Propriedades de proteção gastrointestinal da Rosmarinus officinalis l. em associação a testes microbiológicos e antioxidantes in vitro e ex vivo
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/18218 |
Resumo: | The elevate production of reactive species over physiological levels and associated to pathogenic bacteria could represent a high risk for many diseases. The high levels of oxidative stress and inflammation can be present in the etiology of gastrointestinal pathologies associated with ethanol ingestion, and sometimes with implication in liver or brain. Peptic ulcer includes gastric and intestinal damage that affect many people around the world and its development is a result of the imbalance between aggressive and protective factors. The Rosmarinus officinalis L., more commonly known as rosemary in Europe and as alecrim in Brazil, has exhibited several physiological and medicinal activities against some diseases mainly due its phenolic compounds. So in this thesis, the aim was to perform analyzes in vitro and ex vivo on the antioxidant properties of the ethanolic extract of Rosmarinus officinalis L. (eeRo) and its fractions (DCM, EA, ButOH), and about their antibacterial activities and the possible applicability of eeRo on gastrointestinal protection. The eeRo was obtained from the dried leaves (40ºC) subjected to an alcoholic extraction in the soxhlet apparatus. The eeRo was separately to dichloromethane, ethyl acetate and butanol in a separator funnel to get DCM, EA and ButOH fractions, respectively. The quantification of constituents of eeRo and its fractions were made by HPLC–DAD. The eeRo and its fractions were tested in the DPPH•- radical scavenging, total antioxidant capacity assays without tissues and they were tested in sodium nitroprusside-induced lipid peroxidation and H2DCF-DA in liver, brain and stomach of male adult wistar rats. The eeRo and its fractions were analyzed in bacteria minimum inhibitory concentration assay. Besids, eeRo and its fractions were tested in ethanol-induced gastric and intestinal lesions models. After application of these models and pretreatment with eeRo the dichlorofluorescein fluorescence, lipid peroxidation, ratio of GSH/GSSG, superoxide dismutase activity, catalase activity, myeloperoxidase activity assays were performed in stomach and intestine. Moreover, the measurement of nitrite and nitrate levels, ulcer index and histopathology tests were made only in stomach. The Na+/K+ ATPase activity was measured only in intestine. The eeRo, DCM, EA had significant total antioxidant effect and DPPH•- radical scavenging activity in vitro. The DCM and eeRo got antioxidant effects against basal levels of reactive species in the liver, stomach and brain. The eeRo and DCM protected the liver and brain against lipid peroxidation induced by sodium nitroprussiate. The eeRo, DCM, EA and ButOH had inhibitory effect in the gram (+) and gram (-) bacteria. However, the eeRo and DCM, had the lower minimum inhibitory concentration.The eeRo, after in vivo treatment protected stomach and intestine against the lipid peroxidation and they increased the CAT activity. The eeRo prevented the reduction in Na+/K+ ATPase and cased the increase of superoxide dismutase activity in intestine. In addition, eeRo reduced the myeloperoxidase activity in stomach and intestine. The results of this tese suggest that the eeRo, in general way, represented the better option as agent against oxidative stress in vitro, ex vivo e mainly in the prevention of gastrointestinal lesions in association to anti-inflamatory or vasodilatory mechanisms. |
id |
UFSM-20_d4598b3ae22a1fd073209ade46b370fa |
---|---|
oai_identifier_str |
oai:repositorio.ufsm.br:1/18218 |
network_acronym_str |
UFSM-20 |
network_name_str |
Manancial - Repositório Digital da UFSM |
repository_id_str |
3913 |
spelling |
