Vacuum-cooled liquid nitrogen increases the developmental ability of vitrified-warmed bovine oocytes
Autor(a) principal: | |
---|---|
Data de Publicação: | 2006 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000500024 |
Resumo: | The objective of this study was to determine the effects of vacuum-cooled liquid nitrogen on the development of vitrified immature (germinal vesicle stage; GV) and mature (metaphase II; MII) bovine oocytes after re-warming. Liquid nitrogen was exposed to either atmospheric pressure or to a vacuum (300mm Hg for 45sec); the latter decreased the temperature of the liquid nitrogen to -200°C. Partially denuded oocytes were vitrified either just after selection (GV) or after 22 hours of in vitro maturation (MII) in TCM 199 medium + 10% of estrous mare serum. For vitrification, oocytes were firstly exposed to an intermediate solution (10% EG + 10% DMSO) for 30sec, followed by the vitrification solution (20% EG + 20% DMSO + 0.5M sucrose) for 20sec. Groups of three or four oocytes were loaded into an open-pulled-straw and directly plunged into liquid nitrogen. Oocytes were subsequently re-warmed by exposure to air (25°C) for 4sec, followed by 5 min exposure to decreasing concentrations (0.3 and 0.15M) of sucrose. Fertilization (Day 0) was done with 2 x 106 spermatozoa mL-1 (selected by a swim-up procedure) and incubated for 18 to 22 hours. Presumptive zygotes were cultured at 39°C in four-well dishes with SOFaaci medium, under 5% CO2 and saturated humidity. Cleavage (Day 2) and blastocyst rates (Day 8) were 33.9 and 4.2%, respectively, for GV stage oocytes at atmospheric pressure, 41.2 and 8.8% for GV oocytes under vacuum, 43.5 and 6.7% for MII oocytes at atmospheric pressure, and 53.6 and 10.6% for MII oocytes under vacuum. In conclusion, vacuum-cooled liquid nitrogen improved developmental rates of vitrified-thawed bovine oocytes. |
id |
UFSM-2_79fddddc4447c88862771e554fa89ca6 |
---|---|
oai_identifier_str |
oai:scielo:S0103-84782006000500024 |
network_acronym_str |
UFSM-2 |
network_name_str |
Ciência rural (Online) |
repository_id_str |
|
spelling |
Vacuum-cooled liquid nitrogen increases the developmental ability of vitrified-warmed bovine oocytesvitrificationcryopreservationoocytesbovinevacuumThe objective of this study was to determine the effects of vacuum-cooled liquid nitrogen on the development of vitrified immature (germinal vesicle stage; GV) and mature (metaphase II; MII) bovine oocytes after re-warming. Liquid nitrogen was exposed to either atmospheric pressure or to a vacuum (300mm Hg for 45sec); the latter decreased the temperature of the liquid nitrogen to -200°C. Partially denuded oocytes were vitrified either just after selection (GV) or after 22 hours of in vitro maturation (MII) in TCM 199 medium + 10% of estrous mare serum. For vitrification, oocytes were firstly exposed to an intermediate solution (10% EG + 10% DMSO) for 30sec, followed by the vitrification solution (20% EG + 20% DMSO + 0.5M sucrose) for 20sec. Groups of three or four oocytes were loaded into an open-pulled-straw and directly plunged into liquid nitrogen. Oocytes were subsequently re-warmed by exposure to air (25°C) for 4sec, followed by 5 min exposure to decreasing concentrations (0.3 and 0.15M) of sucrose. Fertilization (Day 0) was done with 2 x 106 spermatozoa mL-1 (selected by a swim-up procedure) and incubated for 18 to 22 hours. Presumptive zygotes were cultured at 39°C in four-well dishes with SOFaaci medium, under 5% CO2 and saturated humidity. Cleavage (Day 2) and blastocyst rates (Day 8) were 33.9 and 4.2%, respectively, for GV stage oocytes at atmospheric pressure, 41.2 and 8.8% for GV oocytes under vacuum, 43.5 and 6.7% for MII oocytes at atmospheric pressure, and 53.6 and 10.6% for MII oocytes under vacuum. In conclusion, vacuum-cooled liquid nitrogen improved developmental rates of vitrified-thawed bovine oocytes.Universidade Federal de Santa Maria2006-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000500024Ciência Rural v.36 n.5 2006reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/S0103-84782006000500024info:eu-repo/semantics/openAccessSantos,Rodrigo Marques dosBarreta,Marcos HenriqueFrajblat,MarcelCucco,Diego CórdovaMezzalira,Joana ClaudiaBunn,SilvérioCruz,Fabiano BussVieira,Arnaldo DinizMezzalira,Alceueng2006-09-04T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Vacuum-cooled liquid nitrogen increases the developmental ability of vitrified-warmed bovine oocytes |
