Occurrence of Helicobacter pylori and Epstein-Barr virus infection in endoscopic and gastric cancer patients from Northern Brazil

• Autor(a) principal: Souza, Carolina Rosal Teixeira de
• Data de Publicação: 2014
• Outros Autores: Oliveira, Katia Soares de, Ferraz, Jefferson Jose Sodre, Leal, Mariana Ferreira [UNIFESP], Calcagno, Danielle Queiroz [UNIFESP], Seabra, Aline Damasceno, Khayat, Andre Salim, Montenegro, Raquel Carvalho, Alves, Ana Paula Negreiros Nunes, Assumpcao, Paulo Pimentel, Smith, Marilia de Arruda Cardoso [UNIFESP], Burbano, Rommel Rodriguez
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://repositorio.unifesp.br/handle/11600/38330; http://dx.doi.org/10.1186/1471-230X-14-179
• topic: Helicobacter pylori; Epstein-Barr virus; Gastritis; Gastric cancer

Background: Helicobacter pylori (HP) and Epstein-Barr virus (EBV) have been associated with cancer development. We evaluated the prevalence of HP, HP CagA(+) and EBV infection in gastric cancer (GC) samples from adults and in gastric tissues from patients who underwent upper endoscopy (UE).Methods: Samples from UE and GC were collected to investigate the presence of HP infection and the HP virulence factor CagA by a urease test and PCR. the presence of EBV was detected by Eber-1 in situ hybridization.Results: in UE, 85.5% of juvenile patients showed some degree of gastritis (45.3% of patients with mild gastritis and 54.7% with moderate/severe gastritis) and patients with mild gastritis were younger than patients with moderate/severe gastritis. Among adults, 48.7% presented mild gastritis and 51.3% moderate/severe gastritis. HP infection was detected in 0% of normal mucosa, 58.5% of juvenile gastritis patients, 69.2% of adult gastritis patients and 88% of GC patients. in these same groups, HP CagA(+) was detected in 0%, 37.7%, 61.5% and 67.2% of tissue samples, respectively. in juvenile patients, HP infection was more common in those with gastritis than in normal samples (p = 0.004). the patients with either HP or HP CagA(+) were older than patients without these pathogens (p < 0.05). in juvenile patients, HP infection was more frequent in cases of moderate/severe gastritis than in cases of mild gastritis (p = 0.026). Moreover, in patients with GC, HP infection was more frequent in males than in females (p = 0.023). GC patients with HP CagA(+) were older than patients with HP CagA-(p = 0.027). HP CagA(+) was more common in intestinal-type than diffuse-type GC (p = 0.012). HP CagA(+) was also associated with lymph-node (p = 0.024) and distal (p = 0.005) metastasis. No association between EBV infection and HP infection or any clinicopathological variable was detected.Conclusions: Our results suggest that HP is involved in the pathophysiology of severe gastric lesions and in the development of GC, particularly when CagA(+) is present. EBV was not the primary pathogenic factor in our samples.