Avaliação dos efeitos do citrato de sódio sobre o metabolismo lipídico e da acetilcolina em camundongos
Autor(a) principal: | |
---|---|
Data de Publicação: | 2002 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/26665 |
Resumo: | The sodium citrate is one of the most utilized in chemical food additives that have been found in tinned and milk derivatives. Its proprieties are: stabilizer, emulsifier and acidity regulator. The chemical compound can be dissociated in water by releasing its anion. The endogenous citrate in biological systems plays an important role in the general metabolism, serving as a source of acetyl-CoA that can be utilized for the synthesis of lipids and acetylcholine. The citrate also has an important role in controlling the effects on enzymes as Phosphofructokinase and Acetyl-CoA carboxylase. The aims of this study are the investigations on the effects of the additive over lipid and acetylcholine metabolism together with the evaluation of biochemical parameters with ions Na+, K+, urea and creatinine. The mice were separated in two different treatments: subchronic (55 days) and chronic (110 days), males and females. The sodium citrate solutions were control (potable water), 1%, 2.5% and 5% ad libitum in drinking water. The results showed an increase of the body weight in 2.5% concentration for both subchronic and chronic treatments. In the inner organs, the study showed a decrease of livers weight in 5% concentration. In the histological analysis we observed that the animal cells in 2.5% concentration had more capacity of lipid saving, but in 5% this capacity is decreased. It was observed an increase on the quantify of muscle lipids on the male in both treatments. The lipase evaluation has shown a decrease on the subchronic males in 2.5% and 5% sodium citrate concentrations. Didn’t appear changes in K+ levels. In the ACh metabolism had an increase of AChE in the subchronic male and chronic female in vivo treatments. The in vitro experiments had a tendency for activity increasing AChE in sodium citrate 50 mM. The objective of this work was to recover aspects around the application of sodium citrate and its possible interactions in lipid metabolism and acetylcholinesterase activity. |
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2022-10-25T17:28:49Z2022-10-25T17:28:49Z2002-07-30http://repositorio.ufsm.br/handle/1/26665The sodium citrate is one of the most utilized in chemical food additives that have been found in tinned and milk derivatives. Its proprieties are: stabilizer, emulsifier and acidity regulator. The chemical compound can be dissociated in water by releasing its anion. The endogenous citrate in biological systems plays an important role in the general metabolism, serving as a source of acetyl-CoA that can be utilized for the synthesis of lipids and acetylcholine. The citrate also has an important role in controlling the effects on enzymes as Phosphofructokinase and Acetyl-CoA carboxylase. The aims of this study are the investigations on the effects of the additive over lipid and acetylcholine metabolism together with the evaluation of biochemical parameters with ions Na+, K+, urea and creatinine. The mice were separated in two different treatments: subchronic (55 days) and chronic (110 days), males and females. The sodium citrate solutions were control (potable water), 1%, 2.5% and 5% ad libitum in drinking water. The results showed an increase of the body weight in 2.5% concentration for both subchronic and chronic treatments. In the inner organs, the study showed a decrease of livers weight in 5% concentration. In the histological analysis we observed that the animal cells in 2.5% concentration had more capacity of lipid saving, but in 5% this capacity is decreased. It was observed an increase on the quantify of muscle lipids on the male in both treatments. The lipase evaluation has shown a decrease on the subchronic males in 2.5% and 5% sodium citrate concentrations. Didn’t appear changes