Diagn?stico da Anemia Infecciosa Eq?ina: an?lise comparativa de sistemas comerciais de diagn?stico por imunodifus?o
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRRJ |
Texto Completo: | https://tede.ufrrj.br/jspui/handle/tede/866 |
Resumo: | Brazil has currently the second bigger flock of equines of the world and the brazilian equines breeding plays an important role in the development of the sector of agribusiness. Among the infectious disease that affect the national equines breeding, the Equine infectious anemia (EIAV) has been shown of difficult control. The Equine infectious anemia is caused by a retrovirus, it has a worldwide distribution, and it is recognized as the most important disease of equines. The most common clinical signals in the acute phase, are anemia followed of jaundice in the mucosae, ventral oedema, mioglobinury, caquexy and, mainly, intermittent fever. Once it has no treatment, Ministry of Agriculture, Cattle and Supplying determines, through legal mechanisms, a politics of obligatory examination in credentialed laboratories, for the transport and commercialization of equines in the country. The examination is based on the test of Coggins, an immunodiffusion in agar gel (IDGA) of the serum of the animal tested against antigen of the EIAV. As the infection is lifetime, positive animals are euthanasiated by Veterinarians of the Ministry of Agriculture. Considering that precision of the results is critical, once that false positives animals could uselessly be sacrificed, and false negatives will be preserved as infection source, diverse problems may occur, appeared the interest of a systematic analysis of the reproducibility of kits and on possible sources of error. For this purpose, positive and negative sera, referred by the repetition of the tests for Coggins and ELISA, had been evaluated by IDGA comparatively with three kits of different manufacturers. In the experiments of reproducibility, it observed a great distinction in the quality of the precipitation line promoted for the positive sera in the tests. This phenomenon may be justified by the use of the conditions techniques legal, determined for would carry of the Ministry of Agriculture that is distinct of the recommendation of the analyzed manufacturers of two of kits. Exactly thus, the evaluation of the reproducibility of the results of kits with the referred sera showed high correlation. |
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Mazur, Carlos610.764.147-53http://lattes.cnpq.br/7059968849728033Danelli, Maria das Gracas Miranda758.643.637-15http://lattes.cnpq.br/7414712085904286580.365.987-87http://lattes.cnpq.br/6798409174173105Silva, Antonia Regina Sessa da2016-04-28T20:17:27Z2010-10-272007-03-30SILVA, Antonia Regina Sessa da. Diagn?stico da Anemia Infecciosa Eq?ina: an?lise comparativa de sistemas comerciais de diagn?stico por imunodifus?o. 2007. 66 f. Disserta??o (Mestrado em Microbiologia Veterin?ria) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2007.https://tede.ufrrj.br/jspui/handle/tede/866Brazil has currently the second bigger flock of equines of the world and the brazilian equines breeding plays an important role in the development of the sector of agribusiness. Among the infectious disease that affect the national equines breeding, the Equine infectious anemia (EIAV) has been shown of difficult control. The Equine infectious anemia is caused by a retrovirus, it has a worldwide distribution, and it is recognized as the most important disease of equines. The most common clinical signals in the acute phase, are anemia followed of jaundice in the mucosae, ventral oedema, mioglobinury, caquexy and, mainly, intermittent fever. Once it has no treatment, Ministry of Agriculture, Cattle and Supplying determines, through legal mechanisms, a politics of obligatory examination in credentialed laboratories, for the transport and commercialization of equines in the country. The examination is based on the test of Coggins, an immunodiffusion in agar gel (IDGA) of the serum of the animal tested against antigen of the EIAV. As the infection is lifetime, positive animals are euthanasiated by Veterinarians of the Ministry of Agriculture. Considering that precision of the results is critical, once that false positives animals could uselessly be sacrificed, and false negatives will be preserved as infection source, diverse problems may occur, appeared the interest of a systematic analysis of the reproducibility of kits and on possible sources of error. For this purpose, positive and negative sera, referred by the repetition of the tests for Coggins and ELISA, had been evaluated by IDGA comparatively with three kits of different manufacturers. In the experiments of reproducibility, it observed a great distinction in the quality of the precipitation line promoted for the positive sera in the tests. This phenomenon may be justified by the use of the conditions techniques legal, determined for would carry of the Ministry of Agriculture that