A import?ncia do pombo urbano (Columba livia) na dissemina??o ambiental de Cryptosporidium spp

Detalhes bibliográficos
Autor(a) principal: Costa, Alexandre de Pina
Data de Publicação: 2017
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://tede.ufrrj.br/jspui/handle/jspui/5850
Resumo: The urban pigeon (Columba livia) is a bird frequently observed in urban centers, living in close contact with humans, being a possible disseminator of pathogens in the environment. Among these, special attention should be given to the species of Cryptosporidium, since the pigeon can harbor and eliminate the oocysts through the faeces. To date, three species are considered valid parasitizing birds (Cryptosporidium meleagridis, Cryptosporidium baileyi and Cryptosporidium galli) and 13 genotypes as possible agents of avian cryptosporidiosis. As the first species, besides parasitizing the birds class, has been diagnosed in several mammalian hosts, also zoonotic. Due to the lack of studies on the diagnosis of Cryptosporidium spp. parasitizing pigeons in Brazil and with few reports in the world, the present work had as objectives: to diagnose microscopically the presence of oocysts of Cryptosporidium spp. from pigeons faecal samples captured in three areas in the Rio de Janeiro city; to characterize genotypes and / or genotypes and / or subtypes obtained from those positive samples for Cryptosporidium spp. and perform sequencing and phylogenetic analyzes by comparing the sequences obtained with those sequences deposited in GenBank. A total of 387 pigeons fecal samples were collected in three administrative regions: Campo Grande - denominated A (98 samples), Bangu - denominated B (187 samples) and Madureira - denominated C (102 samples). Initially the Cryptosporidium spp. oocysts were investigated. For this, the faeces were processed through the technique of centrifugation and flotation in saturated sugar solution and evaluated by light microscopy. In the microscopic diagnosis, 81 (20.93%) samples were positive for the presence of Cryptosporidium spp. oocysts, distributed: 28.82% in area A, 22.99% in area B and 9.80% in area C. In these, DNA extraction was performed, followed by amplification and sequencing for the 18S target. Of these 81, 53 samples amplified in Nested-PCR. Subsequently, these samples were purified and sent for sequencing, followed by phylogenetic analyzes. In this study, two species were diagnosed: Cryptosporidium meleagridis in 44 samples (91.67%) and Cryptosporidum baileyi in four samples (8.33%), both of which were more closely related to intestinal species. Sequences of seven samples containing different nucleotides of Cryptosporidum were deposited in Genbank. So far, the report of this pathogen in pigeons had only occurred in four countries in the world: Turkey, Iran, China and Thailand. The data obtained in this study reflect public health concern, and C. meleagridis may present zoonotic potential. This study is the first Brazilian registry to have the host pigeon (Columba livia) infected by Cryptosporidium, contributing to trace the molecular epidemiology of this etiological agent in the country
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spelling Bomfim, Teresa Cristina Bergamo do609.928.087-15https://orcid.org/0000-0002-6741-0202http://lattes.cnpq.br/6299581965425007Bomfim, Teresa Cristina Bergamo do609.928.087-15https://orcid.org/0000-0002-6741-0202http://lattes.cnpq.br/6299581965425007Sudr? , Adriana Pittellahttps://orcid.org/0000-0002-0386-6278http://lattes.cnpq.br/1820344065898657Santos, Helena L?cia Carneirohttps://orcid.org/0000-0002-5240-5662http://lattes.cnpq.br/4847592947227424Toma, Helena Keikohttp://lattes.cnpq.br/8121311753666435Barros, Luciano Antuneshttp://lattes.cnpq.br/8336187135982826032.513.387-57https://orcid.org/0000-0002-4228-5118http://lattes.cnpq.br/7288645391409152Costa, Alexandre de Pina2022-08-05T14:05:00Z2017-03-09COSTA, Alexandre de Pina. A import?ncia do pombo urbano (Columba livia) na dissemina??o ambiental de Cryptosporidium spp. 2017. 