
spellingShingle |
Cryopreservation in liquid nitrogen of Brazilian orchid seeds Miltonia flavescens Lindl. Baudraz de Paula, Jean Carlo Seed storage. Conservation. Orchidaceae. |
title_short |
Cryopreservation in liquid nitrogen of Brazilian orchid seeds Miltonia flavescens Lindl. |
title_full |
Cryopreservation in liquid nitrogen of Brazilian orchid seeds Miltonia flavescens Lindl. |
title_fullStr |
Cryopreservation in liquid nitrogen of Brazilian orchid seeds Miltonia flavescens Lindl. |
title_full_unstemmed |
Cryopreservation in liquid nitrogen of Brazilian orchid seeds Miltonia flavescens Lindl. |
title_sort |
Cryopreservation in liquid nitrogen of Brazilian orchid seeds Miltonia flavescens Lindl. |
author |
Baudraz de Paula, Jean Carlo |
author_facet |
Baudraz de Paula, Jean Carlo Ribeiro Júnior, Walter Aparecido Shimizu, Gabriel Danilo Men, Gabriel Barraca de Faria, Ricardo Tadeu |
author_role |
author |
author2 |
Ribeiro Júnior, Walter Aparecido Shimizu, Gabriel Danilo Men, Gabriel Barraca de Faria, Ricardo Tadeu |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Baudraz de Paula, Jean Carlo Ribeiro Júnior, Walter Aparecido Shimizu, Gabriel Danilo Men, Gabriel Barraca de Faria, Ricardo Tadeu |
dc.subject.por.fl_str_mv |
Seed storage. Conservation. Orchidaceae. |
topic |
Seed storage. Conservation. Orchidaceae. |
description |
Miltonia flavescens is a species vulnerable to extinction, which justifies research on preservation methods. Cryopreservation in liquid nitrogen (LN) consists of maintaining biological material at a low-temperature (-196 °C). Thus, the aim of the experiment is to evaluate the influence of different cryoprotective solutions on cryopreservation in LN of the Brazilian orchid Miltonia flavescens seeds. The experiment was conducted in a completely randomized design (CRD), with eight treatments and six replications. The treatments were composed as follows: control; immersion in LN, no cryoprotectant adding; and immersion in LN, with the addition of cryoprotectants: sucrose 0,4 mol L-1; glycerol 2 mol L-1; protection by vitrification in solution (PVS)1; PVS2; PVS2 + 1% phloroglucinol, and PVS3. Except for the control treatment, which was kept in a freezer (10±2 °C), the others remained frozen for 15 days. After this period, the viability of the seeds was evaluated. These seeds were sown and, 30 days after germination, then the frequency of protocorm formation was verified. Before the cryopreservation, the seeds showed 75% viability and 9.5% water content. After cryopreservation, the seeds varied between 67 to 75% viability. However, treatment with glycerol 2 mol L-1 exhibited lower performance than the others (58%). The control treatment showed a higher percentage of protocorm formation (71%) followed by treatments PVS1 (63%), PVS2 (64%), and PVS2PHLO (66%). For the purpose of preserving Miltonia flavescens seeds in liquid nitrogen for a prolonged period, the treatments PVS1, PVS2, and PVS2PHLO proved to be viable and promising alternatives. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11-27 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revista.ufrr.br/agroambiente/article/view/6388 10.18227/1982-8470ragro.v14i0.6388 |
url |
https://revista.ufrr.br/agroambiente/article/view/6388 |
identifier_str_mv |
10.18227/1982-8470ragro.v14i0.6388 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revista.ufrr.br/agroambiente/article/view/6388/3191 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 REVISTA AGRO@MBIENTE ON-LINE info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 REVISTA AGRO@MBIENTE ON-LINE |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UFRR |
publisher.none.fl_str_mv |
UFRR |
dc.source.none.fl_str_mv |
AGRO@MBIENTE ON-LINE JOURNALRAGR; Vol. 14 (2020) REVISTA AGRO@MBIENTE ON-LINE; Vol. 14 (2020) REVISTA AGRO@MBIENTE ON-LINE; v. 14 (2020) 1982-8470 reponame:Agro@mbiente on-line instname:Universidade Federal de Roraima (UFRR) instacron:UFRR |
instname_str |
Universidade Federal de Roraima (UFRR) |
instacron_str |
UFRR |
institution |
UFRR |
reponame_str |
Agro@mbiente on-line |
collection |
Agro@mbiente on-line |
repository.name.fl_str_mv |
Agro@mbiente on-line - Universidade Federal de Roraima (UFRR) |
repository.mail.fl_str_mv |
||scpuchoa@dsi.ufrr.br|| arcanjoalves@oi.com.br |
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1799770041695076352 |