INDIRECT ORGANOGENESIS FROM LEAF EXPLANTS AND IN VITRO SHOOTS MULTIPLICATION OF Eucalyptus benthamii X Eucalyptus dunnii
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Ciência Florestal (Online) |
Texto Completo: | https://periodicos.ufsm.br/cienciaflorestal/article/view/14572 |
Resumo: | http://dx.doi.org/10.5902/1980509814572The aims of this research were to evaluate different culture media for indirect organogenesis and shoot multiplication of Eucalyptus benthamii x Eucalyptus dunnii. For organogenesis, leaf explants were used to test the following treatments: two culture media (MS N/2 and JADS) supplemented with 0.1 μM 1-naphthaleneacetic acid (NAA) and thidiazuron (TDZ) (0.1 or 0.5 μM), with or without PVP- 40 (250 mg L-1). The percentage of oxidized explants, callus forming explants, explants with anthocyanin, buds, shoots and the shoot number per explant were evaluated. In the multiplication experiment, isolated shoots were cultivated in MS, JADS and WPM media, all supplemented with 1.11 μM BAP. Four subcultures were carried out every 28 days. In every subculture the explant oxidation, partial or total leaf chlorosis, fresh mass and mean number of shoot per explant were evaluated. The MS N/2 medium supplemented with 0.1 μM NAA and 0.5 μM TDZ promoted the highest rate of organogenesis (8.3%) and the culture media MS supplemented with 1.11 μM BAP the multiplication rate was higher than in the other media, in the first and the second subcultures (9.28 and 9.24, respectively), without differences between the three media in the following subcultures. |
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INDIRECT ORGANOGENESIS FROM LEAF EXPLANTS AND IN VITRO SHOOTS MULTIPLICATION OF Eucalyptus benthamii X Eucalyptus dunniiOrganogênese indireta a partir de explantes foliares e multiplicação in vitro de brotações de Eucalyptus benthamii X Eucalyptus dunniiregenerationwoody speciesplant growth regulatorsculture mediumregeneraçãoespécie lenhosaregulador vegetalmeio de cultura.http://dx.doi.org/10.5902/1980509814572The aims of this research were to evaluate different culture media for indirect organogenesis and shoot multiplication of Eucalyptus benthamii x Eucalyptus dunnii. For organogenesis, leaf explants were used to test the following treatments: two culture media (MS N/2 and JADS) supplemented with 0.1 μM 1-naphthaleneacetic acid (NAA) and thidiazuron (TDZ) (0.1 or 0.5 μM), with or without PVP- 40 (250 mg L-1). The percentage of oxidized explants, callus forming explants, explants with anthocyanin, buds, shoots and the shoot number per explant were evaluated. In the multiplication experiment, isolated shoots were cultivated in MS, JADS and WPM media, all supplemented with 1.11 μM BAP. Four subcultures were carried out every 28 days. In every subculture the explant oxidation, partial or total leaf chlorosis, fresh mass and mean number of shoot per explant were evaluated. The MS N/2 medium supplemented with 0.1 μM NAA and 0.5 μM TDZ promoted the highest rate of organogenesis (8.3%) and the culture media MS supplemented with 1.11 μM BAP the multiplication rate was higher than in the other media, in the first and the second subcultures (9.28 and 9.24, respectively), without differences between the three media in the following subcultures. http://dx.doi.org/10.5902/1980509814572Os objetivos deste trabalho foram avaliar diferentes meios de cultura na organogênese indireta e na multiplicação in vitro de brotos de Eucalyptus benthamii x Eucalyptus dunnii. Para organogênese, explantes foliares foram excisados no sentido transversal e cultivados in vitro, sendo os seguintes fatores testados: dois meios de cultura (MS N/2 e JADS) adicionados de 0,1 μM de ANA, duas concentrações de thidiazuron (0,1 e 0,5 μM) e presença ou não de PVP-40 (250 mg L-1). Após 70 dias de cultivo foram avaliadas as porcentagens de explantes oxidados totalmente, formando calo, produzindo antocianina, formando gema, formando brotações e o número de brotações formadas por explante regenerando. No experimento de multiplicação, brotações isoladas foram cultivadas em meio MS, JADS e WPM, adicionados de 1,11 μM de BAP. Foram realizados quatro subcultivos a cada 28 dias e em cada subcultivo foram avaliados: a porcentagem de oxidação, de explantes apresentando clorose total ou parcial, massa fresca e número médio de brotos por explante. O meio de cultura MS N/2 suplementado com 0,1 μM de ANA, 0,5 μM de TDZ e PVP-40 promoveu a maior taxa de organogênese (8,3%). No meio de cultura MS com 1,11 μM de BAP, a taxa de multiplicação foi maior que nos outros meios, no primeiro e segundo subcultivos (9,28 e 9,24 por mês), não havendo diferença entre os três meios nos demais subcultivos.Universidade Federal de Santa Maria2014-06-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsm.br/cienciaflorestal/article/view/1457210.5902/1980509814572Ciência Florestal; Vol. 24 No. 2 (2014); 347-355Ciência Florestal; v. 24 n. 2 (2014); 347-3551980-50980103-9954reponame:Ciência Florestal (Online)instname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMporhttps://periodicos.ufsm.br/cienciaflorestal/article/view/14572/pdf_1Oliveira-Cauduro, Yohana deAdamuchio, Laís GomesDegenhardt-Goldbach, JulianaBespalhok Filho, João CarlosDibax, RobersonQuoirin, Margueriteinfo:eu-repo/semantics/openAccess2017-04-13T12:07:19Zoai:ojs.pkp.sfu.ca:article/14572Revistahttp://www.ufsm.br/cienciaflorestal/ONGhttps://old.scielo.br/oai/scielo-oai.php||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br1980-50980103-9954opendoar:2017-04-13T12:07:19Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