in K+ levels. In the ACh metabolism had an increase of AChE in the subchronic male and chronic female in vivo treatments. The in vitro experiments had a tendency for activity increasing AChE in sodium citrate 50 mM. The objective of this work was to recover aspects around the application of sodium citrate and its possible interactions in lipid metabolism and acetylcholinesterase activity.O citrato de sódio é um dos aditivos químicos mais utilizados pela indústria alimentícia sendo encontrado em embutidos e derivados do leite. Suas propriedades são de estabilizante, emulsificante e regulador de acidez. O composto químico pode ser dissociado em meio aquoso liberando seu ânion. Já o citrato endógeno nos sistemas biológicos exerce um importante papel no metabolismo em geral, pois tem a capacidade de suprir o conteúdo citoplasmático de unidades de acetil-CoA que podem ser utilizadas para a produção de lipídios e da acetilcolina. O citrato também exerce efeitos importantes modulando enzimas como a fosfofrutoquinase e acetil-CoA-carboxilase. No presente estudo investigou-se o efeito do aditivo sobre o metabolismo lipídico e da acetilcolina juntamente com a avaliação de parâmetros bioquímicos como os íons Na+, K+, uréia e creatinina. Os camundongos foram divididos em tratamentos subcrônicos (55 dias) e crônicos (110 dias), machos e fêmeas. As soluções de citrato de sódio foram controle (água potável), 1%, 2,5% e 5% ad libitum na água de beber. Os resultados demonstraram um aumento dos pesos corporais na concentração 2,5%, e uma diminuição dos pesos em 5% em ambos os tratamentos subcrônico como crônico. Na pesagem dos órgãos internos, evidenciamos a diminuição dos pesos dos fígados em 5%. Frente à análise histológica observamos que as células dos animais 2,5% possuem maior capacidade de armazenamento lipídico, já em 5% a capacidade é diminuída. Na quantificação dos lipídios musculares, foi observado uma maior quantidade de lipídios nos machos de ambos os tratamentos 2,5%. A avaliação da lipase plasmática, demonstrou uma diminuição nos machos subcrônicos 2.5% e 5%. Também, houve uma diminuição dos triglicerídeos plasmáticos dos grupos em 5%. As análises bioquímicas demonstram um aumento no Na+ plasmático em 5% bem como um aumento na uréia e na creatinina nos grupos citrato de sódio 5%. Não houveram alterações na concentração plasmática do íon K+. Frente ao metabolismo da ACh percebe-se um aumento da AChE nos tratamentos machos subcrônicos e fêmeas crônicas in vivo. No experimento in vitro notamos uma tendência ao aumento da AChE na concentração final 50 mM de citrato de sódio. O trabalho realizado teve o intuito de reavaliar aspectos sobre a aplicabilidade do citrato de sódio e suas possíveis interações no metabolismo lipídico e na atividade da acetilcolinesterase.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBrasilCiências BiológicasAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessMetabolismo lipídicoCitrato de sódioAditivos químicosBioquímicaConservantesEstabilizantesEnzimasAcetilcolinaToxicologiaCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAAvaliação dos efeitos do citrato de sódio sobre o metabolismo lipídico e da acetilcolina em camundongosEvaluation of effects the sodium citrate about lipid and acetylcholine metabolism in miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisChitolina, Maria Rosahttp://lattes.cnpq.br/4401319386725357Wyse, Angela Terezinha de SouzaSilveira, Aron Ferreira dahttp://lattes.cnpq.br/4244248791455177Balz, Daniela2008000000026006006006006003a9dc4db-b442-4eab-840e-5158c5a64c7c7dcef848-920f-4f4b-98c8-4aa2bd4ab46ce75072e6-bd77-4a58-bc22-0e14dad779a3d25c454c-3e5a-49ff-8801-28f584d78192reponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDIS_PPGCBBT_2002_BALZ_DANIELA.pdfDIS_PPGCBBT_2002_BALZ_DANIELA.pdfDissertação de