Interleucina-10 e endotelina-1 como biomarcadores de doença valvar reumática
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Tipo de documento: | Trabalho de conclusão de curso |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFS |
Texto Completo: | http://ri.ufs.br/jspui/handle/riufs/7545 |
Resumo: | Objective: To evaluate the immunological profile and gene expression of endothelin-1 (ET-1) in mitral valves of patients with rheumatic fever originated from a reference service in cardiovascular surgery. Methods: This was a quantitative, observational and cross-sectional study. Thirty-five subjects (divided into four groups) participated in the study, 25 patients with chronic rheumatic heart disease and ten control subjects. The mean age of the sample studied was 34.5 years. Seventeen of them (48.58%) were male and 18 (51.42%) were female. Inflammatory cytokine IL-10 was measured and ten mitral valves of patients who underwent first valve replacement were collected for determination of gene expression of endothelin-1 by real time PCR. Results: Among the groups studied (patients vs. controls), there was a statistically significant difference in IL-10 levels (p=0.002). Expression of endothelin-1 was observed in 70% of samples. Quantitatively, average of ET-1 expression was 62.85±25.63%. Conclusion: Inflammatory cytokine IL-10 participates in the maintenance of chronicity of rheumatic fever in patients who underwent valve replacement and those who are undergoing medical treatment. The expression of endothelin-1 in heart valve lesions in patients undergoing mitral valve replacement confirms its association with inflammatory activity in rheumatic fever. |
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Fonseca, João Paulo AndradeRodrigues, Tania Maria de Andrade2018-03-16T17:42:57Z2018-03-16T17:42:57Z2018-02-08FONSECA, João Paulo Andrade. Interleucina-10 e endotelina-1 como biomarcadores de doença valvar reumática. Aracaju, SE, 2018. Monografia (Graduação em Medicina) - Departamento de Medicina, Universidade Federal de Sergipe, Aracaju, 2018.http://ri.ufs.br/jspui/handle/riufs/7545Objective: To evaluate the immunological profile and gene expression of endothelin-1 (ET-1) in mitral valves of patients with rheumatic fever originated from a reference service in cardiovascular surgery. Methods: This was a quantitative, observational and cross-sectional study. Thirty-five subjects (divided into four groups) participated in the study, 25 patients with chronic rheumatic heart disease and ten control subjects. The mean age of the sample studied was 34.5 years. Seventeen of them (48.58%) were male and 18 (51.42%) were female. Inflammatory cytokine IL-10 was measured and ten mitral valves of patients who underwent first valve replacement were collected for determination of gene expression of endothelin-1 by real time PCR. Results: Among the groups studied (patients vs. controls), there was a statistically significant difference in IL-10 levels (p=0.002). Expression of endothelin-1 was observed in 70% of samples. Quantitatively, average of ET-1 expression was 62.85±25.63%. Conclusion: Inflammatory cytokine IL-10 participates in the maintenance of chronicity of rheumatic fever in patients who underwent valve replacement and those who are undergoing medical treatment. The expression of endothelin-1 in heart valve lesions in patients undergoing mitral valve replacement confirms its association with inflammatory activity in rheumatic fever.Objetivo: Avaliar o perfil imunológico e a expressão gênica de endotelina-1 em valvas mitrais de pacientes com febre reumática, originados de um serviço de referência em cirurgia cardiovascular. Métodos: Este foi um estudo quantitativo, observacional e transversal. Trinta e cinco indivíduos (divididos em quatro grupos) participaram do estudo, 25 deles com doença cardíaca reumática crônica, além de 10 controles. A média de idade da amostra estudada foi de 34,5 anos. Dezessete (48,58%) dos indivíduos eram homens, e 18 (51,42%) eram mulheres. Mediu-se a citocina infamatória IL-10 e foram coletadas 10 valvas mitrais de pacientes que se submeteram a primeira troca valvar para determinação da expressão gênica de endotelina-1 pelo PCR real-time. Resultados: Entre os grupos estudados (pacientes e controles), observou-se diferença estatisticamente significante em relação aos níveis de IL-10 (p=0,002). Em relação à endotelina-1, foi observada sua expressão em 70% das amostras. Quantitativamente, a expressão média de endotelina-1 foi de 62,85±25,63%. Conclusão: A citocina inflamatória IL-10 participa da manutenção da cronicidade da febre reumática em pacientes que se submeteram a troca valvar e naqueles que estão em tratamento médico. A expressão de endotelina-1 nas lesões em valvas cardíacas de pacientes que foram submetidos à troca valvar mitral confirma sua relação com a atividade inflamatória na febre reumática.Aracaju, SEporMedicinaSaúdeFebre reumáticaEstenose da válvula mitralEndotelinasCélulas TMedicineRheumatic feverEndothelinCIENCIAS DA SAUDE::MEDICINAInterleucina-10 e endotelina-1 como biomarcadores de doença valvar reumáticaInterleukin-10 and endothelin-1 as biomarkers of rheumatic valve diseaseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisUniversidade Federal de SergipeDME - Departamento de Medicina – Aracaju - Presencialreponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain; charset=utf-81475https://ri.ufs.br/jspui/bitstream/riufs/7545/1/license.txt098cbbf65c2c15e1fb2e49c5d306a44cMD51ORIGINALJoão_ Paulo_ Andrade_ Fonseca.pdfJoão_ Paulo_ Andrade_ Fonseca.pdfapplication/pdf566845https://ri.ufs.br/jspui/bitstream/riufs/7545/2/Jo%c3%a3o_%20Paulo_%20Andrade_%20Fonseca.pdf33e37417b24b51e1a8eeab41325911f5MD52TEXTJoão_ Paulo_ Andrade_ Fonseca.pdf.txtJoão_ Paulo_ Andrade_ Fonseca.pdf.txtExtracted texttext/plain122734https://ri.ufs.br/jspui/bitstream/riufs/7545/3/Jo%c3%a3o_%20Paulo_%20Andrade_%20Fonseca.pdf.txt90a2caf7cd04bc62fe6898c6109616b5MD53THUMBNAILJoão_ Paulo_ Andrade_ Fonseca.pdf.jpgJoão_ Paulo_ Andrade_ Fonseca.pdf.jpgGenerated Thumbnailimage/jpeg1297https://ri.ufs.br/jspui/bitstream/riufs/7545/4/Jo%c3%a3o_%20Paulo_%20Andrade_%20Fonseca.pdf.jpg3c1bf51a2e219eb66562a4c50eb700c2MD54riufs/75452018-03-16 14:42:58.042oai:ufs.br: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Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2018-03-16T17:42:58Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false |
dc.title.pt_BR.fl_str_mv |
Interleucina-10 e endotelina-1 como biomarcadores de doença valvar reumática |
dc.title.alternative.eng.fl_str_mv |
Interleukin-10 and endothelin-1 as biomarkers of rheumatic valve disease |
title |
Interleucina-10 e endotelina-1 como biomarcadores de doença valvar reumática |
spellingShingle |
Interleucina-10 e endotelina-1 como biomarcadores de doença valvar reumática Fonseca, João Paulo Andrade Medicina Saúde Febre reumática Estenose da válvula mitral Endotelinas Células T Medicine Rheumatic fever Endothelin CIENCIAS DA SAUDE::MEDICINA |
title_short |
Interleucina-10 e endotelina-1 como biomarcadores de doença valvar reumática |
title_full |
Interleucina-10 e endotelina-1 como biomarcadores de doença valvar reumática |
title_fullStr |
Interleucina-10 e endotelina-1 como biomarcadores de doença valvar reumática |
title_full_unstemmed |
Interleucina-10 e endotelina-1 como biomarcadores de doença valvar reumática |
title_sort |
Interleucina-10 e endotelina-1 como biomarcadores de doença valvar reumática |
author |
Fonseca, João Paulo Andrade |
author_facet |
Fonseca, João Paulo Andrade |
author_role |
author |
dc.contributor.author.fl_str_mv |
Fonseca, João Paulo Andrade |
dc.contributor.advisor1.fl_str_mv |
Rodrigues, Tania Maria de Andrade |
contributor_str_mv |
Rodrigues, Tania Maria de Andrade |
dc.subject.por.fl_str_mv |
Medicina Saúde Febre reumática Estenose da válvula mitral Endotelinas Células T |
topic |
Medicina Saúde Febre reumática Estenose da válvula mitral Endotelinas Células T Medicine Rheumatic fever Endothelin CIENCIAS DA SAUDE::MEDICINA |
dc.subject.eng.fl_str_mv |
Medicine Rheumatic fever Endothelin |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::MEDICINA |
description |
Objective: To evaluate the immunological profile and gene expression of endothelin-1 (ET-1) in mitral valves of patients with rheumatic fever originated from a reference service in cardiovascular surgery. Methods: This was a quantitative, observational and cross-sectional study. Thirty-five subjects (divided into four groups) participated in the study, 25 patients with chronic rheumatic heart disease and ten control subjects. The mean age of the sample studied was 34.5 years. Seventeen of them (48.58%) were male and 18 (51.42%) were female. Inflammatory cytokine IL-10 was measured and ten mitral valves of patients who underwent first valve replacement were collected for determination of gene expression of endothelin-1 by real time PCR. Results: Among the groups studied (patients vs. controls), there was a statistically significant difference in IL-10 levels (p=0.002). Expression of endothelin-1 was observed in 70% of samples. Quantitatively, average of ET-1 expression was 62.85±25.63%. Conclusion: Inflammatory cytokine IL-10 participates in the maintenance of chronicity of rheumatic fever in patients who underwent valve replacement and those who are undergoing medical treatment. The expression of endothelin-1 in heart valve lesions in patients undergoing mitral valve replacement confirms its association with inflammatory activity in rheumatic fever. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-03-16T17:42:57Z |
dc.date.available.fl_str_mv |
2018-03-16T17:42:57Z |
dc.date.issued.fl_str_mv |
2018-02-08 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/bachelorThesis |
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bachelorThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
FONSECA, João Paulo Andrade. Interleucina-10 e endotelina-1 como biomarcadores de doença valvar reumática. Aracaju, SE, 2018. Monografia (Graduação em Medicina) - Departamento de Medicina, Universidade Federal de Sergipe, Aracaju, 2018. |
dc.identifier.uri.fl_str_mv |
http://ri.ufs.br/jspui/handle/riufs/7545 |
identifier_str_mv |
FONSECA, João Paulo Andrade. Interleucina-10 e endotelina-1 como biomarcadores de doença valvar reumática. Aracaju, SE, 2018. Monografia (Graduação em Medicina) - Departamento de Medicina, Universidade Federal de Sergipe, Aracaju, 2018. |
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por |
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por |
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openAccess |
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Universidade Federal de Sergipe |
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DME - Departamento de Medicina – Aracaju - Presencial |
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