2019-09-12T18:18:02Z2019-09-12T18:18:02Z2016-03-18http://repositorio.ufsm.br/handle/1/18218The elevate production of reactive species over physiological levels and associated to pathogenic bacteria could represent a high risk for many diseases. The high levels of oxidative stress and inflammation can be present in the etiology of gastrointestinal pathologies associated with ethanol ingestion, and sometimes with implication in liver or brain. Peptic ulcer includes gastric and intestinal damage that affect many people around the world and its development is a result of the imbalance between aggressive and protective factors. The Rosmarinus officinalis L., more commonly known as rosemary in Europe and as alecrim in Brazil, has exhibited several physiological and medicinal activities against some diseases mainly due its phenolic compounds. So in this thesis, the aim was to perform analyzes in vitro and ex vivo on the antioxidant properties of the ethanolic extract of Rosmarinus officinalis L. (eeRo) and its fractions (DCM, EA, ButOH), and about their antibacterial activities and the possible applicability of eeRo on gastrointestinal protection. The eeRo was obtained from the dried leaves (40ºC) subjected to an alcoholic extraction in the soxhlet apparatus. The eeRo was separately to dichloromethane, ethyl acetate and butanol in a separator funnel to get DCM, EA and ButOH fractions, respectively. The quantification of constituents of eeRo and its fractions were made by HPLC–DAD. The eeRo and its fractions were tested in the DPPH•- radical scavenging, total antioxidant capacity assays without tissues and they were tested in sodium nitroprusside-induced lipid peroxidation and H2DCF-DA in liver, brain and stomach of male adult wistar rats. The eeRo and its fractions were analyzed in bacteria minimum inhibitory concentration assay. Besids, eeRo and its fractions were tested in ethanol-induced gastric and intestinal lesions models. After application of these models and pretreatment with eeRo the dichlorofluorescein fluorescence, lipid peroxidation, ratio of GSH/GSSG, superoxide dismutase activity, catalase activity, myeloperoxidase activity assays were performed in stomach and intestine. Moreover, the measurement of nitrite and nitrate levels, ulcer index and histopathology tests were made only in stomach. The Na+/K+ ATPase activity was measured only in intestine. The eeRo, DCM, EA had significant total antioxidant effect and DPPH•- radical scavenging activity in vitro. The DCM and eeRo got antioxidant effects against basal levels of reactive species in the liver, stomach and brain. The eeRo and DCM protected the liver and brain against lipid peroxidation induced by sodium nitroprussiate. The eeRo, DCM, EA and ButOH had inhibitory effect in the gram (+) and gram (-) bacteria. However, the eeRo and DCM, had the lower minimum inhibitory concentration.The eeRo, after in vivo treatment protected stomach and intestine against the lipid peroxidation and they increased the CAT activity. The eeRo prevented the reduction in Na+/K+ ATPase and cased the increase of superoxide dismutase activity in intestine. In addition, eeRo reduced the myeloperoxidase activity in stomach and intestine. The results of this tese suggest that the eeRo, in general way, represented the better option as agent against oxidative stress in vitro, ex vivo e mainly in the prevention of gastrointestinal lesions in association to anti-inflamatory or vasodilatory mechanisms.A produção de espécies reativas acima dos níveis fisiológicos representa um alto risco para o surgimento de muitas patologias. Neste contexto, o estresse oxidativo e a inflamação podem estar presentes na etiologia de patologias gastrointestinais, como a úlcera péptica, associadas a ingestão de etanol, e por vezes, apresentam implicações hepáticas e neurais. A úlcera péptica é composta por danos gastrointestinais os quais afetam muitas pessoas em todo o mundo e seu desenvolvimento é resultado do desequilíbrio entre os fatores de proteção e agressão gástricos. A Rosmarinus officinalis L. é popularmente conhecida pelas suas propriedades medicinais em várias patologias, principalmente devido a presença de compostos fenólicos em sua composição. Assim, nesta tese o objetivo foi realizar análises in vitro e ex vivo sobre as propriedades antioxidantes do extrato etanólico de Rosmarinus officinalis L. (eeRo) e suas frações (DCM, EA, ButOH), além de investigar as suas atividades antibacterianas e a possível aplicabilidade do eeRo na proteção gastrointestinal. O eeRo foi obtido a partir de folhas secas (40ºC) e submetidas a uma extração alcóolica em aparelho de soxhlet. O eeRo foi separado