Mestradoapplication/pdf989941http://repositorio.ufsm.br/bitstream/1/26665/1/DIS_PPGCBBT_2002_BALZ_DANIELA.pdf6d7645fa115b100acf09c4b359efa745MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/26665/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81956http://repositorio.ufsm.br/bitstream/1/26665/3/license.txt2f0571ecee68693bd5cd3f17c1e075dfMD531/266652022-10-25 14:28:49.483oai:repositorio.ufsm.br:1/26665TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkNvbSBhIGFwcmVzZW50YcOnw6NvIGRlc3RhIGxpY2Vuw6dhLCB2b2PDqiAobyBhdXRvciAoZXMpIG91IG8gdGl0dWxhciBkb3MgZGlyZWl0b3MgZGUgYXV0b3IpIGNvbmNlZGUgw6AgVW5pdmVyc2lkYWRlCkZlZGVyYWwgZGUgU2FudGEgTWFyaWEgKFVGU00pIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSByZXByb2R1emlyLCAgdHJhZHV6aXIgKGNvbmZvcm1lIGRlZmluaWRvIGFiYWl4byksIGUvb3UKZGlzdHJpYnVpciBhIHN1YSB0ZXNlIG91IGRpc3NlcnRhw6fDo28gKGluY2x1aW5kbyBvIHJlc3VtbykgcG9yIHRvZG8gbyBtdW5kbyBubyBmb3JtYXRvIGltcHJlc3NvIGUgZWxldHLDtG5pY28gZQplbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mgw6F1ZGlvIG91IHbDrWRlby4KClZvY8OqIGNvbmNvcmRhIHF1ZSBhIFVGU00gcG9kZSwgc2VtIGFsdGVyYXIgbyBjb250ZcO6ZG8sIHRyYW5zcG9yIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbwpwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBwYXJhIGZpbnMgZGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIHRhbWLDqW0gY29uY29yZGEgcXVlIGEgVUZTTSBwb2RlIG1hbnRlciBtYWlzIGRlIHVtYSBjw7NwaWEgYSBzdWEgdGVzZSBvdQpkaXNzZXJ0YcOnw6NvIHBhcmEgZmlucyBkZSBzZWd1cmFuw6dhLCBiYWNrLXVwIGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIGRlY2xhcmEgcXVlIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyDDqSBvcmlnaW5hbCBlIHF1ZSB2b2PDqiB0ZW0gbyBwb2RlciBkZSBjb25jZWRlciBvcyBkaXJlaXRvcyBjb250aWRvcwpuZXN0YSBsaWNlbsOnYS4gVm9jw6ogdGFtYsOpbSBkZWNsYXJhIHF1ZSBvIGRlcMOzc2l0byBkYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIG7Do28sIHF1ZSBzZWphIGRlIHNldQpjb25oZWNpbWVudG8sIGluZnJpbmdlIGRpcmVpdG9zIGF1dG9yYWlzIGRlIG5pbmd1w6ltLgoKQ2FzbyBhIHN1YSB0ZXNlIG91IGRpc3NlcnRhw6fDo28gY29udGVuaGEgbWF0ZXJpYWwgcXVlIHZvY8OqIG7Do28gcG9zc3VpIGEgdGl0dWxhcmlkYWRlIGRvcyBkaXJlaXRvcyBhdXRvcmFpcywgdm9jw6oKZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzc8OjbyBpcnJlc3RyaXRhIGRvIGRldGVudG9yIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBwYXJhIGNvbmNlZGVyIMOgIFVGU00Kb3MgZGlyZWl0b3MgYXByZXNlbnRhZG9zIG5lc3RhIGxpY2Vuw6dhLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlCmlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIG5vIHRleHRvIG91IG5vIGNvbnRlw7pkbyBkYSB0ZXNlIG91IGRpc3NlcnRhw6fDo28gb3JhIGRlcG9zaXRhZGEuCgpDQVNPIEEgVEVTRSBPVSBESVNTRVJUQcOHw4NPIE9SQSBERVBPU0lUQURBIFRFTkhBIFNJRE8gUkVTVUxUQURPIERFIFVNIFBBVFJPQ8ONTklPIE9VCkFQT0lPIERFIFVNQSBBR8OKTkNJQSBERSBGT01FTlRPIE9VIE9VVFJPIE9SR0FOSVNNTyBRVUUgTsODTyBTRUpBIEEgVUZTTQosIFZPQ8OKIERFQ0xBUkEgUVVFIFJFU1BFSVRPVSBUT0RPUyBFIFFVQUlTUVVFUiBESVJFSVRPUyBERSBSRVZJU8ODTyBDT01PClRBTULDiU0gQVMgREVNQUlTIE9CUklHQcOHw5VFUyBFWElHSURBUyBQT1IgQ09OVFJBVE8gT1UgQUNPUkRPLgoKQSBVRlNNIHNlIGNvbXByb21ldGUgYSBpZGVudGlmaWNhciBjbGFyYW1lbnRlIG8gc2V1IG5vbWUgKHMpIG91IG8ocykgbm9tZShzKSBkbyhzKQpkZXRlbnRvcihlcykgZG9zIGRpcmVpdG9zIGF1dG9yYWlzIGRhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbywgZSBuw6NvIGZhcsOhIHF1YWxxdWVyIGFsdGVyYcOnw6NvLCBhbMOpbSBkYXF1ZWxhcwpjb25jZWRpZGFzIHBvciBlc3RhIGxpY2Vuw6dhLgoKRepositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestopendoar:39132022-10-25T17:28:49Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Avaliação dos efeitos do citrato de sódio sobre o metabolismo lipídico e da acetilcolina em camundongos |
dc.title.alternative.eng.fl_str_mv |
Evaluation of effects the sodium citrate about lipid and acetylcholine metabolism in mice |
title |
Avaliação dos efeitos do citrato de sódio sobre o metabolismo lipídico e da acetilcolina em camundongos |
spellingShingle |
Avaliação dos efeitos do citrato de sódio sobre o metabolismo lipídico e da acetilcolina em camundongos Balz, Daniela Metabolismo lipídico Citrato de sódio Aditivos químicos Bioquímica Conservantes Estabilizantes Enzimas Acetilcolina Toxicologia CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Avaliação dos efeitos do citrato de sódio sobre o metabolismo lipídico e da acetilcolina em camundongos |
title_full |
