The low efficiency of dendritic cells and macrophages from mice susceptible to Paracoccidioides brasiliensis in inducing a Th1 response
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/1132 http://dx.doi.org/10.1590/S0100-879X2001000400014 |
Resumo: | In the present study we evaluated T cell proliferation and Th lymphokine patterns in response to gp43 from Paracoccidioides brasiliensis presented by isolated dendritic cells from susceptible and resistant mice. T cell proliferation assays showed that dendritic cells from susceptible mice were less efficient than those from resistant mice. The pattern of T cell lymphokines stimulated by dendritic cells was always Th1, although the levels of IL-2 and IFN-gamma were lower in T cell cultures from susceptible mice. To determie whether different antigen-presenting cells such as macrophages and dendritic cells stimulated different concentrations of Th1 lymphokines, the production of IFN-gamma and IL-2 was measured. It was observed that dendritic cells were more efficient than macrophages in stimulating lymphoproliferation in resistant mice. However, no significant difference was observed for IFN-gamma or IL-2 production. When cells from susceptible mice were used, macrophages were more efficient in stimulating lymphoproliferation than dendritic cells, but no difference was observed in the production of Th1 cytokine. Taken together, these results suggest the lower efficiency of dendritic cells and macrophages from B10.A mice in stimulating T cells that secrete Th1 lymphokines in vitro, an effect that may be involved in the progression of the disease in vivo. |
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Almeida, S.r. [UNIFESP]Lopes, Jose Daniel [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2015-06-14T13:29:21Z2015-06-14T13:29:21Z2001-04-01Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 4, p. 529-537, 2001.0100-879Xhttp://repositorio.unifesp.br/handle/11600/1132http://dx.doi.org/10.1590/S0100-879X2001000400014S0100-879X2001000400014.pdfS0100-879X200100040001410.1590/S0100-879X2001000400014WOS:000168205400014In the present study we evaluated T cell proliferation and Th lymphokine patterns in response to gp43 from Paracoccidioides brasiliensis presented by isolated dendritic cells from susceptible and resistant mice. T cell proliferation assays showed that dendritic cells from susceptible mice were less efficient than those from resistant mice. The pattern of T cell lymphokines stimulated by dendritic cells was always Th1, although the levels of IL-2 and IFN-gamma were lower in T cell cultures from susceptible mice. To determie whether different antigen-presenting cells such as macrophages and dendritic cells stimulated different concentrations of Th1 lymphokines, the production of IFN-gamma and IL-2 was measured. It was observed that dendritic cells were more efficient than macrophages in stimulating lymphoproliferation in resistant mice. However, no significant difference was observed for IFN-gamma or IL-2 production. When cells from susceptible mice were used, macrophages were more efficient in stimulating lymphoproliferation than dendritic cells, but no difference was observed in the production of Th1 cytokine. Taken together, these results suggest the lower efficiency of dendritic cells and macrophages from B10.A mice in stimulating T cells that secrete Th1 lymphokines in vitro, an effect that may be involved in the progression of the disease in vivo.Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Microbiologia, Imunologia e ParasitologiaUNIFESP, EPM, Depto. de Microbiologia, Imunologia e ParasitologiaSciELO529-537engAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Researchdendritic cellsparacoccidioidomycosisParacoccidioides brasiliensisTh1/Th2The low efficiency of dendritic cells and macrophages from mice susceptible to Paracoccidioides brasiliensis in inducing a Th1 responseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS0100-879X2001000400014.pdfapplication/pdf170027${dspace.ui.url}/bitstream/11600/1132/1/S0100-879X2001000400014.pdf53c9b2be8f1e44619fe47bf4909fdd60MD51open accessTEXTS0100-879X2001000400014.pdf.txtS0100-879X2001000400014.pdf.txtExtracted texttext/plain35260${dspace.ui.url}/bitstream/11600/1132/2/S0100-879X2001000400014.pdf.txt2a3b79a7800c93d718bcc214862977a0MD52open access11600/11322022-02-08 11:52:12.077open accessoai:repositorio.unifesp.br:11600/1132Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:20:24.906622Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
