An?lise da influ?ncia de plantas transg?nicas em Urbanus acawoios (Williams, 1926) (Lepidoptera: Hesperiidae) e em Galleria mellonella (Linnaeus, 1758) (Lepidoptera: Pyralidae)

• Autor(a) principal: Trevisan, Henrique
• Data de Publicação: 2010
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
• Texto Completo: https://tede.ufrrj.br/jspui/handle/tede/487
• topic: transg?nicos; insetos n?o alvo; herbicida; Bacillus thuringiensis; transgenic; non-target insect; herbicide; Recursos Florestais e Engenharia Florestal

This study was conducted in two stages, with different experiments and culminating in two chapters. The first focuses on the evaluation of the influence of the complex technology used in transgenic soybean in the biological aspects of Urbanus acawoios (Williams, 1926) (Lepidoptera: Hesperiidae). Four plots of soybeans were grown, three of genetically modified soybean cultivar P98R62 (soybean GMRR) and with a conventional soybean cultivar BRS 232 (Soybean conv.). Only two plots with transgenic herbicide were applied at a concentration of 1200 g ai ha-1, the Roundup Ready ? and Roundup ? WG, both applied twice, 25 and 45 days after emergence. 125 eggs coming from a butterfly rearing in the open air, and from the same female, were used to assembly the five treatments each with 25 larvae, which are made of larvae fed on leaves of Soybean conv., Soy GMRR without herbicide application, soy GMRR treated with glyphosate in Roundup Ready ?, GMRR treated with glyphosate in Roundup ? WG and leaves of C. fairchildiana. The mortality rate of larvae was higher in treatments containing soy GMRR, with or without herbicide, when compared with the mortality of larvae fed with C. fairchildiana and soybean conv. The pupae originated from larvae fed on soy conv. and C. fairchildiana were shown 100% viable, as coming from the GMRR treated with Roundup Ready ?, Roundup ? WG and without herbicide, were shown up 84.6%, 87.5% and 88.2% viable, respectively. The second chapter deals with an assessment of the influence of pollen from transgenic corn resistant to insects on Galleria mellonella (Linnaeus, 1758) (Lepidoptera: Pyralidae). This is a lepidopteran pyralids associated with beehives; their larvae feed on old wax, pollen, exuviae, etc. There are conflicting opinions about its safety to bees, beekeeping and therefore, to consider a pest of apiaries, others to classify as an important organism in the recycling of organic matter inside the hive. This last view may be considered beneficial to bees. Within this context, the adoption of transgenic crops resistant to insects by expressing the Bt protein from Bacillus thuringiensis, may cause disease of the larvae of G. mellonella. Bees to collect pollen and store in the hives can expose this lepidopteran to intoxication by Bt proteins present in pollen. This study aims to evaluate the influence of Bt corn pollen on G. mellonella and then trace a discussion about the possibility of the condition field of Lepidoptera. For this a bioassay was performed, where it was offered to the larvae pollen Bt, conventional and commercial. The duration of the larval stage of G. mellonella was affected by the supply of pollen, and the corn by conventional or transgenic, provided the biggest records. Mortality was higher in individuals fed with transgenic pollen and bee wax, but did not differ from other foods, when pollen from Bt was offered honeycomb of Apis mellifera. The width and length of larvae originated from larvae fed with bee wax added to conventional or transgenic pollen were lower than other treatments. The corn pollen, conventional or GM alone is a less suitable food for the development of G. mellonella, in relation to a diet consisting of pollen from various plants.