Extrato de semente de Syzygium cumini (L.) Skeels reduz o dano renal e hepático provocado pela exposição aguda ao metilmercúrio em ratos neonatos

Detalhes bibliográficos
Autor(a) principal: Abdalla, Faida Husein
Data de Publicação: 2010
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Manancial - Repositório Digital da UFSM
Texto Completo: http://repositorio.ufsm.br/handle/1/5918
Resumo: Methylmercury (MeHg) is a potent neuro and nephrotoxicant in several animal species including humans, particularly during their development. The purpose of this study was to investigate the effects of aqueous seed extract of Syzygium cumini (L.) Skeels (Scc) on the acute MeHg treatment in neonatal rats. Neonatal rats (P2) received orally a single dose of MeHg (10 mg/kg) and also two doses of Scc. After two days, the effects of this treatment were investigated in the cerebral cortex, hippocampus, kidney, liver and urine samples of rats. We observed that N-Acetyl-β-d-glucosaminidase (NAG) activity in the kidney and urine was higher in MeHg-group when compared with the control group. Similarly, the lipid peroxidation levels were higher in the liver and kidney as well as the Adenosine deaminase (ADA) activity increased in the hippocampus, kidney and liver. These results indicate that increased NAG and ADA activities, as well as thiobarbituric acid reactive species (TBARS) levels may play a critical role in MeHg nephrotoxicity. The most relevant finding in our investigation was that acute MeHg treatment in neonatal rats caused liver and renal impairment and Scc was able to prevent such effects. It appears that mechanisms related to scavenging activity of Scc could be involved with its protection effect. Key words: Methylmercury; Syzygium cumini; N-Acetyl-β-d-glucosaminidase; rat; adenosine deaminase
id UFSM-20_a3531430871e9246d6be790ed0d26d04
oai_identifier_str oai:repositorio.ufsm.br:1/5918
network_acronym_str UFSM-20
network_name_str Manancial - Repositório Digital da UFSM
repository_id_str 3913
spelling 2013-05-022013-05-022010-01-25ABDALLA, Faida Husein. SYZYGIUM CUMINI SEED EXTRACT PREVENT THE RENAL AND LIVER IMPAIRMENT CAUSED BY ACUTE METHYLMERCURY TREATMENT IN NEONATAL RATS. 2010. 80 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2010.http://repositorio.ufsm.br/handle/1/5918Methylmercury (MeHg) is a potent neuro and nephrotoxicant in several animal species including humans, particularly during their development. The purpose of this study was to investigate the effects of aqueous seed extract of Syzygium cumini (L.) Skeels (Scc) on the acute MeHg treatment in neonatal rats. Neonatal rats (P2) received orally a single dose of MeHg (10 mg/kg) and also two doses of Scc. After two days, the effects of this treatment were investigated in the cerebral cortex, hippocampus, kidney, liver and urine samples of rats. We observed that N-Acetyl-β-d-glucosaminidase (NAG) activity in the kidney and urine was higher in MeHg-group when compared with the control group. Similarly, the lipid peroxidation levels were higher in the liver and kidney as well as the Adenosine deaminase (ADA) activity increased in the hippocampus, kidney and liver. These results indicate that increased NAG and ADA activities, as well as thiobarbituric acid reactive species (TBARS) levels may play a critical role in MeHg nephrotoxicity. The most relevant finding in our investigation was that acute MeHg treatment in neonatal rats caused liver and renal impairment and Scc was able to prevent such effects. It appears that mechanisms related to scavenging activity of Scc could be involved with its protection effect. Key words: Methylmercury; Syzygium cumini; N-Acetyl-β-d-glucosaminidase; rat; adenosine deaminaseO metilmercúrio (MeHg) é um agente tóxico potente tanto para o sistema nervoso central como para o renal. Provoca danos nos seres humanos e em ratos, particularmente durante o estágio de desenvolvimento. Neste estudo, ratos em desenvolvimento (P2) receberam uma dose única de MeHg (10 mg/kg), por via oral e/ou duas doses do extrato aquoso de sementes de Syzygium cumini (L.) Skeels (Scc). Após dois dias (P4), foram investigados os efeitos deste tratamento no córtex cerebral, hipocampo, rim, fígado e urina. Observamos que a atividade da N-acetil-β-d-glicosaminidase (NAG) nos rins e na urina foi maior no grupo que recebeu MeHg, quando comparado com o grupo controle. Da