Efic?cia in vitro de diferentes formula??es de Metarhizium anisopliae s.l. no controle do carrapato Rhipicephalus microplus

Detalhes bibliográficos
Autor(a) principal: Nogueira, Michel Ruan dos Santos
Data de Publicação: 2014
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://tede.ufrrj.br/jspui/handle/jspui/4244
Resumo: Rhipicephalus microplus is responsible for causing severe damage to agribusiness. The development of alternative control methods is important to minimize the effects caused by the indiscriminate use of chemical acaricides. Biological control, using arthropodopathogenic fungi, is a promising option to maintain acceptable levels of tick populations in cattle. Currently, there are commercial fungal products with recognized insecticides effects and potential to be used for ticks control. Therefore, the present study evaluated the in vitro efficacy of two different products (Metarril SP Organic and Metarril SC Organic - Koppert Biological Systems?) for eggs, larvae and engorged females of R. microplus. Thirteen groups were formed: four aqueous suspensions Metarril SP Organic at concentrations of 106, 107, 108 and 109 conidia/mL; four oil formulations Metarril SC Organic in the same concentrations; four oil groups, compounds by the original concentration of the manufacturer (control 4) and three decimal dilutions of 10 (control 3), 100 (control 2) and 1000 (control 1) times and one group aqueous (sterile distilled water and Tween 80). Each group contained ten females, were immersed for three minutes in the suspensions/formulations. In biological assay with engorged females, periods of pre-laying, laying, incubation, hatching and percentage of hatching were observed; indexes: egg production and nutrition; reproductive efficiency and the percentage of control. The experiment with eggs was evaluated the hatching percentage, periods of incubation and hatching. Biological assay with larvae was evaluated the mortality percentage on different days. The test with engorged females Metarril SP Organic significantly reduced only the period of oviposition, at concentration of 109conidia/mL, reaching maximum control percentage of 28.6% in comparison with aqueous control. However, Metarril SC Organic was able to significantly reduce the period of oviposition, egg production index and nutrient index, presenting control percentage of 98.43% and 98.40% at concentration of 109 conidia/mL, in comparison to aqueous and oil 4 control, respectively. The bioassay eggs, Metarril SP Organic 109 conidia/mL and Metarril SC Organic 108 conidia/mL, were able to reduce the percentage of egg hatch, the results were 36,0% and 20,0%, respectively. The other parameters evaluated on this stage were reduced, but not significant. On the twentieth day after treatment of larvae, the product Metarril SP Organic concentrations 108 and 109 conidia/mL, the percentage mortality of 74,80% and 97,50%. However, Metarril SC Organic the same concentrations above 100% mortality achieved on the tenth day after treatment. The original presentation of the oily control demonstrated deleterious effect for all the evolutionary stage of R. microplus. The results of study showed that the tested products have the potential to be used in the in vitro control of different stages of R. microplus, especially Metarril SC Organic providing expectations for further studies.
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spelling Bittencourt, V?nia Rita Elias Pinheiro505198676-53http://lattes.cnpq.br/3888832724995864Perinotto, Wendell Marcelo de SouzaSantos, Regina Silva dosAngelo, Isabele Costa016325106-18http://lattes.cnpq.br/6978085183253041Nogueira, Michel Ruan dos Santos2020-12-08T10:26:16Z2014-09-24Nogueira, Michel Ruan dos Santos. Efic?cia in vitro de diferentes formula??es de Metarhizium anisopliae s.l. no controle do carrapato Rhipicephalus microplus. 2014. [45 f.]