Treatment of Leishmania (Leishmania) Amazonensis-Infected Mice with a Combination of a Palladacycle Complex and Heat-Killed Propionibacterium acnes Triggers Protective Cellular Immune Responses
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/54937 http://dx.doi.org/10.3389/fmicb.2017.00333 |
Resumo: | Palladacycle complex DPPE 1.2 was previously reported to inhibit the in vitro and in vivo infection by Leishmania (Leishmania) amazonensis. The aim of the present study was to compare the effect of DPPE 1.2, in association with heat-killed Propionibacterium acnes, on L. (L.) amazonensis infection in two mouse strains, BALB/c and C57BL/6, and to evaluate the immune responses of the treated animals. Foot lesions of L. (L.) amazonensis-infected mice were injected with DPPE 1.2 alone, or associated with P. acnes as an adjuvant. Analysis of T-cell populations in the treated mice and in untreated controls was performed by FACS. Detection of IFN-gamma-secreting lymphocytes was carried out by an ELISPOT assay and active TGF-beta was measured by means of a double-sandwich ELISA test. The treatment with DPPE 1.2 resulted in a significant reduction of foot lesion sizes and parasite burdens in both mouse strains, and the lowest parasite burden was found in mice treated with DPPE 1.2 plus P. acnes. Mice treated with DPPE 1.2 alone displayed a significant increase of TCD4(+) and TCD8(+) lymphocytes and IFN-gamma secretion which were significantly higher in animals treated with DPPE 1.2 plus P. acnes. A significant reduction of active TGF-b was observed in mice treated with DPPE 1.2 alone or associated with P. acnes. Moreover, DPPE 1.2 associated to P. acnes was non-toxic to treated animals. The destruction of L. (L.) amazonensis by DPPE 1.2 was followed by host inflammatory responses which were exacerbated when the palladacycle complex was associated with P. acnes. |
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Paladi, Carolina S. [UNIFESP]da Silva, Danielle A. M. [UNIFESP]Motta, Priscila D. [UNIFESP]Garcia, Daniel M. [UNIFESP]Teixeira, Daniela [UNIFESP]Longo-Maugeri, Ieda M. [UNIFESP]Katz, Simone [UNIFESP]Barbieri, Clara L. [UNIFESP]2020-07-17T14:02:40Z2020-07-17T14:02:40Z2017Frontiers In Microbiology. Lausanne, v. 8, p. -, 2017.1664-302Xhttps://repositorio.unifesp.br/handle/11600/54937http://dx.doi.org/10.3389/fmicb.2017.00333WOS000395238500001.pdf10.3389/fmicb.2017.00333WOS:000395238500001Palladacycle complex DPPE 1.2 was previously reported to inhibit the in vitro and in vivo infection by Leishmania (Leishmania) amazonensis. The aim of the present study was to compare the effect of DPPE 1.2, in association with heat-killed Propionibacterium acnes, on L. (L.) amazonensis infection in two mouse strains, BALB/c and C57BL/6, and to evaluate the immune responses of the treated animals. Foot lesions of L. (L.) amazonensis-infected mice were injected with DPPE 1.2 alone, or associated with P. acnes as an adjuvant. Analysis of T-cell populations in the treated mice and in untreated controls was performed by FACS. Detection of IFN-gamma-secreting lymphocytes was carried out by an ELISPOT assay and active TGF-beta was measured by means of a double-sandwich ELISA test. The treatment with DPPE 1.2 resulted in a significant reduction of foot lesion sizes and parasite burdens in both mouse strains, and the lowest parasite burden was found in mice treated with DPPE 1.2 plus P. acnes. Mice treated with DPPE 1.2 alone displayed a significant increase of TCD4(+) and TCD8(+) lymphocytes and IFN-gamma secretion which were significantly higher in animals treated with DPPE 1.2 plus P. acnes. A significant reduction of active TGF-b was observed in mice treated with DPPE 1.2 alone or associated with