Isolamento e caracteriza??o molecular de duas novas cepas de Anaplasma marginale e estabelecimento de linhagens de c?lulas embrion?rias do carrapato Rhipicephalus (Boophilus) microplus

Detalhes bibliográficos
Autor(a) principal: Ba?ta, Bruna de Azevedo
Data de Publicação: 2015
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://tede.ufrrj.br/jspui/handle/jspui/2753
Resumo: In the first chapter two new strains of Anaplasma marginale derived from the Serop?dica, RJ were isolated and characterized in tick cells line of Ixodes scapularis (IDE8), enabling new studies in the diagnosis with the use of local strains. In the second chapter the first lines of embryonic cells of Rhipicephalus (Boophilus) microplus from Brazilian strains were developed and characterized. The strains of A. marginale were from blood of two naturally infected calves belonging to the Universidade Federal Rural do Rio de Janeiro (UFRRJ), Serop?dica. After isolation, the strains were characterized morphologically by light microscopy and molecularly by nested PCR of msp1? gene. Two strains of A. marginale were isolated and named as AmRio1 and AmRio2. Small colonies were observed in cytospin smears stained with Giemsa?s 1:10, of each of the isolates, 60 days after culture initiation. Analysis of msp1? microsatellite in both strains resulted in a single genotype, genotype E. The amino acid sequence of one MSP1? tandem repeat from the strain AmRio1 resulted in a new sequence (named 162) with one amino acid change. The results of our phylogenetic analysis demonstrated that A. marginale strains from Brazil and Argentina formed two large clusters. In establishing of the strains of R. (B.) microplus, the ticks were from strain kept calves in Parasitic Diseases Laboratory, UFRRJ, Serop?dica. After 13 days of laying, the pool of eggs was sterilized, macerated, filtered and centrifuged at 200 xg for 10 minutes. The pellet was suspended in Leibotiz?s L15 medium and divided into 6 flasks of 25 cm2, of which 3 were contaminated, one was deposited in the cell bank of the Pirbright Institute and others two were characterized in the present study (BMEBR1 and BMEBR2 lines) through molecular analysis of the 16S rDNA gene (PCR), growth curve and epifluorescence and light microscopy. Sixty days in culture, the cells began to show confluent aspect, reaching the formation of the monolayer. There was a complete similarity (100%) of new lines BMEBR1 and BMEBR2 with R. (B.) microplus in Brazil (EU918178.1), Costa Rica (EU918179.1), Paraguay (EU918180.1), among others. Both strains showed lower growth than the R. (B.) microplus line from Costa Rica, BME/CTVM2. The BMEBR1 line has increased phagocytic ability that the BMEBR2 and BME/CTVM2 lines. In addition, these strains have acidification characteristics, lipid corpuscle production and oxidative stress different from each other. The new strains were frozen and defrosted successfully, and were stable with respect to morphology and growth rate. Thus, these lines can be used in future studies to propagation of new strains of A. marginale isolated.
id UFRRJ-1_62b0d8517189540af34a0e49bede1976
oai_identifier_str oai:localhost:jspui/2753
network_acronym_str UFRRJ-1
network_name_str Biblioteca Digital de Teses e Dissertações da UFRRJ
repository_id_str
spelling Fonseca, Adivaldo Henrique da475.018.557-49http://lattes.cnpq.br/4411441162862608Zweygarth, ErichPiranda, Eliane MattosDecker, Lygia MariaBaldani, Cristiane DivanSantos, Huarrisson Azevedo101.354.697-08http://lattes.cnpq.br/8016012971743463Ba?ta, Bruna de Azevedo2019-06-18T15:57:31Z2015-02-20Ba?ta, Bruna de Azevedo. Isolamento e caracteriza??o molecular de duas novas cepas de Anaplasma marginale e estabelecimento de linhagens de c?lulas embrion?rias do carrapato Rhipicephalus (Boophilus) microplus. 2015. [98 f.]. Tese( Programa de P?s-Gradua??o em Ci?ncias Veterin?rias) - Universidade Federal Rural do Rio de Janeiro, [Serop?dica - Rio de Janeiro] .https://tede.ufrrj.br/jspui/handle/jspui/2753In the first chapter two new strains of Anaplasma marginale derived from the Serop?dica, RJ were isolated and characterized in tick cells line of Ixodes scapularis (IDE8), enabling new studies in the diagnosis with the use of local strains. In the second chapter the first lines of embryonic cells of Rhipicephalus (Boophilus) microplus from Brazilian strains were developed and characterized. The strains of A. marginale were from blood of two naturally infected calves belonging to the Universidade Federal Rural do Rio de Janeiro (UFRRJ), Serop?dica. After isolation, the strains were characterized morphologically by light microscopy and molecularly by nested PCR of msp1? gene. Two strains of A. marginale were isolated and named as AmRio1 and AmRio2. Small colonies were observed in cytospin smears stained with Giemsa?s 1:10, of each of the isolates, 60 days after culture initiation. Analysis of msp1? microsatellite in both strains resulted in a single genotype, genotype E. The amino acid sequence of one MSP1? tandem repeat from the strain AmRio1 resulted in a new sequence (named 162) with one amino acid change. The results of our phylogenetic analysis demonstrated that A. marginale strains from Brazil and Argentina formed two large clusters. In establishing of the strains of R. (B.) microplus, the ticks were from strain kept calves in Parasitic Diseases Laboratory, UFRRJ, Serop?dica. After 13 days of laying, the pool of eggs was sterilized, macerated, filtered and centrifuged at 200 xg for 10 minutes. The pellet was suspended in Leibotiz?s L15 medium and divided into 6 flasks of 25 cm2, of which 3 were contaminated, one was deposited in the cell bank of the Pirbright Institute and others two were characterized in the present study (BMEBR1 and BMEBR2 lines) through molecular analysis of the 16S rDNA gene (PCR), growth curve and epifluorescence and light microscopy. Sixty days in culture, the cells began to show confluent aspect, reaching the formation of the monolayer. There was a complete similarity (100%) of new lines BMEBR1 and BMEBR2 with R. (B.) microplus in Brazil (EU918178.1), Costa Rica (EU918179.1), Paraguay (EU918180.1), among others. Both strains showed lower growth than the R. (B.) microplus line from Costa Rica, BME/CTVM2. The BMEBR1 line has increased phagocytic ability that the BMEBR2 and BME/CTVM2 lines. In addition, these strains have acidification characteristics, lipid corpuscle production and oxidative stress different from each other. The new strains were frozen and defrosted successfully, and were stable with respect to morphology and growth rate. Thus, these lines can be used in future studies to propagation of new strains of A. marginale isolated.No primeiro cap?tulo foram isoladas e caracterizadas duas novas cepas de Anaplasma marginale oriundas do munic?pio de Serop?dica, RJ, em linhagem de c?lulas de carrapato Ixodes