96 f. Tese (Doutorado em Ci?ncias Veterin?riasa) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2017https://tede.ufrrj.br/jspui/handle/jspui/5850The urban pigeon (Columba livia) is a bird frequently observed in urban centers, living in close contact with humans, being a possible disseminator of pathogens in the environment. Among these, special attention should be given to the species of Cryptosporidium, since the pigeon can harbor and eliminate the oocysts through the faeces. To date, three species are considered valid parasitizing birds (Cryptosporidium meleagridis, Cryptosporidium baileyi and Cryptosporidium galli) and 13 genotypes as possible agents of avian cryptosporidiosis. As the first species, besides parasitizing the birds class, has been diagnosed in several mammalian hosts, also zoonotic. Due to the lack of studies on the diagnosis of Cryptosporidium spp. parasitizing pigeons in Brazil and with few reports in the world, the present work had as objectives: to diagnose microscopically the presence of oocysts of Cryptosporidium spp. from pigeons faecal samples captured in three areas in the Rio de Janeiro city; to characterize genotypes and / or genotypes and / or subtypes obtained from those positive samples for Cryptosporidium spp. and perform sequencing and phylogenetic analyzes by comparing the sequences obtained with those sequences deposited in GenBank. A total of 387 pigeons fecal samples were collected in three administrative regions: Campo Grande - denominated A (98 samples), Bangu - denominated B (187 samples) and Madureira - denominated C (102 samples). Initially the Cryptosporidium spp. oocysts were investigated. For this, the faeces were processed through the technique of centrifugation and flotation in saturated sugar solution and evaluated by light microscopy. In the microscopic diagnosis, 81 (20.93%) samples were positive for the presence of Cryptosporidium spp. oocysts, distributed: 28.82% in area A, 22.99% in area B and 9.80% in area C. In these, DNA extraction was performed, followed by amplification and sequencing for the 18S target. Of these 81, 53 samples amplified in Nested-PCR. Subsequently, these samples were purified and sent for sequencing, followed by phylogenetic analyzes. In this study, two species were diagnosed: Cryptosporidium meleagridis in 44 samples (91.67%) and Cryptosporidum baileyi in four samples (8.33%), both of which were more closely related to intestinal species. Sequences of seven samples containing different nucleotides of Cryptosporidum were deposited in Genbank. So far, the report of this pathogen in pigeons had only occurred in four countries in the world: Turkey, Iran, China and Thailand. The data obtained in this study reflect public health concern, and C. meleagridis may present zoonotic potential. This study is the first Brazilian registry to have the host pigeon (Columba livia) infected by Cryptosporidium, contributing to trace the molecular epidemiology of this etiological agent in the countryO pombo urbano (Columba livia), ? uma ave frequentemente observada em centros urbanos, vivendo em estreito contato com humanos, sendo um poss?vel disseminador de pat?genos no meio ambiente. Dentre estes, uma aten??o especial deve ser dada ?s esp?cies de Cryptosporidium, j? que o pombo pode albergar e eliminar oocistos atrav?s das fezes. At? o momento, tr?s esp?cies s?o consideradas como v?lidas parasitando aves (Cryptosporidium meleagridis, Cryptosporidium baileyi e Cryptosporidium galli) e 13 gen?tipos como poss?veis agentes da criptosporidiose avi?ria. Sendo que a primeira esp?cie, al?m de parasitar a classe das aves, tem sido diagnosticada em v?rios hospedeiros mam?feros, tamb?m com car?ter zoon?tico. Devido a aus?ncia de estudos sobre o diagn?stico de Cryptosporidium spp. parasitando pombos no Brasil e com escassos relatos no mundo, o presente trabalho teve como objetivos: diagnosticar microscopicamente a presen?a de oocistos de Cryptosporidium spp. provenientes de amostras fecais de pombos capturados em tr?s ?reas no munic?pio do Rio de Janeiro; caracterizar genotipicamente esp?cies e/ou gen?tipos e/ou subtipos obtidos daquelas amostras positivas para Cryptosporidium spp. e realizar o sequenciamento e an?lises filogen?ticas, comparando as sequencias obtidas com aquelas sequ?ncias depositadas no GenBank. Foram coletados um total de 387 amostras fecais de pombos, em tr?s regi?es administrativas: Campo Grande ? denominada de A (98 amostras), Bangu ? denominada de B (187 amostras) e Madureira ? denominada de C (102 amostras). Inicialmente foi realizada a pesquisa de oocistos Cryptosporidium spp., para tal, as fezes foram processadas atrav?s da t?cnica de centrifuga??o e flutua??o em solu??o saturada de a??car e avaliadas por microscopia ?ptica. No diagn?stico microsc?pico, 81 (20,93%) amostras apresentaram positividade para a presen?a de oocistos de Cryptosporidium spp., distribu?dos: 28,82% na ?rea A, 22,99% na ?rea B e 9,80% na ?rea C. Nestas, foi realizada a extra??o de DNA, seguida de amplifica??o e sequenciamento para o alvo 18S. Sendo que destas 81, 53 amostras amplificaram na Nested-PCR. Posteriormente, estas amostras foram purificadas e encaminhadas