Líquido folicular eqüino na produção in vitro de embriões bovinos
Autor(a) principal: | |
---|---|
Data de Publicação: | 2002 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações do UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/26712 |
Resumo: | Follicular fluid has been used for in vitro embryo production, although it offers the risk of disease transmission. The use of heterologous follicular fluid can be an alternative to avoid infection. The effect of equine follicular fluid (eFF) on embryonic development of bovine oocytes was evaluated. In Experiment I (EI), eFF was used as holding medium for immature bovine oocytes. In Experiment II (EII), maturation medium was supplemented with different concentrations of eFF. Oocytes for both experiments were obtained from ovaries collected at abattoir. For Experimennt I, 1228 Cumulus-oocyte complexes (COC´s) were distributed (13 replicate) on 3 treatments: T1, T2 and T3 and a control group (C). COC´s of the Control Group and T1 were matured (IVM) for 24 and 18h, respectively. COC´s of T2 and T3 were exposed, respectively, to eFF and bovine follicular fluid (bFF) for 6h at 30ºC, and matured for 18h. TCM 199 was used for IVM. On EII, 1045 COC`s were distributed (11 replicate) on 3 treatments: T1, T2 e T3 and a control group. TCM-199 with sodium pyruvate, rFSHh and Lhe was used as maturation medium. This basic medium was supplemented with 10% estrous mare serum for the control group, and 5, 10, 20% of eLF to treatments T1, T2 e T3, respectively. The COC´s were in vitro matured (IVM) for 24 hours. In vitro fertilization (IVF) and culture offers the same conditions for both experiments. IVF was performed in TALP-FERT medium. IVM and IVF were carried out in an incubator at 39ºC with 5% CO2 in air and saturated humidity. Zygotes were cultured in SOFaaci medium, under mineral oil in gasified plastic bags (Bag System). In experiment I, cleavage rate was lower in COC´s matured for 18h (T1, T2 and T3) than for 24h (Control). No differences were observed on blastocyst rates on D7 and hatching rates on D9. When oocytes were matured for 18h, blastocyst rates on D9 were higher on those COC´s exposed to Follicular Fluid. In experiment II, no differences were observed on cleavage and hatching rates. Blastocyst production on D7 and D9 were higher in T2 than T1 (P<0,05). Equine follicular fluid can be used for holding and maturing bovine oocytes producing similar rates of in vitro embryo development than that obtained for control group. |
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2022-10-31T18:04:33Z2022-10-31T18:04:33Z2002-01-07http://repositorio.ufsm.br/handle/1/26712Follicular fluid has been used for in vitro embryo production, although it offers the risk of disease transmission. The use of heterologous follicular fluid can be an alternative to avoid infection. The effect of equine follicular fluid (eFF) on embryonic development of bovine oocytes was evaluated. In Experiment I (EI), eFF was used as holding medium for immature bovine oocytes. In Experiment II (EII), maturation medium was supplemented with different concentrations of eFF. Oocytes for both experiments were obtained from ovaries collected at abattoir. For Experimennt I, 1228 Cumulus-oocyte complexes (COC´s) were distributed (13 replicate) on 3 treatments: T1, T2 and T3 and a control group (C). COC´s of the Control Group and T1 were matured (IVM) for 24 and 18h, respectively. COC´s of T2 and T3 were exposed, respectively, to eFF and bovine follicular fluid (bFF) for 6h at 30ºC, and matured for 18h. TCM 199 was used for IVM. On EII, 1045 COC`s were distributed (11 replicate) on 3 treatments: T1, T2 e T3 and a control group. TCM-199 with sodium pyruvate, rFSHh and Lhe was used as maturation medium. This basic medium was supplemented with 10% estrous mare serum for the control group, and 5, 10, 20% of eLF to treatments T1, T2 e T3, respectively. The COC´s were in vitro matured (IVM) for 24 hours. In vitro fertilization (IVF) and culture offers the same conditions for both experiments. IVF was performed in TALP-FERT medium. IVM and IVF were carried out in an incubator at 39ºC with 5% CO2 in air and saturated humidity. Zygotes were cultured in SOFaaci medium, under mineral oil in gasified plastic bags (Bag System). In experiment I, cleavage rate was lower in COC´s matured for 18h (T1, T2 and T3) than for 24h (Control). No differences were observed on blastocyst rates on D7 and hatching rates on D9. When oocytes were matured for 18h, blastocyst rates on D9 were higher on those COC´s exposed to Follicular Fluid. In experiment II, no differences were observed on cleavage and hatching