Modelo animal para a pesquisa dos potenciais evocados auditivos corticais
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | |
Tipo de documento: | Trabalho de conclusão de curso |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFS |
Texto Completo: | https://ri.ufs.br/jspui/handle/riufs/18019 |
Resumo: | Introduction: An electrical activity that occurs in the auditory system, generated by sound or electrical stimuli, is called Auditory Evoked Potential (AEP), they are visualized in the register as waves and are generated by different structures of the auditory system. Some experimental studies that have already been carried out have shown similarity between AEP generators in humans and animals. As their generators are multiple cortical areas, AEPs can be used to monitor neurophysiological changes including the impact of pharmacological agents on cortical functions. Purpose: to show the effectiveness of collecting Cortical Auditory Evoked Potentials in rats. Method: The CAEP, P1-N1-P2 complex, were collected through electrodes positioned on the skull, in the fronto-coronal (active), right mastoid (reference) and left mastoid (ground) regions in Rattus norvegicus, Wistar variety that were kept in cages with temperature, humidity and diet controlled in a cycle of 12 hours light and 12 hours dark, which were lightly sedated by intramuscular injection of Ketamine (0.1 ml) and Xylazine (0.1 ml) for collection of PEAs. Result: It was possible to collect the P1-N1-P2 complex, whose mean values (± standard deviation) were 73-198ms for latency and -2.9 ms to 2 ms for amplitude, respectively. Conclusion: It is possible to collect CAEP, more precisely the P1-N1-P2 complex in Rattus norvegicus, Wistar variety and use this animal as an experimental model for experiments on the auditory system. |
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Santos, Eva Maria Lima dosMello, Luanna Lennon NevesDuarte, Josilene Luciene2023-08-04T13:24:02Z2023-08-04T13:24:02Z2023-06-05SANTOS, Eva Maria Lima dos; MELLO, Luanna Lennon Neves. Modelo animal para a pesquisa dos potenciais evocados auditivos corticais. 2023. Trabalho de Conclusão de Curso (Graduação em Fonoaudiologia) - Universidade Federal de Sergipe, Lagarto, 2023.https://ri.ufs.br/jspui/handle/riufs/18019Introduction: An electrical activity that occurs in the auditory system, generated by sound or electrical stimuli, is called Auditory Evoked Potential (AEP), they are visualized in the register as waves and are generated by different structures of the auditory system. Some experimental studies that have already been carried out have shown similarity between AEP generators in humans and animals. As their generators are multiple cortical areas, AEPs can be used to monitor neurophysiological changes including the impact of pharmacological agents on cortical functions. Purpose: to show the effectiveness of collecting Cortical Auditory Evoked Potentials in rats. Method: The CAEP, P1-N1-P2 complex, were collected through electrodes positioned on the skull, in the fronto-coronal (active), right mastoid (reference) and left mastoid (ground) regions in Rattus norvegicus, Wistar variety that were kept in cages with temperature, humidity and diet controlled in a cycle of 12 hours light and 12 hours dark, which were lightly sedated by intramuscular injection of Ketamine (0.1 ml) and Xylazine (0.1 ml) for collection of PEAs. Result: It was possible to collect the P1-N1-P2 complex, whose mean values (± standard deviation) were 73-198ms for latency and -2.9 ms to 2 ms for amplitude, respectively. Conclusion: It is possible to collect CAEP, more precisely the P1-N1-P2 complex in Rattus norvegicus, Wistar variety and use this animal as an experimental model for experiments on the auditory system.Introdução: Uma atividade elétrica que ocorre no sistema auditivo, gerada por estímulos sonoros ou elétricos, é chamado de Potencial Evocado Auditivo (PEA), eles são visualizados no registro como ondas e são gerados por diferentes estruturas do sistema