por diclorometano, acetato de etila a butanol em um funil de separação para obter as frações DCM, EA e ButOH, respectivamente. A quantificação dos constituintes do eeRo e suas frações foi realizada por HPLC–DAD. O eeRo e suas frações foram testados como “scaveging” de radical DPPH•- e sobre capacidade antioxidante total sem a utilização de tecido e em ensaios de peroxidação lipídica induzida por nitroprussiato de sódio e fluorescência de diclorofluorsceína em fígado, cérebro e estômago de ratos adultos machos. O eeRo e suas frações foram testados no ensaio de concentração inibitória mínima em bactérias. Além disso, o eeRo e suas frações foram analizados em modelos de lesão gástrica e intestinal. Após a aplicação desses modelos e o prévio tratamento com eeRo foram realizados os ensaios de fluorescência de dilorofluorceína diacetato, peroxidação lipídica, taxa de GSH/GSSG, atividade da superóxido dismutase, catalase e mieloperoxidase em estômago e intestino de ratos. Além disso, foram mensurados os níveis de nitrito e nitrato, índice de ulceração além da realização de testes histopatológicas apenas em estômago. A atividade da Na+/K+-ATPase foi mensurada apenas em intestino. Os eeRo, DCM, EA tiveram, in vitro, efeito antioxidante total e “scavenging” de radical DPPH•-. O eeRo e a DCM apresentaram efeito antioxidante contra espécies reativas de oxigênio em fígado, estômago e cérebro. O eeRO e a DCM protegeram amostras de fígado e cérebro contra a peroxidação lipídica induzida por nitroprussiato de sódio. O eeRo e as DCM, EA and ButOH tiveram efeitos inibitórios sobre bactérias gram (+) e gram (- ). Contudo, o eeRo e a DCM tiveram as menores concentrações inibitórias. O eeRo, após um tratamento in vivo, protegeu o estômago e o intestino contra a peroxidação lipídica e aumentou a atividade da catalase em ambos estômago e intestino. O eeRo evitou a alteração nos níveis basais da Na+/K+-ATPase e aumentou a atividade da superóxido dismutase apenas no intestino. Além disso, o eeRo reduziu a atividade da mieloperoxidase em estômago e intestino. Os resultados desta tese sugerem que o eeRo, de modo geral, representa uma importante opção contra o estresse oxidativo in vitro, ex vivo e na prevenção de lesões gastrointestinais com mecanismos associados a ação anti-inflamatória ou vasodilatora.Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqporUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBrasilBioquímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAntioxidanteAntibacterianoProteção gastrointestinalÚlceraRosmarinus officinalis L.Anti-bacterialGastrointestinal protectionUlcerCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAPropriedades de proteção gastrointestinal da Rosmarinus officinalis l. em associação a testes microbiológicos e antioxidantes in vitro e ex vivoGastrointestinal protection properties of Rosmarinus officinalis l. in association with in vitro and ex vivo antioxidant and microbiological testsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisFachinetto, Roseleihttp://lattes.cnpq.br/7203076675431306Menezes, Irwin Rose Alencar dehttp://lattes.cnpq.br/6310868104861653Pereira, Maria Esterhttp://lattes.cnpq.br/9299114496157799Wagner, Carolinehttp://lattes.cnpq.br/4004565241849091Bauermann, Liliane de Freitashttp://lattes.cnpq.br/5849925846135968http://lattes.cnpq.br/6290560368071164Amaral, Guilherme Pires200800000002600e99bf03a-61e7-46c9-97d1-a7255476d47fbd098fe4-13d5-41ad-893e-351b974930e7658a0796-2a30-4f4c-84ca-d7562143b855276bcfc2-822c-4d05-88f0-be6c067a20c1d7dc21db-e508-4e38-b2e9-c73d2ec659272692a8bc-fe74-4278-b83e-83c3d8a4024dreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/18218/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD52ORIGINALTES_PPGBT_2016_AMARAL_GUILHERME.pdfTES_PPGBT_2016_AMARAL_GUILHERME.pdfTese de Doutoradoapplication/pdf6128116http://repositorio.ufsm.br/bitstream/1/18218/1/TES_PPGBT_2016_AMARAL_GUILHERME.pdf7569d61a27eb130a53e75b4943188075MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81956http://repositorio.ufsm.br/bitstream/1/18218/3/license.txt2f0571ecee68693bd5cd3f17c1e075dfMD53TEXTTES_PPGBT_2016_AMARAL_GUILHERME.pdf.txtTES_PPGBT_2016_AMARAL_GUILHERME.pdf.txtExtracted