scapularis (IDE8), possibilitando novos estudos no diagn?stico com a utiliza??o de cepas locais. No segundo cap?tulo foram desenvolvidas e caracterizadas as primeiras linhagens de c?lulas embrion?rias de Rhipicephalus (Boophilus) microplus oriundas de cepa brasileira. As cepas de A. marginale foram obtidas a partir de sangue de dois bezerros naturalmente infectados pertencentes ? Universidade Federal Rural do Rio de Janeiro (UFRRJ), Serop?dica. Ap?s isolamento, as cepas foram caracterizadas morfologicamente, atrav?s da microscopia de luz e molecularmente atrav?s do semi-nested PCR do gene msp1?. Duas cepas de A. marginale foram isoladas e nomeadas como AmRio1 e AmRio2. Pequenas col?nias foram observadas em l?minas de citocentr?fuga coradas com Giemsa 1:10, de cada isolado, 60 dias ap?s o in?cio da cultura. An?lises de uma repeti??o em tandem de MSP1a da cepa AmRio1 resultou em uma nova sequ?ncia (nomeada de 162) com um amino?cido diferente. O resultado da an?lise filogen?tica demonstrou que as cepas de A. marginale a partir do Brasil e Argentina formam dois grandes clusters. Para o estabelecimento das linhagens de R. (B.) microplus, os carrapatos foram provenientes de cepa mantida em bezerros no Laborat?rio de Doen?as Parasit?rias, UFRRJ, Serop?dica. No 13? dia de postura, a massa de ovos foi esterilizada, macerada, filtrada e centrifugada a 200 xg por 10 minutos. O ?pellet? foi suspenso em meio Leibovitz?s L15 e dividido em 6 frascos de 25 cm2, dos quais 3 contaminaram, um foi depositado no banco de c?lulas do Instituto Pirbright e outros 2 foram caracterizados no presente estudo (linhagens BMEBR1 e BMEBR2) atrav?s da an?lise molecular do gene 16S DNAr (PCR), curva de crescimento e microscopia de luz e epifluoresc?ncia. Com 60 dias de cultivo, as c?lulas passaram a apresentar um aspecto confluente, caracterizando a forma??o da monocamada. Existiu uma completa similaridade (100%) das novas linhagens BMEBR1 e BMEBR2 com R. (B.) microplus do Brasil (EU918178.1), da Costa Rica (EU918179.1), do Paraguai (EU918180.1), entre outros. Ambas as cepas apresentaram crescimento menor que a linhagem de R. (B.) microplus BME/CTVM2 oriunda da Costa Rica. A linhagem BMEBR1 possui maior capacidade fagoc?tica que as linhagens BMEBR2 e BME/CTVM2. Al?m disso, essas linhagens possuem caracter?sticas de acidifica??o, produ??o de corp?sculo lip?dico e estresse oxidativo diferentes entre si. As novas linhagens foram congeladas e descongeladas com sucesso e apresentaram-se est?veis com rela??o ?s caracter?sticas morfol?gicas e de taxa crescimento. Desta forma, estas linhagens podem ser utilizadas em futuros estudos na propaga??o das novas cepas de A. marginale isoladas.Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2019-06-18T15:57:31Z No. of bitstreams: 1 2015 - Bruna de Azevedo Ba?ta.pdf: 4031086 bytes, checksum: 4030be45fc06aa6f754bc07f162f640a (MD5)Made available in DSpace on 2019-06-18T15:57:31Z (GMT). No. of bitstreams: 1 2015 - Bruna de Azevedo Ba?ta.pdf: 4031086 bytes, checksum: 4030be45fc06aa6f754bc07f162f640a (MD5) Previous issue date: 2015-02-20Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico, CNPq, Brasilapplication/pdfhttps://tede.ufrrj.br/retrieve/10031/2015%20-%20Bruna%20de%20Azevedo%20Ba%c3%aata.pdf.jpghttps://tede.ufrrj.br/retrieve/16126/2015%20-%20Bruna%20de%20Azevedo%20Ba%c3%aata.pdf.jpghttps://tede.ufrrj.br/retrieve/22436/2015%20-%20Bruna%20de%20Azevedo%20Ba%c3%aata.pdf.jpghttps://tede.ufrrj.br/retrieve/28808/2015%20-%20Bruna%20de%20Azevedo%20Ba%c3%aata.pdf.jpghttps://tede.ufrrj.br/retrieve/35160/2015%20-%20Bruna%20de%20Azevedo%20Ba%c3%aata.pdf.jpghttps://tede.ufrrj.br/retrieve/41534/2015%20-%20Bruna%20de%20Azevedo%20Ba%c3%aata.pdf.jpghttps://tede.ufrrj.br/retrieve/47876/2015%20-%20Bruna%20de%20Azevedo%20Ba%c3%aata.pdf.jpghttps://tede.ufrrj.br/retrieve/54360/2015%20-%20Bruna%20de%20Azevedo%20Ba%c3%aata.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Ci?ncias Veterin?riasUFRRJBrasilInstituto de Veterin?riaABERCROMBIE, M.; HEAYSMAN, J. E. M. Observations in the social behavior of cells in tissue culture, II: ?Monolayering? of fibroblasts. Experimental Cell Research, v. 6, n. 2, p. 293-306, 1954. BA?TA, B. A. Co-cultivo de Borrelia burgdorferi (Spirochaetales: Spirochaetaceae) em cultura prim?ria de c?lulas embrion?rias de Dermacentor nitens (Acari: Ixodidae). 2011. 39f. Disserta??o (Mestrado em Ci?ncias), Universidade Federal Rural do Rio de Janeiro, Serop?dica, Rio de Janeiro. BARROS-BATTESTI, D. M.; ARZUA, M.; BECHARA, G. H. Carrapatos de Import?ncia M?dico-Veterin?ria da Regi?o Neotropical: Um guia ilustrado para identifica??o de esp?cies, Vox/ICTTD-3/Butantan, 2006, 223 p. BEATI, L.; KEIRANS, J. E. Analysis of the systematic relationships among ticks of the genera Rhipicephalus and Boophilus (Acari: Ixodidae) based on mitochondrial 12S ribosomal DNA gene sequences and morphological characters. Journal of Parasitology, v. 87, n. 1, p. 32?48, 2001. BELL-SAKYI, L. Ehrlichia ruminantium Grows in Cell Lines from Four Ixodid Tick Genera. Journal of Comparative Pathology, v. 130, n. 4, p. 285?293, 2004. BELL-SAKYI, L.; PAXTON, E. A.; MUNDERLOH, U. G.; SUMPTION, K. J. Growth of Cowdria ruminantium, the causative agent of heartwater, in a tick line. Journal of Clinical Microbiology v. 38, n. 3, p. 1238-1240, 2000. BELL-SAKYI, L.; ZWEYGARTH, E.; BLOUIN, E. F.; GOULD, E. A.; JONGEJAN, F. Tick cell lines: tools for tick and tick-borne disease research. Trends in Parasitology, v. 23, n. 9, p. 450-457, 2007. BHAT, U. K. M.; MAHONEY, D. F.; WRIGHT, I. G. The invasion and growth of Babesia bovis in tick tissue culture. Experientia, v. 35, n. 6, p.752-753, 1979. BHAT, U. K. M.; YUNKER, C. E. Establishment and characterization of a diploid cell line from the tick, Dermacentor parumapertus Neumann (Acarina: Ixodidae). The Journal of Parasitology, v. 63, n. 6, p. 1092-1098, 1977. BOZZA, P. T.; MAGALH?ES, K. G.; WELLER, P. F. Leukocyte lipid bodies ? biogenesis and functions in inflammation. Biochimica et Biophysica Acta, v. 1791, n. 6, p. 540-551, 2009. CARREL, A. On the permanent life of tissues outside the organism. Journal of experimental medicine, v.15, n. 5, p. 516-528, 1912. CDC - Centers for Disease Control and Prevention. One-Host Ixodid Tick Life Cycle. Dispon?vel em: <http://www.cdc.gov/dpdx/ticks/> Acesso em: 01 ago. 2014. 