. Disserta??o( Programa de P?s-Gradua??o em Ci?ncias Veterin?rias) - Universidade Federal Rural do Rio de Janeiro, [Serop?dica - RJ] .https://tede.ufrrj.br/jspui/handle/jspui/4244Rhipicephalus microplus is responsible for causing severe damage to agribusiness. The development of alternative control methods is important to minimize the effects caused by the indiscriminate use of chemical acaricides. Biological control, using arthropodopathogenic fungi, is a promising option to maintain acceptable levels of tick populations in cattle. Currently, there are commercial fungal products with recognized insecticides effects and potential to be used for ticks control. Therefore, the present study evaluated the in vitro efficacy of two different products (Metarril SP Organic and Metarril SC Organic - Koppert Biological Systems?) for eggs, larvae and engorged females of R. microplus. Thirteen groups were formed: four aqueous suspensions Metarril SP Organic at concentrations of 106, 107, 108 and 109 conidia/mL; four oil formulations Metarril SC Organic in the same concentrations; four oil groups, compounds by the original concentration of the manufacturer (control 4) and three decimal dilutions of 10 (control 3), 100 (control 2) and 1000 (control 1) times and one group aqueous (sterile distilled water and Tween 80). Each group contained ten females, were immersed for three minutes in the suspensions/formulations. In biological assay with engorged females, periods of pre-laying, laying, incubation, hatching and percentage of hatching were observed; indexes: egg production and nutrition; reproductive efficiency and the percentage of control. The experiment with eggs was evaluated the hatching percentage, periods of incubation and hatching. Biological assay with larvae was evaluated the mortality percentage on different days. The test with engorged females Metarril SP Organic significantly reduced only the period of oviposition, at concentration of 109conidia/mL, reaching maximum control percentage of 28.6% in comparison with aqueous control. However, Metarril SC Organic was able to significantly reduce the period of oviposition, egg production index and nutrient index, presenting control percentage of 98.43% and 98.40% at concentration of 109 conidia/mL, in comparison to aqueous and oil 4 control, respectively. The bioassay eggs, Metarril SP Organic 109 conidia/mL and Metarril SC Organic 108 conidia/mL, were able to reduce the percentage of egg hatch, the results were 36,0% and 20,0%, respectively. The other parameters evaluated on this stage were reduced, but not significant. On the twentieth day after treatment of larvae, the product Metarril SP Organic concentrations 108 and 109 conidia/mL, the percentage mortality of 74,80% and 97,50%. However, Metarril SC Organic the same concentrations above 100% mortality achieved on the tenth day after treatment. The original presentation of the oily control demonstrated deleterious effect for all the evolutionary stage of R. microplus. The results of study showed that the tested products have the potential to be used in the in vitro control of different stages of R. microplus, especially Metarril SC Organic providing expectations for further studies.O carrapato Rhipicephalus microplus ? respons?vel por causar severos preju?zos ao agroneg?cio. Alternativas de controle s?o importantes para minimizar os efeitos causados pelo uso indiscriminado de acaricidas qu?micos. Assim, fungos artropodopatog?nicos tornam-se promissores para a manuten??o de n?veis aceit?veis da popula??o de carrapatos nos bovinos. Atualmente, existem no mercado produtos f?ngicos com efeito inseticida reconhecido e com potencial para utiliza??o no controle de carrapatos. Baseado nisso, o presente estudo avaliou a efic?cia in vitro de Metarril SP Organic e Metarril SC Organic (Koppert Biological Systems?) para ovos, larvas e f?meas ingurgitadas de R. microplus. Os experimentos foram compostos por 13 grupos: quatro suspens?es aquosas de Metarril SP Organic nas concentra??es 106, 107, 108 e 109 con?dios/mL; quatro formula??es oleosas de Metarril SC Organic nas mesmas concentra??es supracitadas; quatro grupos controle oleosos tratados com a concentra??o original do ?leo vegetal emulsific?vel (oleoso 4) e com tr?s dilui??es seriadas de 10 (oleoso 3), 100 (oleoso 2) e 1000 (oleoso 1) vezes e um grupo controle aquoso. Cada grupo continha dez f?meas, que foram imersas por tr?s minutos nas respectivas suspens?es/formula??es. No bioensaio com f?meas ingurgitadas, observou-se os per?odos de pr?