para o sequenciamento, seguido de an?lises filogen?ticas. Neste estudo, foram diagnosticadas duas esp?cies: Cryptosporidium meleagridis em 44 amostras (91,67%) e Cryptosporidum baileyi em quatro amostras (8,33%), ambas se apresentando mais estreitamente relacionadas ?s esp?cies intestinais. As sequ?ncias de sete amostras contendo nucleot?deos diferentes de Cryptosporidum foram depositadas no Genbank. At? o momento o relato deste pat?geno em pombos s? havia ocorrido em quatro pa?ses no mundo: Turquia, Ir?, China e Tail?ndia. Os dados obtidos neste estudo refletem preocupa??o em sa?de p?blica, sendo que a C. meleagridis pode apresentar potencial zoon?tico. Este estudo ? o primeiro registro no Brasil tendo o hospedeiro pombo (Columba livia) infectado por Cryptosporidium, contribuindo para tra?ar a epidemiologia molecular deste agente etiol?gico no pa?sSubmitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2022-08-05T14:05:00Z No. of bitstreams: 1 2017 - Alexandre de Pina Costa.pdf: 6204092 bytes, checksum: 5fe06230edad5ee30b068a21f4ca8995 (MD5)Made available in DSpace on 2022-08-05T14:05:00Z (GMT). No. of bitstreams: 1 2017 - Alexandre de Pina Costa.pdf: 6204092 bytes, checksum: 5fe06230edad5ee30b068a21f4ca8995 (MD5) Previous issue date: 2017-03-09CAPES - Coordena??o de Aperfei?oamento de Pessoal de N?vel Superiorapplication/pdfhttps://tede.ufrrj.br/retrieve/70160/2017%20-%20Alexandre%20de%20Pina%20Costa.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Ci?ncias Veterin?riasUFRRJBrasilInstituto de Veterin?riaABAL-FABEIRO, J.L.; MASIDE, X.; BELLO, X.; LLOVO, J.; BARTOLOME, C. 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dc.title.por.fl_str_mv A import?ncia do pombo urbano (Columba livia) na dissemina??o ambiental de Cryptosporidium spp
dc.title.alternative.eng.fl_str_mv The importance of urban pigeon (Columba livia) on the Cryptosporidium spp. environment dissemination
title A import?ncia do pombo urbano (Columba livia) na dissemina??o ambiental de Cryptosporidium spp
spellingShingle A import?ncia do pombo urbano (Columba livia) na dissemina??o ambiental de Cryptosporidium spp
Costa, Alexandre de Pina
Sa?de p?blica
Diagn?stico molecular
Cryptosporidum meleagridis
Cryptosporidum baileyi
Contamina??o ambiental
Public health
molecular diagnosis
Cryptosporidum meleagridis
Cryptosporidum baileyi
environmental contamination
Medicina Veterin?ria
title_short A import?ncia do pombo urbano (Columba livia) na dissemina??o ambiental de Cryptosporidium spp
title_full A import?ncia do pombo urbano (Columba livia) na dissemina??o ambiental de Cryptosporidium spp
title_fullStr A import?ncia do pombo urbano (Columba livia) na dissemina??o ambiental de Cryptosporidium spp
title_full_unstemmed A import?ncia do pombo urbano (Columba livia) na dissemina??o ambiental de Cryptosporidium spp
title_sort A import?ncia do pombo urbano (Columba livia) na dissemina??o ambiental de Cryptosporidium spp
author Costa, Alexandre de Pina
author_facet Costa, Alexandre de Pina
author_role author
dc.contributor.advisor1.fl_str_mv Bomfim, Teresa Cristina Bergamo do
dc.contributor.advisor1ID.fl_str_mv 609.928.087-15
https://orcid.org/0000-0002-6741-0202
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/6299581965425007
dc.contributor.referee1.fl_str_mv Bomfim, Teresa Cristina Bergamo do
dc.contributor.referee1ID.fl_str_mv 609.928.087-15
https://orcid.org/0000-0002-6741-0202
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/6299581965425007
dc.contributor.referee2.fl_str_mv Sudr? , Adriana Pittella
dc.contributor.referee2ID.fl_str_mv https://orcid.org/0000-0002-0386-6278
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/1820344065898657
dc.contributor.referee3.fl_str_mv Santos, Helena L?cia Carneiro
dc.contributor.referee3ID.fl_str_mv https://orcid.org/0000-0002-5240-5662
dc.contributor.referee3Lattes.fl_str_mv http://lattes.cnpq.br/4847592947227424
dc.contributor.referee4.fl_str_mv Toma, Helena Keiko
dc.contributor.referee4Lattes.fl_str_mv http://lattes.cnpq.br/8121311753666435
dc.contributor.referee5.fl_str_mv Barros, Luciano Antunes
dc.contributor.referee5Lattes.fl_str_mv http://lattes.cnpq.br/8336187135982826
dc.contributor.authorID.fl_str_mv 032.513.387-57
https://orcid.org/0000-0002-4228-5118
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/7288645391409152
dc.contributor.author.fl_str_mv Costa, Alexandre de Pina
contributor_str_mv Bomfim, Teresa Cristina Bergamo do
Bomfim, Teresa Cristina Bergamo do
Sudr? , Adriana Pittella
Santos, Helena L?cia Carneiro
Toma, Helena Keiko
Barros, Luciano Antunes
dc.subject.por.fl_str_mv Sa?de p?blica
Diagn?stico molecular