title |
Vacuum-cooled liquid nitrogen increases the developmental ability of vitrified-warmed bovine oocytes |
spellingShingle |
Vacuum-cooled liquid nitrogen increases the developmental ability of vitrified-warmed bovine oocytes Santos,Rodrigo Marques dos vitrification cryopreservation oocytes bovine vacuum |
title_short |
Vacuum-cooled liquid nitrogen increases the developmental ability of vitrified-warmed bovine oocytes |
title_full |
Vacuum-cooled liquid nitrogen increases the developmental ability of vitrified-warmed bovine oocytes |
title_fullStr |
Vacuum-cooled liquid nitrogen increases the developmental ability of vitrified-warmed bovine oocytes |
title_full_unstemmed |
Vacuum-cooled liquid nitrogen increases the developmental ability of vitrified-warmed bovine oocytes |
title_sort |
Vacuum-cooled liquid nitrogen increases the developmental ability of vitrified-warmed bovine oocytes |
author |
Santos,Rodrigo Marques dos |
author_facet |
Santos,Rodrigo Marques dos Barreta,Marcos Henrique Frajblat,Marcel Cucco,Diego Córdova Mezzalira,Joana Claudia Bunn,Silvério Cruz,Fabiano Buss Vieira,Arnaldo Diniz Mezzalira,Alceu |
author_role |
author |
author2 |
Barreta,Marcos Henrique Frajblat,Marcel Cucco,Diego Córdova Mezzalira,Joana Claudia Bunn,Silvério Cruz,Fabiano Buss Vieira,Arnaldo Diniz Mezzalira,Alceu |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Santos,Rodrigo Marques dos Barreta,Marcos Henrique Frajblat,Marcel Cucco,Diego Córdova Mezzalira,Joana Claudia Bunn,Silvério Cruz,Fabiano Buss Vieira,Arnaldo Diniz Mezzalira,Alceu |
dc.subject.por.fl_str_mv |
vitrification cryopreservation oocytes bovine vacuum |
topic |
vitrification cryopreservation oocytes bovine vacuum |
description |
The objective of this study was to determine the effects of vacuum-cooled liquid nitrogen on the development of vitrified immature (germinal vesicle stage; GV) and mature (metaphase II; MII) bovine oocytes after re-warming. Liquid nitrogen was exposed to either atmospheric pressure or to a vacuum (300mm Hg for 45sec); the latter decreased the temperature of the liquid nitrogen to -200°C. Partially denuded oocytes were vitrified either just after selection (GV) or after 22 hours of in vitro maturation (MII) in TCM 199 medium + 10% of estrous mare serum. For vitrification, oocytes were firstly exposed to an intermediate solution (10% EG + 10% DMSO) for 30sec, followed by the vitrification solution (20% EG + 20% DMSO + 0.5M sucrose) for 20sec. Groups of three or four oocytes were loaded into an open-pulled-straw and directly plunged into liquid nitrogen. Oocytes were subsequently re-warmed by exposure to air (25°C) for 4sec, followed by 5 min exposure to decreasing concentrations (0.3 and 0.15M) of sucrose. Fertilization (Day 0) was done with 2 x 106 spermatozoa mL-1 (selected by a swim-up procedure) and incubated for 18 to 22 hours. Presumptive zygotes were cultured at 39°C in four-well dishes with SOFaaci medium, under 5% CO2 and saturated humidity. Cleavage (Day 2) and blastocyst rates (Day 8) were 33.9 and 4.2%, respectively, for GV stage oocytes at atmospheric pressure, 41.2 and 8.8% for GV oocytes under vacuum, 43.5 and 6.7% for MII oocytes at atmospheric pressure, and 53.6 and 10.6% for MII oocytes under vacuum. In conclusion, vacuum-cooled liquid nitrogen improved developmental rates of vitrified-thawed bovine oocytes. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000500024 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000500024 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-84782006000500024 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.36 n.5 2006 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140527990702080 |