is distinct of the recommendation of the analyzed manufacturers of two of kits. Exactly thus, the evaluation of the reproducibility of the results of kits with the referred sera showed high correlation.O Brasil possui atualmente o segundo maior rebanho de eq??deos do mundo e a Eq?ideocultura Brasileira desempenha um importante papel no desenvolvimento do setor de agroneg?cios. Entre as doen?as infecciosas que afetam a eq?inocultura nacional, a Anemia Infecciosa dos Eq?inos (AIE) tem se mostrado de dif?cil controle. A AIE ? causada por um retrov?rus, apresenta uma distribui??o mundial, e ? reconhecida como a mais importante doen?a dos eq?inos. Entre os sinais cl?nicos mais comuns, na fase aguda da doen?a, encontram-se a anemia seguida de icter?cia nas mucosas, edema ventral, mioglobin?ria, caquexia e, principalmente, febre intermitente. Como n?o h? tratamento, Minist?rio da Agricultura, Pecu?ria e Abastecimento (MAPA) determina, atrav?s de mecanismo legais, uma pol?tica de exame obrigat?rio em laborat?rios credenciados, para o transporte e comercializa??o de eq??deos no Pa?s. O exame ? baseado na prova de Coggins, uma imunodifus?o em ?gar gel do soro do animal testado contra ant?geno do v?rus da AIE. Como a infec??o ? vital?cia, animais soropositivos s?o eutanasiados por M?dicos Veterin?rios do MAPA. Considerando que a precis?o dos resultados ? cr?tica, posto que animais falso positivos podem ser sacrificados inutilmente, e falso negativos preservados como fonte de infec??o, surgiu o interesse de um an?lise sistem?tica da reprodutibilidade dos kits e poss?veis fontes de erro no diagn?stico. Para esta finalidade, soros positivos e negativos, referenciados pela repeti??o dos testes de Coggins e ELISA, foram avaliados pela prova de IDGA, comparativamente com tr?s kits de diferentes fabricantes. Nos experimentos de reprodutibilidade, observou-se uma grande distin??o na qualidade da linha de precipita??o promovida pelos soros positivos nos testes nos diferentes kits. Este fen?meno pode ser justificado pela utiliza??o das condi??es t?cnicas legais, determinadas por portaria do MAPA, que s?o distintas das recomenda??es dos fabricantes de dois dos kits analisados. Mesmo assim, a avalia??o da reprodutibilidade dos resultados dos kits com os soros referenciados mostrou elevada correla??o.Made available in DSpace on 2016-04-28T20:17:27Z (GMT). No. of bitstreams: 1 2007 - Antonia Regina Sessa da Silva.pdf: 384094 bytes, checksum: 91d2147f8402249dead112e338e89b66 (MD5) Previous issue date: 2007-03-30application/pdfhttps://tede.ufrrj.br/retrieve/60240/2007%20-%20Antonia%20Regina%20Sessa%20da%20Silva.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Microbiologia Veterin?riaUFRRJBrasilInstituto de Veterin?riaanemia infecciosa eq?inaeq?inoseq??deoslentivirusretrovirusdiagn?stico e IDGAequine infectious anemiaequineslentivirusretrovirusMedicina Veterin?riaDiagn?stico da Anemia Infecciosa Eq?ina: an?lise comparativa de sistemas comerciais de diagn?stico por imunodifus?oinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRRJinstname:Universidade Federal Rural do Rio de Janeiro (UFRRJ)instacron:UFRRJTHUMBNAIL2007 - Antonia Regina Sessa da Silva.pdf.jpg2007 - Antonia Regina Sessa da Silva.pdf.jpgimage/jpeg2085http://localhost:8080/tede/bitstream/tede/866/3/2007+-+Antonia+Regina+Sessa+da+Silva.pdf.jpg586f6fe88c5b8364be61f7cd09e3e4ecMD53TEXT2007 - Antonia Regina Sessa da Silva.pdf.txt2007 - Antonia Regina Sessa da Silva.pdf.txttext/plain109065http://localhost:8080/tede/bitstream/tede/866/2/2007+-+Antonia+Regina+Sessa+da+Silva.pdf.txtbdec3c7737007f6c383b3fde34c17894MD52ORIGINAL2007 - Antonia Regina Sessa da Silva.pdf2007 - Antonia Regina Sessa da Silva.pdfapplication/pdf384808http://localhost:8080/tede/bitstream/tede/866/1/2007+-+Antonia+Regina+Sessa+da+Silva.pdfe99a43d7fb0628cdf25b75dddc0731b5MD51tede/8662022-06-13 11:38:21.4oai:localhost:tede/866Biblioteca Digital de Teses e Dissertaçõeshttps://tede.ufrrj.br/PUBhttps://tede.ufrrj.br/oai/requestbibliot@ufrrj.br||bibliot@ufrrj.bropendoar:2022-06-13T14:38:21Biblioteca Digital de Teses e Dissertações da UFRRJ - Universidade Federal Rural do Rio de Janeiro (UFRRJ)false |
dc.title.por.fl_str_mv |
Diagn?stico da Anemia Infecciosa Eq?ina: an?lise comparativa de sistemas comerciais de diagn?stico por imunodifus?o |
title |
Diagn?stico da Anemia Infecciosa Eq?ina: an?lise comparativa de sistemas comerciais de diagn?stico por imunodifus?o |
spellingShingle |
Diagn?stico da Anemia Infecciosa Eq?ina: an?lise comparativa de sistemas comerciais de diagn?stico por imunodifus?o Silva, Antonia Regina Sessa da anemia infecciosa eq?ina eq?inos eq??deos lentivirus retrovirus diagn?stico e IDGA equine infectious anemia equines lentivirus retrovirus Medicina Veterin?ria |
title_short |
Diagn?stico da Anemia Infecciosa Eq?ina: an?lise comparativa de sistemas comerciais de diagn?stico por imunodifus?o |
title_full |
Diagn?stico da Anemia Infecciosa Eq?ina: an?lise comparativa de sistemas comerciais de diagn?stico por imunodifus?o |