texttext/plain177995http://repositorio.ufsm.br/bitstream/1/18218/4/TES_PPGBT_2016_AMARAL_GUILHERME.pdf.txt2c462a6ea051f00955d1f8fa75be62cfMD54THUMBNAILTES_PPGBT_2016_AMARAL_GUILHERME.pdf.jpgTES_PPGBT_2016_AMARAL_GUILHERME.pdf.jpgIM Thumbnailimage/jpeg4690http://repositorio.ufsm.br/bitstream/1/18218/5/TES_PPGBT_2016_AMARAL_GUILHERME.pdf.jpgb37a8c0d5ca841e9cd936cd71a6a553bMD551/182182019-09-13 03:02:17.082oai:repositorio.ufsm.br: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ório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestopendoar:39132019-09-13T06:02:17Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Propriedades de proteção gastrointestinal da Rosmarinus officinalis l. em associação a testes microbiológicos e antioxidantes in vitro e ex vivo |
dc.title.alternative.eng.fl_str_mv |
Gastrointestinal protection properties of Rosmarinus officinalis l. in association with in vitro and ex vivo antioxidant and microbiological tests |
title |
Propriedades de proteção gastrointestinal da Rosmarinus officinalis l. em associação a testes microbiológicos e antioxidantes in vitro e ex vivo |
spellingShingle |
Propriedades de proteção gastrointestinal da Rosmarinus officinalis l. em associação a testes microbiológicos e antioxidantes in vitro e ex vivo Amaral, Guilherme Pires Antioxidante Antibacteriano Proteção gastrointestinal Úlcera Rosmarinus officinalis L. Anti-bacterial Gastrointestinal protection Ulcer CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Propriedades de proteção gastrointestinal da Rosmarinus officinalis l. em associação a testes microbiológicos e antioxidantes in vitro e ex vivo |
title_full |
Propriedades de proteção gastrointestinal da Rosmarinus officinalis l. em associação a testes microbiológicos e antioxidantes in vitro e ex vivo |
title_fullStr |
Propriedades de proteção gastrointestinal da Rosmarinus officinalis l. em associação a testes microbiológicos e antioxidantes in vitro e ex vivo |
title_full_unstemmed |
Propriedades de proteção gastrointestinal da Rosmarinus officinalis l. em associação a testes microbiológicos e antioxidantes in vitro e ex vivo |
title_sort |
Propriedades de proteção gastrointestinal da Rosmarinus officinalis l. em associação a testes microbiológicos e antioxidantes in vitro e ex vivo |
author |
Amaral, Guilherme Pires |
author_facet |
Amaral, Guilherme Pires |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Fachinetto, Roselei |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/7203076675431306 |
dc.contributor.referee1.fl_str_mv |
Menezes, Irwin Rose Alencar de |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/6310868104861653 |
dc.contributor.referee2.fl_str_mv |
Pereira, Maria Ester |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/9299114496157799 |
dc.contributor.referee3.fl_str_mv |
Wagner, Caroline |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/4004565241849091 |
dc.contributor.referee4.fl_str_mv |
Bauermann, Liliane de Freitas |
dc.contributor.referee4Lattes.fl_str_mv |
http://lattes.cnpq.br/5849925846135968 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/6290560368071164 |
dc.contributor.author.fl_str_mv |
Amaral, Guilherme Pires |
contributor_str_mv |
Fachinetto, Roselei Menezes, Irwin Rose Alencar de Pereira, Maria Ester Wagner, Caroline Bauermann, Liliane de Freitas |
dc.subject.por.fl_str_mv |
Antioxidante Antibacteriano Proteção gastrointestinal Úlcera Rosmarinus officinalis L. |
topic |
Antioxidante Antibacteriano Proteção gastrointestinal Úlcera Rosmarinus officinalis L. Anti-bacterial Gastrointestinal protection Ulcer CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Anti-bacterial Gastrointestinal protection Ulcer |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