73 D?AVILA, H.; FREIRE-DE-LIMA, C. G.; ROQUE, N. R.; TEIXEIRA, L.; BARJAFIDALGO, C.; SILVA, A. R.; MELO, R. C.; DOSREIS, G. A.; CASTRO-FARIANETO, H. C.; BOZZA, P. T. Host cell lipid bodies triggered by Trypanosoma cruzi infection and enhanced by the uptake of apoptotic cells are associated with prostaglandin E2 generation and increased parasite growth. The Journal of Infectious Diseases, v. 204, n. 6, p. 951-961, 2011. DE LA FUENTE, J.; BLOUIN, E. F.; MANZANO-ROMAN, R.; NARANJO, V.; ALMAZAN, C.; PEREZ DE LA LASTRA, J. M.; ZIVKOVIC, Z.; JONGEJAN, F.; KOCAN, K. M. Functional genomic studies of tick cells in response to infection with the cattle pathogen Anaplasma marginale. Genomics. v. 90, n. 6, p. 712?722, 2007. DULBECCO, R.; ELKINGTON, J. Conditions limiting multiplication of fibroblastic and epithelial cells in dense cultures. Nature, v. 246, n. 1, p.197-199, 1973. EIDE, P. E.; CALDWELL, J. M. A method for obtaining primary cultures of dispersed embryonic tissue from the lone star tick, Amblyomma americanum. Annals of the Entomological Society of America, v.66, n. 1, p. 891-893, 1973. ESTEVES, E.; BASTOS, C. V.; ZIVKOVIC, Z.; DE LA FUENTE, J.; KOCAN, K.; BLOUIN, E.; RIBEIRO, M. F. B.; PASSOS, L. M. F.; DAFFRE, S. Propagation of a Brazilian isolate of Anaplasma marginale with appendage in a tick cell line (BME/CTVM26) derived from Rhipicephalus (Boophilus) microplus. Veterinary Parasitology, v. 161, n. 1, p. 150?153, 2009. ESTEVES, E.; LARA, F. A.; LORENZINI, D. M.; COSTA, G. H.; FUKUZAWA, A. H.; PRESSINOTTI, L. N.; SILVA, J. R.; FERRO, J. A.; KURTTI, T. J.; MUNDERLOH, U. G.; DAFFRE, S. Cellular and molecular characterization of an embryonic cell line (BME/CTVM26) from the tick Rhipicephalus (Boophilus) microplus. Insect Biochemistry and Molecular Biology, v. 38, n. 5, p. 568?580, 2008. EWING, S. A., MUNDERLOH, U. G., BLOUIN, E. F., KOCAN, K. M.; KURTTI, T. J. 1995. Ehrlichia canis in tick cell culture. Proceedings of the 76th Conference of Research Workers in Animal Diseases, Chicago, USA, 13?14 November 1995. Ames: Iowa State University Press (Abstract no. 165). FLECHTMANN, C.A.W. ?caros de import?ncia m?dico veterin?ria. 3.ed. S?o Paulo: Nobel, 1990. 192 p. FOLKMAN, I.; MOSCONA, A. Role of cell shape in growth control. Nature, v. 273, n. 1, p. 345-349, 1978. FRESHNEY, R. I. Culture of Animal Cells: A manual of basic technique. 5? Ed. New Jersey: John Wiley and Sons, 2005, 642 p. GONZ?LES, J. C. O carrapato dos bovinos Boophilus microplus (Canestrini, 1887) (Revis?o hist?rica e concentual). A Hora Veterin?ria, v. 21, n. 1, p. 23-28 2002. 74 GRISI, L.; LEITE, R. C.; MARTINS, J. R. S.; BARROS, A. T. M.; ANDREOTTI, R.; CAN?ADO, P. H. D.; LE?N, A. A. P.; PEREIRA, J. B.; VILLELA, H. S. Reassessment of the potential economic impact of cattle parasites in Brazil. Revista Brasileira de Parasitologia Veterin?ria, v. 23, n. 2, p. 150-156, 2014. HARRISON, R. G. Observations on the living developing nerve fiber. Journal of Experimental Zoology Proceedings of the Society for Experimental Biology and Medicine, v.4, n. 1, p. 140-143, 1907. HAY, R. J.; MIRANDA-CLELAND, M.; DURKIN, S.; REID, Y. A. Cell line preservation and authentication. In: MASTERS, J. R. W. Animal cell culture. Ed., Oxford: Oxford University Press, 2000, p. 69-103. HOLMAN, P. J. Partial characterization of a unique female diploid cell strain from the tick Boophilus microplus (Acari: Ixodidae). Journal of Medical Entomology, v. 18, n. 1, p. 84-88, 1981. HOLMAN, P. J.; RONALD, N. C. A new tick cell line derived from Boophilus microplus. Research in Veterinary Science, v. 29, n. 3, p. 383-387, 1980. KURTTI, T. J.; B?SCHER, G. Trends in tick cell culture. In: MARAMOROSCH, K.; HIRUMI, H. Practical Tissue Culture Applications, New York: Academic Press, 1979, p. 351-371. KURTTI, T. J.; MUNDERLOH, U. G. Tick cell culture: Characteristics, growth requeriments, and applications to parasitology. In: MARAMOROSCH, K.; MITSUHASHI, J. Invertebrate cell culture Applications, New York: Academic Press, 1982, p. 195-232. KURTTI, T. J.; MUNDERLOH, U. G.; AHLSTRAND, G. G. Tick tissue and cell culture in vector research. In: HARRIS, K. F. Advances in disease vector research, v. 5, New York: Springer-Verlag, 1988a, p. 87-109. KURTTI, T. J.; MUNDERLOH, U. G.; AHLSTRAND, G. G.; JOHNSON, R. C. Borrelia burgdorferi in Tick Cell Culture: Growth and Cellular Adherence. Journal of Medical Entomology, v. 25, n. 4, p. 256-261, 1988b. KURTTI, T. J.; MUNDERLOH, U. G.; KRUEGER, D. E.; JOHNSON, R. C.; SCHWAN, T. G. Adhesion to and Invasion of Cultured Tick (Acarina: Ixodidae) Cells by Borrelia burgdorferi (Spirochaetales: Spirochaetaceae) and Maintenance of Infectivity. Journal of Medical Entomology, v. 30, n. 3, p. 586-596, 1993. KURTTI, T. J.; MUNDERLOH, U. G.; SAMISH, M. Effect of medium supplements on tick cells in culture. Journal of Parasitology, v. 68, n. 5, p. 930-935, 1982. KURTTI, T. J., SIMSER, J. A., BALDRIDGE, G. D., PALMER, A. T., & MUNDERLOH, U. G. Factors influencing in vitro infectivity and growth of Rickettsia peacockii (Rickettsiales: Rickettsiaceae), an endosymbiont of the Rocky Mountain 75 wood tick, Dermacentor andersoni (Acari, Ixodidae). Journal of invertebrate pathology, v. 30, n. 3, p. 177-186, 2005. KURTTI, T.J., MUNDERLOH, U.G., STILLER, D. The interaction of Babesia caballi kinetes with tick cells. Journal of Invertebrate Pathology v. 42, n. 3, p. 334?343, 1983. MATTILA, J. T.; MUNDERLOH, U. G.; KURTTI, T. J. Phagocytosis of the Lyme disease spirochete, Borrelia burgdorferi, by cells from the ticks, Ixodes scapularis and Dermacentor andersoni, infected with an endosymbiont, Rickettsia peacockii. Journal of insect science (Online). v. 7, n. 58, p. 1-12, 2007. MATTOS, K. A.; LARA, F. A.; OLIVEIRA, V. G.; RODRIGUES, L. S.; D'AVILA, H.; MELO, R. C.; MANSO, P. P; SARNO, E. N.; BOZZA, P. T.; PESSOLANI, M. C. Modulation of lipid droplets by Mycobacterium leprae in Schwann cells: a putative mechanism for host lipid acquisition and bacterial survival in phagosomes. Cellular Microbiology. v. 13, n. 2, p. 259?273, 2011. MARTIN, H. M.; VIDLER, B. O. In vitro growth of tick tissues Rhipicephalus appendiculatus (Neumann, 1901). Experimental Parasitology, v. 12, n. 1, p. 192-203, 1962. MELO, R. C. N.; DVORAK, A. M. Lipid body-phagosome interaction in macrophages during infectious diseases: host defense or pathogen survival strategy?. PLOS Pathogens, v. 8, n. 7, 2012. MELO, R. C. N.; D?AVILA, H.; WAN, H. C.; BOZZA, P. T.; DVORAK, A. M.; WELLER, P. F. Lipid bodies in inflammatory cells: structure, function, and current imaging techniques. Journal of Histochemistry & Cytochemistry, v. 59, n. 5, p. 540- 556, 2011a. LALLINGER, G.; ZWEYGARTH, E.; BELL-SAKYI, L.; PASSOS, L. M. Cold storage and cryopreservation of tick cell lines. Parasites & Vectors, v. 3, n. 37, p. 1-6, 2010. LEIBO, S. P. ; MAZUR, P. The role of cooling rates in low-temperature preservation. Cryobiology, v. 8, n. 5, p. 447-452, 1971. MANGOLD, A. J.; BARGUES, M. D.; MAS-COMA, S. Mitochondrial 16S rDNA sequences and phylogenetic relationships of species of Rhipicephalus and other tick genera among Metastriata (Acari: Ixodidae). Parasitology Research, v. 84, n. 6, p. 478- 484, 1998. MEDVEDEVA, G. I.; BESKINA, S. R.; GROKHOVSKAYA, I. M. Culture of ixodid tick embryonic cells. Medical Parasitology, v. 41, n. 1, p. 39-40, 1972. MOSQUEDA, J. et al. Primary Midgut, Salivary Gland, and Ovary Cultures from Boophilus microplus. Annals of the New York Academy of Sciences, v. 1149, n. 1, p. 49, 2008. 