title_fullStr |
Diagn?stico da Anemia Infecciosa Eq?ina: an?lise comparativa de sistemas comerciais de diagn?stico por imunodifus?o |
title_full_unstemmed |
Diagn?stico da Anemia Infecciosa Eq?ina: an?lise comparativa de sistemas comerciais de diagn?stico por imunodifus?o |
title_sort |
Diagn?stico da Anemia Infecciosa Eq?ina: an?lise comparativa de sistemas comerciais de diagn?stico por imunodifus?o |
author |
Silva, Antonia Regina Sessa da |
author_facet |
Silva, Antonia Regina Sessa da |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Mazur, Carlos |
dc.contributor.advisor1ID.fl_str_mv |
610.764.147-53 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/7059968849728033 |
dc.contributor.advisor-co1.fl_str_mv |
Danelli, Maria das Gracas Miranda |
dc.contributor.advisor-co1ID.fl_str_mv |
758.643.637-15 |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/7414712085904286 |
dc.contributor.authorID.fl_str_mv |
580.365.987-87 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/6798409174173105 |
dc.contributor.author.fl_str_mv |
Silva, Antonia Regina Sessa da |
contributor_str_mv |
Mazur, Carlos Danelli, Maria das Gracas Miranda |
dc.subject.por.fl_str_mv |
anemia infecciosa eq?ina eq?inos eq??deos lentivirus retrovirus diagn?stico e IDGA |
topic |
anemia infecciosa eq?ina eq?inos eq??deos lentivirus retrovirus diagn?stico e IDGA equine infectious anemia equines lentivirus retrovirus Medicina Veterin?ria |
dc.subject.eng.fl_str_mv |
equine infectious anemia equines lentivirus retrovirus |
dc.subject.cnpq.fl_str_mv |
Medicina Veterin?ria |
description |
Brazil has currently the second bigger flock of equines of the world and the brazilian equines breeding plays an important role in the development of the sector of agribusiness. Among the infectious disease that affect the national equines breeding, the Equine infectious anemia (EIAV) has been shown of difficult control. The Equine infectious anemia is caused by a retrovirus, it has a worldwide distribution, and it is recognized as the most important disease of equines. The most common clinical signals in the acute phase, are anemia followed of jaundice in the mucosae, ventral oedema, mioglobinury, caquexy and, mainly, intermittent fever. Once it has no treatment, Ministry of Agriculture, Cattle and Supplying determines, through legal mechanisms, a politics of obligatory examination in credentialed laboratories, for the transport and commercialization of equines in the country. The examination is based on the test of Coggins, an immunodiffusion in agar gel (IDGA) of the serum of the animal tested against antigen of the EIAV. As the infection is lifetime, positive animals are euthanasiated by Veterinarians of the Ministry of Agriculture. Considering that precision of the results is critical, once that false positives animals could uselessly be sacrificed, and false negatives will be preserved as infection source, diverse problems may occur, appeared the interest of a systematic analysis of the reproducibility of kits and on possible sources of error. For this purpose, positive and negative sera, referred by the repetition of the tests for Coggins and ELISA, had been evaluated by IDGA comparatively with three kits of different manufacturers. In the experiments of reproducibility, it observed a great distinction in the quality of the precipitation line promoted for the positive sera in the tests. This phenomenon may be justified by the use of the conditions techniques legal, determined for would carry of the Ministry of Agriculture that is distinct of the recommendation of the analyzed manufacturers of two of kits. Exactly thus, the evaluation of the reproducibility of the results of kits with the referred sera showed high correlation. |
publishDate |
2007 |
dc.date.issued.fl_str_mv |
2007-03-30 |
dc.date.available.fl_str_mv |
2010-10-27 |
dc.date.accessioned.fl_str_mv |
2016-04-28T20:17:27Z |
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info:eu-repo/semantics/publishedVersion |
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dc.identifier.citation.fl_str_mv |
SILVA, Antonia Regina Sessa da. Diagn?stico da Anemia Infecciosa Eq?ina: an?lise comparativa de sistemas comerciais de diagn?stico por imunodifus?o. 2007. 66 f. Disserta??o (Mestrado em Microbiologia Veterin?ria) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2007. |
dc.identifier.uri.fl_str_mv |
https://tede.ufrrj.br/jspui/handle/tede/866 |
identifier_str_mv |
SILVA, Antonia Regina Sessa da. Diagn?stico da Anemia Infecciosa Eq?ina: an?lise comparativa de sistemas comerciais de diagn?stico por imunodifus?o. 2007. 66 f. Disserta??o (Mestrado em Microbiologia Veterin?ria) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2007. |
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UFRRJ |
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Brasil |
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Universidade Federal Rural do Rio de Janeiro |
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