Uso de imagens digitais e smartphone para determinação de eugenol em óleo essencial do cravo-da-india (Syzygium aromaticum)
Autor(a) principal: | |
---|---|
Data de Publicação: | 2023 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/29097 |
Resumo: | In this study, innovative and fast alternatives for determining the major constituents of clove essential oil were developed with digital images. The experiments were initially carried out using thin-layer chromatography (TLC) and, later, by colorimetry using a smartphone. The types of eluents, lighting, software, development methods, detection, and the influence of volume on the samples were previously optimized for TLC. A portable system was designed to acquire images based on the use of a smartphone, with subsequent quantification tests being made using the Photometrix app and other free software such as Image J and qTLC. The results were inaccurate due to the lack of reproducibility in analyses. Thus, colorimetry associated with the use of digital images in a smartphone was used for the determination of eugenol in clove oil. The Photometrix app was used to evaluate the colorimetric reaction between the eugenol and the Folin-Ciocalteu reagent. The results were in agreement with those obtained through traditional methodologies, such as gas chromatography and spectrophotometry, ranging from 97% to 101% for the five samples tested. The developed method allowed the analysis of several samples in a short period of time. Portability and ease of operation are also noteworthy, making it useful in the field and in places with restricted access. |
id |
UFSM-20_474daac843081f3a7e7f542619c95e8e |
---|---|
oai_identifier_str |
oai:repositorio.ufsm.br:1/29097 |
network_acronym_str |
UFSM-20 |
network_name_str |
Manancial - Repositório Digital da UFSM |
repository_id_str |
3913 |
spelling |
2023-05-16T13:19:05Z2023-05-16T13:19:05Z2023-03-17http://repositorio.ufsm.br/handle/1/29097In this study, innovative and fast alternatives for determining the major constituents of clove essential oil were developed with digital images. The experiments were initially carried out using thin-layer chromatography (TLC) and, later, by colorimetry using a smartphone. The types of eluents, lighting, software, development methods, detection, and the influence of volume on the samples were previously optimized for TLC. A portable system was designed to acquire images based on the use of a smartphone, with subsequent quantification tests being made using the Photometrix app and other free software such as Image J and qTLC. The results were inaccurate due to the lack of reproducibility in analyses. Thus, colorimetry associated with the use of digital images in a smartphone was used for the determination of eugenol in clove oil. The Photometrix app was used to evaluate the colorimetric reaction between the eugenol and the Folin-Ciocalteu reagent. The results were in agreement with those obtained through traditional methodologies, such as gas chromatography and spectrophotometry, ranging from 97% to 101% for the five samples tested. The developed method allowed the analysis of several samples in a short period of time. Portability and ease of operation are also noteworthy, making it useful in the field and in places with restricted access.No presente estudo, alternativas inovadoras e rápidas para a determinação de constituintes majoritários presentes em amostras de cravo-da-índia foram desenvolvidas com o auxílio de imagens digitais. Os experimentos foram realizados, inicialmente, por meio da Cromatografia em Camada Delgada (CCD) e, posteriormente, por Colorimetria empregando smartphone. Otimizou-se, previamente, para CCD os tipos de eluentes, iluminação, softwares, métodos de revelação, detecção, bem como a influência de volume nas amostras. Foi projetado um sistema portátil para a aquisição das imagens baseado no uso de smartphone e posterior ensaios de quantificação por meio do aplicativo Photometrix e outros softwares livres como ImageJ e qTLC. Respostas não precisas foram encontradas devido às dificuldades de