Differential Infectivity by the Oral Route of Trypanosoma cruzi Lineages Derived from Y Strain
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/35323 http://dx.doi.org/10.1371/journal.pntd.0001804 |
Resumo: | Background: Diversity of T. cruzi strains is a central problem in Chagas disease research because of its correlation with the wide range of clinical manifestations and the biogeographical parasite distribution. the role played by parasite microdiversity in Chagas disease epidemiology is still debatable. Also awaits clarification whether such diversity is associated with the outcome of oral T. cruzi infection, responsible for frequent outbreaks of acute Chagas disease.Methods and Findings: We addressed the impact of microdiversity in oral T. cruzi infection, by comparative analysis of two strains, Y30 and Y82, both derived from Y strain, a widely used experimental model. Network genealogies of four nuclear genes (SSU rDNA, actin, DHFR-TS, EF1 alpha) revealed that Y30 is closely related to Discrete Typing Unit TcII while Y82 is more closely related to TcVI, a group containing hybrid strains. Nevertheless, excepting one A-G transition at position 1463, Y30 and Y82 SSU rDNAs were identical. Y82 strain, expressing the surface molecule gp82, infected mice orally more efficiently than Y30, which expresses a related gp30 molecule. Both molecules are involved in lysosome exocytosis-dependent host cell invasion, but exhibit differential gastric mucin-binding capacity, a property critical for parasite migration toward the gastric mucosal epithelium. Upon oral infection of mice, the number of Y30 and Y82 parasites in gastric epithelial cells differed widely.Conclusions: We conclude that metacyclic forms of gp82-expressing Y82 strain, closely related to TcVI, are better adapted than Y30 strain (TcII) to traverse the stomach mucous layer and establish oral route infection. the efficiency to infect target cell is the same because gp82 and gp30 strains have similar invasion-promoting properties. Unknown is whether differences in Y30 and Y82 are natural parasite adaptations or a product of lab-induced evolution by differential selection along the 60 years elapsed since the Y strain isolation. |
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Cortez, Cristian [UNIFESP]Martins, Rafael M. [UNIFESP]Alves, Renan M. [UNIFESP]Silva, Richard C. [UNIFESP]Bilches, Luciana C. [UNIFESP]Macedo, Silene [UNIFESP]Atayde, Vanessa [UNIFESP]Kawashita, Silvia Y. [UNIFESP]Briones, Marcelo R. S. [UNIFESP]Yoshida, Nobuko [UNIFESP]Universidade Federal de São Paulo (UNIFESP)McGill UnivInst PasteurSuperintendencia Policia Tecn Cient2016-01-24T14:27:45Z2016-01-24T14:27:45Z2012-10-01Plos Neglected Tropical Diseases. San Francisco: Public Library Science, v. 6, n. 10, 13 p., 2012.1935-2735http://repositorio.unifesp.br/handle/11600/35323http://dx.doi.org/10.1371/journal.pntd.0001804WOS000310527200003.pdf10.1371/journal.pntd.0001804WOS:000310527200003Background: Diversity of T. cruzi strains is a central problem in Chagas disease research because of its correlation with the wide range of clinical manifestations and the biogeographical parasite distribution. the role played by parasite microdiversity in Chagas disease epidemiology is still debatable. Also awaits clarification whether such diversity is associated with the outcome of oral T. cruzi infection, responsible for frequent outbreaks of acute Chagas disease.Methods and Findings: We addressed the impact of microdiversity in oral T. cruzi infection, by comparative analysis of two strains, Y30 and Y82, both derived from Y strain, a widely used experimental model. Network genealogies of four nuclear genes (SSU rDNA, actin, DHFR-TS, EF1 alpha) revealed that Y30 is closely related to Discrete Typing Unit TcII while Y82 is more closely related to TcVI, a group containing hybrid strains. Nevertheless, excepting one A-G transition at position 1463, Y30 and Y82 SSU rDNAs were identical. Y82 strain, expressing the surface molecule gp82, infected mice orally more efficiently than Y30, which expresses a related gp30 molecule. Both molecules are involved in lysosome exocytosis-dependent host cell invasion, but exhibit differential gastric mucin-binding capacity, a property critical for parasite migration toward the gastric mucosal epithelium. Upon oral infection of mice, the number of Y30 and Y82 parasites in gastric epithelial cells differed widely.Conclusions: We conclude that metacyclic forms of gp82-expressing Y82 strain, closely related to TcVI, are better adapted than Y30 strain (TcII) to traverse the stomach mucous layer and establish oral route infection. the efficiency to infect target cell is the same because gp82 and gp30 strains have similar invasion-promoting properties. Unknown is whether differences in Y30 and Y82 are natural parasite adaptations or a product of lab-induced evolution by differential selection along the 60 years elapsed since the Y strain isolation.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilMcGill Univ, Ctr Hlth, Montreal, PQ, CanadaInst Pasteur, Unite Biol Interact Hote Parasite, Paris, FranceSuperintendencia Policia Tecn Cient, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilFAPESP: 2006/61450-0CNPq: 301409/2007-2CNPq: 470726/2007-5Web of Science13engPublic Library SciencePlos Neglected Tropical DiseasesDifferential Infectivity by the Oral Route of Trypanosoma cruzi Lineages Derived from Y Straininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000310527200003.pdfapplication/pdf2868635${dspace.ui.url}/bitstream/11600/35323/1/WOS000310527200003.pdf55577e0f11f0da4e0cf66548e07f7946MD51open accessTEXTWOS000310527200003.pdf.txtWOS000310527200003.pdf.txtExtracted texttext/plain63136${dspace.ui.url}/bitstream/11600/35323/2/WOS000310527200003.pdf.txt572b63fa2bcb71b0786fb44de15f1600MD52open access11600/353232022-11-04 15:40:32.606open accessoai:repositorio.unifesp.br:11600/35323Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:29:39.124373Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Differential Infectivity by the Oral Route of Trypanosoma cruzi Lineages Derived from Y Strain |
