Genetic diversity analysis of Varronia curassavica Jacq. accessions using ISSR markers

• Autor(a) principal: Brito, Fabiany de Andrade
• Data de Publicação: 2016
• Outros Autores: Nizio, Daniela Aparecida de Castro, Muniz, Ana Veruska Cruz da Silva, Diniz, Leandro Eugenio Cardamone, Rabbani, Allívia Rouse Carregosa, Arrigoni-Blank, Maria de Fátima, Carvalho, Sheila Valéria Álvares, Figueira, Glyn Mara, Montanari Junior, Ilio, Blank, Arie Fitzgerald
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFS
• Texto Completo: http://ri.ufs.br/jspui/handle/riufs/7286
• topic: Varronia curassavica; Conservation; Diversity; ISSR; Conservação; Diversidade

Varronia curassavica Jacq. is a medicinal and aromatic plant from Brazil with significant economic importance. Studies on genetic diversity in active germplasm banks (AGB) are essential for conservation and breeding programs. The aim of this study was to analyze the genetic diversity of V. curassavica accessions of the AGB of Medicinal and Aromatic Plants of the Federal University of Sergipe (UFS), using inter-simple sequence repeat molecular markers. Twentyfour primers were tested, and 14 were polymorphic and informative, resulting in 149 bands with 97.98% polymorphism. The UPGMA dendrogram divided the accessions into Clusters I and II. Jaccard similarity coefficients for pair-wise comparisons of accessions ranged between 0.24 and 0.78. The pairs of accessions VCUR-001/VCUR-503, VCUR-001/VCUR-504, and VCUR-104/VCUR-501 showed relatively low similarity (0.24), and the pair of accessions VCUR-402/VCUR403 showed medium similarity (0.78). Twenty-eight accessions were divided into three distinct clusters, according to the STRUCTURE analysis. The genetic diversity of V. curassavica in the AGB of UFS is low to medium, and it requires expansion. Accession VCUR-802 is the most suitable for selection in breeding program of this species, since it clearly represents all of the diversity present in the AGB.