Estudo fitoqu?mico e farmacol?gico das esp?cies Stachytarpheta cayennensis (Rich.) Vahl (Verbenaceae) e Pleurothyrium bahiense (Meissnes) Barroso (Lauraceae)

Detalhes bibliográficos
Autor(a) principal: Souza, Pierre Andr? de
Data de Publicação: 2004
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://tede.ufrrj.br/jspui/handle/jspui/4220
Resumo: The present dissertation reports the phytochemical and pharmacological/biological study of the roots from Stachytarpheta cayennensis (Rich.) Vahl and the leaves from Pleurothyrium bahiense (Meiss) Barroso. Glycosylated phenylpropanoids and iridoids were isolated from the ethyl acetate extract from the roots of S. cayennensis (Rich.) Vahl by step gradient countercurrent chromatography (CCC). The chosen gradient utilized a stepwise increase of the butanol ratio in a normal phase separation, utilizing a biphasic EtOAc:BuOH:H2O solvent system, 1:X:1, in four steps: A ? X = 0.05, B ? X = 0.2, C ? X = 0.5 and D ? X = 1.0. The sequential increase of BuOH in the organic phase of the CCC solvent system, and wash-off of stationary phase allowed the isolation of 6 pure compounds: martinoside, isoverbascoside, verbascoside, ipolamiide, and two other iridoid glycosides, which structure is being investigated. These compounds covered a far wider span of polarities than could have been achieved by more conventional isocratic CCC. The metanolic extratct of the leaves from Pleurothyrium bahiense had led to the isolation of one steroid: glycosylated ?-sitosterol (PB.1), and one glycosylated flavonoid: Apigenin ?7-O-neohesperidoside. These compounds were identified by their 1H and 13C and infra-red. The 2D heteronuclear NMR techniques for observing coupling through one bond (1H x 1H ? HOMOCOSY, 1H x 13C HETCOSY), 1H x 13C HMQC 1JCH and two or three bonds (1H x 13C HMBC)- COLOC. 1
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spelling Morais, Anselmo AlpandeLeit?o, Gilda Guimar?esMorais, Anselmo AlpandeLeit?o, Gilda Guimar?esMaria Auxiliadora Coelho, KaplanC?rtes, Wellington da SilvaLima, Helena Regina Pinto dehttp://lattes.cnpq.br/3428061204977867Souza, Pierre Andr? de2020-11-30T14:47:10Z2004-11-24Souza, Pierre Andr? de. Estudo fitoqu?mica e farmacol?gico das esp?cies Stachytarpheta cayennensis (Rich.) Vahl (Verbenaceae) e Pleurothyrium bahiense (Meissnes) Barroso (Lauraceae). 2004. 139 f. Disserta??o (Programa de P?s-Gradua??o em Qu?mica) - Universidade Federal Rural do Rio de Janeiro, Serop?dica-RJ .https://tede.ufrrj.br/jspui/handle/jspui/4220The present dissertation reports the phytochemical and pharmacological/biological study of the roots from Stachytarpheta cayennensis (Rich.) Vahl and the leaves from Pleurothyrium bahiense (Meiss) Barroso. Glycosylated phenylpropanoids and iridoids were isolated from the ethyl acetate extract from the roots of S. cayennensis (Rich.) Vahl by step gradient countercurrent chromatography (CCC). The chosen gradient utilized a stepwise increase of the butanol ratio in a normal phase separation, utilizing a biphasic EtOAc:BuOH:H2O solvent system, 1:X:1, in four steps: A ? X = 0.05, B ? X = 0.2, C ? X = 0.5 and D ? X = 1.0. The sequential increase of BuOH in the organic phase of the CCC solvent system, and wash-off of stationary phase allowed the isolation of 6 pure compounds: martinoside, isoverbascoside, verbascoside, ipolamiide, and two other iridoid glycosides, which structure is being investigated. These compounds covered a far wider span of polarities than could have been achieved by more conventional isocratic CCC. The metanolic extratct of the leaves from Pleurothyrium bahiense had led to the isolation of one steroid: glycosylated ?-sitosterol (PB.1), and one glycosylated flavonoid: Apigenin ?7-O-neohesperidoside. These compounds were identified by their 1H and 13C and infra-red. The 2D heteronuclear NMR techniques for observing coupling through one bond (1H x 1H ? HOMOCOSY, 1H x 13C HETCOSY), 1H x 13C HMQC 1JCH and two or three bonds (1H x 13C HMBC)- COLOC. 