Isolation and characterization of canine parvovirus type 2c circulating in Uruguay

Detalhes bibliográficos
Autor(a) principal: Pintos,Andrea Blanc
Data de Publicação: 2011
Outros Autores: Larrama,Cecilia Beatriz Negro, Baratta,Eduardo Enrique Reolon, Barthe,Mabel Beatriz Berois, Rodonz,Juan Ramón Arbiza
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência Rural
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000800022
Resumo: This research reports the first CPV-2c isolation in cell culture (canine fibroma cell line A-72) in Uruguay. The isolates were obtained from 13 rectal swabs of Uruguayan dogs with parvovirosis. Samples were submitted to PCR with two sets of primers, restriction fragment length polymorphism (RFLP), partial sequencing of the gene encoding for VP2 capsid protein and phylogenetic characterization. The strain isolated was confirmed as CPV-2c. These results contribute to a better knowledge of CPV strains circulating in Uruguay and promote an evaluation of the efficacy of heterologous vaccines used to protect against the circulating strains.
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spelling Isolation and characterization of canine parvovirus type 2c circulating in Uruguaycanine parvovirosisCPV-2cvaccinated dogsThis research reports the first CPV-2c isolation in cell culture (canine fibroma cell line A-72) in Uruguay. The isolates were obtained from 13 rectal swabs of Uruguayan dogs with parvovirosis. Samples were submitted to PCR with two sets of primers, restriction fragment length polymorphism (RFLP), partial sequencing of the gene encoding for VP2 capsid protein and phylogenetic characterization. The strain isolated was confirmed as CPV-2c. These results contribute to a better knowledge of CPV strains circulating in Uruguay and promote an evaluation of the efficacy of heterologous vaccines used to protect against the circulating strains.Universidade Federal de Santa Maria2011-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000800022Ciência Rural v.41 n.8 2011reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/S0103-84782011005000098info:eu-repo/semantics/openAccessPintos,Andrea BlancLarrama,Cecilia Beatriz NegroBaratta,Eduardo Enrique ReolonBarthe,Mabel Beatriz BeroisRodonz,Juan Ramón Arbizaeng2011-08-18T00:00:00ZRevista
dc.title.none.fl_str_mv Isolation and characterization of canine parvovirus type 2c circulating in Uruguay
title Isolation and characterization of canine parvovirus type 2c circulating in Uruguay
spellingShingle Isolation and characterization of canine parvovirus type 2c circulating in Uruguay
Pintos,Andrea Blanc
canine parvovirosis
CPV-2c
vaccinated dogs
title_short Isolation and characterization of canine parvovirus type 2c circulating in Uruguay
title_full Isolation and characterization of canine parvovirus type 2c circulating in Uruguay
title_fullStr Isolation and characterization of canine parvovirus type 2c circulating in Uruguay
title_full_unstemmed Isolation and characterization of canine parvovirus type 2c circulating in Uruguay
title_sort Isolation and characterization of canine parvovirus type 2c circulating in Uruguay
author Pintos,Andrea Blanc
author_facet Pintos,Andrea Blanc
Larrama,Cecilia Beatriz Negro
Baratta,Eduardo Enrique Reolon
Barthe,Mabel Beatriz Berois
Rodonz,Juan Ramón Arbiza
author_role author
author2 Larrama,Cecilia Beatriz Negro
Baratta,Eduardo Enrique Reolon
Barthe,Mabel Beatriz Berois
Rodonz,Juan Ramón Arbiza
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Pintos,Andrea Blanc
Larrama,Cecilia Beatriz Negro
Baratta,Eduardo Enrique Reolon
Barthe,Mabel Beatriz Berois
Rodonz,Juan Ramón Arbiza
dc.subject.por.fl_str_mv canine parvovirosis
CPV-2c
vaccinated dogs
topic canine parvovirosis
CPV-2c
vaccinated dogs
description This research reports the first CPV-2c isolation in cell culture (canine fibroma cell line A-72) in Uruguay. The isolates were obtained from 13 rectal swabs of Uruguayan dogs with parvovirosis. Samples were submitted to PCR with two sets of primers, restriction fragment length polymorphism (RFLP), partial sequencing of the gene encoding for VP2 capsid protein and phylogenetic characterization. The strain isolated was confirmed as CPV-2c. These results contribute to a better knowledge of CPV strains circulating in Uruguay and promote an evaluation of the efficacy of heterologous vaccines used to protect against the circulating strains.
publishDate 2011
dc.date.none.fl_str_mv 2011-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000800022
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000800022
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-84782011005000098
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.41 n.8 2011
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
repository.name.fl_str_mv
repository.mail.fl_str_mv
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