Placental transfer of IgG and IgG subclass antibodies anti-purified Escherichia coli LPS 016, 06 and 0111
Autor(a) principal: | |
---|---|
Data de Publicação: | 1998 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/11600/45567 http://dx.doi.org/10.1046/j.1365-3083.1998.00353.x |
Resumo: | We evaluated 22 paired maternal and cord sera regarding the presence of IgG and IgG subclasses against purified Escherichia coli LPS O6, O16 and O111 employing ELISA for titre and avidity analysis, isoelectric focusing associated with affinity-blotting for spectrotypic analysis, and the Western-blotting technique for recognition of the various bands in lipopolysaccharide (LPS). Levels of anti-LPS IgG antibodies in cord sera were equivalent to their respective maternal sera, showing a significant correlation (P<0.0001). IgG1 antibody levels were higher in cord sera than in maternal sera (P < 0.005 for anti-O111, P < 0.05 for anti-O16 and P < 0.02 for anti-O6). Cord IgG2 antibody levels were not different from the maternal levels (P > 0.1). The levels of IgG3 and IgG4 were undetectable. The avidity of anti-O6 and anti-Olll IgG in 10 cord sera showed an extremely significant correlation with maternal antibody avidity (P<0.0001). Identical patterns of recognition were found in the paired samples analysed by Western blotting. Most of the serum samples recognized the O-repetitive chains and also the region corresponding to core and Lipid A. Although the antibody spectrotypes varied among individuals, paired cord and maternal serum samples showed identical patterns. Our findings suggest the occurrence of placental transfer of IgG antibodies against LPS O6, O16 and O111, mainly involving the IgG1 or IgG2 subclasses. |
id |
UFSP_15b9091b3d7bde66ac85068ac342dfa0 |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br:11600/45567 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
Nagao-Dias, Aparecida TiemiMartinez, C. C.Vieira, V. S.Takano, Olga AkikoCosta-Carvalho, Beatriz Tavares [UNIFESP]Carneiro-Sampaio, Magda Maria SalesUniversidade de São Paulo (USP)Inst Trop MedInst Adolfo LutzUniversidade Federal de São Paulo (UNIFESP)2018-06-18T12:11:51Z2018-06-18T12:11:51Z1998-06-01Scandinavian Journal Of Immunology. Oxford: Blackwell Science Ltd, v. 47, n. 6, p. 609-614, 1998.0300-9475http://repositorio.unifesp.br/11600/45567http://dx.doi.org/10.1046/j.1365-3083.1998.00353.x10.1046/j.1365-3083.1998.00353.xWOS:000074337600013We evaluated 22 paired maternal and cord sera regarding the presence of IgG and IgG subclasses against purified Escherichia coli LPS O6, O16 and O111 employing ELISA for titre and avidity analysis, isoelectric focusing associated with affinity-blotting for spectrotypic analysis, and the Western-blotting technique for recognition of the various bands in lipopolysaccharide (LPS). Levels of anti-LPS IgG antibodies in cord sera were equivalent to their respective maternal sera, showing a significant correlation (P<0.0001). IgG1 antibody levels were higher in cord sera than in maternal sera (P < 0.005 for anti-O111, P < 0.05 for anti-O16 and P < 0.02 for anti-O6). Cord IgG2 antibody levels were not different from the maternal levels (P > 0.1). The levels of IgG3 and IgG4 were undetectable. The avidity of anti-O6 and anti-Olll IgG in 10 cord sera showed an extremely significant correlation with maternal antibody avidity (P<0.0001). Identical patterns of recognition were found in the paired samples analysed by Western blotting. Most of the serum samples recognized the O-repetitive chains and also the region corresponding to core and Lipid A. Although the antibody spectrotypes varied among individuals, paired cord and maternal serum samples showed identical patterns. Our findings suggest the occurrence of placental transfer of IgG antibodies against LPS O6, O16 and O111, mainly involving the IgG1 or IgG2 subclasses.Univ Sao Paulo, Dept Immunol, Inst Ciencias Biomed, BR-05508900 Sao Paulo, SP, BrazilInst Trop Med, Asuncion, ParaguayInst Adolfo Lutz, Sao Paulo, BrazilUniv Fed Mato Grosso, Dept Pediat, Sao Paulo, BrazilUniv Fed Sao Paulo, Sao Paulo, BrazilUniv Fed Sao Paulo, Sao Paulo, BrazilWeb of Science609-614engBlackwell Science LtdScandinavian Journal Of ImmunologyPlacental transfer of IgG and IgG subclass antibodies anti-purified Escherichia coli LPS 016, 06 and 0111info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/455672022-02-07 20:58:47.538metadata only accessoai:repositorio.unifesp.br:11600/45567Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:21:34.476012Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Placental transfer of IgG and IgG subclass antibodies anti-purified Escherichia coli LPS 016, 06 and 0111 |