-postura, postura, incuba??o, eclos?o e percentual de eclos?o, os ?ndices de produ??o de ovos e nutricional, a efici?ncia reprodutiva e o percentual de controle. No experimento com ovos avaliou-se o percentual de eclos?o e os per?odos de incuba??o e eclos?o. No bioensaio com larvas avaliou-se o percentual de mortalidade em diferentes dias. A normalidade dos dados foi analisada. Para os dados param?tricos foi feita An?lise de Vari?ncia seguida pelo teste de Tukey, para os n?o param?tricos foi utilizado o teste de Kruskal Wallis, seguido pelo teste de Dunn, verificados com n?vel de signific?ncia de 5%. No ensaio com f?meas ingurgitadas, Metarril SP Organic reduziu significativamente somente o per?odo de postura na concentra??o de 109 con?dios/mL, registrando percentual de controle de 28,6% em compara??o ao controle aquoso. Entretanto, Metarril SC Organic foi capaz de reduzir significativamente o per?odo de postura, ?ndices de produ??o de ovos e nutricional, apresentando percentual de controle de 98,43% e 94,90%, na concentra??o de 109 con?dios/mL, quando comparados aos controles aquoso e oleoso 4, respectivamente. No bioensaio com ovos, Metarril SP Organic a 109 con?dos/mL e Metarril SC Organic na concentra??o 108 con?dos/mL reduziram o percentual de eclos?o de larvas em 4,9 e 2,7 vezes, respectivamente. Nos outros par?metros avaliados sobre este est?dio houve redu??o, por?m n?o significativa. No experimento com larvas, no vig?simo dia ap?s o tratamento, o produto Metarril SP Organic nas concentra??es 108 e 109 con?dios/mL apresentou percentual de mortalidade de 74,80% e 97,50%, respectivamente. J? o produto Metarril SC Organic nas mesmas concentra??es supracitadas alcan?ou 100% de mortalidade no d?cimo dia ap?s o tratamento. A apresenta??o original do controle oleoso demonstrou efeito delet?rio para todas os est?gios evolutivos de R. microplus. Os resultados do estudo revelaram que os produtos testados possuem potencial para serem utilizados no controle in vitro dos diferentes est?gios de R. microplus, principalmente Metarril SC Organic proporcionando expectativas para futuros estudos.Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2020-12-08T10:26:16Z No. of bitstreams: 1 2014 - Michel Ruan dos Santos Nogueira.pdf: 1539753 bytes, checksum: f114cf806e937b3433efc4d951ed10fb (MD5)Made available in DSpace on 2020-12-08T10:26:16Z (GMT). No. of bitstreams: 1 2014 - Michel Ruan dos Santos Nogueira.pdf: 1539753 bytes, checksum: f114cf806e937b3433efc4d951ed10fb (MD5) Previous issue date: 2014-09-24Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior, CAPES, Brasil.application/pdfhttps://tede.ufrrj.br/retrieve/63414/2014%20-%20Michel%20Ruan%20dos%20Santos%20Nogueira.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Ci?ncias Veterin?riasUFRRJBrasilInstituto de Veterin?riaABBAS, R. 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dc.title.por.fl_str_mv Efic?cia in vitro de diferentes formula??es de Metarhizium anisopliae s.l. no controle do carrapato Rhipicephalus microplus
dc.title.alternative.eng.fl_str_mv In vitro efficacy of different formulations of Metarhizium anisopliae s.l. in control of Rhipicephalus microplus
title Efic?cia in vitro de diferentes formula??es de Metarhizium anisopliae s.l. no controle do carrapato Rhipicephalus microplus
spellingShingle Efic?cia in vitro de diferentes formula??es de Metarhizium anisopliae s.l. no controle do carrapato Rhipicephalus microplus
Nogueira, Michel Ruan dos Santos
controle biol?gico
fungos artropodopatog?nicos
formula??es f?ngicas
carrapatos
biological control
arthropodopathogenic fungi
fungal formulations
ticks
Medicina Veterin?ria
title_short Efic?cia in vitro de diferentes formula??es de Metarhizium anisopliae s.l. no controle do carrapato Rhipicephalus microplus
title_full Efic?cia in vitro de diferentes formula??es de Metarhizium anisopliae s.l. no controle do carrapato Rhipicephalus microplus
title_fullStr Efic?cia in vitro de diferentes formula??es de Metarhizium anisopliae s.l. no controle do carrapato Rhipicephalus microplus
title_full_unstemmed Efic?cia in vitro de diferentes formula??es de Metarhizium anisopliae s.l. no controle do carrapato Rhipicephalus microplus
title_sort Efic?cia in vitro de diferentes formula??es de Metarhizium anisopliae s.l. no controle do carrapato Rhipicephalus microplus
author Nogueira, Michel Ruan dos Santos
author_facet Nogueira, Michel Ruan dos Santos
author_role author
dc.contributor.advisor1.fl_str_mv Bittencourt, V?nia Rita Elias Pinheiro
dc.contributor.advisor1ID.fl_str_mv 505198676-53
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/3888832724995864
dc.contributor.advisor-co1.fl_str_mv Perinotto, Wendell Marcelo de Souza
dc.contributor.referee1.fl_str_mv Santos, Regina Silva dos
dc.contributor.referee2.fl_str_mv Angelo, Isabele Costa
dc.contributor.authorID.fl_str_mv 016325106-18
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/6978085183253041
dc.contributor.author.fl_str_mv Nogueira, Michel Ruan dos Santos
contributor_str_mv Bittencourt, V?nia Rita Elias Pinheiro
Perinotto, Wendell Marcelo de Souza
Santos, Regina Silva dos