rates. Blastocyst production on D7 and D9 were higher in T2 than T1 (P<0,05). Equine follicular fluid can be used for holding and maturing bovine oocytes producing similar rates of in vitro embryo development than that obtained for control group.O líquido folicular tem sido empregado na produção in vitro de embriões, embora ofereça o risco de transmissão de doenças. A utilização de líquido folicular heterólogo pode ser uma alternativa para se evitar a infecção por agentes específicos. Desse modo, foram avaliados os efeitos do Líquido Folicular Eqüino (LFe) sobre o desenvolvimento embrionário de oócitos bovinos. No primeiro experimento o LFe foi empregado como meio de manutenção de oócitos bovinos imaturos. No segundo experimento, o meio de maturação foi suplementado com diferentes concentrações de LFe. Os oócitos de ambos os experimentos foram obtidos de ovários coletados em abatedouro. Para o Experimento I, 1228 complexos cumulus-oócitos (CCO’s) foram distribuídos (13 repetições) em 3 tratamentos: T1, T2 e T3 e um grupo controle (C). No grupo controle e no T1, os oócitos foram maturados in vitro (MIV) por 24 e 18h, respectivamente. Os CCO’s do T2 e T3 foram expostos por 6h a 30ºC ao LFe e ao Líquido Folicular bovino (LFb), respectivamente, maturados por 18h. Para a MIV utilizou-se TCM 199. No Experimento II empregou-se 1045 CCO`s, distribuídos (11 repetições) em 3 tratamentos: T1, T2 e T3 e um grupo controle (C). O meio base de maturação fo TCM-199 acrescido de piruvato de sódio, rFSHh e LHe. Esse meio base foi suplementado com 10% de soro de égua em estro para o grupo controle, e 5, 10, e 20% de LFe para os tratamentos T1, T2 e T3, respectivamente. Os CCO`s foram submetidos a MIV por 24 horas. A fecundação in vitro (FIV) e o cultivo foram realizados sob as mesmas condições para os dois experimentos. A FIV foi realizada em meio Talp-Fert. A MIV e a FIV foram realizadas em estufa de cultivo a 39ºC com 5% CO2 em ar e umidade saturada. Os zigotos foram cultivados em meio SOFaaci, sob óleo mineral no interior de bolsas plásticas gaseificadas (Bag System). No Experimento I, a taxa de clivagem foi menor nos CCO´s maturados por 18h (T1, T2 e T3) do que por 24h (C). Não foram observadas diferenças nas taxas de blastocistos em D7 e de eclosão em D9. Quando a MIV foi de 18h, a taxa de blastocistos em D9 foi melhor naqueles oócitos expostos ao Líquido Folicular. No Experimento II, não foram observadas diferenças nas taxas de clivagem e eclosão. A produção de blastocistos no D7 e no D9 do T2 foi superior ao T1 (P<0,05). O LFe pode ser empregado na manutenção e maturação in vitro de oócitos bovinos proporcionando taxas de desenvolvimnto embrionário in vitro semlhantes aquelas obtidas nos grupos controle.Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqporUniversidade Federal de Santa MariaCentro de Ciências RuraisPrograma de Pós-Graduação em Medicina VeterináriaUFSMBrasilMedicina VeterináriaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessOócitos bovinosFluido folicular eqüinoMaturação in vitroBovine oocytesEquine follicular fluidIn vitro maturationCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIALíquido folicular eqüino na produção in vitro de embriões bovinosEquine follicular fluid on ivp of bovine embryosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisSilva, Carlos Antonio Mondinohttp://lattes.cnpq.br/8811861305924545Rubin, Mara Iolanda BatistellaRheingantz, Maria Gabriela Tavareshttp://lattes.cnpq.br/1927422559726593Pinto, Maicon Gaissler Lorena500500000007600600600600600903ff6ef-b847-4354-89fa-8bcae7add9e03f3cdca7-6510-4534-b65d-b8a1633a9bb52d43758b-8656-4b94-9584-381861ecb1d22bd07789-e997-4b5d-a6cb-4db8ac12603freponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDIS_PPGMV_2002_PINTO_MAICON.pdfDIS_PPGMV_2002_PINTO_MAICON.pdfDissertação de mestradoapplication/pdf572519http://repositorio.ufsm.br/bitstream/1/26712/1/DIS_PPGMV_2002_PINTO_MAICON.pdff503aca43c8ae8cb2dd0def7748def9bMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/26712/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81956http://repositorio.ufsm.br/bitstream/1/26712/3/license.txt2f0571ecee68693bd5cd3f17c1e075dfMD531/267122022-10-31 15:04:33.911oai:repositorio.ufsm.br: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 Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-10-31T18:04:33Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Líquido folicular eqüino na produção in vitro de embriões bovinos |
dc.title.alternative.eng.fl_str_mv |
Equine follicular fluid on ivp of bovine embryos |
title |
Líquido folicular eqüino na produção in vitro de embriões bovinos |
spellingShingle |
