Análise genotípica e filogenética com base nos genes das glicoproteínas C e D de herpesvírus bovino 1 e 5

• Autor(a) principal: Traesel, Carolina Kist
• Data de Publicação: 2013
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Manancial - Repositório Digital da UFSM
• Texto Completo: http://repositorio.ufsm.br/handle/1/4095
• topic: BoHV-1; BoHV-5; GC; GD; Filogenia; Phylogeny; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA

Bovine herpesviruses 1 (BoHV-1) and 5 (BoHV-5) are genetic and antigenic closely related pathogens of cattle, historically associated with respiratory/genital and neurological disease, respectively. This thesis reports the genomic and phylogenetic analyzes based on glycoproteins C (gC) and D (gD) genes of BoHV-1 and BoHV-5. Initially, the differentiation between BoHV-1 and BoHV-5 was performed by a differential PCR based on 5 gC gene. Then, a molecular analysis based on the 3 region of the gC gene of 45 BoHV isolates from Brazil, Uruguay and Argentina (1981-2009) was described. The phylogenetic tree reconstruction provided a clear distinction between BoHV-1 and BoHV-5, and BoHV-1 into subtypes BoHV-1.1 and BoHV-1.2. The levels of nucleotide (nt) similarity ranged from 99.1 to 100% among BoHV-1 sequences (n=12); 96.2-100% among BoHV-5 sequences (n=32); and 77.7-90.3% between BoHV-1 and BoHV-5 sequences. A transmembrane domain of 24 amino-acid (aa) and the putative cytoplasmic tail of 8 aa were also identified. In addition, a phylogenetic study was performed to investigate genetic divergences at the 3 region of gD gene of respiratory/genital BoHV-1 (n=7), neurological BoHV-1 (n=7) and neurological BoHV-5 (n=7) isolates, and whether these differences would be associated with the respective neurological presentation. The phylogenetic reconstruction allowed a clear differentiation of BoHV-1 (n=14) and BoHV-5 (n=7), but BoHV-1 isolates from neurological disease grouped within BoHV-1 branch. The nt and aa similarity levels were on average 98.3% among BoHV-1; 97.8% and 95.8% among BoHV-5; 73.7% and 64.1% between viral species. These results indicate that both genes revealed a high conserved 3 region within each species and a less conserved region between BoHV-1 and BoHV-5. The phylogenetic analyzes allowed differentiation of BoHV-1 and BoHV-5 species, and even subtypes grouped in distinct branches in the 3 gC gene-based study, indicating that this region represents a better choice for phylogenetic subgrouping. So, it was concluded that these genome regions represent a suitable target for phylogenetic classification of BoHV-1 and BoHV-5 isolates, and, perhaps, for understanding evolutionary relationships. However, no conclusion of a possible association of genetic differences with phenotypes could be drawn.