Angelo, Isabele Costa
dc.subject.por.fl_str_mv controle biol?gico
fungos artropodopatog?nicos
formula??es f?ngicas
carrapatos
topic controle biol?gico
fungos artropodopatog?nicos
formula??es f?ngicas
carrapatos
biological control
arthropodopathogenic fungi
fungal formulations
ticks
Medicina Veterin?ria
dc.subject.eng.fl_str_mv biological control
arthropodopathogenic fungi
fungal formulations
ticks
dc.subject.cnpq.fl_str_mv Medicina Veterin?ria
description Rhipicephalus microplus is responsible for causing severe damage to agribusiness. The development of alternative control methods is important to minimize the effects caused by the indiscriminate use of chemical acaricides. Biological control, using arthropodopathogenic fungi, is a promising option to maintain acceptable levels of tick populations in cattle. Currently, there are commercial fungal products with recognized insecticides effects and potential to be used for ticks control. Therefore, the present study evaluated the in vitro efficacy of two different products (Metarril SP Organic and Metarril SC Organic - Koppert Biological Systems?) for eggs, larvae and engorged females of R. microplus. Thirteen groups were formed: four aqueous suspensions Metarril SP Organic at concentrations of 106, 107, 108 and 109 conidia/mL; four oil formulations Metarril SC Organic in the same concentrations; four oil groups, compounds by the original concentration of the manufacturer (control 4) and three decimal dilutions of 10 (control 3), 100 (control 2) and 1000 (control 1) times and one group aqueous (sterile distilled water and Tween 80). Each group contained ten females, were immersed for three minutes in the suspensions/formulations. In biological assay with engorged females, periods of pre-laying, laying, incubation, hatching and percentage of hatching were observed; indexes: egg production and nutrition; reproductive efficiency and the percentage of control. The experiment with eggs was evaluated the hatching percentage, periods of incubation and hatching. Biological assay with larvae was evaluated the mortality percentage on different days. The test with engorged females Metarril SP Organic significantly reduced only the period of oviposition, at concentration of 109conidia/mL, reaching maximum control percentage of 28.6% in comparison with aqueous control. However, Metarril SC Organic was able to significantly reduce the period of oviposition, egg production index and nutrient index, presenting control percentage of 98.43% and 98.40% at concentration of 109 conidia/mL, in comparison to aqueous and oil 4 control, respectively. The bioassay eggs, Metarril SP Organic 109 conidia/mL and Metarril SC Organic 108 conidia/mL, were able to reduce the percentage of egg hatch, the results were 36,0% and 20,0%, respectively. The other parameters evaluated on this stage were reduced, but not significant. On the twentieth day after treatment of larvae, the product Metarril SP Organic concentrations 108 and 109 conidia/mL, the percentage mortality of 74,80% and 97,50%. However, Metarril SC Organic the same concentrations above 100% mortality achieved on the tenth day after treatment. The original presentation of the oily control demonstrated deleterious effect for all the evolutionary stage of R. microplus. The results of study showed that the tested products have the potential to be used in the in vitro control of different stages of R. microplus, especially Metarril SC Organic providing expectations for further studies.
publishDate 2014
dc.date.issued.fl_str_mv 2014-09-24
dc.date.accessioned.fl_str_mv 2020-12-08T10:26:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv Nogueira, Michel Ruan dos Santos. Efic?cia in vitro de diferentes formula??es de Metarhizium anisopliae s.l. no controle do carrapato Rhipicephalus microplus. 2014. [45 f.]. Disserta??o( Programa de P?s-Gradua??o em Ci?ncias Veterin?rias) - Universidade Federal Rural do Rio de Janeiro, [Serop?dica - RJ] .
dc.identifier.uri.fl_str_mv https://tede.ufrrj.br/jspui/handle/jspui/4244
identifier_str_mv Nogueira, Michel Ruan dos Santos. Efic?cia in vitro de diferentes formula??es de Metarhizium anisopliae s.l. no controle do carrapato Rhipicephalus microplus. 2014. [45 f.]. Disserta??o( Programa de P?s-Gradua??o em Ci?ncias Veterin?rias) - Universidade Federal Rural do Rio de Janeiro, [Serop?dica - RJ] .
url https://tede.ufrrj.br/jspui/handle/jspui/4244
dc.language.iso.fl_str_mv por
language por
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