mesma forma, os níveis de peroxidação lipídica foram maiores no fígado e rim e a atividade da adenosina deaminase (ADA) encontrou-se elevada no hipocampo, rins e fígado, nos ratos tratados com MeHg. Estes resultados indicam que o aumento da atividade da NAG e da ADA, bem como os níveis das espécies reativas ao ácido tiobarbitúrico (TBARS), desempenham um papel importante como marcadores de nefrotoxicidade causada pelo MeHg. Assim, o achado mais relevante na nossa investigação foi a de que o tratamento agudo com MeHg em ratos neonatos pode provocar nefrotoxicidade e a administração do Scc pode reverter estes efeitos provavelmente devido as propriedades antioxidantes de Scc.application/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Ciências FarmacêuticasUFSMBRAnálises Clínicas e ToxicológicasMetilmercúrioSyzygium cu-d-glucosaminidaseminiN-acetil-β-d-glucosaminidaseRatosAdenosina deaminaseMethylmercurySyzygium cuminiN-Acetyl-β-d-glucosaminidaseRatAdenosine deaminaseCNPQ::CIENCIAS DA SAUDE::FARMACIAExtrato de semente de Syzygium cumini (L.) Skeels reduz o dano renal e hepático provocado pela exposição aguda ao metilmercúrio em ratos neonatosSyzygium cumini seed extract prevent the renal and liver impairment caused by acute methylmercury treatment in neonatal ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisMoretto, Maria Beatrizhttp://lattes.cnpq.br/7317262818918502Posser, Thaishttp://lattes.cnpq.br/2277857386983441Franco, Jeferson Luishttp://lattes.cnpq.br/1680065573338339http://lattes.cnpq.br/8065173990084138Abdalla, Faida Husein20100000000040050030030050074ded2f6-b093-48aa-bcc3-f0f3d1669294a5cd131c-4834-4d53-abd8-56b2e0050d6aeed9356b-ea51-4cc4-9762-8e3a7b7e38f5423a65fa-1ad8-43d9-b50e-5041a255d6a0info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALABDALLA, FAIDA HUSEIN.pdfapplication/pdf1171916http://repositorio.ufsm.br/bitstream/1/5918/1/ABDALLA%2c%20FAIDA%20HUSEIN.pdfdef581eaaf65e58422bf123a75cb8df5MD51TEXTABDALLA, FAIDA HUSEIN.pdf.txtABDALLA, FAIDA HUSEIN.pdf.txtExtracted texttext/plain120445http://repositorio.ufsm.br/bitstream/1/5918/2/ABDALLA%2c%20FAIDA%20HUSEIN.pdf.txt4256da3a8cdc2bb6e11190b45510c2beMD52THUMBNAILABDALLA, FAIDA HUSEIN.pdf.jpgABDALLA, FAIDA HUSEIN.pdf.jpgIM Thumbnailimage/jpeg6406http://repositorio.ufsm.br/bitstream/1/5918/3/ABDALLA%2c%20FAIDA%20HUSEIN.pdf.jpg18bab590f5ff383cedc57df38a718470MD531/59182022-10-10 11:49:56.309oai:repositorio.ufsm.br:1/5918Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestopendoar:39132022-10-10T14:49:56Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.por.fl_str_mv Extrato de semente de Syzygium cumini (L.) Skeels reduz o dano renal e hepático provocado pela exposição aguda ao metilmercúrio em ratos neonatos
dc.title.alternative.eng.fl_str_mv Syzygium cumini seed extract prevent the renal and liver impairment caused by acute methylmercury treatment in neonatal rats
title Extrato de semente de Syzygium cumini (L.) Skeels reduz o dano renal e hepático provocado pela exposição aguda ao metilmercúrio em ratos neonatos
spellingShingle Extrato de semente de Syzygium cumini (L.) Skeels reduz o dano renal e hepático provocado pela exposição aguda ao metilmercúrio em ratos neonatos
Abdalla, Faida Husein
Metilmercúrio
Syzygium cu-d-glucosaminidasemini
N-acetil-β-d-glucosaminidase
Ratos
Adenosina deaminase
Methylmercury
Syzygium cumini
N-Acetyl-β-d-glucosaminidase
Rat
Adenosine deaminase
CNPQ::CIENCIAS DA SAUDE::FARMACIA
title_short Extrato de semente de Syzygium cumini (L.) Skeels reduz o dano renal e hepático provocado pela exposição aguda ao metilmercúrio em ratos neonatos
title_full Extrato de semente de Syzygium cumini (L.) Skeels reduz o dano renal e hepático provocado pela exposição aguda ao metilmercúrio em ratos neonatos
title_fullStr Extrato de semente de Syzygium cumini (L.) Skeels reduz o dano renal e hepático provocado pela exposição aguda ao metilmercúrio em ratos neonatos
title_full_unstemmed Extrato de semente de Syzygium cumini (L.) Skeels reduz o dano renal e hepático provocado pela exposição aguda ao metilmercúrio em ratos neonatos
title_sort Extrato de semente de Syzygium cumini (L.) Skeels reduz o dano renal e hepático provocado pela exposição aguda ao metilmercúrio em ratos neonatos
author Abdalla, Faida Husein
author_facet Abdalla, Faida Husein
author_role author
dc.contributor.advisor1.fl_str_mv Moretto, Maria Beatriz
