Anti-idiotypic antibodies in patients with different clinical forms of paracoccidioidomycosis
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/26255 http://dx.doi.org/10.1128/CDLI.7.2.175-181.2000 |
Resumo: | Paracoccidioidomycosis (PCM) is the most prevalent systemic mycosis in Latin America. Patients with PCM show a wide spectrum of clinical and pathological manifestations depending on both host and pathogen factors. Two clinical forms of the disease are recognized: the acute or juvenile form and the chronic or adult form. the major antigenic component of the parasite is a glycoprotein of 43 kDa (gp43). All patient sera present antibodies against gp43 (anti-gp43) and, as demonstrated before by our group, spontaneous anti-idiotypic (anti-Id) antibodies (Ab2) can be detected in patient sera with high titers of anti-gp43. Since it has been postulated that anti-Id antibodies may have a modulating function, we decided to purify and characterize anti-Id antibodies in this system. the possible correlation of Ab2 titers with different clinical forms of disease was also verified. Results showed that purified human anti-Id antibodies (human Ab2) recognized specifically the idiotype of some murine monoclonal anti-gp43 (17c and 3e) but not others (40.d7, 27a, and 8a). Spontaneous anti-Id antibodies were found in all clinical forms of disease. the majority of patients (88%, n = 8) with the acute form of PCM had high titers of Ab2. However, among patients with the multifocal chronic form of the disease, only 29% (n = 14) had high titers of Ab2; 70% (n = 10) of patients with the unifocal chronic form had low titers of Ab2. A correlation between Ab2 titers and anti-gp43 titers was observed before and during antimycotic treatment. Our results suggest that titers of anti-Id antibodies correlate with the severity of PCM in humans. |
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Souza, A. R. [UNIFESP]Gesztesi, J. L. [UNIFESP]del Negro, GMBBenard, G.Sato, J. [UNIFESP]Santos, MVB [UNIFESP]Abrahao, T. B. [UNIFESP]Lopes, J. D. [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)2016-01-24T12:31:01Z2016-01-24T12:31:01Z2000-03-01Clinical and Diagnostic Laboratory Immunology. Washington: Amer Soc Microbiology, v. 7, n. 2, p. 175-181, 2000.1071-412Xhttp://repositorio.unifesp.br/handle/11600/26255http://dx.doi.org/10.1128/CDLI.7.2.175-181.2000WOS000085699100007.pdf10.1128/CDLI.7.2.175-181.2000WOS:000085699100007Paracoccidioidomycosis (PCM) is the most prevalent systemic mycosis in Latin America. Patients with PCM show a wide spectrum of clinical and pathological manifestations depending on both host and pathogen factors. Two clinical forms of the disease are recognized: the acute or juvenile form and the chronic or adult form. the major antigenic component of the parasite is a glycoprotein of 43 kDa (gp43). All patient sera present antibodies against gp43 (anti-gp43) and, as demonstrated before by our group, spontaneous anti-idiotypic (anti-Id) antibodies (Ab2) can be detected in patient sera with high titers of anti-gp43. Since it has been postulated that anti-Id antibodies may have a modulating function, we decided to purify and characterize anti-Id antibodies in this system. the possible correlation of Ab2 titers with different clinical forms of disease was also verified. Results showed that purified human anti-Id antibodies (human Ab2) recognized specifically the idiotype of some murine monoclonal anti-gp43 (17c and 3e) but not others (40.d7, 27a, and 8a). Spontaneous anti-Id antibodies were found in all clinical forms of disease. the majority of patients (88%, n = 8) with the acute form of PCM had high titers of Ab2. However, among patients with the multifocal chronic form of the disease, only 29% (n = 14) had high titers of Ab2; 70% (n = 10) of patients with the unifocal chronic form had low titers of Ab2. A correlation between Ab2 titers and anti-gp43 titers was observed before and during antimycotic treatment. Our results suggest that titers of anti-Id antibodies correlate with the severity of PCM in humans.Universidade Federal de São Paulo, Disciplina Imunol, Dept Microbiol Imunol & Parasitol, BR-04023900 São Paulo, BrazilUniv São Paulo, Sch Med, Med Mycol Lab, Inst Med Trop São Paulo, São Paulo, BrazilUniv São Paulo, Sch Med, Lab Med Invest 56, São Paulo, BrazilUniversidade Federal de São Paulo, Disciplina Imunol, Dept Microbiol Imunol & Parasitol, BR-04023900 São Paulo, BrazilWeb of Science175-181engAmer Soc MicrobiologyClinical and Diagnostic Laboratory ImmunologyAnti-idiotypic antibodies in patients with different clinical forms of paracoccidioidomycosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000085699100007.pdfapplication/pdf109000${dspace.ui.url}/bitstream/11600/26255/1/WOS000085699100007.pdf89917529290f3f01015f1a72941865d6MD51open accessTEXTWOS000085699100007.pdf.txtWOS000085699100007.pdf.txtExtracted texttext/plain42085${dspace.ui.url}/bitstream/11600/26255/2/WOS000085699100007.pdf.txt9e288ebb2690a92a0d4676afe74db992MD52open access11600/262552022-09-27 11:18:56.015open accessoai:repositorio.unifesp.br:11600/26255Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:25:10.022545Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Anti-idiotypic antibodies in patients with different clinical forms of paracoccidioidomycosis |