INDIRECT ORGANOGENESIS FROM LEAF EXPLANTS AND IN VITRO SHOOTS MULTIPLICATION OF Eucalyptus benthamii X Eucalyptus dunnii Organogênese indireta a partir de explantes foliares e multiplicação in vitro de brotações de Eucalyptus benthamii X Eucalyptus dunnii |
title |
INDIRECT ORGANOGENESIS FROM LEAF EXPLANTS AND IN VITRO SHOOTS MULTIPLICATION OF Eucalyptus benthamii X Eucalyptus dunnii |
spellingShingle |
INDIRECT ORGANOGENESIS FROM LEAF EXPLANTS AND IN VITRO SHOOTS MULTIPLICATION OF Eucalyptus benthamii X Eucalyptus dunnii Oliveira-Cauduro, Yohana de regeneration woody species plant growth regulators culture medium regeneração espécie lenhosa regulador vegetal meio de cultura. |
title_short |
INDIRECT ORGANOGENESIS FROM LEAF EXPLANTS AND IN VITRO SHOOTS MULTIPLICATION OF Eucalyptus benthamii X Eucalyptus dunnii |
title_full |
INDIRECT ORGANOGENESIS FROM LEAF EXPLANTS AND IN VITRO SHOOTS MULTIPLICATION OF Eucalyptus benthamii X Eucalyptus dunnii |
title_fullStr |
INDIRECT ORGANOGENESIS FROM LEAF EXPLANTS AND IN VITRO SHOOTS MULTIPLICATION OF Eucalyptus benthamii X Eucalyptus dunnii |
title_full_unstemmed |
INDIRECT ORGANOGENESIS FROM LEAF EXPLANTS AND IN VITRO SHOOTS MULTIPLICATION OF Eucalyptus benthamii X Eucalyptus dunnii |
title_sort |
INDIRECT ORGANOGENESIS FROM LEAF EXPLANTS AND IN VITRO SHOOTS MULTIPLICATION OF Eucalyptus benthamii X Eucalyptus dunnii |
author |
Oliveira-Cauduro, Yohana de |
author_facet |
Oliveira-Cauduro, Yohana de Adamuchio, Laís Gomes Degenhardt-Goldbach, Juliana Bespalhok Filho, João Carlos Dibax, Roberson Quoirin, Marguerite |
author_role |
author |
author2 |
Adamuchio, Laís Gomes Degenhardt-Goldbach, Juliana Bespalhok Filho, João Carlos Dibax, Roberson Quoirin, Marguerite |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira-Cauduro, Yohana de Adamuchio, Laís Gomes Degenhardt-Goldbach, Juliana Bespalhok Filho, João Carlos Dibax, Roberson Quoirin, Marguerite |
dc.subject.por.fl_str_mv |
regeneration woody species plant growth regulators culture medium regeneração espécie lenhosa regulador vegetal meio de cultura. |
topic |
regeneration woody species plant growth regulators culture medium regeneração espécie lenhosa regulador vegetal meio de cultura. |
description |
http://dx.doi.org/10.5902/1980509814572The aims of this research were to evaluate different culture media for indirect organogenesis and shoot multiplication of Eucalyptus benthamii x Eucalyptus dunnii. For organogenesis, leaf explants were used to test the following treatments: two culture media (MS N/2 and JADS) supplemented with 0.1 μM 1-naphthaleneacetic acid (NAA) and thidiazuron (TDZ) (0.1 or 0.5 μM), with or without PVP- 40 (250 mg L-1). The percentage of oxidized explants, callus forming explants, explants with anthocyanin, buds, shoots and the shoot number per explant were evaluated. In the multiplication experiment, isolated shoots were cultivated in MS, JADS and WPM media, all supplemented with 1.11 μM BAP. Four subcultures were carried out every 28 days. In every subculture the explant oxidation, partial or total leaf chlorosis, fresh mass and mean number of shoot per explant were evaluated. The MS N/2 medium supplemented with 0.1 μM NAA and 0.5 μM TDZ promoted the highest rate of organogenesis (8.3%) and the culture media MS supplemented with 1.11 μM BAP the multiplication rate was higher than in the other media, in the first and the second subcultures (9.28 and 9.24, respectively), without differences between the three media in the following subcultures. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-06-27 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufsm.br/cienciaflorestal/article/view/14572 10.5902/1980509814572 |
url |
https://periodicos.ufsm.br/cienciaflorestal/article/view/14572 |
identifier_str_mv |
10.5902/1980509814572 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.ufsm.br/cienciaflorestal/article/view/14572/pdf_1 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Florestal; Vol. 24 No. 2 (2014); 347-355 Ciência Florestal; v. 24 n. 2 (2014); 347-355 1980-5098 0103-9954 reponame:Ciência Florestal (Online) instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Florestal (Online) |
collection |
Ciência Florestal (Online) |
repository.name.fl_str_mv |
Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br |
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1799944129231192064 |