Vegetative compatibility and molecular characterization of Fusarium graminearum isolates from the State of Paraná, Brazil

Detalhes bibliográficos
Autor(a) principal: Busso,Cleverson
Data de Publicação: 2007
Outros Autores: Kaneshima,Edilson Nobuyoshi, Franco,Francisco de Assis, Querol,Carmen Boto, Castro-Prado,Marialba Avezum Alves de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência Rural
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000600050
Resumo: Fusarium graminearum isolates causing Fusarium head blight in wheat were collected in Brazil and analyzed by random amplified polymorphic DNA (RAPD) markers and vegetative compatibility grouping (VCG). Nitrate non-utilizing mutants (nit) from each isolate were paired to verify heterokaryon formation. Three VCGs were identified among F. graminearum isolates: VCG1 included F-2, F-3 and F-4 isolates; VCG2 included F-1, F-6 and F-9 isolates; VCG3 included F-5, F-7 and F-8 isolates. Based on PCR amplification with eight different primers, the isolates showed great genetic similarity among themselves. Dendrogram analysis demonstrated two RAPD groups: Group A, consisting of isolates F-2 and F-9, and Group B, composed of the remaining isolates. Results suggest the clonal origin of F. graminearum isolates.
id UFSM-2_fa8e0fbb2a664f214c88dec6530d6e7c
oai_identifier_str oai:scielo:S0103-84782007000600050
network_acronym_str UFSM-2
network_name_str Ciência rural (Online)
repository_id_str
spelling Vegetative compatibility and molecular characterization of Fusarium graminearum isolates from the State of Paraná, BrazilFusarium graminearumgenetic variabilityRAPDvegetative compatibility groupsFusarium graminearum isolates causing Fusarium head blight in wheat were collected in Brazil and analyzed by random amplified polymorphic DNA (RAPD) markers and vegetative compatibility grouping (VCG). Nitrate non-utilizing mutants (nit) from each isolate were paired to verify heterokaryon formation. Three VCGs were identified among F. graminearum isolates: VCG1 included F-2, F-3 and F-4 isolates; VCG2 included F-1, F-6 and F-9 isolates; VCG3 included F-5, F-7 and F-8 isolates. Based on PCR amplification with eight different primers, the isolates showed great genetic similarity among themselves. Dendrogram analysis demonstrated two RAPD groups: Group A, consisting of isolates F-2 and F-9, and Group B, composed of the remaining isolates. Results suggest the clonal origin of F. graminearum isolates.Universidade Federal de Santa Maria2007-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000600050Ciência Rural v.37 n.6 2007reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/S0103-84782007000600050info:eu-repo/semantics/openAccessBusso,CleversonKaneshima,Edilson NobuyoshiFranco,Francisco de AssisQuerol,Carmen BotoCastro-Prado,Marialba Avezum Alves deeng2007-10-05T00:00:00ZRevista
dc.title.none.fl_str_mv Vegetative compatibility and molecular characterization of Fusarium graminearum isolates from the State of Paraná, Brazil
title Vegetative compatibility and molecular characterization of Fusarium graminearum isolates from the State of Paraná, Brazil
spellingShingle Vegetative compatibility and molecular characterization of Fusarium graminearum isolates from the State of Paraná, Brazil
Busso,Cleverson
Fusarium graminearum
genetic variability
RAPD
vegetative compatibility groups
title_short Vegetative compatibility and molecular characterization of Fusarium graminearum isolates from the State of Paraná, Brazil
title_full Vegetative compatibility and molecular characterization of Fusarium graminearum isolates from the State of Paraná, Brazil
title_fullStr Vegetative compatibility and molecular characterization of Fusarium graminearum isolates from the State of Paraná, Brazil
title_full_unstemmed Vegetative compatibility and molecular characterization of Fusarium graminearum isolates from the State of Paraná, Brazil
title_sort Vegetative compatibility and molecular characterization of Fusarium graminearum isolates from the State of Paraná, Brazil
author Busso,Cleverson
author_facet Busso,Cleverson
Kaneshima,Edilson Nobuyoshi
Franco,Francisco de Assis
Querol,Carmen Boto
Castro-Prado,Marialba Avezum Alves de
author_role author
author2 Kaneshima,Edilson Nobuyoshi
Franco,Francisco de Assis
Querol,Carmen Boto
Castro-Prado,Marialba Avezum Alves de
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Busso,Cleverson
Kaneshima,Edilson Nobuyoshi
Franco,Francisco de Assis
Querol,Carmen Boto
Castro-Prado,Marialba Avezum Alves de
dc.subject.por.fl_str_mv Fusarium graminearum
genetic variability
RAPD
vegetative compatibility groups
topic Fusarium graminearum
genetic variability
RAPD
vegetative compatibility groups
description Fusarium graminearum isolates causing Fusarium head blight in wheat were collected in Brazil and analyzed by random amplified polymorphic DNA (RAPD) markers and vegetative compatibility grouping (VCG). Nitrate non-utilizing mutants (nit) from each isolate were paired to verify heterokaryon formation. Three VCGs were identified among F. graminearum isolates: VCG1 included F-2, F-3 and F-4 isolates; VCG2 included F-1, F-6 and F-9 isolates; VCG3 included F-5, F-7 and F-8 isolates. Based on PCR amplification with eight different primers, the isolates showed great genetic similarity among themselves. Dendrogram analysis demonstrated two RAPD groups: Group A, consisting of isolates F-2 and F-9, and Group B, composed of the remaining isolates. Results suggest the clonal origin of F. graminearum isolates.
publishDate 2007
dc.date.none.fl_str_mv 2007-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000600050
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000600050
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-84782007000600050
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.37 n.6 2007
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1749140529730289664

Registros relacionados