The elevate production of reactive species over physiological levels and associated to pathogenic bacteria could represent a high risk for many diseases. The high levels of oxidative stress and inflammation can be present in the etiology of gastrointestinal pathologies associated with ethanol ingestion, and sometimes with implication in liver or brain. Peptic ulcer includes gastric and intestinal damage that affect many people around the world and its development is a result of the imbalance between aggressive and protective factors. The Rosmarinus officinalis L., more commonly known as rosemary in Europe and as alecrim in Brazil, has exhibited several physiological and medicinal activities against some diseases mainly due its phenolic compounds. So in this thesis, the aim was to perform analyzes in vitro and ex vivo on the antioxidant properties of the ethanolic extract of Rosmarinus officinalis L. (eeRo) and its fractions (DCM, EA, ButOH), and about their antibacterial activities and the possible applicability of eeRo on gastrointestinal protection. The eeRo was obtained from the dried leaves (40ºC) subjected to an alcoholic extraction in the soxhlet apparatus. The eeRo was separately to dichloromethane, ethyl acetate and butanol in a separator funnel to get DCM, EA and ButOH fractions, respectively. The quantification of constituents of eeRo and its fractions were made by HPLC–DAD. The eeRo and its fractions were tested in the DPPH•- radical scavenging, total antioxidant capacity assays without tissues and they were tested in sodium nitroprusside-induced lipid peroxidation and H2DCF-DA in liver, brain and stomach of male adult wistar rats. The eeRo and its fractions were analyzed in bacteria minimum inhibitory concentration assay. Besids, eeRo and its fractions were tested in ethanol-induced gastric and intestinal lesions models. After application of these models and pretreatment with eeRo the dichlorofluorescein fluorescence, lipid peroxidation, ratio of GSH/GSSG, superoxide dismutase activity, catalase activity, myeloperoxidase activity assays were performed in stomach and intestine. Moreover, the measurement of nitrite and nitrate levels, ulcer index and histopathology tests were made only in stomach. The Na+/K+ ATPase activity was measured only in intestine. The eeRo, DCM, EA had significant total antioxidant effect and DPPH•- radical scavenging activity in vitro. The DCM and eeRo got antioxidant effects against basal levels of reactive species in the liver, stomach and brain. The eeRo and DCM protected the liver and brain against lipid peroxidation induced by sodium nitroprussiate. The eeRo, DCM, EA and ButOH had inhibitory effect in the gram (+) and gram (-) bacteria. However, the eeRo and DCM, had the lower minimum inhibitory concentration.The eeRo, after in vivo treatment protected stomach and intestine against the lipid peroxidation and they increased the CAT activity. The eeRo prevented the reduction in Na+/K+ ATPase and cased the increase of superoxide dismutase activity in intestine. In addition, eeRo reduced the myeloperoxidase activity in stomach and intestine. The results of this tese suggest that the eeRo, in general way, represented the better option as agent against oxidative stress in vitro, ex vivo e mainly in the prevention of gastrointestinal lesions in association to anti-inflamatory or vasodilatory mechanisms. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-03-18 |
dc.date.accessioned.fl_str_mv |
2019-09-12T18:18:02Z |
dc.date.available.fl_str_mv |
2019-09-12T18:18:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/18218 |
url |
http://repositorio.ufsm.br/handle/1/18218 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
200800000002 |
dc.relation.confidence.fl_str_mv |
600 |
dc.relation.authority.fl_str_mv |
e99bf03a-61e7-46c9-97d1-a7255476d47f bd098fe4-13d5-41ad-893e-351b974930e7 658a0796-2a30-4f4c-84ca-d7562143b855 276bcfc2-822c-4d05-88f0-be6c067a20c1 d7dc21db-e508-4e38-b2e9-c73d2ec65927 2692a8bc-fe74-4278-b83e-83c3d8a4024d |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Bioquímica |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
bitstream.url.fl_str_mv |
http://repositorio.ufsm.br/bitstream/1/18218/2/license_rdf http://repositorio.ufsm.br/bitstream/1/18218/1/TES_PPGBT_2016_AMARAL_GUILHERME.pdf http://repositorio.ufsm.br/bitstream/1/18218/3/license.txt http://repositorio.ufsm.br/bitstream/1/18218/4/TES_PPGBT_2016_AMARAL_GUILHERME.pdf.txt http://repositorio.ufsm.br/bitstream/1/18218/5/TES_PPGBT_2016_AMARAL_GUILHERME.pdf.jpg |
bitstream.checksum.fl_str_mv |
4460e5956bc1d1639be9ae6146a50347 7569d61a27eb130a53e75b4943188075 2f0571ecee68693bd5cd3f17c1e075df 2c462a6ea051f00955d1f8fa75be62cf b37a8c0d5ca841e9cd936cd71a6a553b |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
|
_version_ |
1794523804727246848 |