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/7317262818918502
dc.contributor.referee1.fl_str_mv Posser, Thais
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/2277857386983441
dc.contributor.referee2.fl_str_mv Franco, Jeferson Luis
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/1680065573338339
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/8065173990084138
dc.contributor.author.fl_str_mv Abdalla, Faida Husein
contributor_str_mv Moretto, Maria Beatriz
Posser, Thais
Franco, Jeferson Luis
dc.subject.por.fl_str_mv Metilmercúrio
Syzygium cu-d-glucosaminidasemini
N-acetil-β-d-glucosaminidase
Ratos
Adenosina deaminase
topic Metilmercúrio
Syzygium cu-d-glucosaminidasemini
N-acetil-β-d-glucosaminidase
Ratos
Adenosina deaminase
Methylmercury
Syzygium cumini
N-Acetyl-β-d-glucosaminidase
Rat
Adenosine deaminase
CNPQ::CIENCIAS DA SAUDE::FARMACIA
dc.subject.eng.fl_str_mv Methylmercury
Syzygium cumini
N-Acetyl-β-d-glucosaminidase
Rat
Adenosine deaminase
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS DA SAUDE::FARMACIA
description Methylmercury (MeHg) is a potent neuro and nephrotoxicant in several animal species including humans, particularly during their development. The purpose of this study was to investigate the effects of aqueous seed extract of Syzygium cumini (L.) Skeels (Scc) on the acute MeHg treatment in neonatal rats. Neonatal rats (P2) received orally a single dose of MeHg (10 mg/kg) and also two doses of Scc. After two days, the effects of this treatment were investigated in the cerebral cortex, hippocampus, kidney, liver and urine samples of rats. We observed that N-Acetyl-β-d-glucosaminidase (NAG) activity in the kidney and urine was higher in MeHg-group when compared with the control group. Similarly, the lipid peroxidation levels were higher in the liver and kidney as well as the Adenosine deaminase (ADA) activity increased in the hippocampus, kidney and liver. These results indicate that increased NAG and ADA activities, as well as thiobarbituric acid reactive species (TBARS) levels may play a critical role in MeHg nephrotoxicity. The most relevant finding in our investigation was that acute MeHg treatment in neonatal rats caused liver and renal impairment and Scc was able to prevent such effects. It appears that mechanisms related to scavenging activity of Scc could be involved with its protection effect. Key words: Methylmercury; Syzygium cumini; N-Acetyl-β-d-glucosaminidase; rat; adenosine deaminase
publishDate 2010
dc.date.issued.fl_str_mv 2010-01-25
dc.date.accessioned.fl_str_mv 2013-05-02
dc.date.available.fl_str_mv 2013-05-02
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv ABDALLA, Faida Husein. SYZYGIUM CUMINI SEED EXTRACT PREVENT THE RENAL AND LIVER IMPAIRMENT CAUSED BY ACUTE METHYLMERCURY TREATMENT IN NEONATAL RATS. 2010. 80 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2010.
dc.identifier.uri.fl_str_mv http://repositorio.ufsm.br/handle/1/5918
identifier_str_mv ABDALLA, Faida Husein. SYZYGIUM CUMINI SEED EXTRACT PREVENT THE RENAL AND LIVER IMPAIRMENT CAUSED BY ACUTE METHYLMERCURY TREATMENT IN NEONATAL RATS. 2010. 80 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2010.
url http://repositorio.ufsm.br/handle/1/5918
dc.language.iso.fl_str_mv por
language por
dc.relation.cnpq.fl_str_mv 201000000000
dc.relation.confidence.fl_str_mv 400
500
300
300
500
dc.relation.authority.fl_str_mv 74ded2f6-b093-48aa-bcc3-f0f3d1669294
a5cd131c-4834-4d53-abd8-56b2e0050d6a
eed9356b-ea51-4cc4-9762-8e3a7b7e38f5
423a65fa-1ad8-43d9-b50e-5041a255d6a0
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Ciências Farmacêuticas
dc.publisher.initials.fl_str_mv UFSM
dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Análises Clínicas e Toxicológicas
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Manancial - Repositório Digital da UFSM
collection Manancial - Repositório Digital da UFSM
bitstream.url.fl_str_mv http://repositorio.ufsm.br/bitstream/1/5918/1/ABDALLA%2c%20FAIDA%20HUSEIN.pdf
http://repositorio.ufsm.br/bitstream/1/5918/2/ABDALLA%2c%20FAIDA%20HUSEIN.pdf.txt
http://repositorio.ufsm.br/bitstream/1/5918/3/ABDALLA%2c%20FAIDA%20HUSEIN.pdf.jpg
bitstream.checksum.fl_str_mv def581eaaf65e58422bf123a75cb8df5
4256da3a8cdc2bb6e11190b45510c2be
18bab590f5ff383cedc57df38a718470
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv
_version_ 1801223739503804416

Registros relacionados