76 MUNDERLOH, U. G.; BLOUIN, E. F.; KOCAN, K. M.; GE, N. L.; EDWARDS, W. L.; KURTTI, T. J. Establishment of the tick (Acari: Ixodidae) borne cattle pathogen Anaplasma marginale (Rickettsiales: Anaplasmataceae) in tick cell culture. Journal of Medical Entomology, v. 33, n. 4, p. 656-664, 1996. MUNDERLOH, U. G.; JAURON, S. D.; FINGERLE, V.; LEITRITZ, L.; HAYES, S. F.; HAUTMAN, J. M.; NELSON, C. M.; HUBERTY, B. W.; KURTTI, T. J.; AHLSTRAND, G. G.; GREIG, B.; MELLEMCAMP, M. A.; GOODMAN, J. L. Invasion an intracellular development of the human granulocytic ehrlichiosis agent in tick cell culture. Journal of Clinical Microbiology, v. 37, n. 8, p. 2518-2524, 1999. MUNDERLOH, U. G.; KURTTI, T. J. Malarial parasites complete sporogony in axenic mosquitoes. Experientia, v. 41, n. 9, p. 1205-1207, 1985. MUNDERLOH, U. G.; KURTTI, T. J. Formulation of medium for tick cell culture. Experimental & Applied Acarology, v. 7, n. 3, p. 219-229, 1989. MUNDERLOH, U. G.; KURTTI, T. J. Cellular and molecular interrelationships between tick-borne pathogens. Annual Reviews Entomology, v. 40, n. 1, p. 221-243, 1995. MUNDERLOH, U. G.; LIU, Y.; WANG, M.; CHEN, C.; KURTTI, T. J. Establishment, Maintenance and Description of Cell Lines from the Tick Ixodes scapularis. The Journal of Parasitology, v. 80, n. 4, p. 533-543, 1994. MURGIA, R.; CINCO, M. Induction of cystic forms by different stress conditions in Borrelia burgdorferi. APMIS v. 112, n. 1, p. 57?62, 2004. MURRELL, A.; BARKER, S. C. Synonymy of Boophilus Curtice, 1891 with Rhipicephalus Koch, 1844 (Acari: Ixodidae). Systematic Parasitology, v. 56, n. 3, p. 169-172, 2003. NUNES, E. T.; BECHARA, G. H.; SAITO, K. C.; DENARDI, S. E.; OLIVEIRA, P. R.; MATHIAS, M. I. C. Morphological, histological, and ultrastructural characterization of degenerating salivary glands in females of the cattle-tick Rhipicephalus (Boophilus) microplus (CANESTRINI, 1887)(Acari: Ixodidae). Micron, v. 36, n. 5, p. 437-447, 2005. NU?ES, J. L.; MU?OZ COBENAS, M. E.; MOLTEDO, H. L. Boophilus microplus, la garrapata comum del ganado vacuno. Buenos Aires: Hemisf?rio Sur, 1982. 19 p. PASSOS, L. M. F. In vitro cultivation of Anaplasma marginale and A. phagocytophilum in tick cell lines: a review. Revista Brasileira De Parasitologia Veterinaria, v. 21, n. 2, p. 81-86, 2012. PUDNEY, M.; VARMA, M. G. R.; LEAKE, C. J. Culture of embryonic cells from the tick Boophilus microplus (Ixodidae). Journal of Medical Entomology, v. 10, n. 5, p. 493-496, 1973. 77 ?EH??EK, J; BRZOSTOWSKI, H. W. A tick tissue culture medium based on analyses of tick haemolymph. Journal of Insect Physiology, v. 15, n. 8, p. 1431-1436, 1969. REZENDE, J. Cultura prim?ria in vitro de c?lulas embrion?rias de Rhipicephalus (Boophilus) microplus e Amblyomma cajennense como substrato para cultivo de Borrelia burgdorferi. 2008. 22f. Disserta??o (Mestrado em Ci?ncias), Universidade Federal Rural do Rio de Janeiro, Serop?dica, Rio de Janeiro. RIBEIRO, M. F.; BASTOS, C. V.; VASCONCELOS, M. M. C.; PASSOS, L. M. F. < Babesia bigemina: In vitro multiplication of sporokinetes in Ixodes scapularis (IDE8) cells. Experimental parasitology. v. 122, n. 3, p. 192-195, 2009. RITTIG, M. G.; KUHN, K. H.; DECHANT, C. A.; GAUCKLER, A.; MODOLELL, M.; RICCIARDI-CASTAGNOLI, P.; KRAUSE, A.; BURMESTER, G. R. Phagocytes from both vertebrate and invertebrate species use "coiling" phagocytosis. Developmental and Comparative Immunology, v. 20, n. 6, p. 393-406, 1996. RUDENKO, N.; GOLOVCHENKO, M.; NEMEC, J.; VOLKAERT, J.; MALLATOVA, N.; GRUBHOFFER, L. Improved method of detection and molecular typing of Borrelia burgdorferi sensu lato in clinical samples by polymerase chain reaction without DNA purification. Folia Microbiology. v. 50, n. 1, p. 31?39, 2005. SILVA, P. P. An?lise dos corp?sculos lip?dicos em macr?fagos humanos infectados pela cepa Y do Trypanosoma cruzi e submetidos ao tratamento com a droga benzonidazol, 2012, 91f., Disserta??o (Mestrado em Biologia Celular), Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais. STOKER, M. G. P. Role of diffusion boundary layer in contact inhibition of growth. Nature, v. 246, n. 5430, p. 200-203, 1973. TEIXEIRA, R. C. Cultivo de Borrelia burgdorferi (Spirochaetales: Spirochaetaceae) em c?lulas embrion?rias de Rhipicephalus sanguineus (Acari: Ixodidae). 2010. 28f. Disserta??o (Mestrado em Ci?ncias), Universidade Federal Rural do Rio de Janeiro, Serop?dica, Rio de Janeiro. TEIXEIRA, R. C. Cultivo de Borrelia burgdorferi e Borrelia anserina (Spirochaetales: Spirochaetaceae) em diferentes linhagens celulares de carrapatos (Acari: Ixodidae). 2014. 71f. Tese (Doutorado em Ci?ncias), Universidade Federal Rural do Rio de Janeiro, Serop?dica, Rio de Janeiro. VARMA, M. G. R.; PUDNEY, M.; LEAKY, C. J. The establishment of three cell lines from the tick Rhipicephalus appendiculatus (Acari: Ixodidae) and their infection with some arbovirusis. Journal of Medical Entomology, v. 11, n. 6, p. 698-706, 1975. WHARTON, R. H. Ticks with special emphasis on Boophilus microplus. In: PAL, R.; WHARTON, R. H. Control of arthropods of medical and veterinary importance. London Plenum Press, 1974, p. 35-52. 78 WEYER, F. Explantationsversuche bei L?usen in Verbindung mit der Kultur von Rickettsien. Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene, v. 159, n. 1-2, p. 13-22, 1952. WU, J.; WEENING, E. H.; FASKE, J. B.; H??K, M.; SKARE, J. T. Invasion of eukaryotic cells by Borrelia burgdorferi requires ?1 integrins and Src kinase activity. Infection and Immunity, v. 79, n. 3, p. 338?1348, 2011. YUNKER, C. E. Preparation and maintenance of arthropod cell cultures: Acari, with emphasis on ticks. In: YUNKER, C. E. Arboviruses in arthropod cells in vitro. Boca Raton: CRC Press, 1987. p. 35-51. YUNKER, C. E.; CORY, J. Effectiveness of refrigerated nymphs in tick tissue culture experiments. Journal of Parasitology, v. 51, n. 4, p. 686, 1965.cultivo in vitroAnaplasma marginaleRhipicephalus (Boophilus) microplusin vitro cultureAnaplasma marginaleRhipicephalus (Boophilus) microplusMedicina Veterin?riaIsolamento e caracteriza??o molecular de duas novas cepas de Anaplasma marginale e estabelecimento de linhagens de c?lulas embrion?rias do carrapato Rhipicephalus (Boophilus) microplusIsolation and molecular characterization of two new strains of A. marginale and establishment of embryonic cell lines from the tick Rhipicephalus (Boophilus) microplus.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRRJinstname:Universidade Federal Rural do Rio de Janeiro (UFRRJ)instacron:UFRRJTHUMBNAIL2015 - Bruna de Azevedo Ba?ta.pdf.jpg2015 - Bruna de Azevedo Ba?ta.pdf.jpgimage/jpeg1943http://localhost:8080/tede/bitstream/jspui/2753/18/2015+-+Bruna+de+Azevedo+Ba%C3%AAta.pdf.jpgcc73c4c239a4c332d642ba1e7c7a9fb2MD518TEXT2015 - Bruna de Azevedo Ba?ta.pdf.txt2015 - Bruna de Azevedo Ba?ta.pdf.txttext/plain189617http://localhost:8080/tede/bitstream/jspui/2753/17/2015+-+Bruna+de+Azevedo+Ba%C3%AAta.pdf.txt643c88711930e9fb9b6cf1a3fa780839MD517ORIGINAL2015 - Bruna de Azevedo Ba?ta.pdf2015 - Bruna de Azevedo Ba?ta.pdfapplication/pdf4031086http://localhost:8080/tede/bitstream/jspui/2753/2/2015+-+Bruna+de+Azevedo+Ba%C3%AAta.pdf4030be45fc06aa6f754bc07f162f640aMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82089http://localhost:8080/tede/bitstream/jspui/2753/1/license.txt7b5ba3d2445355f386edab96125d42b7MD51jspui/27532022-05-30 10:51:12.697oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttps://tede.ufrrj.br/PUBhttps://tede.ufrrj.br/oai/requestbibliot@ufrrj.br||bibliot@ufrrj.bropendoar:2022-05-30T13:51:12Biblioteca Digital de Teses e Dissertações da UFRRJ - Universidade Federal Rural do Rio de Janeiro (UFRRJ)false