Líquido folicular eqüino na produção in vitro de embriões bovinos Pinto, Maicon Gaissler Lorena Oócitos bovinos Fluido folicular eqüino Maturação in vitro Bovine oocytes Equine follicular fluid In vitro maturation CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Líquido folicular eqüino na produção in vitro de embriões bovinos |
title_full |
Líquido folicular eqüino na produção in vitro de embriões bovinos |
title_fullStr |
Líquido folicular eqüino na produção in vitro de embriões bovinos |
title_full_unstemmed |
Líquido folicular eqüino na produção in vitro de embriões bovinos |
title_sort |
Líquido folicular eqüino na produção in vitro de embriões bovinos |
author |
Pinto, Maicon Gaissler Lorena |
author_facet |
Pinto, Maicon Gaissler Lorena |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Silva, Carlos Antonio Mondino |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8811861305924545 |
dc.contributor.referee1.fl_str_mv |
Rubin, Mara Iolanda Batistella |
dc.contributor.referee2.fl_str_mv |
Rheingantz, Maria Gabriela Tavares |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1927422559726593 |
dc.contributor.author.fl_str_mv |
Pinto, Maicon Gaissler Lorena |
contributor_str_mv |
Silva, Carlos Antonio Mondino Rubin, Mara Iolanda Batistella Rheingantz, Maria Gabriela Tavares |
dc.subject.por.fl_str_mv |
Oócitos bovinos Fluido folicular eqüino Maturação in vitro |
topic |
Oócitos bovinos Fluido folicular eqüino Maturação in vitro Bovine oocytes Equine follicular fluid In vitro maturation CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Bovine oocytes Equine follicular fluid In vitro maturation |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
Follicular fluid has been used for in vitro embryo production, although it offers the risk of disease transmission. The use of heterologous follicular fluid can be an alternative to avoid infection. The effect of equine follicular fluid (eFF) on embryonic development of bovine oocytes was evaluated. In Experiment I (EI), eFF was used as holding medium for immature bovine oocytes. In Experiment II (EII), maturation medium was supplemented with different concentrations of eFF. Oocytes for both experiments were obtained from ovaries collected at abattoir. For Experimennt I, 1228 Cumulus-oocyte complexes (COC´s) were distributed (13 replicate) on 3 treatments: T1, T2 and T3 and a control group (C). COC´s of the Control Group and T1 were matured (IVM) for 24 and 18h, respectively. COC´s of T2 and T3 were exposed, respectively, to eFF and bovine follicular fluid (bFF) for 6h at 30ºC, and matured for 18h. TCM 199 was used for IVM. On EII, 1045 COC`s were distributed (11 replicate) on 3 treatments: T1, T2 e T3 and a control group. TCM-199 with sodium pyruvate, rFSHh and Lhe was used as maturation medium. This basic medium was supplemented with 10% estrous mare serum for the control group, and 5, 10, 20% of eLF to treatments T1, T2 e T3, respectively. The COC´s were in vitro matured (IVM) for 24 hours. In vitro fertilization (IVF) and culture offers the same conditions for both experiments. IVF was performed in TALP-FERT medium. IVM and IVF were carried out in an incubator at 39ºC with 5% CO2 in air and saturated humidity. Zygotes were cultured in SOFaaci medium, under mineral oil in gasified plastic bags (Bag System). In experiment I, cleavage rate was lower in COC´s matured for 18h (T1, T2 and T3) than for 24h (Control). No differences were observed on blastocyst rates on D7 and hatching rates on D9. When oocytes were matured for 18h, blastocyst rates on D9 were higher on those COC´s exposed to Follicular Fluid. In experiment II, no differences were observed on cleavage and hatching rates. Blastocyst production on D7 and D9 were higher in T2 than T1 (P<0,05). Equine follicular fluid can be used for holding and maturing bovine oocytes producing similar rates of in vitro embryo development than that obtained for control group. |
publishDate |
2002 |
dc.date.issued.fl_str_mv |
2002-01-07 |
dc.date.accessioned.fl_str_mv |
2022-10-31T18:04:33Z |
dc.date.available.fl_str_mv |
2022-10-31T18:04:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/26712 |
url |
http://repositorio.ufsm.br/handle/1/26712 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
500500000007 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 600 |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Rurais |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Medicina Veterinária |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Medicina Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Rurais |
dc.source.none.fl_str_mv |
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