Diversidade de bact?rias do g?nero Bradyrhizobium como endof?ticas e na rizosfera de duas cultivares de cana-de-a??car
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRRJ |
Texto Completo: | https://tede.ufrrj.br/jspui/handle/jspui/2604 |
Resumo: | The genus Bradyrhizobium is commonly found as endosymbiont in legumes (Fabaceae family), such as soybean (Glycine max), forming nodules on the roots and providing nitrogen from the atmosphere to these plants and to the soil. Recent research has shown the presence of bacteria from this genus inside roots of sugarcane (Saccharum spp.), a non-legume belonging to the Poaceae family. This work aims to know the population density of these bacteria associated with the roots of two sugarcane cultivars and to understand how the plant genotype (cultivar) affects the population composition of these bacteria found in the rhizosphere and the endosphere. Rhizosphere and root samples of two economically relevant sugarcane cultivars (RB867515 and IACSP95-5000) were collected in the experimental field of Embrapa Agrobiology, serially diluted and inoculated on gnotobiotic seedlings of cowpea used as trap plants. The most probable number technique was used to quantify the density of the rhizobia present in the samples and the isolates obtained from the nodules were cultivated to form pure cultures. BOX-PCR technique and sequence analysis of the ribosomal internally transcribed spacer region (ITS), recA housekeeping gene, and the symbiotic nodC gene were applied to better understand the genetic diversity of these rhizobia. Acetylene reduction test was applied to verify the presence of nitrogenase activity under free-living conditions. The presence of at least 15625 rhizobia g root -1 in rhizosphere samples and between 125 and 625 rhizobia g root-1 in the endosphere samples was observed. This shows that the density of rhizobia in the rhizosphere of sugarcane is significantly higher than in the interior of the roots for the two cane cultivars. A total of 34 isolates were obtained from the rhizosphere samples of each cultivar and 15 and 14 respectively from the roots of cultivars IACSP95-5000 and RB867515. Most of the isolates presented morphoculture characteristics of the genus Bradyrhizobium. Phylogenetic analyzes positioned most of the isolates in the superclade Bradyrhizobium elkanii. No correlation was observed between the plant cultivar and the abundance of Bradyrhizobium spp. in the roots and rhizosphere of sugarcane plants. |
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Rouws, Luc Felicianus Marie014.563.806-56Jesus, Ederson da Concei??o084.646.497-70Rouws, Luc Felicianus MarieCoelho, Irene da SilvaCoelho, Marcia Reed Rodrigues082.666.937-90http://lattes.cnpq.br/3333061625652193Menezes J?nior, Ivan de Alencar2019-03-20T18:21:10Z2017-04-27MENEZES J?NIOR, Ivan de Alencar. Diversidade de bact?rias do g?nero Bradyrhizobium como endof?ticas e na rizosfera de duas cultivares de cana-de-a??car. 2017. 77 f. Disserta??o (Mestrado em Fitossanidade e Biotecnologia Aplicada) - Instituto de Ci?ncias Biol?gicas e da Sa?de, Departamento de Entomologia e Fitopatologia, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2017.https://tede.ufrrj.br/jspui/handle/jspui/2604The genus Bradyrhizobium is commonly found as endosymbiont in legumes (Fabaceae family), such as soybean (Glycine max), forming nodules on the roots and providing nitrogen from the atmosphere to these plants and to the soil. Recent research has shown the presence of bacteria from this genus inside roots of sugarcane (Saccharum spp.), a non-legume belonging to the Poaceae family. This work aims to know the population density of these bacteria associated with the roots of two sugarcane cultivars and to understand how the plant genotype (cultivar) affects the population composition of these bacteria found in the rhizosphere and the endosphere. Rhizosphere and root samples of two economically relevant sugarcane cultivars (RB867515 and IACSP95-5000) were collected in the experimental field of Embrapa Agrobiology, serially diluted and inoculated on gnotobiotic seedlings of cowpea used as trap plants. The most probable number technique was used to quantify the density of the rhizobia present in