title |
Placental transfer of IgG and IgG subclass antibodies anti-purified Escherichia coli LPS 016, 06 and 0111 |
spellingShingle |
Placental transfer of IgG and IgG subclass antibodies anti-purified Escherichia coli LPS 016, 06 and 0111 Nagao-Dias, Aparecida Tiemi |
title_short |
Placental transfer of IgG and IgG subclass antibodies anti-purified Escherichia coli LPS 016, 06 and 0111 |
title_full |
Placental transfer of IgG and IgG subclass antibodies anti-purified Escherichia coli LPS 016, 06 and 0111 |
title_fullStr |
Placental transfer of IgG and IgG subclass antibodies anti-purified Escherichia coli LPS 016, 06 and 0111 |
title_full_unstemmed |
Placental transfer of IgG and IgG subclass antibodies anti-purified Escherichia coli LPS 016, 06 and 0111 |
title_sort |
Placental transfer of IgG and IgG subclass antibodies anti-purified Escherichia coli LPS 016, 06 and 0111 |
author |
Nagao-Dias, Aparecida Tiemi |
author_facet |
Nagao-Dias, Aparecida Tiemi Martinez, C. C. Vieira, V. S. Takano, Olga Akiko Costa-Carvalho, Beatriz Tavares [UNIFESP] Carneiro-Sampaio, Magda Maria Sales |
author_role |
author |
author2 |
Martinez, C. C. Vieira, V. S. Takano, Olga Akiko Costa-Carvalho, Beatriz Tavares [UNIFESP] Carneiro-Sampaio, Magda Maria Sales |
author2_role |
author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade de São Paulo (USP) Inst Trop Med Inst Adolfo Lutz Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Nagao-Dias, Aparecida Tiemi Martinez, C. C. Vieira, V. S. Takano, Olga Akiko Costa-Carvalho, Beatriz Tavares [UNIFESP] Carneiro-Sampaio, Magda Maria Sales |
description |
We evaluated 22 paired maternal and cord sera regarding the presence of IgG and IgG subclasses against purified Escherichia coli LPS O6, O16 and O111 employing ELISA for titre and avidity analysis, isoelectric focusing associated with affinity-blotting for spectrotypic analysis, and the Western-blotting technique for recognition of the various bands in lipopolysaccharide (LPS). Levels of anti-LPS IgG antibodies in cord sera were equivalent to their respective maternal sera, showing a significant correlation (P<0.0001). IgG1 antibody levels were higher in cord sera than in maternal sera (P < 0.005 for anti-O111, P < 0.05 for anti-O16 and P < 0.02 for anti-O6). Cord IgG2 antibody levels were not different from the maternal levels (P > 0.1). The levels of IgG3 and IgG4 were undetectable. The avidity of anti-O6 and anti-Olll IgG in 10 cord sera showed an extremely significant correlation with maternal antibody avidity (P<0.0001). Identical patterns of recognition were found in the paired samples analysed by Western blotting. Most of the serum samples recognized the O-repetitive chains and also the region corresponding to core and Lipid A. Although the antibody spectrotypes varied among individuals, paired cord and maternal serum samples showed identical patterns. Our findings suggest the occurrence of placental transfer of IgG antibodies against LPS O6, O16 and O111, mainly involving the IgG1 or IgG2 subclasses. |
publishDate |
1998 |
dc.date.issued.fl_str_mv |
1998-06-01 |
dc.date.accessioned.fl_str_mv |
2018-06-18T12:11:51Z |
dc.date.available.fl_str_mv |
2018-06-18T12:11:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Scandinavian Journal Of Immunology. Oxford: Blackwell Science Ltd, v. 47, n. 6, p. 609-614, 1998. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/11600/45567 http://dx.doi.org/10.1046/j.1365-3083.1998.00353.x |
dc.identifier.issn.none.fl_str_mv |
0300-9475 |
dc.identifier.doi.none.fl_str_mv |
10.1046/j.1365-3083.1998.00353.x |
dc.identifier.wos.none.fl_str_mv |
WOS:000074337600013 |
identifier_str_mv |
Scandinavian Journal Of Immunology. Oxford: Blackwell Science Ltd, v. 47, n. 6, p. 609-614, 1998. 0300-9475 10.1046/j.1365-3083.1998.00353.x WOS:000074337600013 |
url |
http://repositorio.unifesp.br/11600/45567 http://dx.doi.org/10.1046/j.1365-3083.1998.00353.x |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Scandinavian Journal Of Immunology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
609-614 |
dc.publisher.none.fl_str_mv |
Blackwell Science Ltd |
publisher.none.fl_str_mv |
Blackwell Science Ltd |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1783460280799330304 |