Via anti-inflamatória colinérgica e proteínas de fase aguda na tripanotolerância de coelhos infectados pelo Trypanosoma. evansi
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/4084 |
Resumo: | Trypanosomiasis is a disease of worldwide distribution which in Brazil is called "mal das cadeiras", owing the clinical signs shown by horses, which are the major species affected. The disease is caused by the protozoan Trypanosoma evansi, characterized by clinical signs such as weight loss, pale mucous membranes, swelling of the eyelid and vulva. Rabbits have been reported as a resistant species to T. evansi, however, is not yet known how immune aspects, such as innate immune and cholinergic system, behave in this species, not even as this inflammations pathways influence in rabbits's trypanotolerance. Thus, the aim of this study was to investigate the inflammatory response and cholinergic anti-inflammatory pathway and its relation to trypanotolerance in rabbits infected with T. evansi. For this, twelve female adult New Zealand rabbits, weighing 3.6 4.5 kg were used in this study. The animals were divided into two groups, a control group and an infected group, both with six animals. The rabbits belonging to the infected group received, intraperitoneally, 0.5 ml of rat blood containing 108 parasites per animal, while the control group received physiological solution by the same route. The experimental period reached 118 days. Blood was collected on days 0, 2, 7, 12, 27, 42, 57, 87, 102, and 118, for to determine the cholinesterases, and days 0, 5, 20, 35, 65, 95 and 118, for to evaluate blood count, total protein, serum proteinogram, immunoglobulins and acute phase proteins. There was an increase in the activity of butyrylcholinesterase (BChE), at 7th day PI, and in the activity of acetylcholinesterase (AChE), in 27th day PI. Furthermore, the infected group showed an increase in total protein and the fractions alpha, beta and gamma globulins along of experimental period. The reduction in albumin and hematocrit were observed in precise periods of experimental infection, as well as the increase in immunoglobulin G. Infection with T. evansi stimulated the production of acute phase proteins, such as C-reactive protein, haptoglobin, α-2 macroglobulin, being observed increase in immunoglobulin M (IgM) throughout the experimental period (118 days post-infection). From these results, it is concluded that the cholinergic pathway had influence the inflammatory response, through the action of AChE and BChE in the regulation of concentrations of acetylcholine, resulting in increased concentrations of cytokines and, consequently, in the production phase protein acute. The increase in IgM, associated with increased C-reactive protein and haptoglobin, suggests the involvement of these proteins in host defense against flagellated, with possible participation in trypanotolerance of rabbits infected with T. evansi. |
id |
UFSM-20_feeb716a6f355a61d9062b676a67ddd3 |
---|---|
oai_identifier_str |
oai:repositorio.ufsm.br:1/4084 |
network_acronym_str |
UFSM-20 |
network_name_str |
Manancial - Repositório Digital da UFSM |
repository_id_str |
3913 |
spelling |
2017-06-142017-06-142013-04-26COSTA, Marcio Machado. Cholinergic anti-inflammatory pathway and acute phase proteins in trypanotolerance of rabbits infected by Trypanosoma evansi. 2013. 85 f. Tese (Doutorado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2013.http://repositorio.ufsm.br/handle/1/4084Trypanosomiasis is a disease of worldwide distribution which in Brazil is called "mal das cadeiras", owing the clinical signs shown by horses, which are the major species affected. The disease is caused by the protozoan Trypanosoma evansi, characterized by clinical signs such as weight loss, pale mucous membranes, swelling of the eyelid and vulva. Rabbits have been reported as a resistant species to T. evansi, however, is not yet known how immune aspects, such as innate immune and cholinergic system, behave in this species, not even as this inflammations pathways influence in rabbits's trypanotolerance. Thus, the aim of this study was to investigate the inflammatory response and cholinergic anti-inflammatory pathway and its relation to trypanotolerance in rabbits infected with T. evansi. For this, twelve female adult New Zealand rabbits, weighing 3.6 4.5 kg were used in this study. The animals were divided into two groups, a control group and an infected group, both with six animals. The rabbits belonging to the infected group received, intraperitoneally, 0.5 ml of rat blood containing 108 parasites per animal, while the control group received physiological solution by the same route. The experimental