Viability of stored seeds of Himatanthus sucuuba Wood by tetrazolium test
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Ciência Florestal (Online) |
Texto Completo: | https://periodicos.ufsm.br/cienciaflorestal/article/view/42809 |
Resumo: | Himatanthus sucuuba is an Amazonian tree species of importance in traditional medicine because of its therapeutic properties. Estimates of seed viability are still scarce, so it is necessary to develop rapid tests to evaluate seed quality. The objectives of this work were to adapt the methodology for the tetrazolium test and to verify the viability of the stored seeds of Himatanthus sucuuba by comparing the results from the germination and tetrazolium test. For preconditioning, the seeds were immersed in distilled water for the periods of 4, 8, 12 and 14 hours at 25°C. Subsequently, seed tissue staining was performed by immersion in a tetrazolium solution of 0.025%, 0.075% and 0.100% for 90 minutes at 40°C in the dark conditions, and the seeds were evaluated into six classes. At the same time, germination tests were conducted in a BOD chamber at 30°C, establishing criteria for viability and vigor evaluation. The experimental design was completely randomized, and the comparison of means performed by Tukey test at 5% significance. The tetrazolium test at a concentration of 0.075% at 40°C for 90 minutes was efficient to evaluate the viability and vigor of Himatanthus sucuuba seeds. The comparison between germination (78%) and tetrazolium test (70%) showed no significant difference, concluding that seeds stored for 14 months remained viable |
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Viability of stored seeds of Himatanthus sucuuba Wood by tetrazolium testViabilidade de sementes armazenadas de Himatanthus sucuuba Wood pelo teste de tetrazólioSeed qualityMedicinal speciesGerminationQualidade de sementesEspécie medicinalGerminaçãoHimatanthus sucuuba is an Amazonian tree species of importance in traditional medicine because of its therapeutic properties. Estimates of seed viability are still scarce, so it is necessary to develop rapid tests to evaluate seed quality. The objectives of this work were to adapt the methodology for the tetrazolium test and to verify the viability of the stored seeds of Himatanthus sucuuba by comparing the results from the germination and tetrazolium test. For preconditioning, the seeds were immersed in distilled water for the periods of 4, 8, 12 and 14 hours at 25°C. Subsequently, seed tissue staining was performed by immersion in a tetrazolium solution of 0.025%, 0.075% and 0.100% for 90 minutes at 40°C in the dark conditions, and the seeds were evaluated into six classes. At the same time, germination tests were conducted in a BOD chamber at 30°C, establishing criteria for viability and vigor evaluation. The experimental design was completely randomized, and the comparison of means performed by Tukey test at 5% significance. The tetrazolium test at a concentration of 0.075% at 40°C for 90 minutes was efficient to evaluate the viability and vigor of Himatanthus sucuuba seeds. The comparison between germination (78%) and tetrazolium test (70%) showed no significant difference, concluding that seeds stored for 14 months remained viableHimatanthus sucuuba é uma espécie arbórea amazônica, de importância na medicina popular devido às suas propriedades terapêuticas. As estimativas da viabilidade de sementes dessa espécie ainda são escassas, tornando-se necessário o desenvolvimento de testes rápidos que permitam a avaliação da qualidade das sementes. O objetivo deste trabalho foi adequar a metodologia para a realização do teste de tetrazólio e verificar a viabilidade das sementes armazenadas de Himatanthus sucuuba comparando os resultados dos testes de germinação e tetrazólio. Para o precondicionamento, as sementes foram imersas em água destilada por períodos de 4, 8, 12 e 14 horas a 25°C. Posteriormente, foi realizada a coloração dos tecidos, pela imersão em solução de tetrazólio 0,025%, 0,075% e 0,100% por 90 minutos a 40°C no escuro, e para a avaliação as sementes foram distribuídas em seis classes. Paralelamente, foram conduzidos testes de germinação em câmara BOD a 30°C, estabelecendo-se critérios para avaliação da viabilidade e do vigor. O delineamento experimental foi o inteiramente casualizado, e a comparação de médias foi realizada pelo teste de Tukey a 5% de significância. O teste de tetrazólio na concentração de 0,075% a 40°C durante 90 minutos mostrou-se eficiente para avaliar a viabilidade e vigor das sementes de Himatanthus sucuuba. A comparação entre a germinação (78%) e o teste de tetrazólio (70%), não mostrou diferença significativa entre eles, concluindo-se que sementes armazenadas por 14 meses permaneceram viáveis.Universidade Federal de Santa Maria2021-03-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://periodicos.ufsm.br/cienciaflorestal/article/view/4280910.5902/1980509842809Ciência Florestal; Vol. 31 