Induction and identification of polyploids in Cattleya intermedia Lindl. (orchidaceae) by in vitro techniques
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782000000100017 |
Resumo: | The effect of colchicine on the protocorm-like bodies of Cattleya intermedia L. (Orchidaceae) cultured in vitro was studied. Different concentrations of the drug (0.00, 0.05, 0.10 and 0.20%) as well as two times of culture (4 or 8 days) were tested to determine the best treatment for the induction of tetraploid plants. Of the 3 colchicine levels applied, 0.05 and 0.10% of drug treatments appeared to be effective on the production of mixoploids and tetraploids and could be further used in breeding programs. The suitability of stomatal area and stomata density as criteria for the distinction between diploids and tetraploids was also tested. The results suggested that tetraploid plants could be identified with a fair amount of certainty when the screening was based on the density of stomata. |
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Induction and identification of polyploids in Cattleya intermedia Lindl. (orchidaceae) by in vitro techniquesCattleyacolchicinechromosome numberorchid improvementpolyploidystomata analysisThe effect of colchicine on the protocorm-like bodies of Cattleya intermedia L. (Orchidaceae) cultured in vitro was studied. Different concentrations of the drug (0.00, 0.05, 0.10 and 0.20%) as well as two times of culture (4 or 8 days) were tested to determine the best treatment for the induction of tetraploid plants. Of the 3 colchicine levels applied, 0.05 and 0.10% of drug treatments appeared to be effective on the production of mixoploids and tetraploids and could be further used in breeding programs. The suitability of stomatal area and stomata density as criteria for the distinction between diploids and tetraploids was also tested. The results suggested that tetraploid plants could be identified with a fair amount of certainty when the screening was based on the density of stomata.Universidade Federal de Santa Maria2000-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782000000100017Ciência Rural v.30 n.1 2000reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/S0103-84782000000100017info:eu-repo/semantics/openAccessSilva,Paulo Artur Konzen Xavier de Mello eCallegari-Jacques,SídiaBodanese-Zanettini,Maria Helenaeng2006-12-08T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Induction and identification of polyploids in Cattleya intermedia Lindl. (orchidaceae) by in vitro techniques |
title |
Induction and identification of polyploids in Cattleya intermedia Lindl. (orchidaceae) by in vitro techniques |
spellingShingle |
Induction and identification of polyploids in Cattleya intermedia Lindl. (orchidaceae) by in vitro techniques Silva,Paulo Artur Konzen Xavier de Mello e Cattleya colchicine chromosome number orchid improvement polyploidy stomata analysis |
title_short |
Induction and identification of polyploids in Cattleya intermedia Lindl. (orchidaceae) by in vitro techniques |
title_full |
Induction and identification of polyploids in Cattleya intermedia Lindl. (orchidaceae) by in vitro techniques |
title_fullStr |
Induction and identification of polyploids in Cattleya intermedia Lindl. (orchidaceae) by in vitro techniques |
title_full_unstemmed |
Induction and identification of polyploids in Cattleya intermedia Lindl. (orchidaceae) by in vitro techniques |
title_sort |
Induction and identification of polyploids in Cattleya intermedia Lindl. (orchidaceae) by in vitro techniques |
author |
Silva,Paulo Artur Konzen Xavier de Mello e |
author_facet |
Silva,Paulo Artur Konzen Xavier de Mello e Callegari-Jacques,Sídia Bodanese-Zanettini,Maria Helena |
author_role |
author |
author2 |
Callegari-Jacques,Sídia Bodanese-Zanettini,Maria Helena |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Silva,Paulo Artur Konzen Xavier de Mello e Callegari-Jacques,Sídia Bodanese-Zanettini,Maria Helena |
dc.subject.por.fl_str_mv |
Cattleya colchicine chromosome number orchid improvement polyploidy stomata analysis |
topic |
Cattleya colchicine chromosome number orchid improvement polyploidy stomata analysis |
description |
The effect of colchicine on the protocorm-like bodies of Cattleya intermedia L. (Orchidaceae) cultured in vitro was studied. Different concentrations of the drug (0.00, 0.05, 0.10 and 0.20%) as well as two times of culture (4 or 8 days) were tested to determine the best treatment for the induction of tetraploid plants. Of the 3 colchicine levels applied, 0.05 and 0.10% of drug treatments appeared to be effective on the production of mixoploids and tetraploids and could be further used in breeding programs. The suitability of stomatal area and stomata density as criteria for the distinction between diploids and tetraploids was also tested. The results suggested that tetraploid plants could be identified with a fair amount of certainty when the screening was based on the density of stomata. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782000000100017 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782000000100017 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-84782000000100017 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.30 n.1 2000 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140521193832448 |