the samples and the isolates obtained from the nodules were cultivated to form pure cultures. BOX-PCR technique and sequence analysis of the ribosomal internally transcribed spacer region (ITS), recA housekeeping gene, and the symbiotic nodC gene were applied to better understand the genetic diversity of these rhizobia. Acetylene reduction test was applied to verify the presence of nitrogenase activity under free-living conditions. The presence of at least 15625 rhizobia g root -1 in rhizosphere samples and between 125 and 625 rhizobia g root-1 in the endosphere samples was observed. This shows that the density of rhizobia in the rhizosphere of sugarcane is significantly higher than in the interior of the roots for the two cane cultivars. A total of 34 isolates were obtained from the rhizosphere samples of each cultivar and 15 and 14 respectively from the roots of cultivars IACSP95-5000 and RB867515. Most of the isolates presented morphoculture characteristics of the genus Bradyrhizobium. Phylogenetic analyzes positioned most of the isolates in the superclade Bradyrhizobium elkanii. No correlation was observed between the plant cultivar and the abundance of Bradyrhizobium spp. in the roots and rhizosphere of sugarcane plants.O g?nero Bradyrhizobium ? comumente encontrado como endossimbionte em leguminosas (fam?lia Fabaceae) como a soja (Glycine max), formando n?dulos nas ra?zes dessas plantas e disponibilizando nitrog?nio da atmosfera para essas plantas e para o solo. Pesquisas recentes mostraram a presen?a de bact?rias desse g?nero no interior de ra?zes de cana-de-a??car (Saccharum spp.), uma n?o leguminosa pertencente ? fam?lia Poaceae. Este trabalho tem como objetivo conhecer a densidade populacional dessas bact?rias associadas ?s ra?zes de duas cultivares de cana-de-a??car e compreender como o gen?tipo da planta (cultivar) afeta a composi??o populacional dessas bact?rias encontradas na rizosfera e na endosfera. Amostras de rizosfera e de ra?zes de duas cultivares econ?micamente relevantes de cana-de-a??car (RB867515 e IACSP95-5000) foram coletadas no campo experimental da Embrapa Agrobiologia, seriadamente dilu?das e inoculadas em pl?ntulas gnotobi?ticas de feij?o-caupi usadas como plantas iscas. A t?cnica de n?mero mais prov?vel foi utilizada para quantificar a densidade dos riz?bios presentes nas amostras e os isolados obtidos dos n?dulos foram cultivados at? formarem culturas puras. A t?cnica de BOX-PCR e an?lise de sequ?ncia dos genes recA, regi?o ITS e nodC foram aplicadas para compreender melhor a diversidade gen?tica desses riz?bios. Ensaio de redu??o de acetileno foi aplicado para verificar a presen?a de atividade da nitrogenase. Observou-se a presen?a de no m?nimo 15625 riz?bios g -1 de raiz em amostras de rizosfera e entre 125 e 625 riz?bios g -1 de raiz nas amostras de endosfera. Isso mostra que a densidade de riz?bios na rizosfera de cana-de-a??car ? significantemente maior do que no interior das ra?zes para as duas cultivares de cana. Foram obtidos 34 isolados das amostras de rizosfera de cada cultivar e 15 e 14 respectivamente do interior das ra?zes das cultivares IACSP95-5000 e RB867515. A maior parte dos isolados apresentaram caracter?sticas morfoculturais do g?nero Bradyrhizobium. An?lises filogen?ticas posicionaram a maioria dos isolados no superclado de Bradyrhizobium elkanii. N?o foi observada correla??o entre a cultivar vegetal e a abund?ncia de Bradyrhizobium. spp. nas ra?zes e rizosfera das plantas de cana-de-a??car.Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2019-03-20T18:21:10Z No. of bitstreams: 1 2017 - Ivan de Alencar Menezes J?nior.pdf: 2048768 bytes, checksum: 10fd425fb0df338a0e4159e5c6a4d1ba (MD5)Made available in DSpace on 2019-03-20T18:21:10Z (GMT). No. of bitstreams: 1 2017 - Ivan de Alencar Menezes J?nior.pdf: 2048768 bytes, checksum: 10fd425fb0df338a0e4159e5c6a4d1ba (MD5) Previous issue date: 2017-04-27CAPESapplication/pdfhttps://tede.ufrrj.br/retrieve/9369/2017%20-%20Ivan%20de%20Alencar%20Menezes%20J%c3%banior.pdf.jpghttps://tede.ufrrj.br/retrieve/15680/2017%20-%20Ivan%20de%20Alencar%20Menezes%20J%c3%banior.pdf.jpghttps://tede.ufrrj.br/retrieve/21982/2017%20-%20Ivan%20de%20Alencar%20Menezes%20J%c3%banior.pdf.jpghttps://tede.ufrrj.br/retrieve/28364/2017%20-%20Ivan%20de%20Alencar%20Menezes%20J%c3%banior.pdf.jpghttps://tede.ufrrj.br/retrieve/34778/2017%20-%20Ivan%20de%20Alencar%20Menezes%20J%c3%banior.pdf.jpghttps://tede.ufrrj.br/retrieve/41174/2017%20-%20Ivan%20de%20Alencar%20Menezes%20J%c3%banior.pdf.jpghttps://tede.ufrrj.br/retrieve/47576/2017%20-%20Ivan%20de%20Alencar%20Menezes%20J%c3%banior.pdf.jpghttps://tede.ufrrj.br/retrieve/54014/2017%20-%20Ivan%20de%20Alencar%20Menezes%20J%c3%banior.