dc.title.por.fl_str_mv Isolamento e caracteriza??o molecular de duas novas cepas de Anaplasma marginale e estabelecimento de linhagens de c?lulas embrion?rias do carrapato Rhipicephalus (Boophilus) microplus
dc.title.alternative.eng.fl_str_mv Isolation and molecular characterization of two new strains of A. marginale and establishment of embryonic cell lines from the tick Rhipicephalus (Boophilus) microplus.
title Isolamento e caracteriza??o molecular de duas novas cepas de Anaplasma marginale e estabelecimento de linhagens de c?lulas embrion?rias do carrapato Rhipicephalus (Boophilus) microplus
spellingShingle Isolamento e caracteriza??o molecular de duas novas cepas de Anaplasma marginale e estabelecimento de linhagens de c?lulas embrion?rias do carrapato Rhipicephalus (Boophilus) microplus
Ba?ta, Bruna de Azevedo
cultivo in vitro
Anaplasma marginale
Rhipicephalus (Boophilus) microplus
in vitro culture
Anaplasma marginale
Rhipicephalus (Boophilus) microplus
Medicina Veterin?ria
title_short Isolamento e caracteriza??o molecular de duas novas cepas de Anaplasma marginale e estabelecimento de linhagens de c?lulas embrion?rias do carrapato Rhipicephalus (Boophilus) microplus
title_full Isolamento e caracteriza??o molecular de duas novas cepas de Anaplasma marginale e estabelecimento de linhagens de c?lulas embrion?rias do carrapato Rhipicephalus (Boophilus) microplus
title_fullStr Isolamento e caracteriza??o molecular de duas novas cepas de Anaplasma marginale e estabelecimento de linhagens de c?lulas embrion?rias do carrapato Rhipicephalus (Boophilus) microplus
title_full_unstemmed Isolamento e caracteriza??o molecular de duas novas cepas de Anaplasma marginale e estabelecimento de linhagens de c?lulas embrion?rias do carrapato Rhipicephalus (Boophilus) microplus
title_sort Isolamento e caracteriza??o molecular de duas novas cepas de Anaplasma marginale e estabelecimento de linhagens de c?lulas embrion?rias do carrapato Rhipicephalus (Boophilus) microplus
author Ba?ta, Bruna de Azevedo
author_facet Ba?ta, Bruna de Azevedo
author_role author
dc.contributor.advisor1.fl_str_mv Fonseca, Adivaldo Henrique da
dc.contributor.advisor1ID.fl_str_mv 475.018.557-49
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/4411441162862608
dc.contributor.advisor-co1.fl_str_mv Zweygarth, Erich
dc.contributor.referee1.fl_str_mv Piranda, Eliane Mattos
dc.contributor.referee2.fl_str_mv Decker, Lygia Maria
dc.contributor.referee3.fl_str_mv Baldani, Cristiane Divan
dc.contributor.referee4.fl_str_mv Santos, Huarrisson Azevedo
dc.contributor.authorID.fl_str_mv 101.354.697-08
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/8016012971743463
dc.contributor.author.fl_str_mv Ba?ta, Bruna de Azevedo
contributor_str_mv Fonseca, Adivaldo Henrique da
Zweygarth, Erich
Piranda, Eliane Mattos
Decker, Lygia Maria
Baldani, Cristiane Divan
Santos, Huarrisson Azevedo
dc.subject.por.fl_str_mv cultivo in vitro
Anaplasma marginale
Rhipicephalus (Boophilus) microplus
topic cultivo in vitro
Anaplasma marginale
Rhipicephalus (Boophilus) microplus
in vitro culture
Anaplasma marginale
Rhipicephalus (Boophilus) microplus
Medicina Veterin?ria
dc.subject.eng.fl_str_mv in vitro culture
Anaplasma marginale
Rhipicephalus (Boophilus) microplus
dc.subject.cnpq.fl_str_mv Medicina Veterin?ria
description In the first chapter two new strains of Anaplasma marginale derived from the Serop?dica, RJ were isolated and characterized in tick cells line of Ixodes scapularis (IDE8), enabling new studies in the diagnosis with the use of local strains. In the second chapter the first lines of embryonic cells of Rhipicephalus (Boophilus) microplus from Brazilian strains were developed and characterized. The strains of A. marginale were from blood of two naturally infected calves belonging to the Universidade Federal Rural do Rio de Janeiro (UFRRJ), Serop?dica. After isolation, the strains were characterized morphologically by light microscopy and molecularly by nested PCR of msp1? gene. Two strains of A. marginale were isolated and named as AmRio1 and AmRio2. Small colonies were observed in cytospin smears stained with Giemsa?s 1:10, of each of the isolates, 60 days after culture initiation. Analysis of msp1? microsatellite in both strains resulted in a single genotype, genotype E. The amino acid sequence of one MSP1? tandem repeat from the strain AmRio1 resulted in a new sequence (named 162) with one amino acid change. The results of our phylogenetic analysis demonstrated that A. marginale strains from Brazil and Argentina formed two large clusters. In establishing of the strains of R. (B.) microplus, the ticks were from strain kept calves in Parasitic Diseases Laboratory, UFRRJ, Serop?dica. After 13 days of laying, the pool of eggs was sterilized, macerated, filtered and centrifuged at 200 xg for 10 minutes. The pellet was suspended in Leibotiz?s L15 medium and divided into 6 flasks of 25 cm2, of which 3 were contaminated, one was deposited in the cell bank of the Pirbright Institute and others two were characterized in the present study (BMEBR1 and BMEBR2 lines) through molecular analysis of the 16S rDNA gene (PCR), growth curve and epifluorescence and light microscopy. Sixty days in culture, the cells began to show confluent aspect, reaching the formation of the monolayer. There was a complete similarity (100%) of new lines BMEBR1 and BMEBR2 with R. (B.) microplus in Brazil (EU918178.1), Costa Rica (EU918179.1), Paraguay (EU918180.1), among others. Both strains showed lower growth than the R. (B.) microplus line from Costa Rica, BME/CTVM2. The BMEBR1 line has increased phagocytic ability that the BMEBR2 and BME/CTVM2 lines. In addition, these strains have acidification characteristics, lipid corpuscle production and oxidative stress different from each other. The new strains were frozen and defrosted successfully, and were stable with respect to morphology and growth rate. Thus, these lines can be used in future studies to propagation of new strains of A. marginale isolated.
