ESTABLISHMENT OF ASEPTIC CULTURES OF Aspidosperma polyneuron

• Autor(a) principal: Ribas, Luciana Lopes Fortes
• Data de Publicação: 2005
• Outros Autores: Zanette, Flávio, Kulchetscki, Luiz, Guerra, Miguel Pedro
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Ciência Florestal (Online)
• Texto Completo: https://periodicos.ufsm.br/cienciaflorestal/article/view/1729
• topic: peroba; micropropagation; juvenile explant; disinfection.; peroba-rosa; micropropagação; explante juvenil; desinfestação

The objective of the present work was to obtain aseptic cultures in order to establish a micropropagation protocol of Aspidosperma polyneuron from juvenile explants. Apical shoots from two-year--old seedlings were collected in a greenhouse and disinfected with sodium hypochlorite (0.125 or 0.25%) or mercuric chloride (0.025, 0.05 or 0.1%), during 5 or 10 minutes and at different seasons of the year. Necrosis, bacterial and fungal contamination percentages and survival rates were evaluated after three weeks. It was showed that NaOCl 0.25% solution, during 10 minutes resulted in 70% of survival and disinfection of apical shoots, independent of the season of the year (summer or autumn). HgCl2 was more efficient than NaOCl in the disinfection of apical shoots, therefore 0.05% HgCl2, during 10 minutes was recommended (84.10% of survival). Aseptic cultures were established in all seasons of the year, but better results were obtained in spring and summer.