reprodutibilidade das análises, inviabilizando a quantificação de eugenol. Assim, a Colorimetria associada ao uso de imagens digitais com smartphone foi usada para determinação de eugenol no óleo de cravo-da-índia. O smartphone, juntamente ao aplicativo Photometrix, foi empregado para avaliação da reação colorimétrica do eugenol com o reagente de Folin-Ciocalteu. Os resultados apresentaram concordância frente aos métodos tradicionais de análise como Cromatografia Gasosa e Espectrofotometria, variando de 97% a 101% para as cinco amostras testadas. O método desenvolvido permitiu a análise de diversas amostras em um curto período de tempo. Destaca-se, ainda, a portabilidade e a fácil operação, podendo ser útil para aplicações em campo e locais de acesso restrito.porUniversidade Federal de Santa MariaCentro de Ciências RuraisPrograma de Pós-Graduação em Ciência e Tecnologia dos AlimentosUFSMBrasilCiência e Tecnologia dos AlimentosAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessColorimetriaImagens digitaisDeterminação de eugenolÓleo essencial de cravo-da-índiaColorimetryDigital imagesEugenol determinationClove essential oilCNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOSUso de imagens digitais e smartphone para determinação de eugenol em óleo essencial do cravo-da-india (Syzygium aromaticum)Determination of eugenol in clove essential oil using a smartphoneinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisBarin, Juliano Smaniotohttp://lattes.cnpq.br/7545847424095994Giacomelli, Sandro RogérioBolzan, Rodrigo CordeiroDuarte, Fábio AndreiVoss, Mônicahttp://lattes.cnpq.br/3351377314764594Santos, Izaura Ceolin dos500700000006600600600600600600600f4cb9cf1-d105-4e6c-b0fa-acdf690e8cabcc13a368-7e79-407b-8acc-aec185bf546658a20221-13eb-4460-b8e9-2457cc8a11ff0b6036d2-a4c4-4565-bfc3-5fef0aae6060f60c61ec-2b88-485f-8d1e-83d62038970d10cfe646-7ab9-4eee-a351-713b0aae0e3breponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALTES_PPGCTA_2023_SANTOS_IZAURA.pdfTES_PPGCTA_2023_SANTOS_IZAURA.pdfTese de Doutoradoapplication/pdf1381843http://repositorio.ufsm.br/bitstream/1/29097/1/TES_PPGCTA_2023_SANTOS_IZAURA.pdf2792f9d2d1ff3ec3d03470ceb1185a00MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81956http://repositorio.ufsm.br/bitstream/1/29097/3/license.txt2f0571ecee68693bd5cd3f17c1e075dfMD53CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/29097/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD521/290972023-05-16 10:19:05.955oai:repositorio.ufsm.br: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ório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestopendoar:39132023-05-16T13:19:05Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Uso de imagens digitais e smartphone para determinação de eugenol em óleo essencial do cravo-da-india (Syzygium aromaticum) |
dc.title.alternative.eng.fl_str_mv |
Determination of eugenol in clove essential oil using a smartphone |
title |
Uso de imagens digitais e smartphone para determinação de eugenol em óleo essencial do cravo-da-india (Syzygium aromaticum) |
spellingShingle |
Uso de imagens digitais e smartphone para determinação de eugenol em óleo essencial do cravo-da-india (Syzygium aromaticum) Santos, Izaura Ceolin dos Colorimetria Imagens digitais Determinação de eugenol Óleo essencial de cravo-da-índia Colorimetry Digital images Eugenol determination Clove essential oil CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS |
title_short |
Uso de imagens digitais e smartphone para determinação de eugenol em óleo essencial do cravo-da-india (Syzygium aromaticum) |
title_full |
Uso de imagens digitais e smartphone para determinação de eugenol em óleo essencial do cravo-da-india (Syzygium aromaticum) |
title_fullStr |
Uso de imagens digitais e smartphone para determinação de eugenol em óleo essencial do cravo-da-india (Syzygium aromaticum) |
title_full_unstemmed |
Uso de imagens digitais e smartphone para determinação de eugenol em óleo essencial do cravo-da-india (Syzygium aromaticum) |
title_sort |
Uso de imagens digitais e smartphone para determinação de eugenol em óleo essencial do cravo-da-india (Syzygium aromaticum) |