period reached 118 days. Blood was collected on days 0, 2, 7, 12, 27, 42, 57, 87, 102, and 118, for to determine the cholinesterases, and days 0, 5, 20, 35, 65, 95 and 118, for to evaluate blood count, total protein, serum proteinogram, immunoglobulins and acute phase proteins. There was an increase in the activity of butyrylcholinesterase (BChE), at 7th day PI, and in the activity of acetylcholinesterase (AChE), in 27th day PI. Furthermore, the infected group showed an increase in total protein and the fractions alpha, beta and gamma globulins along of experimental period. The reduction in albumin and hematocrit were observed in precise periods of experimental infection, as well as the increase in immunoglobulin G. Infection with T. evansi stimulated the production of acute phase proteins, such as C-reactive protein, haptoglobin, α-2 macroglobulin, being observed increase in immunoglobulin M (IgM) throughout the experimental period (118 days post-infection). From these results, it is concluded that the cholinergic pathway had influence the inflammatory response, through the action of AChE and BChE in the regulation of concentrations of acetylcholine, resulting in increased concentrations of cytokines and, consequently, in the production phase protein acute. The increase in IgM, associated with increased C-reactive protein and haptoglobin, suggests the involvement of these proteins in host defense against flagellated, with possible participation in trypanotolerance of rabbits infected with T. evansi.A tripanossomose é uma enfermidade de distribuição mundial que, no Brasil, é denominada mal das cadeiras , em função dos sinais clínicos apresentados pelos equinos, principal espécie afetada. A doença é causada pelo protozoário Trypanosoma evansi, sendo caracterizada por sinais clínicos como perda de peso, mucosas pálidas, edema de pálpebra e vulva. Coelhos têm sido relatados como uma espécie resistente ao T. evansi, contudo, ainda não se sabe de que modo aspectos imunitários, como a imunidade inata e o sistema colinérgico, comportam-se nessa espécie, nem mesmo como essas vias da inflamação influenciam a tripanotolerância de coelhos. Assim, o objetivo deste trabalho foi investigar a resposta inflamatória e a via anti-inflamatória colinérgica e sua relação na tripanotolerância em coelhos infectados experimentalmente pelo T. evansi. Para tanto, foram utilizados 12 coelhos adultos, fêmeas, da raça Nova Zelândia, com peso corporal entre 3,6 a 4,5 Kg, divididos em dois grupos, um grupo controle e um grupo infectado, ambos com seis animais. Os coelhos pertencentes ao grupo infectado receberam, pela via intraperitoneal, 0,5 mL de sangue de rato contendo 108 tripanossomas por animal, enquanto que o grupo controle recebeu, pela mesma via, solução fisiológica. O período experimental foi de 118 dias, sendo o sangue coletado nos dias 0, 2, 7, 12, 27, 42, 57, 87, 102, e 118, para determinar as colinesterases, e nos dias 0, 5, 20, 35, 65, 95 e 118, para avaliar hemograma, proteínas totais, proteinograma sérico, imunoglobulinas e proteínas de fase aguda. Foi observado aumento na atividade da butirilcolinesterase (BChE) no 7° dia PI e da atividade da acetilcolinesterase (AChE) no 27° dia PI. Além disso, o grupo infectado apresentou aumento nas proteínas totais, bem como nas frações alfa, beta e gama globulinas ao longo do período experimental. A redução na albumina e no hematócrito foram observados em períodos pontuais da infecção experimental, bem como o aumento na imunoglobulina G. A infecção pelo T. evansi estimulou a produção de proteínas de fase aguda como a proteína C-reativa, haptoglobina, α-2 macroglobulina, sendo observado um aumento na imunoglobulina M (IgM) em todo o período experimental (118 dias pós-infecção). A partir desses resultados, conclui-se que a via colinérgica pode ter influência na resposta inflamatória, através da ação da BChE e AChE na regulação das concentrações de acetilcolina, acarretando em aumento nas concentrações de citocinas e, consequentemente, na produção de proteínas de fase aguda. A elevação na IgM, relacionada ao aumento da proteína C-reativa e haptoglobina, sugere o envolvimento dessas proteínas na defesa do hospedeiro contra o flagelado, e, possivelmente, na tripanotolerância de coelhos infectados com T. evansi.