P. acnes. Moreover, DPPE 1.2 associated to P. acnes was non-toxic to treated animals. The destruction of L. (L.) amazonensis by DPPE 1.2 was followed by host inflammatory responses which were exacerbated when the palladacycle complex was associated with P. acnes.Sao Paulo Research Foundation (FAPESP)FAPESPUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, Sao Paulo, BrazilUniv Fed Sao Paulo, Escola Paulista Med, Dept Farmacol, Sao Paulo, BrazilUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, Sao Paulo, BrazilUniv Fed Sao Paulo, Escola Paulista Med, Dept Farmacol, Sao Paulo, BrazilFAPESP: 2013/02133-8FAPESP: 2009/10809-6FAPESP: 2014/06935-4Web of Science-engFrontiers Media SaFrontiers In Microbiologycutaneous leishmaniasisLeishmania (Leishmania) amazonensispalladacycle complexadjuvantPropionibacterium acnesTreatment of Leishmania (Leishmania) Amazonensis-Infected Mice with a Combination of a Palladacycle Complex and Heat-Killed Propionibacterium acnes Triggers Protective Cellular Immune Responsesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLausanne8info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000395238500001.pdfapplication/pdf2081181${dspace.ui.url}/bitstream/11600/54937/1/WOS000395238500001.pdf69932c8450d789cbdea0c504d0079f14MD51open accessTEXTWOS000395238500001.pdf.txtWOS000395238500001.pdf.txtExtracted texttext/plain52448${dspace.ui.url}/bitstream/11600/54937/2/WOS000395238500001.pdf.txte5a73a15a999d224ef7478542399370dMD52open accessTHUMBNAILWOS000395238500001.pdf.jpgWOS000395238500001.pdf.jpgIM Thumbnailimage/jpeg7076${dspace.ui.url}/bitstream/11600/54937/4/WOS000395238500001.pdf.jpg70b26a0e9bf13df04d9b7ff948b4bdc6MD54open access11600/549372022-08-01 05:22:43.729open accessoai:repositorio.unifesp.br:11600/54937Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:27:20.933221Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Treatment of Leishmania (Leishmania) Amazonensis-Infected Mice with a Combination of a Palladacycle Complex and Heat-Killed Propionibacterium acnes Triggers Protective Cellular Immune Responses |
title |
Treatment of Leishmania (Leishmania) Amazonensis-Infected Mice with a Combination of a Palladacycle Complex and Heat-Killed Propionibacterium acnes Triggers Protective Cellular Immune Responses |
spellingShingle |
Treatment of Leishmania (Leishmania) Amazonensis-Infected Mice with a Combination of a Palladacycle Complex and Heat-Killed Propionibacterium acnes Triggers Protective Cellular Immune Responses Paladi, Carolina S. [UNIFESP] cutaneous leishmaniasis Leishmania (Leishmania) amazonensis palladacycle complex adjuvant Propionibacterium acnes |
title_short |
Treatment of Leishmania (Leishmania) Amazonensis-Infected Mice with a Combination of a Palladacycle Complex and Heat-Killed Propionibacterium acnes Triggers Protective Cellular Immune Responses |
title_full |
Treatment of Leishmania (Leishmania) Amazonensis-Infected Mice with a Combination of a Palladacycle Complex and Heat-Killed Propionibacterium acnes Triggers Protective Cellular Immune Responses |
title_fullStr |
Treatment of Leishmania (Leishmania) Amazonensis-Infected Mice with a Combination of a Palladacycle Complex and Heat-Killed Propionibacterium acnes Triggers Protective Cellular Immune Responses |
title_full_unstemmed |
Treatment of Leishmania (Leishmania) Amazonensis-Infected Mice with a Combination of a Palladacycle Complex and Heat-Killed Propionibacterium acnes Triggers Protective Cellular Immune Responses |
title_sort |
Treatment of Leishmania (Leishmania) Amazonensis-Infected Mice with a Combination of a Palladacycle Complex and Heat-Killed Propionibacterium acnes Triggers Protective Cellular Immune Responses |
author |
Paladi, Carolina S. [UNIFESP] |