publishDate 2015
dc.date.issued.fl_str_mv 2015-02-20
dc.date.accessioned.fl_str_mv 2019-06-18T15:57:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv Ba?ta, Bruna de Azevedo. Isolamento e caracteriza??o molecular de duas novas cepas de Anaplasma marginale e estabelecimento de linhagens de c?lulas embrion?rias do carrapato Rhipicephalus (Boophilus) microplus. 2015. [98 f.]. Tese( Programa de P?s-Gradua??o em Ci?ncias Veterin?rias) - Universidade Federal Rural do Rio de Janeiro, [Serop?dica - Rio de Janeiro] .
dc.identifier.uri.fl_str_mv https://tede.ufrrj.br/jspui/handle/jspui/2753
identifier_str_mv Ba?ta, Bruna de Azevedo. Isolamento e caracteriza??o molecular de duas novas cepas de Anaplasma marginale e estabelecimento de linhagens de c?lulas embrion?rias do carrapato Rhipicephalus (Boophilus) microplus. 2015. [98 f.]. Tese( Programa de P?s-Gradua??o em Ci?ncias Veterin?rias) - Universidade Federal Rural do Rio de Janeiro, [Serop?dica - Rio de Janeiro] .
url https://tede.ufrrj.br/jspui/handle/jspui/2753
dc.language.iso.fl_str_mv por
language por
dc.relation.references.por.fl_str_mv ABERCROMBIE, M.; HEAYSMAN, J. E. M. Observations in the social behavior of cells in tissue culture, II: ?Monolayering? of fibroblasts. Experimental Cell Research, v. 6, n. 2, p. 293-306, 1954. BA?TA, B. A. Co-cultivo de Borrelia burgdorferi (Spirochaetales: Spirochaetaceae) em cultura prim?ria de c?lulas embrion?rias de Dermacentor nitens (Acari: Ixodidae). 2011. 39f. Disserta??o (Mestrado em Ci?ncias), Universidade Federal Rural do Rio de Janeiro, Serop?dica, Rio de Janeiro. BARROS-BATTESTI, D. M.; ARZUA, M.; BECHARA, G. H. Carrapatos de Import?ncia M?dico-Veterin?ria da Regi?o Neotropical: Um guia ilustrado para identifica??o de esp?cies, Vox/ICTTD-3/Butantan, 2006, 223 p. BEATI, L.; KEIRANS, J. E. Analysis of the systematic relationships among ticks of the genera Rhipicephalus and Boophilus (Acari: Ixodidae) based on mitochondrial 12S ribosomal DNA gene sequences and morphological characters. Journal of Parasitology, v. 87, n. 1, p. 32?48, 2001. BELL-SAKYI, L. Ehrlichia ruminantium Grows in Cell Lines from Four Ixodid Tick Genera. Journal of Comparative Pathology, v. 130, n. 4, p. 285?293, 2004. BELL-SAKYI, L.; PAXTON, E. A.; MUNDERLOH, U. G.; SUMPTION, K. J. Growth of Cowdria ruminantium, the causative agent of heartwater, in a tick line. Journal of Clinical Microbiology v. 38, n. 3, p. 1238-1240, 2000. BELL-SAKYI, L.; ZWEYGARTH, E.; BLOUIN, E. F.; GOULD, E. A.; JONGEJAN, F. Tick cell lines: tools for tick and tick-borne disease research. Trends in Parasitology, v. 23, n. 9, p. 450-457, 2007. BHAT, U. K. M.; MAHONEY, D. F.; WRIGHT, I. G. The invasion and growth of Babesia bovis in tick tissue culture. Experientia, v. 35, n. 6, p.752-753, 1979. BHAT, U. K. M.; YUNKER, C. E. Establishment and characterization of a diploid cell line from the tick, Dermacentor parumapertus Neumann (Acarina: Ixodidae). The Journal of Parasitology, v. 63, n. 6, p. 1092-1098, 1977. BOZZA, P. T.; MAGALH?ES, K. G.; WELLER, P. F. Leukocyte lipid bodies ? biogenesis and functions in inflammation. Biochimica et Biophysica Acta, v. 1791, n. 6, p. 540-551, 2009. CARREL, A. On the permanent life of tissues outside the organism. Journal of experimental medicine, v.15, n. 5, p. 516-528, 1912. CDC - Centers for Disease Control and Prevention. One-Host Ixodid Tick Life Cycle. Dispon?vel em: <http://www.cdc.gov/dpdx/ticks/> Acesso em: 01 ago. 2014. 73 D?AVILA, H.; FREIRE-DE-LIMA, C. G.; ROQUE, N. R.; TEIXEIRA, L.; BARJAFIDALGO, C.; SILVA, A. R.; MELO, R. C.; DOSREIS, G. A.; CASTRO-FARIANETO, H. C.; BOZZA, P. T. Host cell lipid bodies triggered by Trypanosoma cruzi infection and enhanced by the uptake of apoptotic cells are associated with prostaglandin E2 generation and increased parasite growth. The Journal of Infectious Diseases, v. 204, n. 6, p. 951-961, 2011. DE LA FUENTE, J.; BLOUIN, E. F.; MANZANO-ROMAN, R.; NARANJO, V.; ALMAZAN, C.; PEREZ DE LA LASTRA, J. M.; ZIVKOVIC, Z.; JONGEJAN, F.; KOCAN, K. M. Functional genomic studies of tick cells in response to infection with the cattle pathogen Anaplasma marginale. Genomics. v. 90, n. 6, p. 712?722, 2007. DULBECCO, R.; ELKINGTON, J. Conditions limiting multiplication of fibroblastic and epithelial cells in dense cultures. Nature, v. 246, n. 1, p.197-199, 1973. EIDE, P. E.; CALDWELL, J. M. A method for obtaining primary cultures of dispersed embryonic tissue from the lone star tick, Amblyomma americanum. Annals of the Entomological Society of America, v.66, n. 1, p. 891-893, 1973. ESTEVES, E.; BASTOS, C. V.; ZIVKOVIC, Z.; DE LA FUENTE, J.; KOCAN, K.; BLOUIN, E.; RIBEIRO, M. F. B.; PASSOS, L. M. F.; DAFFRE, S. Propagation of a Brazilian isolate of Anaplasma marginale with appendage in a tick cell line (BME/CTVM26) derived from Rhipicephalus (Boophilus) microplus. Veterinary Parasitology, v. 161, n. 1, p. 150?153, 2009. ESTEVES, E.; LARA, F. A.; LORENZINI, D. M.; COSTA, G. H.; FUKUZAWA, A. H.; PRESSINOTTI, L. N.; SILVA, J. R.; FERRO, J. A.; KURTTI, T. J.; MUNDERLOH, U. G.; DAFFRE, S. Cellular and molecular characterization of an embryonic cell line (BME/CTVM26) from the tick Rhipicephalus (Boophilus) microplus. Insect Biochemistry and Molecular Biology, v. 38, n. 5, p. 568?580, 2008. EWING, S. A., MUNDERLOH, U. G., BLOUIN, E. F., KOCAN, K. M.; KURTTI, T. J. 1995. Ehrlichia canis in tick cell culture. Proceedings of the 76th Conference of Research Workers in Animal Diseases, Chicago, USA, 13?14 November 1995. Ames: Iowa State University Press (Abstract no. 165). FLECHTMANN, C.A.W. ?caros de import?ncia m?dico veterin?ria. 3.ed. S?o Paulo: Nobel, 1990. 192 p. FOLKMAN, I.; MOSCONA, A. Role of cell shape in growth control. Nature, v. 273, n. 1, p. 345-349, 1978. FRESHNEY, R. I. Culture of Animal Cells: A manual of basic technique. 5? Ed. New Jersey: John Wiley and Sons, 2005, 642 p. GONZ?LES, J. C. O carrapato dos bovinos Boophilus microplus (Canestrini, 1887) (Revis?o hist?rica e concentual). A Hora Veterin?ria, v. 21, n. 1, p. 23-28 2002. 