author |
Santos, Izaura Ceolin dos |
author_facet |
Santos, Izaura Ceolin dos |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Barin, Juliano Smanioto |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/7545847424095994 |
dc.contributor.referee1.fl_str_mv |
Giacomelli, Sandro Rogério |
dc.contributor.referee2.fl_str_mv |
Bolzan, Rodrigo Cordeiro |
dc.contributor.referee3.fl_str_mv |
Duarte, Fábio Andrei |
dc.contributor.referee4.fl_str_mv |
Voss, Mônica |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3351377314764594 |
dc.contributor.author.fl_str_mv |
Santos, Izaura Ceolin dos |
contributor_str_mv |
Barin, Juliano Smanioto Giacomelli, Sandro Rogério Bolzan, Rodrigo Cordeiro Duarte, Fábio Andrei Voss, Mônica |
dc.subject.por.fl_str_mv |
Colorimetria Imagens digitais Determinação de eugenol Óleo essencial de cravo-da-índia |
topic |
Colorimetria Imagens digitais Determinação de eugenol Óleo essencial de cravo-da-índia Colorimetry Digital images Eugenol determination Clove essential oil CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS |
dc.subject.eng.fl_str_mv |
Colorimetry Digital images Eugenol determination Clove essential oil |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS |
description |
In this study, innovative and fast alternatives for determining the major constituents of clove essential oil were developed with digital images. The experiments were initially carried out using thin-layer chromatography (TLC) and, later, by colorimetry using a smartphone. The types of eluents, lighting, software, development methods, detection, and the influence of volume on the samples were previously optimized for TLC. A portable system was designed to acquire images based on the use of a smartphone, with subsequent quantification tests being made using the Photometrix app and other free software such as Image J and qTLC. The results were inaccurate due to the lack of reproducibility in analyses. Thus, colorimetry associated with the use of digital images in a smartphone was used for the determination of eugenol in clove oil. The Photometrix app was used to evaluate the colorimetric reaction between the eugenol and the Folin-Ciocalteu reagent. The results were in agreement with those obtained through traditional methodologies, such as gas chromatography and spectrophotometry, ranging from 97% to 101% for the five samples tested. The developed method allowed the analysis of several samples in a short period of time. Portability and ease of operation are also noteworthy, making it useful in the field and in places with restricted access. |
publishDate |
2023 |
dc.date.accessioned.fl_str_mv |
2023-05-16T13:19:05Z |
dc.date.available.fl_str_mv |
2023-05-16T13:19:05Z |
dc.date.issued.fl_str_mv |
2023-03-17 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/29097 |
url |
http://repositorio.ufsm.br/handle/1/29097 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
500700000006 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 600 600 600 |
dc.relation.authority.fl_str_mv |
f4cb9cf1-d105-4e6c-b0fa-acdf690e8cab cc13a368-7e79-407b-8acc-aec185bf5466 58a20221-13eb-4460-b8e9-2457cc8a11ff 0b6036d2-a4c4-4565-bfc3-5fef0aae6060 f60c61ec-2b88-485f-8d1e-83d62038970d 10cfe646-7ab9-4eee-a351-713b0aae0e3b |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Rurais |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciência e Tecnologia dos Alimentos |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Ciência e Tecnologia dos Alimentos |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Rurais |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
bitstream.url.fl_str_mv |
http://repositorio.ufsm.br/bitstream/1/29097/1/TES_PPGCTA_2023_SANTOS_IZAURA.pdf http://repositorio.ufsm.br/bitstream/1/29097/3/license.txt http://repositorio.ufsm.br/bitstream/1/29097/2/license_rdf |
bitstream.checksum.fl_str_mv |
2792f9d2d1ff3ec3d03470ceb1185a00 2f0571ecee68693bd5cd3f17c1e075df 4460e5956bc1d1639be9ae6146a50347 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
|
_version_ |
1794523802463371264 |