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Medicina VeterináriaUFSMBRMedicina VeterináriaProteínas de fase agudaColinesteraseImunoglobulina MTripanotolerânciaAcute phase proteinsCholinesterasesImmunoglobulin MTrypanotoleranceCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAVia anti-inflamatória colinérgica e proteínas de fase aguda na tripanotolerância de coelhos infectados pelo Trypanosoma. evansiCholinergic anti-inflammatory pathway and acute phase proteins in trypanotolerance of rabbits infected by Trypanosoma evansiinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisMonteiro, Silvia Gonzalezhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4700451Z9Silva, Aleksandro Schafer dahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4559506T7Anjos, Bruno Leite doshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4710215Z7Leal, Daniela Bitencourt Rosahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4706487E7Mazzanti, Cinthia Melazzo Andradehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4776635P4http://lattes.cnpq.br/2589503658638251Costa, Marcio Machado500500000007400300300500300300300f658c142-7522-4cd5-a133-f2479652bf3135b2c6d7-bfd4-41cb-aeeb-e058fa9f383befc957bd-44d9-4333-83a3-eb1b550d7b862caf7c2b-75ff-49ff-8ec8-716ce44d027957c82517-f994-4a9f-abc3-df1ffe4a214db8939eb5-9407-4207-b8dd-f3bbebdeec94info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALCOSTA, MARCIO MACHADO2.pdfapplication/pdf1334489http://repositorio.ufsm.br/bitstream/1/4084/1/COSTA%2c%20MARCIO%20MACHADO2.pdf2ba1299c9401a9b21fb1e6b3b0056977MD51TEXTCOSTA, MARCIO MACHADO2.pdf.txtCOSTA, MARCIO MACHADO2.pdf.txtExtracted texttext/plain134077http://repositorio.ufsm.br/bitstream/1/4084/2/COSTA%2c%20MARCIO%20MACHADO2.pdf.txt48b183b1d6aa68be04f8aafb8343865dMD52THUMBNAILCOSTA, MARCIO MACHADO2.pdf.jpgCOSTA, MARCIO MACHADO2.pdf.jpgIM Thumbnailimage/jpeg5107http://repositorio.ufsm.br/bitstream/1/4084/3/COSTA%2c%20MARCIO%20MACHADO2.pdf.jpg2ce25badc05e4d66d635836f3d09d1e1MD531/40842022-08-29 11:19:00.106oai:repositorio.ufsm.br:1/4084Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestopendoar:39132022-08-29T14:19Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Via anti-inflamatória colinérgica e proteínas de fase aguda na tripanotolerância de coelhos infectados pelo Trypanosoma. evansi |
dc.title.alternative.eng.fl_str_mv |
Cholinergic anti-inflammatory pathway and acute phase proteins in trypanotolerance of rabbits infected by Trypanosoma evansi |
title |
Via anti-inflamatória colinérgica e proteínas de fase aguda na tripanotolerância de coelhos infectados pelo Trypanosoma. evansi |
spellingShingle |
Via anti-inflamatória colinérgica e proteínas de fase aguda na tripanotolerância de coelhos infectados pelo Trypanosoma. evansi Costa, Marcio Machado Proteínas de fase aguda Colinesterase Imunoglobulina M Tripanotolerância Acute phase proteins Cholinesterases Immunoglobulin M Trypanotolerance CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Via anti-inflamatória colinérgica e proteínas de fase aguda na tripanotolerância de coelhos infectados pelo Trypanosoma. evansi |
title_full |
Via anti-inflamatória colinérgica e proteínas de fase aguda na tripanotolerância de coelhos infectados pelo Trypanosoma. evansi |
title_fullStr |
Via anti-inflamatória colinérgica e proteínas de fase aguda na tripanotolerância de coelhos infectados pelo Trypanosoma. evansi |
title_full_unstemmed |
Via anti-inflamatória colinérgica e proteínas de fase aguda na tripanotolerância de coelhos infectados pelo Trypanosoma. evansi |
title_sort |
Via anti-inflamatória colinérgica e proteínas de fase aguda na tripanotolerância de coelhos infectados pelo Trypanosoma. evansi |
author |
Costa, Marcio Machado |
author_facet |
Costa, Marcio Machado |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Monteiro, Silvia Gonzalez |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4700451Z9 |
dc.contributor.referee1.fl_str_mv |
Silva, Aleksandro Schafer da |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4559506T7 |
dc.contributor.referee2.fl_str_mv |
Anjos, Bruno Leite dos |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4710215Z7 |
dc.contributor.referee3.fl_str_mv |
Leal, Daniela Bitencourt Rosa |
dc.contributor.referee3Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4706487E7 |
dc.contributor.referee4.fl_str_mv |
Mazzanti, Cinthia Melazzo Andrade |
dc.contributor.referee4Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4776635P4 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2589503658638251 |
dc.contributor.author.fl_str_mv |
Costa, Marcio Machado |
contributor_str_mv |
Monteiro, Silvia Gonzalez Silva, Aleksandro Schafer da Anjos, Bruno Leite dos Leal, Daniela Bitencourt Rosa Mazzanti, Cinthia Melazzo Andrade |
dc.subject.por.fl_str_mv |
Proteínas de fase aguda Colinesterase Imunoglobulina M Tripanotolerância |
topic |
Proteínas de fase aguda Colinesterase Imunoglobulina M Tripanotolerância Acute phase proteins Cholinesterases Immunoglobulin M Trypanotolerance CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Acute phase proteins Cholinesterases Immunoglobulin M Trypanotolerance |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