1Essa disserta??o envolveu o estudo fitoqu?mico e farmacol?gico (atividade antiinflamat?ria, antinociceptiva, antimicrobiana e antioxidante), e an?lise de solos das ?reas de coleta de ocorr?ncia end?mica das esp?cies Stachytarpheta cayennensis (Rich.) Vahl (Ra?zes) e Pleurothyrium bahiense (Meiss) Barroso (Folhas). A esp?cie S. cayennensis foi coletada em Florian?polis na regi?o do canto dos Ara??s, e a esp?cie P. bahiense foi coletada no Rio de Janeiro na regi?o do Maci?o da Pedra Branca. Os arilpropan?ides glicosilados e irid?ides glicosilados das ra?zes de S. cayennensis (Rich.) Vahl foram isolados da fra??o acetato de etila, por meio de cromatografia contracorrente (CCC), com gradiente de polaridade. A escolha de um gradiente n?o-linear, utilizando-se o sistema de solventes bif?sico EtOAc: BuOH: H2O, em quatro etapas, A ? X = 0.05, B ? X = 0.2, C ? X = 0.5 e D ? X = 1.0 teve o BuOH (X) aumentado gradativamente por sua maior afinidade com a fase org?nica, por ser este sistema comumente usado para subst?ncias polares. Obteve-se desse fracionamento o isolamento de 6 subst?ncias puras: verbascos?deo (I), isoverbascos?deo (II), martinos?deo (III), ipolamiida (IV) e outros dois irid?ides em fase de identifica??o. A fra??o metan?lica das folhas da esp?cie P. bahiense (Meiss) Barroso, por cromatografia em coluna de gel de s?lica, conduziu ao isolamento de um ester?ide: ?- sitosterol glicosilado (PB.1), e um flavon?ide glicosilado (PB.2): Apigenina-7-O-neohesperidos?deo. Essas subst?ncias foram identificadas e suas estruturas elucidadas por meio de t?cnicas espectrosc?picas de RMN 1H, RMN 13C e IV. Bem como t?cnicas bidimensionais (2D) de RMN observando-se a constante de acoplamento de uma liga??o (1H x 1H ? HOMOCOSY, 1H x 13C HETCOSY), 1H x 13C HMBQC 1JCH e duas ou tr?s liga??es (1H x 13C HMBC).Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2020-11-30T14:47:10Z No. of bitstreams: 1 2004 - Pierre Andr? de Souza.pdf: 3451221 bytes, checksum: cf25110fcae390b83ac6c85a03f22b41 (MD5)Made available in DSpace on 2020-11-30T14:47:10Z (GMT). No. of bitstreams: 1 2004 - Pierre Andr? de Souza.pdf: 3451221 bytes, checksum: cf25110fcae390b83ac6c85a03f22b41 (MD5) Previous issue date: 2004-11-24Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPESapplication/pdfhttps://tede.ufrrj.br/retrieve/63294/2004%20-%20Pierre%20Andr%c3%a9%20de%20Souza.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Qu?micaUFRRJBrasilInstituto de Ci?ncias ExatasABBOT P.T., KLEIMAN R. 1991. Solvent selection guide for counter-current chromatography. 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Estudos da Toler?ncia ao Alum?nio em Arroz de Sequeiro e Seus Efeitos Sobre a Interface Solo-Planta. Tese de Doutorado.UFRRJ. Rio de Janeiroan?lise de solosestudo fitoqu?micoatividade antiinflamat?ria, antinociceptiva, antimicrobiana e antioxidanteQu?micaEstudo fitoqu?mico e farmacol?gico das esp?cies Stachytarpheta cayennensis (Rich.) 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dc.title.por.fl_str_mv Estudo fitoqu?mico e farmacol?gico das esp?cies Stachytarpheta cayennensis (Rich.) Vahl (Verbenaceae) e Pleurothyrium bahiense (Meissnes) Barroso (Lauraceae)
title Estudo fitoqu?mico e farmacol?gico das esp?cies Stachytarpheta cayennensis (Rich.) Vahl (Verbenaceae) e Pleurothyrium bahiense (Meissnes) Barroso (Lauraceae)
spellingShingle Estudo fitoqu?mico e farmacol?gico das esp?cies Stachytarpheta cayennensis (Rich.) Vahl (Verbenaceae) e Pleurothyrium bahiense (Meissnes) Barroso (Lauraceae)
Souza, Pierre Andr? de
an?lise de solos
estudo fitoqu?mico
atividade antiinflamat?ria, antinociceptiva, antimicrobiana e antioxidante
Qu?mica
title_short Estudo fitoqu?mico e farmacol?gico das esp?cies Stachytarpheta cayennensis (Rich.) Vahl (Verbenaceae) e Pleurothyrium bahiense (Meissnes) Barroso (Lauraceae)
title_full Estudo fitoqu?mico e farmacol?gico das esp?cies Stachytarpheta cayennensis (Rich.) Vahl (Verbenaceae) e Pleurothyrium bahiense (Meissnes) Barroso (Lauraceae)
title_fullStr Estudo fitoqu?mico e farmacol?gico das esp?cies Stachytarpheta cayennensis (Rich.) Vahl (Verbenaceae) e Pleurothyrium bahiense (Meissnes) Barroso (Lauraceae)