auditivo. Alguns estudos experimentais que já foram realizados mostraram similaridade entre os geradores dos PEAs em humanos e em animais. Como seus geradores são múltiplas áreas corticais, os PEAs podem ser utilizados para monitorar mudanças neurofisiológicas inclusive o impacto de agentes farmacológicos nas funções corticais. Objetivo: mostrar a efetividade da coleta de Potenciais Evocados Auditivos Corticais (PEACs) em ratos. Método: Foram coletados os PEACs, complexo P1-N1-P2, por meio de eletrodos posicionados no crânio, nas regiões fronto-coronal (ativo), Mastóide direita (referência) e mastóide esquerda (terra) em Rattus Norvegicus, variedade Wistar que foram mantidos em gaiolas com temperatura, umidade e dieta controladas em um ciclo de 12 horas claro e 12 horas escuro, que foram levemente sedados por meio de injeção intramuscular de Quetamina (0,1 ml) e Xilazina (0,1 ml) para a coleta dos PEAs. Resultado: Foi possível coletar o complexo P1-N1-P2, cujos valores médios (±15,7; 18,8 e 29,6) das latências e amplitude foram 73,4ms; 122,1 ms; 198,3 ms; e, 1,1 µV; 2,9 µV; 2,0 µV, respectivamente. Conclusão: É possível coletar os PEAC, mais precisamente o complexo P1-N1-P2 em Rattus norvegicus, variedade Wistar e utilizar este animal como modelo experimental para experimentos no sistema auditivo.LagartoporFonoaudiologiaRatos como animais de laboratórioModelos animais em pesquisaAudição (Fisiologia)Potenciais Evocados AuditivosRatos WistarHearingAuditory Evoked PotentialsWistar RatsAnimal ModelsModelo animal para a pesquisa dos potenciais evocados auditivos corticaisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisUniversidade Federal de Sergipe (UFS)DFOL - Departamento de Fonoaudiologia – Lagarto - Presencialreponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain; charset=utf-81475https://ri.ufs.br/jspui/bitstream/riufs/18019/1/license.txt098cbbf65c2c15e1fb2e49c5d306a44cMD51ORIGINALEva_Maria_Lima _dos_Santos&Luanna_Lennon_Neves_Mello-TCC.pdfEva_Maria_Lima _dos_Santos&Luanna_Lennon_Neves_Mello-TCC.pdfapplication/pdf334776https://ri.ufs.br/jspui/bitstream/riufs/18019/2/Eva_Maria_Lima%20_dos_Santos%26Luanna_Lennon_Neves_Mello-TCC.pdfbafe338162068e2b6df444357967bc05MD52TEXTEva_Maria_Lima _dos_Santos&Luanna_Lennon_Neves_Mello-TCC.pdf.txtEva_Maria_Lima _dos_Santos&Luanna_Lennon_Neves_Mello-TCC.pdf.txtExtracted texttext/plain29007https://ri.ufs.br/jspui/bitstream/riufs/18019/3/Eva_Maria_Lima%20_dos_Santos%26Luanna_Lennon_Neves_Mello-TCC.pdf.txt01c49e12e02a36fca65a096c43966befMD53THUMBNAILEva_Maria_Lima _dos_Santos&Luanna_Lennon_Neves_Mello-TCC.pdf.jpgEva_Maria_Lima _dos_Santos&Luanna_Lennon_Neves_Mello-TCC.pdf.jpgGenerated Thumbnailimage/jpeg1242https://ri.ufs.br/jspui/bitstream/riufs/18019/4/Eva_Maria_Lima%20_dos_Santos%26Luanna_Lennon_Neves_Mello-TCC.pdf.jpg4b773cd91fbbecfc87bea924026385b2MD54riufs/180192023-08-04 10:24:08.457oai:ufs.br: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Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2023-08-04T13:24:08Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false |
dc.title.pt_BR.fl_str_mv |
Modelo animal para a pesquisa dos potenciais evocados auditivos corticais |
title |
Modelo animal para a pesquisa dos potenciais evocados auditivos corticais |
spellingShingle |
Modelo animal para a pesquisa dos potenciais evocados auditivos corticais Santos, Eva Maria Lima dos Fonoaudiologia Ratos como animais de laboratório Modelos animais em pesquisa Audição (Fisiologia) Potenciais Evocados Auditivos Ratos Wistar Hearing Auditory Evoked Potentials Wistar Rats Animal Models |
title_short |
Modelo animal para a pesquisa dos potenciais evocados auditivos corticais |
title_full |
Modelo animal para a pesquisa dos potenciais evocados auditivos corticais |
title_fullStr |
Modelo animal para a pesquisa dos potenciais evocados auditivos corticais |
title_full_unstemmed |
Modelo animal para a pesquisa dos potenciais evocados auditivos corticais |
title_sort |
Modelo animal para a pesquisa dos potenciais evocados auditivos corticais |
author |