Trypanosomiasis is a disease of worldwide distribution which in Brazil is called "mal das cadeiras", owing the clinical signs shown by horses, which are the major species affected. The disease is caused by the protozoan Trypanosoma evansi, characterized by clinical signs such as weight loss, pale mucous membranes, swelling of the eyelid and vulva. Rabbits have been reported as a resistant species to T. evansi, however, is not yet known how immune aspects, such as innate immune and cholinergic system, behave in this species, not even as this inflammations pathways influence in rabbits's trypanotolerance. Thus, the aim of this study was to investigate the inflammatory response and cholinergic anti-inflammatory pathway and its relation to trypanotolerance in rabbits infected with T. evansi. For this, twelve female adult New Zealand rabbits, weighing 3.6 4.5 kg were used in this study. The animals were divided into two groups, a control group and an infected group, both with six animals. The rabbits belonging to the infected group received, intraperitoneally, 0.5 ml of rat blood containing 108 parasites per animal, while the control group received physiological solution by the same route. The experimental period reached 118 days. Blood was collected on days 0, 2, 7, 12, 27, 42, 57, 87, 102, and 118, for to determine the cholinesterases, and days 0, 5, 20, 35, 65, 95 and 118, for to evaluate blood count, total protein, serum proteinogram, immunoglobulins and acute phase proteins. There was an increase in the activity of butyrylcholinesterase (BChE), at 7th day PI, and in the activity of acetylcholinesterase (AChE), in 27th day PI. Furthermore, the infected group showed an increase in total protein and the fractions alpha, beta and gamma globulins along of experimental period. The reduction in albumin and hematocrit were observed in precise periods of experimental infection, as well as the increase in immunoglobulin G. Infection with T. evansi stimulated the production of acute phase proteins, such as C-reactive protein, haptoglobin, α-2 macroglobulin, being observed increase in immunoglobulin M (IgM) throughout the experimental period (118 days post-infection). From these results, it is concluded that the cholinergic pathway had influence the inflammatory response, through the action of AChE and BChE in the regulation of concentrations of acetylcholine, resulting in increased concentrations of cytokines and, consequently, in the production phase protein acute. The increase in IgM, associated with increased C-reactive protein and haptoglobin, suggests the involvement of these proteins in host defense against flagellated, with possible participation in trypanotolerance of rabbits infected with T. evansi. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-04-26 |
dc.date.accessioned.fl_str_mv |
2017-06-14 |
dc.date.available.fl_str_mv |
2017-06-14 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
COSTA, Marcio Machado. Cholinergic anti-inflammatory pathway and acute phase proteins in trypanotolerance of rabbits infected by Trypanosoma evansi. 2013. 85 f. Tese (Doutorado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2013. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/4084 |
identifier_str_mv |
COSTA, Marcio Machado. Cholinergic anti-inflammatory pathway and acute phase proteins in trypanotolerance of rabbits infected by Trypanosoma evansi. 2013. 85 f. Tese (Doutorado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2013. |
url |
http://repositorio.ufsm.br/handle/1/4084 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
500500000007 |
dc.relation.confidence.fl_str_mv |
400 300 300 500 300 300 300 |
dc.relation.authority.fl_str_mv |
f658c142-7522-4cd5-a133-f2479652bf31 35b2c6d7-bfd4-41cb-aeeb-e058fa9f383b efc957bd-44d9-4333-83a3-eb1b550d7b86 2caf7c2b-75ff-49ff-8ec8-716ce44d0279 57c82517-f994-4a9f-abc3-df1ffe4a214d b8939eb5-9407-4207-b8dd-f3bbebdeec94 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Medicina Veterinária |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Medicina Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
bitstream.url.fl_str_mv |
http://repositorio.ufsm.br/bitstream/1/4084/1/COSTA%2c%20MARCIO%20MACHADO2.pdf http://repositorio.ufsm.br/bitstream/1/4084/2/COSTA%2c%20MARCIO%20MACHADO2.pdf.txt http://repositorio.ufsm.br/bitstream/1/4084/3/COSTA%2c%20MARCIO%20MACHADO2.pdf.jpg |
bitstream.checksum.fl_str_mv |
2ba1299c9401a9b21fb1e6b3b0056977 48b183b1d6aa68be04f8aafb8343865d 2ce25badc05e4d66d635836f3d09d1e1 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
|
_version_ |
1794523880836038656 |