title |
Differential Infectivity by the Oral Route of Trypanosoma cruzi Lineages Derived from Y Strain |
spellingShingle |
Differential Infectivity by the Oral Route of Trypanosoma cruzi Lineages Derived from Y Strain Cortez, Cristian [UNIFESP] |
title_short |
Differential Infectivity by the Oral Route of Trypanosoma cruzi Lineages Derived from Y Strain |
title_full |
Differential Infectivity by the Oral Route of Trypanosoma cruzi Lineages Derived from Y Strain |
title_fullStr |
Differential Infectivity by the Oral Route of Trypanosoma cruzi Lineages Derived from Y Strain |
title_full_unstemmed |
Differential Infectivity by the Oral Route of Trypanosoma cruzi Lineages Derived from Y Strain |
title_sort |
Differential Infectivity by the Oral Route of Trypanosoma cruzi Lineages Derived from Y Strain |
author |
Cortez, Cristian [UNIFESP] |
author_facet |
Cortez, Cristian [UNIFESP] Martins, Rafael M. [UNIFESP] Alves, Renan M. [UNIFESP] Silva, Richard C. [UNIFESP] Bilches, Luciana C. [UNIFESP] Macedo, Silene [UNIFESP] Atayde, Vanessa [UNIFESP] Kawashita, Silvia Y. [UNIFESP] Briones, Marcelo R. S. [UNIFESP] Yoshida, Nobuko [UNIFESP] |
author_role |
author |
author2 |
Martins, Rafael M. [UNIFESP] Alves, Renan M. [UNIFESP] Silva, Richard C. [UNIFESP] Bilches, Luciana C. [UNIFESP] Macedo, Silene [UNIFESP] Atayde, Vanessa [UNIFESP] Kawashita, Silvia Y. [UNIFESP] Briones, Marcelo R. S. [UNIFESP] Yoshida, Nobuko [UNIFESP] |
author2_role |
author author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) McGill Univ Inst Pasteur Superintendencia Policia Tecn Cient |
dc.contributor.author.fl_str_mv |
Cortez, Cristian [UNIFESP] Martins, Rafael M. [UNIFESP] Alves, Renan M. [UNIFESP] Silva, Richard C. [UNIFESP] Bilches, Luciana C. [UNIFESP] Macedo, Silene [UNIFESP] Atayde, Vanessa [UNIFESP] Kawashita, Silvia Y. [UNIFESP] Briones, Marcelo R. S. [UNIFESP] Yoshida, Nobuko [UNIFESP] |
description |
Background: Diversity of T. cruzi strains is a central problem in Chagas disease research because of its correlation with the wide range of clinical manifestations and the biogeographical parasite distribution. the role played by parasite microdiversity in Chagas disease epidemiology is still debatable. Also awaits clarification whether such diversity is associated with the outcome of oral T. cruzi infection, responsible for frequent outbreaks of acute Chagas disease.Methods and Findings: We addressed the impact of microdiversity in oral T. cruzi infection, by comparative analysis of two strains, Y30 and Y82, both derived from Y strain, a widely used experimental model. Network genealogies of four nuclear genes (SSU rDNA, actin, DHFR-TS, EF1 alpha) revealed that Y30 is closely related to Discrete Typing Unit TcII while Y82 is more closely related to TcVI, a group containing hybrid strains. Nevertheless, excepting one A-G transition at position 1463, Y30 and Y82 SSU rDNAs were identical. Y82 strain, expressing the surface molecule gp82, infected mice orally more efficiently than Y30, which expresses a related gp30 molecule. Both molecules are involved in lysosome exocytosis-dependent host cell invasion, but exhibit differential gastric mucin-binding capacity, a property critical for parasite migration toward the gastric mucosal epithelium. Upon oral infection of mice, the number of Y30 and Y82 parasites in gastric epithelial cells differed widely.Conclusions: We conclude that metacyclic forms of gp82-expressing Y82 strain, closely related to TcVI, are better adapted than Y30 strain (TcII) to traverse the stomach mucous layer and establish oral route infection. the efficiency to infect target cell is the same because gp82 and gp30 strains have similar invasion-promoting properties. Unknown is whether differences in Y30 and Y82 are natural parasite adaptations or a product of lab-induced evolution by differential selection along the 60 years elapsed since the Y strain isolation. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-10-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:27:45Z |
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2016-01-24T14:27:45Z |
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info:eu-repo/semantics/publishedVersion |
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article |
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dc.identifier.citation.fl_str_mv |
Plos Neglected Tropical Diseases. San Francisco: Public Library Science, v. 6, n. 10, 13 p., 2012. |
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http://repositorio.unifesp.br/handle/11600/35323 http://dx.doi.org/10.1371/journal.pntd.0001804 |
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1935-2735 |
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WOS000310527200003.pdf |
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10.1371/journal.pntd.0001804 |
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Plos Neglected Tropical Diseases. San Francisco: Public Library Science, v. 6, n. 10, 13 p., 2012. 1935-2735 WOS000310527200003.pdf 10.1371/journal.pntd.0001804 WOS:000310527200003 |
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http://repositorio.unifesp.br/handle/11600/35323 http://dx.doi.org/10.1371/journal.pntd.0001804 |
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