Culture medium and inoculation methodology for the study of soft root rot caused by Phytopythium sp.

Bibliographic Details
Main Author: Silva,Jonny Lucio de Sousa
Publication Date: 2019
Other Authors: Ishida,Alessandra Keiko Nakasone, Cunha,Roberto Lisboa, Lima,Aline Medeiros, Moura,Elisa Ferreira
Format: Article
Language: eng
Source: Ciência Rural
Download full: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019001100401
Summary: ABSTRACT: Cassava (Manihot esculenta Crantz) is an important crop in Brazil and Pará is the major producer of roots. High temperature and humidity of tropical regions favor the development of various diseases, among them the cassava root rot. The objective of this study was to evaluate the effect of luminosity and culture medium on the mycelial growth and sporulation of Phytopythium sp. associated with different methods of inoculation on cassava roots. In vitro tests for pathogen growth were established in a 2 x 6 factorial design (luminosity x culture medium) with five replicates and the means were compared by t test (P≤0.05). The culture medium containing sweet cassava root produced greater mycelial development and higher pathogen sporulation and it was the most suitable medium for pathogen culture. The culture under absence of light generated better mycelial growth than culture under 12 hour of light. Regarding the type of inoculation, the response was better when deeper injuries were induced.
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spelling Culture medium and inoculation methodology for the study of soft root rot caused by Phytopythium sp.oomycetesporulationmyceliumABSTRACT: Cassava (Manihot esculenta Crantz) is an important crop in Brazil and Pará is the major producer of roots. High temperature and humidity of tropical regions favor the development of various diseases, among them the cassava root rot. The objective of this study was to evaluate the effect of luminosity and culture medium on the mycelial growth and sporulation of Phytopythium sp. associated with different methods of inoculation on cassava roots. In vitro tests for pathogen growth were established in a 2 x 6 factorial design (luminosity x culture medium) with five replicates and the means were compared by t test (P≤0.05). The culture medium containing sweet cassava root produced greater mycelial development and higher pathogen sporulation and it was the most suitable medium for pathogen culture. The culture under absence of light generated better mycelial growth than culture under 12 hour of light. Regarding the type of inoculation, the response was better when deeper injuries were induced.Universidade Federal de Santa Maria2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019001100401Ciência Rural v.49 n.11 2019reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20180875info:eu-repo/semantics/openAccessSilva,Jonny Lucio de SousaIshida,Alessandra Keiko NakasoneCunha,Roberto LisboaLima,Aline MedeirosMoura,Elisa Ferreiraeng2019-10-04T00:00:00ZRevista
dc.title.none.fl_str_mv Culture medium and inoculation methodology for the study of soft root rot caused by Phytopythium sp.
title Culture medium and inoculation methodology for the study of soft root rot caused by Phytopythium sp.
spellingShingle Culture medium and inoculation methodology for the study of soft root rot caused by Phytopythium sp.
Silva,Jonny Lucio de Sousa
oomycete
sporulation
mycelium
title_short Culture medium and inoculation methodology for the study of soft root rot caused by Phytopythium sp.
title_full Culture medium and inoculation methodology for the study of soft root rot caused by Phytopythium sp.
title_fullStr Culture medium and inoculation methodology for the study of soft root rot caused by Phytopythium sp.
title_full_unstemmed Culture medium and inoculation methodology for the study of soft root rot caused by Phytopythium sp.
title_sort Culture medium and inoculation methodology for the study of soft root rot caused by Phytopythium sp.
author Silva,Jonny Lucio de Sousa
author_facet Silva,Jonny Lucio de Sousa
Ishida,Alessandra Keiko Nakasone
Cunha,Roberto Lisboa
Lima,Aline Medeiros
Moura,Elisa Ferreira
author_role author
author2 Ishida,Alessandra Keiko Nakasone
Cunha,Roberto Lisboa
Lima,Aline Medeiros
Moura,Elisa Ferreira
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Silva,Jonny Lucio de Sousa
Ishida,Alessandra Keiko Nakasone
Cunha,Roberto Lisboa
Lima,Aline Medeiros
Moura,Elisa Ferreira
dc.subject.por.fl_str_mv oomycete
sporulation
mycelium
topic oomycete
sporulation
mycelium
description ABSTRACT: Cassava (Manihot esculenta Crantz) is an important crop in Brazil and Pará is the major producer of roots. High temperature and humidity of tropical regions favor the development of various diseases, among them the cassava root rot. The objective of this study was to evaluate the effect of luminosity and culture medium on the mycelial growth and sporulation of Phytopythium sp. associated with different methods of inoculation on cassava roots. In vitro tests for pathogen growth were established in a 2 x 6 factorial design (luminosity x culture medium) with five replicates and the means were compared by t test (P≤0.05). The culture medium containing sweet cassava root produced greater mycelial development and higher pathogen sporulation and it was the most suitable medium for pathogen culture. The culture under absence of light generated better mycelial growth than culture under 12 hour of light. Regarding the type of inoculation, the response was better when deeper injuries were induced.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019001100401
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019001100401
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0103-8478cr20180875
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.49 n.11 2019
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
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repository.mail.fl_str_mv
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