IN VITRO PRODUCTIVITY OF APULEIA (Apuleia leiocarpa) MICROSTUMPS

Detalhes bibliográficos
Autor(a) principal: Lencina, Kelen Haygert
Data de Publicação: 2018
Outros Autores: Bisognin, Dilson Antônio, Pimentel, Nathalia, Kielse, Paula, Mello, Uilian Stefanello de
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência Florestal (Online)
Texto Completo: https://periodicos.ufsm.br/cienciaflorestal/article/view/31635
Resumo: The aim of this work was to evaluate the in vitro productivity of grapia (Apuleia leiocarpa) micro-stumps cultivated in different media conditions to maximize its multiplication rate. Micro-stumps from aseptic seedlings were cultivated in WPM medium added with 0, 2.2, 4.4, 6.6 and 8.8 µM of 6-benzylaminopurine (BAP) and subcultured in WPM medium without cytokinin. Micro-stumps were also cultivated in WPM, MS or ½ MS base media with or without the addition of 1.5 g L-1 of activated charcoal and submitted to drastic pruning at 30, 60 and 90 days of cultivation. The in vitro cultivation of grapia micro-stumps in WPM or ½ MS media added with 8.8 µM of BAP and the subcultivation in media without cytokinin increase the number and length of shoots. The cultivation of grapia micro-stumps in media with activated charcoal increases the productivity of micro-cuttings per micro-stumps, but in the absence of activated charcoal induces callus formation and indirect organogenesis of shoots. The in vitro conservation of grapia micro-stumps maximizes the multiplication rate, being a feasible technique for the production of grapia plantlets.
id UFSM-6_4d3d374c466ef811391ba57f44f7b639
oai_identifier_str oai:ojs.pkp.sfu.ca:article/31635
network_acronym_str UFSM-6
network_name_str Ciência Florestal (Online)
repository_id_str
spelling IN VITRO PRODUCTIVITY OF APULEIA (Apuleia leiocarpa) MICROSTUMPSPRODUTIVIDADE DE MICROCEPAS DE GRÁPIA (Apuleia leiocarpa) MANTIDAS IN VITROmicropropagationin vitro propagationvegetative propagation.microprogaçãopropagação in vitropropagação vegetativa.The aim of this work was to evaluate the in vitro productivity of grapia (Apuleia leiocarpa) micro-stumps cultivated in different media conditions to maximize its multiplication rate. Micro-stumps from aseptic seedlings were cultivated in WPM medium added with 0, 2.2, 4.4, 6.6 and 8.8 µM of 6-benzylaminopurine (BAP) and subcultured in WPM medium without cytokinin. Micro-stumps were also cultivated in WPM, MS or ½ MS base media with or without the addition of 1.5 g L-1 of activated charcoal and submitted to drastic pruning at 30, 60 and 90 days of cultivation. The in vitro cultivation of grapia micro-stumps in WPM or ½ MS media added with 8.8 µM of BAP and the subcultivation in media without cytokinin increase the number and length of shoots. The cultivation of grapia micro-stumps in media with activated charcoal increases the productivity of micro-cuttings per micro-stumps, but in the absence of activated charcoal induces callus formation and indirect organogenesis of shoots. The in vitro conservation of grapia micro-stumps maximizes the multiplication rate, being a feasible technique for the production of grapia plantlets.O objetivo deste trabalho foi avaliar a produtividade de microcepas de grápia (Apuleia leiocarpa) mantidas em diferentes condições de cultivo in vitro, visando maximizar a taxa de multiplicação. Microcepas oriundas da poda drástica de plantas assépticas foram cultivadas em meio de cultura WPM acrescida de 0; 2,2; 4,4; 6,6 e 8,8 µM de 6-benzilaminopurina (BAP) e subcultivadas em meio de cultura WPM sem citocinina. Microcepas de grápia também foram cultivadas em meio de cultura WPM, MS ou ½ MS, acrescido ou não de 1,5 g L-1 de carvão ativado e submetidas a três coletas de brotos aos 30, 60 e 90 dias de cultivo. A manutenção das microcepas em meio de cultura WPM ou ½ MS suplementado com 8,8 µM de BAP, seguido do subcultivo em meio de cultura sem citocinina aumenta o número e o crescimento de brotações. O cultivo de microcepas de grápia em meio de cultura com carvão ativado aumenta a produção de microestacas por microcepas, enquanto a ausência de carvão ativado favorece a formação de calos e brotos por organogênese indireta. A manutenção in vitro de microcepas de grápia maximiza a taxa de multiplicação, constituindo-se de uma técnica promissora para a produção de plantas de grápia.Universidade Federal de Santa Maria2018-04-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsm.br/cienciaflorestal/article/view/3163510.5902/1980509831635Ciência Florestal; Vol. 28 No. 1 (2018); 150-159Ciência Florestal; v. 28 n. 1 (2018); 150-1591980-50980103-9954reponame:Ciência Florestal (Online)instname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMporhttps://periodicos.ufsm.br/cienciaflorestal/article/view/31635/pdfCopyright (c) 2018 Ciência Florestalinfo:eu-repo/semantics/openAccessLencina, Kelen HaygertBisognin, Dilson AntônioPimentel, NathaliaKielse, PaulaMello, Uilian Stefanello de2018-04-23T18:12:41Zoai:ojs.pkp.sfu.ca:article/31635Revistahttp://www.ufsm.br/cienciaflorestal/ONGhttps://old.scielo.br/oai/scielo-oai.php||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br1980-50980103-9954opendoar:2018-04-23T18:12:41Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv IN VITRO PRODUCTIVITY OF APULEIA (Apuleia leiocarpa) MICROSTUMPS
PRODUTIVIDADE DE MICROCEPAS DE GRÁPIA (Apuleia leiocarpa) MANTIDAS IN VITRO
title IN VITRO PRODUCTIVITY OF APULEIA (Apuleia leiocarpa) MICROSTUMPS
spellingShingle IN VITRO PRODUCTIVITY OF APULEIA (Apuleia leiocarpa) MICROSTUMPS
Lencina, Kelen Haygert
micropropagation
in vitro propagation
vegetative propagation.