No. 1 (2021); 333-349Ciência Florestal; v. 31 n. 1 (2021); 333-3491980-50980103-9954reponame:Ciência Florestal (Online)instname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMporhttps://periodicos.ufsm.br/cienciaflorestal/article/view/42809/pdfhttps://periodicos.ufsm.br/cienciaflorestal/article/view/42809/htmlCopyright (c) 2021 Ciência Florestalinfo:eu-repo/semantics/openAccessSáenz-Ramírez, Lyanna HellenMendes, Angela Maria da SilvaImakawa, Angela MariaSampaio, Paulo de Tarso BarbosaMelo, Maria da Glória Gonçalves de2021-05-20T04:00:55Zoai:ojs.pkp.sfu.ca:article/42809Revistahttp://www.ufsm.br/cienciaflorestal/ONGhttps://old.scielo.br/oai/scielo-oai.php||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br1980-50980103-9954opendoar:2021-05-20T04:00:55Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Viability of stored seeds of Himatanthus sucuuba Wood by tetrazolium test Viabilidade de sementes armazenadas de Himatanthus sucuuba Wood pelo teste de tetrazólio |
title |
Viability of stored seeds of Himatanthus sucuuba Wood by tetrazolium test |
spellingShingle |
Viability of stored seeds of Himatanthus sucuuba Wood by tetrazolium test Sáenz-Ramírez, Lyanna Hellen Seed quality Medicinal species Germination Qualidade de sementes Espécie medicinal Germinação |
title_short |
Viability of stored seeds of Himatanthus sucuuba Wood by tetrazolium test |
title_full |
Viability of stored seeds of Himatanthus sucuuba Wood by tetrazolium test |
title_fullStr |
Viability of stored seeds of Himatanthus sucuuba Wood by tetrazolium test |
title_full_unstemmed |
Viability of stored seeds of Himatanthus sucuuba Wood by tetrazolium test |
title_sort |
Viability of stored seeds of Himatanthus sucuuba Wood by tetrazolium test |
author |
Sáenz-Ramírez, Lyanna Hellen |
author_facet |
Sáenz-Ramírez, Lyanna Hellen Mendes, Angela Maria da Silva Imakawa, Angela Maria Sampaio, Paulo de Tarso Barbosa Melo, Maria da Glória Gonçalves de |
author_role |
author |
author2 |
Mendes, Angela Maria da Silva Imakawa, Angela Maria Sampaio, Paulo de Tarso Barbosa Melo, Maria da Glória Gonçalves de |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Sáenz-Ramírez, Lyanna Hellen Mendes, Angela Maria da Silva Imakawa, Angela Maria Sampaio, Paulo de Tarso Barbosa Melo, Maria da Glória Gonçalves de |
dc.subject.por.fl_str_mv |
Seed quality Medicinal species Germination Qualidade de sementes Espécie medicinal Germinação |
topic |
Seed quality Medicinal species Germination Qualidade de sementes Espécie medicinal Germinação |
description |
Himatanthus sucuuba is an Amazonian tree species of importance in traditional medicine because of its therapeutic properties. Estimates of seed viability are still scarce, so it is necessary to develop rapid tests to evaluate seed quality. The objectives of this work were to adapt the methodology for the tetrazolium test and to verify the viability of the stored seeds of Himatanthus sucuuba by comparing the results from the germination and tetrazolium test. For preconditioning, the seeds were immersed in distilled water for the periods of 4, 8, 12 and 14 hours at 25°C. Subsequently, seed tissue staining was performed by immersion in a tetrazolium solution of 0.025%, 0.075% and 0.100% for 90 minutes at 40°C in the dark conditions, and the seeds were evaluated into six classes. At the same time, germination tests were conducted in a BOD chamber at 30°C, establishing criteria for viability and vigor evaluation. The experimental design was completely randomized, and the comparison of means performed by Tukey test at 5% significance. The tetrazolium test at a concentration of 0.075% at 40°C for 90 minutes was efficient to evaluate the viability and vigor of Himatanthus sucuuba seeds. The comparison between germination (78%) and tetrazolium test (70%) showed no significant difference, concluding that seeds stored for 14 months remained viable |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-03-15 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufsm.br/cienciaflorestal/article/view/42809 10.5902/1980509842809 |
url |
https://periodicos.ufsm.br/cienciaflorestal/article/view/42809 |
identifier_str_mv |
10.5902/1980509842809 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.ufsm.br/cienciaflorestal/article/view/42809/pdf https://periodicos.ufsm.br/cienciaflorestal/article/view/42809/html |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Ciência Florestal info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Ciência Florestal |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Florestal; Vol. 31 No. 1 (2021); 333-349 Ciência Florestal; v. 31 n. 1 (2021); 333-349 1980-5098 0103-9954 reponame:Ciência Florestal (Online) instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Florestal (Online) |
collection |
Ciência Florestal (Online) |
repository.name.fl_str_mv |
Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br |
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1789434751913820160 |