title |
Anti-idiotypic antibodies in patients with different clinical forms of paracoccidioidomycosis |
spellingShingle |
Anti-idiotypic antibodies in patients with different clinical forms of paracoccidioidomycosis Souza, A. R. [UNIFESP] |
title_short |
Anti-idiotypic antibodies in patients with different clinical forms of paracoccidioidomycosis |
title_full |
Anti-idiotypic antibodies in patients with different clinical forms of paracoccidioidomycosis |
title_fullStr |
Anti-idiotypic antibodies in patients with different clinical forms of paracoccidioidomycosis |
title_full_unstemmed |
Anti-idiotypic antibodies in patients with different clinical forms of paracoccidioidomycosis |
title_sort |
Anti-idiotypic antibodies in patients with different clinical forms of paracoccidioidomycosis |
author |
Souza, A. R. [UNIFESP] |
author_facet |
Souza, A. R. [UNIFESP] Gesztesi, J. L. [UNIFESP] del Negro, GMB Benard, G. Sato, J. [UNIFESP] Santos, MVB [UNIFESP] Abrahao, T. B. [UNIFESP] Lopes, J. D. [UNIFESP] |
author_role |
author |
author2 |
Gesztesi, J. L. [UNIFESP] del Negro, GMB Benard, G. Sato, J. [UNIFESP] Santos, MVB [UNIFESP] Abrahao, T. B. [UNIFESP] Lopes, J. D. [UNIFESP] |
author2_role |
author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Souza, A. R. [UNIFESP] Gesztesi, J. L. [UNIFESP] del Negro, GMB Benard, G. Sato, J. [UNIFESP] Santos, MVB [UNIFESP] Abrahao, T. B. [UNIFESP] Lopes, J. D. [UNIFESP] |
description |
Paracoccidioidomycosis (PCM) is the most prevalent systemic mycosis in Latin America. Patients with PCM show a wide spectrum of clinical and pathological manifestations depending on both host and pathogen factors. Two clinical forms of the disease are recognized: the acute or juvenile form and the chronic or adult form. the major antigenic component of the parasite is a glycoprotein of 43 kDa (gp43). All patient sera present antibodies against gp43 (anti-gp43) and, as demonstrated before by our group, spontaneous anti-idiotypic (anti-Id) antibodies (Ab2) can be detected in patient sera with high titers of anti-gp43. Since it has been postulated that anti-Id antibodies may have a modulating function, we decided to purify and characterize anti-Id antibodies in this system. the possible correlation of Ab2 titers with different clinical forms of disease was also verified. Results showed that purified human anti-Id antibodies (human Ab2) recognized specifically the idiotype of some murine monoclonal anti-gp43 (17c and 3e) but not others (40.d7, 27a, and 8a). Spontaneous anti-Id antibodies were found in all clinical forms of disease. the majority of patients (88%, n = 8) with the acute form of PCM had high titers of Ab2. However, among patients with the multifocal chronic form of the disease, only 29% (n = 14) had high titers of Ab2; 70% (n = 10) of patients with the unifocal chronic form had low titers of Ab2. A correlation between Ab2 titers and anti-gp43 titers was observed before and during antimycotic treatment. Our results suggest that titers of anti-Id antibodies correlate with the severity of PCM in humans. |
publishDate |
2000 |
dc.date.issued.fl_str_mv |
2000-03-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T12:31:01Z |
dc.date.available.fl_str_mv |
2016-01-24T12:31:01Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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dc.identifier.citation.fl_str_mv |
Clinical and Diagnostic Laboratory Immunology. Washington: Amer Soc Microbiology, v. 7, n. 2, p. 175-181, 2000. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/26255 http://dx.doi.org/10.1128/CDLI.7.2.175-181.2000 |
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1071-412X |
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WOS000085699100007.pdf |
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10.1128/CDLI.7.2.175-181.2000 |
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WOS:000085699100007 |
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Clinical and Diagnostic Laboratory Immunology. Washington: Amer Soc Microbiology, v. 7, n. 2, p. 175-181, 2000. 1071-412X WOS000085699100007.pdf 10.1128/CDLI.7.2.175-181.2000 WOS:000085699100007 |
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http://repositorio.unifesp.br/handle/11600/26255 http://dx.doi.org/10.1128/CDLI.7.2.175-181.2000 |
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