74 GRISI, L.; LEITE, R. C.; MARTINS, J. R. S.; BARROS, A. T. M.; ANDREOTTI, R.; CAN?ADO, P. H. D.; LE?N, A. A. P.; PEREIRA, J. B.; VILLELA, H. S. Reassessment of the potential economic impact of cattle parasites in Brazil. Revista Brasileira de Parasitologia Veterin?ria, v. 23, n. 2, p. 150-156, 2014. HARRISON, R. G. Observations on the living developing nerve fiber. Journal of Experimental Zoology Proceedings of the Society for Experimental Biology and Medicine, v.4, n. 1, p. 140-143, 1907. HAY, R. J.; MIRANDA-CLELAND, M.; DURKIN, S.; REID, Y. A. Cell line preservation and authentication. In: MASTERS, J. R. W. Animal cell culture. Ed., Oxford: Oxford University Press, 2000, p. 69-103. HOLMAN, P. J. Partial characterization of a unique female diploid cell strain from the tick Boophilus microplus (Acari: Ixodidae). Journal of Medical Entomology, v. 18, n. 1, p. 84-88, 1981. HOLMAN, P. J.; RONALD, N. C. A new tick cell line derived from Boophilus microplus. Research in Veterinary Science, v. 29, n. 3, p. 383-387, 1980. KURTTI, T. J.; B?SCHER, G. Trends in tick cell culture. In: MARAMOROSCH, K.; HIRUMI, H. Practical Tissue Culture Applications, New York: Academic Press, 1979, p. 351-371. KURTTI, T. J.; MUNDERLOH, U. G. Tick cell culture: Characteristics, growth requeriments, and applications to parasitology. In: MARAMOROSCH, K.; MITSUHASHI, J. Invertebrate cell culture Applications, New York: Academic Press, 1982, p. 195-232. KURTTI, T. J.; MUNDERLOH, U. G.; AHLSTRAND, G. G. Tick tissue and cell culture in vector research. In: HARRIS, K. F. Advances in disease vector research, v. 5, New York: Springer-Verlag, 1988a, p. 87-109. KURTTI, T. J.; MUNDERLOH, U. G.; AHLSTRAND, G. G.; JOHNSON, R. C. Borrelia burgdorferi in Tick Cell Culture: Growth and Cellular Adherence. Journal of Medical Entomology, v. 25, n. 4, p. 256-261, 1988b. KURTTI, T. J.; MUNDERLOH, U. G.; KRUEGER, D. E.; JOHNSON, R. C.; SCHWAN, T. G. Adhesion to and Invasion of Cultured Tick (Acarina: Ixodidae) Cells by Borrelia burgdorferi (Spirochaetales: Spirochaetaceae) and Maintenance of Infectivity. Journal of Medical Entomology, v. 30, n. 3, p. 586-596, 1993. KURTTI, T. J.; MUNDERLOH, U. G.; SAMISH, M. Effect of medium supplements on tick cells in culture. Journal of Parasitology, v. 68, n. 5, p. 930-935, 1982. KURTTI, T. J., SIMSER, J. A., BALDRIDGE, G. D., PALMER, A. T., & MUNDERLOH, U. G. Factors influencing in vitro infectivity and growth of Rickettsia peacockii (Rickettsiales: Rickettsiaceae), an endosymbiont of the Rocky Mountain 75 wood tick, Dermacentor andersoni (Acari, Ixodidae). Journal of invertebrate pathology, v. 30, n. 3, p. 177-186, 2005. KURTTI, T.J., MUNDERLOH, U.G., STILLER, D. The interaction of Babesia caballi kinetes with tick cells. Journal of Invertebrate Pathology v. 42, n. 3, p. 334?343, 1983. MATTILA, J. T.; MUNDERLOH, U. G.; KURTTI, T. J. Phagocytosis of the Lyme disease spirochete, Borrelia burgdorferi, by cells from the ticks, Ixodes scapularis and Dermacentor andersoni, infected with an endosymbiont, Rickettsia peacockii. Journal of insect science (Online). v. 7, n. 58, p. 1-12, 2007. MATTOS, K. A.; LARA, F. A.; OLIVEIRA, V. G.; RODRIGUES, L. S.; D'AVILA, H.; MELO, R. C.; MANSO, P. P; SARNO, E. N.; BOZZA, P. T.; PESSOLANI, M. C. Modulation of lipid droplets by Mycobacterium leprae in Schwann cells: a putative mechanism for host lipid acquisition and bacterial survival in phagosomes. Cellular Microbiology. v. 13, n. 2, p. 259?273, 2011. MARTIN, H. M.; VIDLER, B. O. In vitro growth of tick tissues Rhipicephalus appendiculatus (Neumann, 1901). Experimental Parasitology, v. 12, n. 1, p. 192-203, 1962. MELO, R. C. N.; DVORAK, A. M. Lipid body-phagosome interaction in macrophages during infectious diseases: host defense or pathogen survival strategy?. PLOS Pathogens, v. 8, n. 7, 2012. MELO, R. C. N.; D?AVILA, H.; WAN, H. C.; BOZZA, P. T.; DVORAK, A. M.; WELLER, P. F. Lipid bodies in inflammatory cells: structure, function, and current imaging techniques. Journal of Histochemistry & Cytochemistry, v. 59, n. 5, p. 540- 556, 2011a. LALLINGER, G.; ZWEYGARTH, E.; BELL-SAKYI, L.; PASSOS, L. M. Cold storage and cryopreservation of tick cell lines. Parasites & Vectors, v. 3, n. 37, p. 1-6, 2010. LEIBO, S. P. ; MAZUR, P. The role of cooling rates in low-temperature preservation. Cryobiology, v. 8, n. 5, p. 447-452, 1971. MANGOLD, A. J.; BARGUES, M. D.; MAS-COMA, S. Mitochondrial 16S rDNA sequences and phylogenetic relationships of species of Rhipicephalus and other tick genera among Metastriata (Acari: Ixodidae). Parasitology Research, v. 84, n. 6, p. 478- 484, 1998. MEDVEDEVA, G. I.; BESKINA, S. R.; GROKHOVSKAYA, I. M. Culture of ixodid tick embryonic cells. Medical Parasitology, v. 41, n. 1, p. 39-40, 1972. MOSQUEDA, J. et al. Primary Midgut, Salivary Gland, and Ovary Cultures from Boophilus microplus. Annals of the New York Academy of Sciences, v. 1149, n. 1, p. 49, 2008. 76 MUNDERLOH, U. G.; BLOUIN, E. F.; KOCAN, K. M.; GE, N. L.; EDWARDS, W. L.; KURTTI, T. J. Establishment of the tick (Acari: Ixodidae) borne cattle pathogen Anaplasma marginale (Rickettsiales: Anaplasmataceae) in tick cell culture. Journal of Medical Entomology, v. 33, n. 4, p. 656-664, 1996. MUNDERLOH, U. G.; JAURON, S. D.; FINGERLE, V.; LEITRITZ, L.; HAYES, S. F.; HAUTMAN, J. M.; NELSON, C. M.; HUBERTY, B. W.; KURTTI, T. J.; AHLSTRAND, G. G.; GREIG, B.; MELLEMCAMP, M. A.; GOODMAN, J. L. Invasion an intracellular development of the human granulocytic ehrlichiosis agent in tick cell culture. Journal of Clinical Microbiology, v. 37, n. 8, p. 2518-2524, 1999. MUNDERLOH, U. G.; KURTTI, T. J. Malarial parasites complete sporogony in axenic mosquitoes. Experientia, v. 41, n. 9, p. 1205-1207, 1985. MUNDERLOH, U. G.; KURTTI, T. J. Formulation of medium for tick cell culture. Experimental & Applied Acarology, v. 7, n. 3, p. 219-229, 1989. MUNDERLOH, U. G.; KURTTI, T. J. Cellular and molecular interrelationships between tick-borne pathogens. Annual Reviews Entomology, v. 40, n. 1, p. 221-243, 1995. MUNDERLOH, U. G.; LIU, Y.; WANG, M.; CHEN, C.; KURTTI, T. J. Establishment, Maintenance and Description of Cell Lines from the Tick Ixodes scapularis. The Journal of Parasitology, v. 80, n. 4, p. 533-543, 1994. MURGIA, R.; CINCO, M. Induction of cystic forms by different stress conditions in Borrelia burgdorferi. APMIS v. 112, n. 1, p. 57?62, 2004. MURRELL, A.; BARKER, S. C. Synonymy of Boophilus Curtice, 1891 with Rhipicephalus Koch, 1844 (Acari: Ixodidae). Systematic Parasitology, v. 56, n. 3, p. 169-172, 2003. NUNES, E. T.; BECHARA, G. H.; SAITO, K. C.; DENARDI, S. E.; OLIVEIRA, P. R.; MATHIAS, M. I. C. Morphological, histological, and ultrastructural characterization of degenerating salivary glands in females of the cattle-tick Rhipicephalus (Boophilus) microplus (CANESTRINI, 1887)(Acari: Ixodidae). Micron, v. 36, n. 5, p. 437-447, 2005. NU?ES, J. L.; MU?OZ COBENAS, M. E.; MOLTEDO, H. L. Boophilus microplus, la garrapata comum del ganado vacuno. Buenos Aires: Hemisf?rio Sur, 1982. 