microprogação
propagação in vitro
propagação vegetativa.
title_short IN VITRO PRODUCTIVITY OF APULEIA (Apuleia leiocarpa) MICROSTUMPS
title_full IN VITRO PRODUCTIVITY OF APULEIA (Apuleia leiocarpa) MICROSTUMPS
title_fullStr IN VITRO PRODUCTIVITY OF APULEIA (Apuleia leiocarpa) MICROSTUMPS
title_full_unstemmed IN VITRO PRODUCTIVITY OF APULEIA (Apuleia leiocarpa) MICROSTUMPS
title_sort IN VITRO PRODUCTIVITY OF APULEIA (Apuleia leiocarpa) MICROSTUMPS
author Lencina, Kelen Haygert
author_facet Lencina, Kelen Haygert
Bisognin, Dilson Antônio
Pimentel, Nathalia
Kielse, Paula
Mello, Uilian Stefanello de
author_role author
author2 Bisognin, Dilson Antônio
Pimentel, Nathalia
Kielse, Paula
Mello, Uilian Stefanello de
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Lencina, Kelen Haygert
Bisognin, Dilson Antônio
Pimentel, Nathalia
Kielse, Paula
Mello, Uilian Stefanello de
dc.subject.por.fl_str_mv micropropagation
in vitro propagation
vegetative propagation.
microprogação
propagação in vitro
propagação vegetativa.
topic micropropagation
in vitro propagation
vegetative propagation.
microprogação
propagação in vitro
propagação vegetativa.
description The aim of this work was to evaluate the in vitro productivity of grapia (Apuleia leiocarpa) micro-stumps cultivated in different media conditions to maximize its multiplication rate. Micro-stumps from aseptic seedlings were cultivated in WPM medium added with 0, 2.2, 4.4, 6.6 and 8.8 µM of 6-benzylaminopurine (BAP) and subcultured in WPM medium without cytokinin. Micro-stumps were also cultivated in WPM, MS or ½ MS base media with or without the addition of 1.5 g L-1 of activated charcoal and submitted to drastic pruning at 30, 60 and 90 days of cultivation. The in vitro cultivation of grapia micro-stumps in WPM or ½ MS media added with 8.8 µM of BAP and the subcultivation in media without cytokinin increase the number and length of shoots. The cultivation of grapia micro-stumps in media with activated charcoal increases the productivity of micro-cuttings per micro-stumps, but in the absence of activated charcoal induces callus formation and indirect organogenesis of shoots. The in vitro conservation of grapia micro-stumps maximizes the multiplication rate, being a feasible technique for the production of grapia plantlets.
publishDate 2018
dc.date.none.fl_str_mv 2018-04-02
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.ufsm.br/cienciaflorestal/article/view/31635
10.5902/1980509831635
url https://periodicos.ufsm.br/cienciaflorestal/article/view/31635
identifier_str_mv 10.5902/1980509831635
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.ufsm.br/cienciaflorestal/article/view/31635/pdf
dc.rights.driver.fl_str_mv Copyright (c) 2018 Ciência Florestal
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Ciência Florestal
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Florestal; Vol. 28 No. 1 (2018); 150-159
Ciência Florestal; v. 28 n. 1 (2018); 150-159
1980-5098
0103-9954
reponame:Ciência Florestal (Online)
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Florestal (Online)
collection Ciência Florestal (Online)
repository.name.fl_str_mv Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv ||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br
_version_ 1789434750108172288

Registros relacionados