Avaliação dos efeitos do citrato de sódio sobre o metabolismo lipídico e da acetilcolina em camundongos |
title_fullStr |
Avaliação dos efeitos do citrato de sódio sobre o metabolismo lipídico e da acetilcolina em camundongos |
title_full_unstemmed |
Avaliação dos efeitos do citrato de sódio sobre o metabolismo lipídico e da acetilcolina em camundongos |
title_sort |
Avaliação dos efeitos do citrato de sódio sobre o metabolismo lipídico e da acetilcolina em camundongos |
author |
Balz, Daniela |
author_facet |
Balz, Daniela |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Chitolina, Maria Rosa |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/4401319386725357 |
dc.contributor.referee1.fl_str_mv |
Wyse, Angela Terezinha de Souza |
dc.contributor.referee2.fl_str_mv |
Silveira, Aron Ferreira da |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/4244248791455177 |
dc.contributor.author.fl_str_mv |
Balz, Daniela |
contributor_str_mv |
Chitolina, Maria Rosa Wyse, Angela Terezinha de Souza Silveira, Aron Ferreira da |
dc.subject.por.fl_str_mv |
Metabolismo lipídico Citrato de sódio Aditivos químicos Bioquímica Conservantes Estabilizantes Enzimas Acetilcolina Toxicologia |
topic |
Metabolismo lipídico Citrato de sódio Aditivos químicos Bioquímica Conservantes Estabilizantes Enzimas Acetilcolina Toxicologia CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
The sodium citrate is one of the most utilized in chemical food additives that have been found in tinned and milk derivatives. Its proprieties are: stabilizer, emulsifier and acidity regulator. The chemical compound can be dissociated in water by releasing its anion. The endogenous citrate in biological systems plays an important role in the general metabolism, serving as a source of acetyl-CoA that can be utilized for the synthesis of lipids and acetylcholine. The citrate also has an important role in controlling the effects on enzymes as Phosphofructokinase and Acetyl-CoA carboxylase. The aims of this study are the investigations on the effects of the additive over lipid and acetylcholine metabolism together with the evaluation of biochemical parameters with ions Na+, K+, urea and creatinine. The mice were separated in two different treatments: subchronic (55 days) and chronic (110 days), males and females. The sodium citrate solutions were control (potable water), 1%, 2.5% and 5% ad libitum in drinking water. The results showed an increase of the body weight in 2.5% concentration for both subchronic and chronic treatments. In the inner organs, the study showed a decrease of livers weight in 5% concentration. In the histological analysis we observed that the animal cells in 2.5% concentration had more capacity of lipid saving, but in 5% this capacity is decreased. It was observed an increase on the quantify of muscle lipids on the male in both treatments. The lipase evaluation has shown a decrease on the subchronic males in 2.5% and 5% sodium citrate concentrations. Didn’t appear changes in K+ levels. In the ACh metabolism had an increase of AChE in the subchronic male and chronic female in vivo treatments. The in vitro experiments had a tendency for activity increasing AChE in sodium citrate 50 mM. The objective of this work was to recover aspects around the application of sodium citrate and its possible interactions in lipid metabolism and acetylcholinesterase activity. |
publishDate |
2002 |
dc.date.issued.fl_str_mv |
2002-07-30 |
dc.date.accessioned.fl_str_mv |
2022-10-25T17:28:49Z |
dc.date.available.fl_str_mv |
2022-10-25T17:28:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/26665 |
url |
http://repositorio.ufsm.br/handle/1/26665 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
200800000002 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 600 |
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dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Ciências Biológicas |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
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