author_facet |
Paladi, Carolina S. [UNIFESP] da Silva, Danielle A. M. [UNIFESP] Motta, Priscila D. [UNIFESP] Garcia, Daniel M. [UNIFESP] Teixeira, Daniela [UNIFESP] Longo-Maugeri, Ieda M. [UNIFESP] Katz, Simone [UNIFESP] Barbieri, Clara L. [UNIFESP] |
author_role |
author |
author2 |
da Silva, Danielle A. M. [UNIFESP] Motta, Priscila D. [UNIFESP] Garcia, Daniel M. [UNIFESP] Teixeira, Daniela [UNIFESP] Longo-Maugeri, Ieda M. [UNIFESP] Katz, Simone [UNIFESP] Barbieri, Clara L. [UNIFESP] |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Paladi, Carolina S. [UNIFESP] da Silva, Danielle A. M. [UNIFESP] Motta, Priscila D. [UNIFESP] Garcia, Daniel M. [UNIFESP] Teixeira, Daniela [UNIFESP] Longo-Maugeri, Ieda M. [UNIFESP] Katz, Simone [UNIFESP] Barbieri, Clara L. [UNIFESP] |
dc.subject.eng.fl_str_mv |
cutaneous leishmaniasis Leishmania (Leishmania) amazonensis palladacycle complex adjuvant Propionibacterium acnes |
topic |
cutaneous leishmaniasis Leishmania (Leishmania) amazonensis palladacycle complex adjuvant Propionibacterium acnes |
description |
Palladacycle complex DPPE 1.2 was previously reported to inhibit the in vitro and in vivo infection by Leishmania (Leishmania) amazonensis. The aim of the present study was to compare the effect of DPPE 1.2, in association with heat-killed Propionibacterium acnes, on L. (L.) amazonensis infection in two mouse strains, BALB/c and C57BL/6, and to evaluate the immune responses of the treated animals. Foot lesions of L. (L.) amazonensis-infected mice were injected with DPPE 1.2 alone, or associated with P. acnes as an adjuvant. Analysis of T-cell populations in the treated mice and in untreated controls was performed by FACS. Detection of IFN-gamma-secreting lymphocytes was carried out by an ELISPOT assay and active TGF-beta was measured by means of a double-sandwich ELISA test. The treatment with DPPE 1.2 resulted in a significant reduction of foot lesion sizes and parasite burdens in both mouse strains, and the lowest parasite burden was found in mice treated with DPPE 1.2 plus P. acnes. Mice treated with DPPE 1.2 alone displayed a significant increase of TCD4(+) and TCD8(+) lymphocytes and IFN-gamma secretion which were significantly higher in animals treated with DPPE 1.2 plus P. acnes. A significant reduction of active TGF-b was observed in mice treated with DPPE 1.2 alone or associated with P. acnes. Moreover, DPPE 1.2 associated to P. acnes was non-toxic to treated animals. The destruction of L. (L.) amazonensis by DPPE 1.2 was followed by host inflammatory responses which were exacerbated when the palladacycle complex was associated with P. acnes. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2020-07-17T14:02:40Z |
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2020-07-17T14:02:40Z |
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dc.identifier.citation.fl_str_mv |
Frontiers In Microbiology. Lausanne, v. 8, p. -, 2017. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/54937 http://dx.doi.org/10.3389/fmicb.2017.00333 |
dc.identifier.issn.none.fl_str_mv |
1664-302X |
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WOS000395238500001.pdf |
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10.3389/fmicb.2017.00333 |
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WOS:000395238500001 |
identifier_str_mv |
Frontiers In Microbiology. Lausanne, v. 8, p. -, 2017. 1664-302X WOS000395238500001.pdf 10.3389/fmicb.2017.00333 WOS:000395238500001 |
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https://repositorio.unifesp.br/handle/11600/54937 http://dx.doi.org/10.3389/fmicb.2017.00333 |
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Frontiers In Microbiology |
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