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Detalhes bibliográficos
Autor(a) principal: Stefenon, Valdir Marcos
Data de Publicação: 2004
Outros Autores: Nodari, Rubens Onofre, Guerra, Miguel Pedro
Tipo de documento: Artigo
Idioma: por
Título da fonte: Biotemas (Online)
Texto Completo: https://periodicos.ufsc.br/index.php/biotemas/article/view/23267
Resumo: This study was aimed at adapting a DNA extraction protocol by Araucaria angustifolia leaves, and testing the informative capacity of RAPD markers for genetics diversity analysis in natural populations of this species. The extraction method was standardized by eight tested protocols and it was possible to Obtain good quality DNA for RAPD reactions. The OD260/0D280 ratio ranged from 1.7 to 2.0 in 80% of the samples, indicating that they had a low level of protein contamination. The RAPD markers demonstrated their capacity as a useful tool to genetic studies, since the estimate of a genetic diversity (Shannon index H=0.50) higher than that obtained by other authors with isozyme markers (H=0.29) and PCR-RFLP (H=0.0) for the same natural population of the Ecological Municipal Park, Lages, Santa Catarina, Brazil.
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spelling .Genética e conservação de Araucaria angustifolia: III. Protocolo de extração de DNA e capacidade informativa de marcadores RAPD para análise da diversidade genética em populações naturaisThis study was aimed at adapting a DNA extraction protocol by Araucaria angustifolia leaves, and testing the informative capacity of RAPD markers for genetics diversity analysis in natural populations of this species. The extraction method was standardized by eight tested protocols and it was possible to Obtain good quality DNA for RAPD reactions. The OD260/0D280 ratio ranged from 1.7 to 2.0 in 80% of the samples, indicating that they had a low level of protein contamination. The RAPD markers demonstrated their capacity as a useful tool to genetic studies, since the estimate of a genetic diversity (Shannon index H=0.50) higher than that obtained by other authors with isozyme markers (H=0.29) and PCR-RFLP (H=0.0) for the same natural population of the Ecological Municipal Park, Lages, Santa Catarina, Brazil.Este trabalho teve como objetivo adaptar um protocolo para a extração de DNA a partir de aciculas de Araucaria angustifolia e testar a capacidade informativa de marcadores RAPD para a análise da diversidade genética de populações naturais desta espécie. 0 método de extração foi padronizado a partir de oito protocolos testados e permitiu a obtenção de DNA com boa qualidade para as reações de RAPD. A razão OD 260/0D280 de 80% das amostras apresentou um valor entre 1,7 e 2,0 indicando que estas apresentaram baixa contaminação por proteínas. Os marcadores RAPD demonstraram ser uma eficiente ferramenta para estudos genéticos, possibilitando a estimativa de um índice de diversidade genética (índice de Shannon H=0,50) superior ao obtido com marcadores isoenzimáticos (H=0,29) e PCR-RFLP (H=0,0), para a população natural de araucária do Parque Ecológico Municipal de Lages/SC.Universidade Federal de Santa Catarina2004-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsc.br/index.php/biotemas/article/view/23267Biotemas; v. 17 n. 1 (2004); 47-632175-79250103-1643reponame:Biotemas (Online)instname:Universidade Federal de Santa Catarina (UFSC)instacron:UFSCporhttps://periodicos.ufsc.br/index.php/biotemas/article/view/23267/21001Copyright (c) 2004 Valdir Marcos Stefenon, Rubens Onofre Nodari, Miguel Pedro Guerrahttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessStefenon, Valdir MarcosNodari, Rubens OnofreGuerra, Miguel Pedro2022-11-21T11:14:15Zoai:periodicos.ufsc.br:article/23267Revistahttp://www.biotemas.ufsc.br/index.htmPUBhttps://periodicos.ufsc.br/index.php/biotemas/oai||carlospinto@ccb.ufsc.br2175-79250103-1643opendoar:2022-11-21T11:14:15Biotemas (Online) - Universidade Federal de Santa Catarina (UFSC)false
dc.title.none.fl_str_mv .
Genética e conservação de Araucaria angustifolia: III. Protocolo de extração de DNA e capacidade informativa de marcadores RAPD para análise da diversidade genética em populações naturais
title .
spellingShingle .
Stefenon, Valdir Marcos
title_short .
title_full .
title_fullStr .
title_full_unstemmed .
title_sort .
author Stefenon, Valdir Marcos
author_facet Stefenon, Valdir Marcos
Nodari, Rubens Onofre
Guerra, Miguel Pedro
author_role author
author2 Nodari, Rubens Onofre
Guerra, Miguel Pedro
author2_role author
author
dc.contributor.author.fl_str_mv Stefenon, Valdir Marcos
Nodari, Rubens Onofre
Guerra, Miguel Pedro
description This study was aimed at adapting a DNA extraction protocol by Araucaria angustifolia leaves, and testing the informative capacity of RAPD markers for genetics diversity analysis in natural populations of this species. The extraction method was standardized by eight tested protocols and it was possible to Obtain good quality DNA for RAPD reactions. The OD260/0D280 ratio ranged from 1.7 to 2.0 in 80% of the samples, indicating that they had a low level of protein contamination. The RAPD markers demonstrated their capacity as a useful tool to genetic studies, since the estimate of a genetic diversity (Shannon index H=0.50) higher than that obtained by other authors with isozyme markers (H=0.29) and PCR-RFLP (H=0.0) for the same natural population of the Ecological Municipal Park, Lages, Santa Catarina, Brazil.
publishDate 2004
dc.date.none.fl_str_mv 2004-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.ufsc.br/index.php/biotemas/article/view/23267
url https://periodicos.ufsc.br/index.php/biotemas/article/view/23267
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.ufsc.br/index.php/biotemas/article/view/23267/21001
dc.rights.driver.fl_str_mv Copyright (c) 2004 Valdir Marcos Stefenon, Rubens Onofre Nodari, Miguel Pedro Guerra
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2004 Valdir Marcos Stefenon, Rubens Onofre Nodari, Miguel Pedro Guerra
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Catarina
publisher.none.fl_str_mv Universidade Federal de Santa Catarina
dc.source.none.fl_str_mv Biotemas; v. 17 n. 1 (2004); 47-63
2175-7925
0103-1643
reponame:Biotemas (Online)
instname:Universidade Federal de Santa Catarina (UFSC)
instacron:UFSC
instname_str Universidade Federal de Santa Catarina (UFSC)
instacron_str UFSC
institution UFSC
reponame_str Biotemas (Online)
collection Biotemas (Online)
repository.name.fl_str_mv Biotemas (Online) - Universidade Federal de Santa Catarina (UFSC)
repository.mail.fl_str_mv ||carlospinto@ccb.ufsc.br
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