Staphylococcal cassette chromosome mec elements from Staphylococcus intermedius group (SIG) isolates from dogs in a center for veterinary diagnostics in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782021000900451 |
Resumo: | ABSTRACT: Methicillin resistance in the Staphylococcus intermedius group (SIG) has emerged in small animal practice. Methicillin-resistant SIG (MRSIG) members have been implicated as causes of infections in both companion animals and humans. Staphylococcal cassette chromosome mec (SCCmec) elements carry the mecA/C genes, which encode for the transpeptidase PBP2a (PBP2’) responsible for β-lactam antibiotic resistance in staphylococci. This study examined the SCCmec types of MRSIG isolates from different clinical specimens of dogs that exhibited methicillin MIC ≥ 0.5 μg/mL by an automated identification and susceptibility system in a Center for Veterinary Diagnostics in São Paulo, Brazil. Susceptibility to methicillin was determined by broth microdilution testing, and Oxoid® M.I.C.Evaluator® strips. PBP2a production was detected using a latex agglutination assay. SCCmec typing was performed according to the International Working Group on the Classification of Staphylococcal Cassette Chromosome Elements (IWG-SCC) guidelines. SCCmec type II (2A), SCCmec type III (3A), composite SCC structures consisting of a class A mec gene complex in addition to multiple ccr gene complexes, and non-typable SCCmec elements were reported in these MRSIG isolates. SCCmec type variants differing from those so far acknowledged by IWG-SCC were found, indicating new rearrangements in the genetic context of mecA in these canine MRSIG isolates. |
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Ciência rural (Online) |
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Staphylococcal cassette chromosome mec elements from Staphylococcus intermedius group (SIG) isolates from dogs in a center for veterinary diagnostics in BrazilStaphylococcus intermedius group (SIG)methicillin resistanceSCCmec elementscompanion animals.ABSTRACT: Methicillin resistance in the Staphylococcus intermedius group (SIG) has emerged in small animal practice. Methicillin-resistant SIG (MRSIG) members have been implicated as causes of infections in both companion animals and humans. Staphylococcal cassette chromosome mec (SCCmec) elements carry the mecA/C genes, which encode for the transpeptidase PBP2a (PBP2’) responsible for β-lactam antibiotic resistance in staphylococci. This study examined the SCCmec types of MRSIG isolates from different clinical specimens of dogs that exhibited methicillin MIC ≥ 0.5 μg/mL by an automated identification and susceptibility system in a Center for Veterinary Diagnostics in São Paulo, Brazil. Susceptibility to methicillin was determined by broth microdilution testing, and Oxoid® M.I.C.Evaluator® strips. PBP2a production was detected using a latex agglutination assay. SCCmec typing was performed according to the International Working Group on the Classification of Staphylococcal Cassette Chromosome Elements (IWG-SCC) guidelines. SCCmec type II (2A), SCCmec type III (3A), composite SCC structures consisting of a class A mec gene complex in addition to multiple ccr gene complexes, and non-typable SCCmec elements were reported in these MRSIG isolates. SCCmec type variants differing from those so far acknowledged by IWG-SCC were found, indicating new rearrangements in the genetic context of mecA in these canine MRSIG isolates.Universidade Federal de Santa Maria2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782021000900451Ciência Rural v.51 n.9 2021reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20200936info:eu-repo/semantics/openAccessRuiz,ReginaBarbosa,Valcilaine TeixeiraLôbo,Teresa de Lisieux Guedes FerreiraSilva,Rafael Aleixo dos SantosCalumby,Rodrigo José NunesVieira,Suzanne PinheiroBrandão,DiogoZorzi,Felipe MendesMoreira,Rossana Teotônio de FariasAlvino,ValterNascimento,Ticiano Gomes doGrilo,Luciano Aparecido MeirelesDornelas,Camila BragaMamizuka,Elsa MasaeAlmeida,Lara Mendes deeng2021-05-31T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Staphylococcal cassette chromosome mec elements from Staphylococcus intermedius group (SIG) isolates from dogs in a center for veterinary diagnostics in Brazil |
title |
Staphylococcal cassette chromosome mec elements from Staphylococcus intermedius group (SIG) isolates from dogs in a center for veterinary diagnostics in Brazil |