19 p. PASSOS, L. M. F. In vitro cultivation of Anaplasma marginale and A. phagocytophilum in tick cell lines: a review. Revista Brasileira De Parasitologia Veterinaria, v. 21, n. 2, p. 81-86, 2012. PUDNEY, M.; VARMA, M. G. R.; LEAKE, C. J. Culture of embryonic cells from the tick Boophilus microplus (Ixodidae). Journal of Medical Entomology, v. 10, n. 5, p. 493-496, 1973. 77 ?EH??EK, J; BRZOSTOWSKI, H. W. A tick tissue culture medium based on analyses of tick haemolymph. Journal of Insect Physiology, v. 15, n. 8, p. 1431-1436, 1969. REZENDE, J. Cultura prim?ria in vitro de c?lulas embrion?rias de Rhipicephalus (Boophilus) microplus e Amblyomma cajennense como substrato para cultivo de Borrelia burgdorferi. 2008. 22f. Disserta??o (Mestrado em Ci?ncias), Universidade Federal Rural do Rio de Janeiro, Serop?dica, Rio de Janeiro. RIBEIRO, M. F.; BASTOS, C. V.; VASCONCELOS, M. M. C.; PASSOS, L. M. F. < Babesia bigemina: In vitro multiplication of sporokinetes in Ixodes scapularis (IDE8) cells. Experimental parasitology. v. 122, n. 3, p. 192-195, 2009. RITTIG, M. G.; KUHN, K. H.; DECHANT, C. A.; GAUCKLER, A.; MODOLELL, M.; RICCIARDI-CASTAGNOLI, P.; KRAUSE, A.; BURMESTER, G. R. Phagocytes from both vertebrate and invertebrate species use "coiling" phagocytosis. Developmental and Comparative Immunology, v. 20, n. 6, p. 393-406, 1996. RUDENKO, N.; GOLOVCHENKO, M.; NEMEC, J.; VOLKAERT, J.; MALLATOVA, N.; GRUBHOFFER, L. Improved method of detection and molecular typing of Borrelia burgdorferi sensu lato in clinical samples by polymerase chain reaction without DNA purification. Folia Microbiology. v. 50, n. 1, p. 31?39, 2005. SILVA, P. P. An?lise dos corp?sculos lip?dicos em macr?fagos humanos infectados pela cepa Y do Trypanosoma cruzi e submetidos ao tratamento com a droga benzonidazol, 2012, 91f., Disserta??o (Mestrado em Biologia Celular), Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais. STOKER, M. G. P. Role of diffusion boundary layer in contact inhibition of growth. Nature, v. 246, n. 5430, p. 200-203, 1973. TEIXEIRA, R. C. Cultivo de Borrelia burgdorferi (Spirochaetales: Spirochaetaceae) em c?lulas embrion?rias de Rhipicephalus sanguineus (Acari: Ixodidae). 2010. 28f. Disserta??o (Mestrado em Ci?ncias), Universidade Federal Rural do Rio de Janeiro, Serop?dica, Rio de Janeiro. TEIXEIRA, R. C. Cultivo de Borrelia burgdorferi e Borrelia anserina (Spirochaetales: Spirochaetaceae) em diferentes linhagens celulares de carrapatos (Acari: Ixodidae). 2014. 71f. Tese (Doutorado em Ci?ncias), Universidade Federal Rural do Rio de Janeiro, Serop?dica, Rio de Janeiro. VARMA, M. G. R.; PUDNEY, M.; LEAKY, C. J. The establishment of three cell lines from the tick Rhipicephalus appendiculatus (Acari: Ixodidae) and their infection with some arbovirusis. Journal of Medical Entomology, v. 11, n. 6, p. 698-706, 1975. WHARTON, R. H. Ticks with special emphasis on Boophilus microplus. In: PAL, R.; WHARTON, R. H. Control of arthropods of medical and veterinary importance. London Plenum Press, 1974, p. 35-52. 78 WEYER, F. Explantationsversuche bei L?usen in Verbindung mit der Kultur von Rickettsien. Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene, v. 159, n. 1-2, p. 13-22, 1952. WU, J.; WEENING, E. H.; FASKE, J. B.; H??K, M.; SKARE, J. T. Invasion of eukaryotic cells by Borrelia burgdorferi requires ?1 integrins and Src kinase activity. Infection and Immunity, v. 79, n. 3, p. 338?1348, 2011. YUNKER, C. E. Preparation and maintenance of arthropod cell cultures: Acari, with emphasis on ticks. In: YUNKER, C. E. Arboviruses in arthropod cells in vitro. Boca Raton: CRC Press, 1987. p. 35-51. YUNKER, C. E.; CORY, J. Effectiveness of refrigerated nymphs in tick tissue culture experiments. Journal of Parasitology, v. 51, n. 4, p. 686, 1965.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal Rural do Rio de Janeiro
dc.publisher.program.fl_str_mv Programa de P?s-Gradua??o em Ci?ncias Veterin?rias
dc.publisher.initials.fl_str_mv UFRRJ
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Instituto de Veterin?ria
publisher.none.fl_str_mv Universidade Federal Rural do Rio de Janeiro
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ
instname:Universidade Federal Rural do Rio de Janeiro (UFRRJ)
instacron:UFRRJ
instname_str Universidade Federal Rural do Rio de Janeiro (UFRRJ)
instacron_str UFRRJ
institution UFRRJ
reponame_str Biblioteca Digital de Teses e Dissertações da UFRRJ
collection Biblioteca Digital de Teses e Dissertações da UFRRJ
bitstream.url.fl_str_mv http://localhost:8080/tede/bitstream/jspui/2753/18/2015+-+Bruna+de+Azevedo+Ba%C3%AAta.pdf.jpg
http://localhost:8080/tede/bitstream/jspui/2753/17/2015+-+Bruna+de+Azevedo+Ba%C3%AAta.pdf.txt
http://localhost:8080/tede/bitstream/jspui/2753/2/2015+-+Bruna+de+Azevedo+Ba%C3%AAta.pdf
http://localhost:8080/tede/bitstream/jspui/2753/1/license.txt
bitstream.checksum.fl_str_mv cc73c4c239a4c332d642ba1e7c7a9fb2
643c88711930e9fb9b6cf1a3fa780839
4030be45fc06aa6f754bc07f162f640a
7b5ba3d2445355f386edab96125d42b7
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da UFRRJ - Universidade Federal Rural do Rio de Janeiro (UFRRJ)
repository.mail.fl_str_mv bibliot@ufrrj.br||bibliot@ufrrj.br
_version_ 1797220062095998976

Registros relacionados