Santos, Eva Maria Lima dos |
author_facet |
Santos, Eva Maria Lima dos Mello, Luanna Lennon Neves |
author_role |
author |
author2 |
Mello, Luanna Lennon Neves |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Santos, Eva Maria Lima dos Mello, Luanna Lennon Neves |
dc.contributor.advisor1.fl_str_mv |
Duarte, Josilene Luciene |
contributor_str_mv |
Duarte, Josilene Luciene |
dc.subject.por.fl_str_mv |
Fonoaudiologia Ratos como animais de laboratório Modelos animais em pesquisa Audição (Fisiologia) Potenciais Evocados Auditivos Ratos Wistar |
topic |
Fonoaudiologia Ratos como animais de laboratório Modelos animais em pesquisa Audição (Fisiologia) Potenciais Evocados Auditivos Ratos Wistar Hearing Auditory Evoked Potentials Wistar Rats Animal Models |
dc.subject.eng.fl_str_mv |
Hearing Auditory Evoked Potentials Wistar Rats Animal Models |
description |
Introduction: An electrical activity that occurs in the auditory system, generated by sound or electrical stimuli, is called Auditory Evoked Potential (AEP), they are visualized in the register as waves and are generated by different structures of the auditory system. Some experimental studies that have already been carried out have shown similarity between AEP generators in humans and animals. As their generators are multiple cortical areas, AEPs can be used to monitor neurophysiological changes including the impact of pharmacological agents on cortical functions. Purpose: to show the effectiveness of collecting Cortical Auditory Evoked Potentials in rats. Method: The CAEP, P1-N1-P2 complex, were collected through electrodes positioned on the skull, in the fronto-coronal (active), right mastoid (reference) and left mastoid (ground) regions in Rattus norvegicus, Wistar variety that were kept in cages with temperature, humidity and diet controlled in a cycle of 12 hours light and 12 hours dark, which were lightly sedated by intramuscular injection of Ketamine (0.1 ml) and Xylazine (0.1 ml) for collection of PEAs. Result: It was possible to collect the P1-N1-P2 complex, whose mean values (± standard deviation) were 73-198ms for latency and -2.9 ms to 2 ms for amplitude, respectively. Conclusion: It is possible to collect CAEP, more precisely the P1-N1-P2 complex in Rattus norvegicus, Wistar variety and use this animal as an experimental model for experiments on the auditory system. |
publishDate |
2023 |
dc.date.accessioned.fl_str_mv |
2023-08-04T13:24:02Z |
dc.date.available.fl_str_mv |
2023-08-04T13:24:02Z |
dc.date.issued.fl_str_mv |
2023-06-05 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/bachelorThesis |
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bachelorThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SANTOS, Eva Maria Lima dos; MELLO, Luanna Lennon Neves. Modelo animal para a pesquisa dos potenciais evocados auditivos corticais. 2023. Trabalho de Conclusão de Curso (Graduação em Fonoaudiologia) - Universidade Federal de Sergipe, Lagarto, 2023. |
dc.identifier.uri.fl_str_mv |
https://ri.ufs.br/jspui/handle/riufs/18019 |
identifier_str_mv |
SANTOS, Eva Maria Lima dos; MELLO, Luanna Lennon Neves. Modelo animal para a pesquisa dos potenciais evocados auditivos corticais. 2023. Trabalho de Conclusão de Curso (Graduação em Fonoaudiologia) - Universidade Federal de Sergipe, Lagarto, 2023. |
url |
https://ri.ufs.br/jspui/handle/riufs/18019 |
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por |
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openAccess |
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Universidade Federal de Sergipe (UFS) |
dc.publisher.department.fl_str_mv |
DFOL - Departamento de Fonoaudiologia – Lagarto - Presencial |
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reponame:Repositório Institucional da UFS instname:Universidade Federal de Sergipe (UFS) instacron:UFS |
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UFS |
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Repositório Institucional da UFS |
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