Analysis of meiotic behavior in Cordia ecalyculata Vell. (Boraginaceae)

• Autor(a) principal: Nascimento, Damião do
• Data de Publicação: 2014
• Outros Autores: Sbais, Patricia Gonçalves, Alonso-Pereira, Andréia Rodrigues, Canto, Adriele Fialho de, Romagnolo, Mariza Barion, Risso-Pascotto, Claudiceia, Alberton, Odair
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Biotemas (Online)
• Texto Completo: https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2014v27n4p21

Cordia ecalyculata belongs to the Boraginaceae family, and is commonly known as buggy coffee. It is indicated for medicinal use as a tonic, diuretic, anti-inflammatory and appetite suppressant. Young inflorescences of six individuals were collected and fixed in a mixture of ethanol and acetic acid (3:1) for 24 hours. The slides were prepared by crushing and staining tissue with 1% acetic carmine. During microsporogenesis some irregularities were observed, mostly frequently related to irregular chromosome segregation. Irregularities included: precocious migration to poles in metaphase I and II, disoriented bivalent chromosomes at metaphase I and II, laggard chromosomes in anaphase I and II, and micronuclei formation. We also observed irregular spindle organization in meiosis II, leading to ‘T’ and ‘V’ shaped spindle configurations. In the V-shaped configuration, two nearby nuclei fused, forming triads instead of tetrads; this lead to formation of 2n microspore at the end of meiosis. However, pollen grain viability was not compromised, as pollen grain viability varied between 95.42% and 100%.