Cryptosporidum meleagridis
Cryptosporidum baileyi
Contamina??o ambiental
topic Sa?de p?blica
Diagn?stico molecular
Cryptosporidum meleagridis
Cryptosporidum baileyi
Contamina??o ambiental
Public health
molecular diagnosis
Cryptosporidum meleagridis
Cryptosporidum baileyi
environmental contamination
Medicina Veterin?ria
dc.subject.eng.fl_str_mv Public health
molecular diagnosis
Cryptosporidum meleagridis
Cryptosporidum baileyi
environmental contamination
dc.subject.cnpq.fl_str_mv Medicina Veterin?ria
description The urban pigeon (Columba livia) is a bird frequently observed in urban centers, living in close contact with humans, being a possible disseminator of pathogens in the environment. Among these, special attention should be given to the species of Cryptosporidium, since the pigeon can harbor and eliminate the oocysts through the faeces. To date, three species are considered valid parasitizing birds (Cryptosporidium meleagridis, Cryptosporidium baileyi and Cryptosporidium galli) and 13 genotypes as possible agents of avian cryptosporidiosis. As the first species, besides parasitizing the birds class, has been diagnosed in several mammalian hosts, also zoonotic. Due to the lack of studies on the diagnosis of Cryptosporidium spp. parasitizing pigeons in Brazil and with few reports in the world, the present work had as objectives: to diagnose microscopically the presence of oocysts of Cryptosporidium spp. from pigeons faecal samples captured in three areas in the Rio de Janeiro city; to characterize genotypes and / or genotypes and / or subtypes obtained from those positive samples for Cryptosporidium spp. and perform sequencing and phylogenetic analyzes by comparing the sequences obtained with those sequences deposited in GenBank. A total of 387 pigeons fecal samples were collected in three administrative regions: Campo Grande - denominated A (98 samples), Bangu - denominated B (187 samples) and Madureira - denominated C (102 samples). Initially the Cryptosporidium spp. oocysts were investigated. For this, the faeces were processed through the technique of centrifugation and flotation in saturated sugar solution and evaluated by light microscopy. In the microscopic diagnosis, 81 (20.93%) samples were positive for the presence of Cryptosporidium spp. oocysts, distributed: 28.82% in area A, 22.99% in area B and 9.80% in area C. In these, DNA extraction was performed, followed by amplification and sequencing for the 18S target. Of these 81, 53 samples amplified in Nested-PCR. Subsequently, these samples were purified and sent for sequencing, followed by phylogenetic analyzes. In this study, two species were diagnosed: Cryptosporidium meleagridis in 44 samples (91.67%) and Cryptosporidum baileyi in four samples (8.33%), both of which were more closely related to intestinal species. Sequences of seven samples containing different nucleotides of Cryptosporidum were deposited in Genbank. So far, the report of this pathogen in pigeons had only occurred in four countries in the world: Turkey, Iran, China and Thailand. The data obtained in this study reflect public health concern, and C. meleagridis may present zoonotic potential. This study is the first Brazilian registry to have the host pigeon (Columba livia) infected by Cryptosporidium, contributing to trace the molecular epidemiology of this etiological agent in the country
publishDate 2017
dc.date.issued.fl_str_mv 2017-03-09
dc.date.accessioned.fl_str_mv 2022-08-05T14:05:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv COSTA, Alexandre de Pina. A import?ncia do pombo urbano (Columba livia) na dissemina??o ambiental de Cryptosporidium spp. 2017. 96 f. Tese (Doutorado em Ci?ncias Veterin?riasa) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2017
dc.identifier.uri.fl_str_mv https://tede.ufrrj.br/jspui/handle/jspui/5850
identifier_str_mv COSTA, Alexandre de Pina. A import?ncia do pombo urbano (Columba livia) na dissemina??o ambiental de Cryptosporidium spp. 2017. 96 f. Tese (Doutorado em Ci?ncias Veterin?riasa) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2017
url https://tede.ufrrj.br/jspui/handle/jspui/5850
dc.language.iso.fl_str_mv por
language por
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Anu?rio estat?stico. Bras?lia: IBGE, 2016. Dispon?vel em: < http://www.ibge.gov.br>. Acesso em: 13 de julho de 2016. IGNATIUS, R.; KLEMM, T.; ZANDER, S.; GAHUTU, J.B.; KIMMIG, P.; MOCKENHAUPT, F.P.; REGNATH, T. Highly specific detection of Cryptosporidium spp. oocysts in human stool samples by undemanding and inexpensive phase c
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