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Fitossanidade e Biotecnologia AplicadaUFRRJBrasilInstituto de Ci?ncias Biol?gicas e da Sa?deFixa??o Biol?gica de Nitrog?niobact?rias diazotr?ficasgene nifHBiological nitrogen fixationdiazotrophic bacterianifH geneAgronomiaDiversidade de bact?rias do g?nero Bradyrhizobium como endof?ticas e na rizosfera de duas cultivares de cana-de-a??carinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRRJinstname:Universidade Federal Rural do Rio de Janeiro (UFRRJ)instacron:UFRRJTEXT2017 - Ivan de Alencar Menezes J?nior.pdf.txt2017 - Ivan de Alencar Menezes J?nior.pdf.txttext/plain212627http://localhost:8080/tede/bitstream/jspui/2604/17/2017+-+Ivan+de+Alencar+Menezes+J%C3%BAnior.pdf.txt906db364b7eaab0e79ecc419cddaefffMD517ORIGINAL2017 - Ivan de Alencar Menezes J?nior.pdf2017 - Ivan de Alencar Menezes J?nior.pdfapplication/pdf1963014http://localhost:8080/tede/bitstream/jspui/2604/4/2017+-+Ivan+de+Alencar+Menezes+J%C3%BAnior.pdf4398fd87111f6eb9f28676bd3e6350d3MD54THUMBNAIL2017 - Ivan de Alencar Menezes J?nior.pdf.jpg2017 - Ivan de Alencar Menezes J?nior.pdf.jpgimage/jpeg1943http://localhost:8080/tede/bitstream/jspui/2604/18/2017+-+Ivan+de+Alencar+Menezes+J%C3%BAnior.pdf.jpgcc73c4c239a4c332d642ba1e7c7a9fb2MD518LICENSElicense.txtlicense.txttext/plain; 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dc.title.por.fl_str_mv |
Diversidade de bact?rias do g?nero Bradyrhizobium como endof?ticas e na rizosfera de duas cultivares de cana-de-a??car |
title |
Diversidade de bact?rias do g?nero Bradyrhizobium como endof?ticas e na rizosfera de duas cultivares de cana-de-a??car |
spellingShingle |
Diversidade de bact?rias do g?nero Bradyrhizobium como endof?ticas e na rizosfera de duas cultivares de cana-de-a??car Menezes J?nior, Ivan de Alencar Fixa??o Biol?gica de Nitrog?nio bact?rias diazotr?ficas gene nifH Biological nitrogen fixation diazotrophic bacteria nifH gene Agronomia |
title_short |
Diversidade de bact?rias do g?nero Bradyrhizobium como endof?ticas e na rizosfera de duas cultivares de cana-de-a??car |
title_full |
Diversidade de bact?rias do g?nero Bradyrhizobium como endof?ticas e na rizosfera de duas cultivares de cana-de-a??car |
title_fullStr |
Diversidade de bact?rias do g?nero Bradyrhizobium como endof?ticas e na rizosfera de duas cultivares de cana-de-a??car |
title_full_unstemmed |
Diversidade de bact?rias do g?nero Bradyrhizobium como endof?ticas e na rizosfera de duas cultivares de cana-de-a??car |
title_sort |
Diversidade de bact?rias do g?nero Bradyrhizobium como endof?ticas e na rizosfera de duas cultivares de cana-de-a??car |
author |
Menezes J?nior, Ivan de Alencar |
author_facet |
Menezes J?nior, Ivan de Alencar |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Rouws, Luc Felicianus Marie |
dc.contributor.advisor1ID.fl_str_mv |
014.563.806-56 |
dc.contributor.advisor-co1.fl_str_mv |
Jesus, Ederson da Concei??o |
dc.contributor.advisor-co1ID.fl_str_mv |
084.646.497-70 |
dc.contributor.referee1.fl_str_mv |
Rouws, Luc Felicianus Marie |
dc.contributor.referee2.fl_str_mv |
Coelho, Irene da Silva |
dc.contributor.referee3.fl_str_mv |
Coelho, Marcia Reed Rodrigues |
dc.contributor.authorID.fl_str_mv |
082.666.937-90 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3333061625652193 |
dc.contributor.author.fl_str_mv |
Menezes J?nior, Ivan de Alencar |
contributor_str_mv |
Rouws, Luc Felicianus Marie Jesus, Ederson da Concei??o Rouws, Luc Felicianus Marie Coelho, Irene da Silva Coelho, Marcia Reed Rodrigues |
dc.subject.por.fl_str_mv |
Fixa??o Biol?gica de Nitrog?nio bact?rias diazotr?ficas gene nifH |
topic |
Fixa??o Biol?gica de Nitrog?nio bact?rias diazotr?ficas gene nifH Biological nitrogen fixation diazotrophic bacteria nifH gene Agronomia |
dc.subject.eng.fl_str_mv |