title_full_unstemmed Estudo fitoqu?mico e farmacol?gico das esp?cies Stachytarpheta cayennensis (Rich.) Vahl (Verbenaceae) e Pleurothyrium bahiense (Meissnes) Barroso (Lauraceae)
title_sort Estudo fitoqu?mico e farmacol?gico das esp?cies Stachytarpheta cayennensis (Rich.) Vahl (Verbenaceae) e Pleurothyrium bahiense (Meissnes) Barroso (Lauraceae)
author Souza, Pierre Andr? de
author_facet Souza, Pierre Andr? de
author_role author
dc.contributor.advisor1.fl_str_mv Morais, Anselmo Alpande
dc.contributor.advisor-co1.fl_str_mv Leit?o, Gilda Guimar?es
dc.contributor.referee1.fl_str_mv Morais, Anselmo Alpande
dc.contributor.referee2.fl_str_mv Leit?o, Gilda Guimar?es
dc.contributor.referee3.fl_str_mv Maria Auxiliadora Coelho, Kaplan
dc.contributor.referee4.fl_str_mv C?rtes, Wellington da Silva
dc.contributor.referee5.fl_str_mv Lima, Helena Regina Pinto de
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/3428061204977867
dc.contributor.author.fl_str_mv Souza, Pierre Andr? de
contributor_str_mv Morais, Anselmo Alpande
Leit?o, Gilda Guimar?es
Morais, Anselmo Alpande
Leit?o, Gilda Guimar?es
Maria Auxiliadora Coelho, Kaplan
C?rtes, Wellington da Silva
Lima, Helena Regina Pinto de
dc.subject.por.fl_str_mv an?lise de solos
estudo fitoqu?mico
atividade antiinflamat?ria, antinociceptiva, antimicrobiana e antioxidante
topic an?lise de solos
estudo fitoqu?mico
atividade antiinflamat?ria, antinociceptiva, antimicrobiana e antioxidante
Qu?mica
dc.subject.cnpq.fl_str_mv Qu?mica
description The present dissertation reports the phytochemical and pharmacological/biological study of the roots from Stachytarpheta cayennensis (Rich.) Vahl and the leaves from Pleurothyrium bahiense (Meiss) Barroso. Glycosylated phenylpropanoids and iridoids were isolated from the ethyl acetate extract from the roots of S. cayennensis (Rich.) Vahl by step gradient countercurrent chromatography (CCC). The chosen gradient utilized a stepwise increase of the butanol ratio in a normal phase separation, utilizing a biphasic EtOAc:BuOH:H2O solvent system, 1:X:1, in four steps: A ? X = 0.05, B ? X = 0.2, C ? X = 0.5 and D ? X = 1.0. The sequential increase of BuOH in the organic phase of the CCC solvent system, and wash-off of stationary phase allowed the isolation of 6 pure compounds: martinoside, isoverbascoside, verbascoside, ipolamiide, and two other iridoid glycosides, which structure is being investigated. These compounds covered a far wider span of polarities than could have been achieved by more conventional isocratic CCC. The metanolic extratct of the leaves from Pleurothyrium bahiense had led to the isolation of one steroid: glycosylated ?-sitosterol (PB.1), and one glycosylated flavonoid: Apigenin ?7-O-neohesperidoside. These compounds were identified by their 1H and 13C and infra-red. The 2D heteronuclear NMR techniques for observing coupling through one bond (1H x 1H ? HOMOCOSY, 1H x 13C HETCOSY), 1H x 13C HMQC 1JCH and two or three bonds (1H x 13C HMBC)- COLOC. 1
publishDate 2004
dc.date.issued.fl_str_mv 2004-11-24
dc.date.accessioned.fl_str_mv 2020-11-30T14:47:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv Souza, Pierre Andr? de. Estudo fitoqu?mica e farmacol?gico das esp?cies Stachytarpheta cayennensis (Rich.) Vahl (Verbenaceae) e Pleurothyrium bahiense (Meissnes) Barroso (Lauraceae). 2004. 139 f. Disserta??o (Programa de P?s-Gradua??o em Qu?mica) - Universidade Federal Rural do Rio de Janeiro, Serop?dica-RJ .
dc.identifier.uri.fl_str_mv https://tede.ufrrj.br/jspui/handle/jspui/4220
identifier_str_mv Souza, Pierre Andr? de. Estudo fitoqu?mica e farmacol?gico das esp?cies Stachytarpheta cayennensis (Rich.) Vahl (Verbenaceae) e Pleurothyrium bahiense (Meissnes) Barroso (Lauraceae). 2004. 139 f. Disserta??o (Programa de P?s-Gradua??o em Qu?mica) - Universidade Federal Rural do Rio de Janeiro, Serop?dica-RJ .
url https://tede.ufrrj.br/jspui/handle/jspui/4220
dc.language.iso.fl_str_mv por
language por
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