spellingShingle |
Staphylococcal cassette chromosome mec elements from Staphylococcus intermedius group (SIG) isolates from dogs in a center for veterinary diagnostics in Brazil Ruiz,Regina Staphylococcus intermedius group (SIG) methicillin resistance SCCmec elements companion animals. |
title_short |
Staphylococcal cassette chromosome mec elements from Staphylococcus intermedius group (SIG) isolates from dogs in a center for veterinary diagnostics in Brazil |
title_full |
Staphylococcal cassette chromosome mec elements from Staphylococcus intermedius group (SIG) isolates from dogs in a center for veterinary diagnostics in Brazil |
title_fullStr |
Staphylococcal cassette chromosome mec elements from Staphylococcus intermedius group (SIG) isolates from dogs in a center for veterinary diagnostics in Brazil |
title_full_unstemmed |
Staphylococcal cassette chromosome mec elements from Staphylococcus intermedius group (SIG) isolates from dogs in a center for veterinary diagnostics in Brazil |
title_sort |
Staphylococcal cassette chromosome mec elements from Staphylococcus intermedius group (SIG) isolates from dogs in a center for veterinary diagnostics in Brazil |
author |
Ruiz,Regina |
author_facet |
Ruiz,Regina Barbosa,Valcilaine Teixeira Lôbo,Teresa de Lisieux Guedes Ferreira Silva,Rafael Aleixo dos Santos Calumby,Rodrigo José Nunes Vieira,Suzanne Pinheiro Brandão,Diogo Zorzi,Felipe Mendes Moreira,Rossana Teotônio de Farias Alvino,Valter Nascimento,Ticiano Gomes do Grilo,Luciano Aparecido Meireles Dornelas,Camila Braga Mamizuka,Elsa Masae Almeida,Lara Mendes de |
author_role |
author |
author2 |
Barbosa,Valcilaine Teixeira Lôbo,Teresa de Lisieux Guedes Ferreira Silva,Rafael Aleixo dos Santos Calumby,Rodrigo José Nunes Vieira,Suzanne Pinheiro Brandão,Diogo Zorzi,Felipe Mendes Moreira,Rossana Teotônio de Farias Alvino,Valter Nascimento,Ticiano Gomes do Grilo,Luciano Aparecido Meireles Dornelas,Camila Braga Mamizuka,Elsa Masae Almeida,Lara Mendes de |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Ruiz,Regina Barbosa,Valcilaine Teixeira Lôbo,Teresa de Lisieux Guedes Ferreira Silva,Rafael Aleixo dos Santos Calumby,Rodrigo José Nunes Vieira,Suzanne Pinheiro Brandão,Diogo Zorzi,Felipe Mendes Moreira,Rossana Teotônio de Farias Alvino,Valter Nascimento,Ticiano Gomes do Grilo,Luciano Aparecido Meireles Dornelas,Camila Braga Mamizuka,Elsa Masae Almeida,Lara Mendes de |
dc.subject.por.fl_str_mv |
Staphylococcus intermedius group (SIG) methicillin resistance SCCmec elements companion animals. |
topic |
Staphylococcus intermedius group (SIG) methicillin resistance SCCmec elements companion animals. |
description |
ABSTRACT: Methicillin resistance in the Staphylococcus intermedius group (SIG) has emerged in small animal practice. Methicillin-resistant SIG (MRSIG) members have been implicated as causes of infections in both companion animals and humans. Staphylococcal cassette chromosome mec (SCCmec) elements carry the mecA/C genes, which encode for the transpeptidase PBP2a (PBP2’) responsible for β-lactam antibiotic resistance in staphylococci. This study examined the SCCmec types of MRSIG isolates from different clinical specimens of dogs that exhibited methicillin MIC ≥ 0.5 μg/mL by an automated identification and susceptibility system in a Center for Veterinary Diagnostics in São Paulo, Brazil. Susceptibility to methicillin was determined by broth microdilution testing, and Oxoid® M.I.C.Evaluator® strips. PBP2a production was detected using a latex agglutination assay. SCCmec typing was performed according to the International Working Group on the Classification of Staphylococcal Cassette Chromosome Elements (IWG-SCC) guidelines. SCCmec type II (2A), SCCmec type III (3A), composite SCC structures consisting of a class A mec gene complex in addition to multiple ccr gene complexes, and non-typable SCCmec elements were reported in these MRSIG isolates. SCCmec type variants differing from those so far acknowledged by IWG-SCC were found, indicating new rearrangements in the genetic context of mecA in these canine MRSIG isolates. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782021000900451 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782021000900451 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-8478cr20200936 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.51 n.9 2021 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140556120850432 |