Biological nitrogen fixation diazotrophic bacteria nifH gene |
dc.subject.cnpq.fl_str_mv |
Agronomia |
description |
The genus Bradyrhizobium is commonly found as endosymbiont in legumes (Fabaceae family), such as soybean (Glycine max), forming nodules on the roots and providing nitrogen from the atmosphere to these plants and to the soil. Recent research has shown the presence of bacteria from this genus inside roots of sugarcane (Saccharum spp.), a non-legume belonging to the Poaceae family. This work aims to know the population density of these bacteria associated with the roots of two sugarcane cultivars and to understand how the plant genotype (cultivar) affects the population composition of these bacteria found in the rhizosphere and the endosphere. Rhizosphere and root samples of two economically relevant sugarcane cultivars (RB867515 and IACSP95-5000) were collected in the experimental field of Embrapa Agrobiology, serially diluted and inoculated on gnotobiotic seedlings of cowpea used as trap plants. The most probable number technique was used to quantify the density of the rhizobia present in the samples and the isolates obtained from the nodules were cultivated to form pure cultures. BOX-PCR technique and sequence analysis of the ribosomal internally transcribed spacer region (ITS), recA housekeeping gene, and the symbiotic nodC gene were applied to better understand the genetic diversity of these rhizobia. Acetylene reduction test was applied to verify the presence of nitrogenase activity under free-living conditions. The presence of at least 15625 rhizobia g root -1 in rhizosphere samples and between 125 and 625 rhizobia g root-1 in the endosphere samples was observed. This shows that the density of rhizobia in the rhizosphere of sugarcane is significantly higher than in the interior of the roots for the two cane cultivars. A total of 34 isolates were obtained from the rhizosphere samples of each cultivar and 15 and 14 respectively from the roots of cultivars IACSP95-5000 and RB867515. Most of the isolates presented morphoculture characteristics of the genus Bradyrhizobium. Phylogenetic analyzes positioned most of the isolates in the superclade Bradyrhizobium elkanii. No correlation was observed between the plant cultivar and the abundance of Bradyrhizobium spp. in the roots and rhizosphere of sugarcane plants. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-04-27 |
dc.date.accessioned.fl_str_mv |
2019-03-20T18:21:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MENEZES J?NIOR, Ivan de Alencar. Diversidade de bact?rias do g?nero Bradyrhizobium como endof?ticas e na rizosfera de duas cultivares de cana-de-a??car. 2017. 77 f. Disserta??o (Mestrado em Fitossanidade e Biotecnologia Aplicada) - Instituto de Ci?ncias Biol?gicas e da Sa?de, Departamento de Entomologia e Fitopatologia, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2017. |
dc.identifier.uri.fl_str_mv |
https://tede.ufrrj.br/jspui/handle/jspui/2604 |
identifier_str_mv |
MENEZES J?NIOR, Ivan de Alencar. Diversidade de bact?rias do g?nero Bradyrhizobium como endof?ticas e na rizosfera de duas cultivares de cana-de-a??car. 2017. 77 f. Disserta??o (Mestrado em Fitossanidade e Biotecnologia Aplicada) - Instituto de Ci?ncias Biol?gicas e da Sa?de, Departamento de Entomologia e Fitopatologia, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2017. |
url |
https://tede.ufrrj.br/jspui/handle/jspui/2604 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural do Rio de Janeiro |
dc.publisher.program.fl_str_mv |
Programa de P?s-Gradua??o em Fitossanidade e Biotecnologia Aplicada |
dc.publisher.initials.fl_str_mv |
UFRRJ |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Instituto de Ci?ncias Biol?gicas e da Sa?de |
publisher.none.fl_str_mv |
Universidade Federal Rural do Rio de Janeiro |
dc.source.none.fl_str_mv |
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UFRRJ |
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Biblioteca Digital de Teses e Dissertações da UFRRJ |
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Biblioteca Digital de Teses e Dissertações da UFRRJ - Universidade Federal Rural do Rio de Janeiro (UFRRJ) |
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