The low efficiency of dendritic cells and macrophages from mice susceptible to Paracoccidioides brasiliensis in inducing a Th1 response |
title |
The low efficiency of dendritic cells and macrophages from mice susceptible to Paracoccidioides brasiliensis in inducing a Th1 response |
spellingShingle |
The low efficiency of dendritic cells and macrophages from mice susceptible to Paracoccidioides brasiliensis in inducing a Th1 response Almeida, S.r. [UNIFESP] dendritic cells paracoccidioidomycosis Paracoccidioides brasiliensis Th1/Th2 |
title_short |
The low efficiency of dendritic cells and macrophages from mice susceptible to Paracoccidioides brasiliensis in inducing a Th1 response |
title_full |
The low efficiency of dendritic cells and macrophages from mice susceptible to Paracoccidioides brasiliensis in inducing a Th1 response |
title_fullStr |
The low efficiency of dendritic cells and macrophages from mice susceptible to Paracoccidioides brasiliensis in inducing a Th1 response |
title_full_unstemmed |
The low efficiency of dendritic cells and macrophages from mice susceptible to Paracoccidioides brasiliensis in inducing a Th1 response |
title_sort |
The low efficiency of dendritic cells and macrophages from mice susceptible to Paracoccidioides brasiliensis in inducing a Th1 response |
author |
Almeida, S.r. [UNIFESP] |
author_facet |
Almeida, S.r. [UNIFESP] Lopes, Jose Daniel [UNIFESP] |
author_role |
author |
author2 |
Lopes, Jose Daniel [UNIFESP] |
author2_role |
author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Almeida, S.r. [UNIFESP] Lopes, Jose Daniel [UNIFESP] |
dc.subject.eng.fl_str_mv |
dendritic cells paracoccidioidomycosis Paracoccidioides brasiliensis Th1/Th2 |
topic |
dendritic cells paracoccidioidomycosis Paracoccidioides brasiliensis Th1/Th2 |
description |
In the present study we evaluated T cell proliferation and Th lymphokine patterns in response to gp43 from Paracoccidioides brasiliensis presented by isolated dendritic cells from susceptible and resistant mice. T cell proliferation assays showed that dendritic cells from susceptible mice were less efficient than those from resistant mice. The pattern of T cell lymphokines stimulated by dendritic cells was always Th1, although the levels of IL-2 and IFN-gamma were lower in T cell cultures from susceptible mice. To determie whether different antigen-presenting cells such as macrophages and dendritic cells stimulated different concentrations of Th1 lymphokines, the production of IFN-gamma and IL-2 was measured. It was observed that dendritic cells were more efficient than macrophages in stimulating lymphoproliferation in resistant mice. However, no significant difference was observed for IFN-gamma or IL-2 production. When cells from susceptible mice were used, macrophages were more efficient in stimulating lymphoproliferation than dendritic cells, but no difference was observed in the production of Th1 cytokine. Taken together, these results suggest the lower efficiency of dendritic cells and macrophages from B10.A mice in stimulating T cells that secrete Th1 lymphokines in vitro, an effect that may be involved in the progression of the disease in vivo. |
publishDate |
2001 |
dc.date.issued.fl_str_mv |
2001-04-01 |
dc.date.accessioned.fl_str_mv |
2015-06-14T13:29:21Z |
dc.date.available.fl_str_mv |
2015-06-14T13:29:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 4, p. 529-537, 2001. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/1132 http://dx.doi.org/10.1590/S0100-879X2001000400014 |
dc.identifier.issn.none.fl_str_mv |
0100-879X |
dc.identifier.file.none.fl_str_mv |
S0100-879X2001000400014.pdf |
dc.identifier.scielo.none.fl_str_mv |
S0100-879X2001000400014 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S0100-879X2001000400014 |
dc.identifier.wos.none.fl_str_mv |
WOS:000168205400014 |
identifier_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 4, p. 529-537, 2001. 0100-879X S0100-879X2001000400014.pdf S0100-879X2001000400014 10.1590/S0100-879X2001000400014 WOS:000168205400014 |
url |
http://repositorio.unifesp.br/handle/11600/1132 http://dx.doi.org/10.1590/S0100-879X2001000400014 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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Brazilian Journal of Medical and Biological Research |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
529-537 |
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Associação Brasileira de Divulgação Científica |
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Associação Brasileira de Divulgação Científica |
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